FastQCFastQC Report
Wed 25 May 2016
SRR1699887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500325
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN118720.26380316977107204No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN110570.2456933665901907No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN79750.17720942376383927No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN77270.17169871064867537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN74150.16476587801992076No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN69080.15350002499819457No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN63520.14114536172387548No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN62910.13978990406248437No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN62280.13839000516629354No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN61130.13583463416531028No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN58820.13070167154594392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN58810.13067945092854405No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN58770.13059056845894462No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN58490.12996839117174872No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN57570.12792409437096208No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN53770.1194802597590174No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN53760.11945803914161755No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN50390.11196969107786658No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN49540.11008093859887896No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN46850.10410359251831812No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN46560.10345919461372233No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN45650.10143711843033558No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGN45570.10125935349113675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56000.031.5628721
TACCTGG58600.029.8816032
ACCTGGG58000.029.697663
CCTGGGG46500.027.9120884
CTGGGGG34250.027.554345
AACGCGT858.250936E-925.88146433
TAACGCA4400.023.498414
TCGCGAA15100.022.29186224
ATCGCGA15250.022.072623
CGCGAAA15100.022.00046525
GTACCGG4100.020.4263111
GCGTAAT2400.020.202341
ACGCGTG1201.4346369E-820.16597434
GATCGCG16800.020.0361422
GGTATCA66250.020.0263041
GAACGAT17150.019.6259318
AAACGGT6850.019.59165823
AGCGTCG16500.019.46578240
GTCGGTA16700.019.23201843
CGACAAC6300.019.20611636
Produced by FastQC (version 0.11.5)