FastQCFastQC Report
Wed 25 May 2016
SRR1699876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3609229
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN112910.3128368967444293No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN83540.23146217654795523No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN72210.20007043055455886No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN67180.18613393608441026No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN64710.17929036921735916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN61990.17175413363906808No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN59360.16446725879682336No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN57760.16003417904488743No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN55920.1549361373301611No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN54730.15163903426465875No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN54560.15116801954101555No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN48910.13551370666699175No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN48780.13515351893714697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN47680.13210577660769102No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN44570.12348897783986552No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN42790.1185571766158368No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN40920.11337601465576166No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN40500.11221233122087847No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN38340.10622767355576496No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN38080.10550729809607536No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN37280.1032907582201074No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN36760.10185000730072821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG42750.031.2246531
ACCTGGG44250.028.983883
TACCTGG45700.028.7863242
CCTGGGG38450.026.5474534
CTGGGGG26800.026.1856375
CGCGAAA7550.022.43815425
TCGCGAA7600.022.00104724
ACGCGTG803.6203928E-621.99891334
CATCGGA6850.021.83833717
ATCGCGA7800.021.43691623
GTCGGTA8050.021.31571643
TACACGG3200.021.3114452
CGGTATA6550.021.16131226
CGTCGGT8050.021.04243742
AACGCGT956.877344E-720.84107633
GGTATCA50350.020.8242131
GATCGCG8050.020.77117322
AGCGTCG8200.020.65751540
ACGGTAT6850.020.55572125
GTATCAA90850.020.2694721
Produced by FastQC (version 0.11.5)