FastQCFastQC Report
Wed 25 May 2016
SRR1699888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668269
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN65570.24573984107299526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN62070.23262272282142468No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN48750.1827027185040189No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN48030.18000433989226725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN47310.17730596128051557No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN42060.1576302839031597No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN38100.14278920153852553No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN35990.13488145310686442No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN35300.13229550693726908No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN32550.12198919973960647No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN30670.11494343336447713No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN29290.10977154102528643No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN28780.107860189508629No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN28520.10688577500994091No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN28000.1049369460125647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35000.032.2284551
TACCTGG37300.030.197542
ACCTGGG37550.029.8787613
CTGGGGG20000.028.158685
CCTGGGG29000.028.067654
GGTATCA35000.027.507491
GTACCGG1800.026.9269891
ACGCGTG450.001399037124.443334
ACCGGGG1700.023.2930263
CGACAAC2600.022.84551236
CAACGGT2800.021.99938239
AACGCGT500.002581949321.9989733
GTATCAA69050.021.7918761
TAACGCA2550.021.5676174
TATCACG2350.020.5951672
AGCGTCG4400.020.49942640
ACACGGG2150.020.4641553
GTCGGTA4600.020.08601643
TACACGG2450.019.7545452
CGCGAAA4750.019.45281425
Produced by FastQC (version 0.11.5)