FastQCFastQC Report
Wed 25 May 2016
SRR1699886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164614
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN116000.27853721857535896No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN93070.2234780942483505No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN75940.18234583085011No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN75270.18073703829454543No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN65870.15816591885826634No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN61500.1476727495033153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN59890.14380684500412283No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN58420.1402771061135558No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN57490.1380440059991154No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN55160.13244924979842068No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN52630.12637425701397537No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN52620.12635024518478782No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN50390.12099560727596842No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN48490.11643335973033757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN46830.1124473960852074No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN45890.1101902841415795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG53550.032.4098321
TACCTGG55050.031.0104712
ACCTGGG53650.030.9585913
CCTGGGG45900.027.9907514
TAACGCA3100.027.6768914
CTGGGGG33700.027.1567525
GGTATCA45000.024.0313971
GTACCGG2350.022.4933131
GTACACG2650.022.4402621
CAACGGT6650.021.83364539
GTATCAA90900.021.7339481
CGACAAC7150.020.61449636
CGATCGA7300.020.19236427
ATCGCGA7100.020.1416723
TCGCGAA7250.019.72494524
CGGTATA6300.019.20713426
ACGGTAT6300.019.20713425
CGCGAAA7450.019.19541525
ACGGTAA7800.018.89662241
CATCGGA6550.018.80812517
Produced by FastQC (version 0.11.5)