FastQCFastQC Report
Wed 25 May 2016
SRR1699885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412569
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN128620.2914855269118738No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN125160.2836442897550157No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN80890.18331724671047636No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN80690.18286399600776782No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN69740.15804852003447425No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN69680.15791254482366168No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN65090.14751044119650028No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN64580.14635465190459346No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN64470.14610536401810376No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN63920.1448589245856552No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN62150.14084765586668446No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN60370.1368137246125783No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN58630.13287044349901383No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN57850.13110276575845045No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN57250.12974301365032478No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN55310.12534648183405175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN53990.12235502719617529No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN52980.12006611114749707No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGN47890.10853088076356428No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN46840.10615131457434435No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN46470.1053128007743335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN45180.1023893337418633No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN44410.10064431853643535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58900.031.9922371
TACCTGG61750.030.0314712
ACCTGGG61250.029.8456423
TAACGCA3250.027.7518184
CCTGGGG49600.027.6753944
CTGGGGG35600.026.2621235
CGCGAAA14400.023.52845825
ATCGCGA14700.023.04828523
GGTATCA48550.022.8788991
TCGCGAA14750.022.82099524
GATCGCG16700.020.81497222
CGAAATG16450.020.72984727
CGTCGGT15950.020.68814342
AGCGTCG15800.020.60702140
GTCGGTA16000.020.34828243
GAACGAT16750.020.0942818
GTATCAA88800.020.0535931
TCGGTAG16250.020.03522944
ACGATCG17050.019.7416120
AACGATA34000.019.60644723
Produced by FastQC (version 0.11.5)