FastQCFastQC Report
Wed 25 May 2016
SRR1699889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372985
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN96770.2868972141886193No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN74910.2220881504068355No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69700.20664189138107641No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN63300.1876676000634453No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN61750.18307226388495648No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN58630.17382229686761133No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN57960.17183592574529682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN54040.16021417231324775No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN53820.1595619310492042No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN48680.1443232033347317No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN47800.14171423827855742No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN46440.13768220137356083No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN45780.1357254775814301No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN41950.12437055012103522No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN41460.12291783094202909No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN39650.11755166417876153No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN38500.1141422212076247No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN38430.1139346898963381No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN36970.10960617968950352No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN34070.1010084539362019No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG39450.030.258871
TACCTGG41200.028.460392
ACCTGGG41350.028.0907143
CCTGGGG31850.027.766494
TAACGCA2450.026.039724
CTGGGGG24850.025.6729625
GGTATCA46250.024.38141
TCGACTA602.852953E-422.0242141
GTATCAA91550.021.2904741
TACACGG2900.021.2407992
GTACACG3050.020.9410551
CAACGGT5050.020.9100139
CGACAAC5100.019.842336
GCGTAAT1705.456968E-1219.4331281
CGCGAAA7700.018.85746625
GTCTCGC2950.018.6645871
ACAACGG5450.018.56802638
CCGACAA5450.018.56802635
CGCGCAA20450.018.28773918
TCGCGCA20350.018.269517
Produced by FastQC (version 0.11.5)