FastQCFastQC Report
Wed 25 May 2016
SRR1699879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214268
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN92040.28634824476366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN73600.2289790397067077No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN66560.20707669677824No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN56830.17680541883875273No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN56630.17618319318737577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN56560.17596541420939388No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN56260.17503207573232848No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN52710.1639875704203881No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN51440.16003643753414462No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN46530.14476079779284118No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN46490.14463635266256578No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN45380.14118300029742387No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN43480.1352718566093431No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN40400.12568958157813848No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN37360.11623175167720926No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN35250.10966727105518269No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN34000.10577836073407693No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN33860.10534280277811309No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN33830.10524946893040656No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN33680.10478279969187386No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN33370.10381834993223962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN32590.10139166989186962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG36150.030.3530371
CCTGGGG29250.028.2791924
ACCTGGG38300.028.145073
CTGGGGG21050.028.1128735
TACCTGG40400.027.0088022
GTACACG3500.025.1810071
TAACGCA3000.024.932324
TACACGG3350.024.2975182
GGTATCA54750.021.5303341
CGTTCGC550.00448625819.99918723
CGATCGA5750.019.5122527
GTATCAA99800.019.4944651
CGACAAC5750.019.13025336
ACGGTAA5950.018.85666841
GATCCGA6100.018.75362832
AACGATA17000.018.5051323
GCAACGA17200.018.41785621
CGCAACG17350.018.3854220
CAACGGT5900.018.27101139
CGCGCAA17500.018.22782918
Produced by FastQC (version 0.11.5)