FastQCFastQC Report
Wed 25 May 2016
SRR1699875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973425
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN219660.4416674625635252No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN165170.33210513881278997No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN131550.2645058485852305No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN108830.21882304448141873No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN107010.215163594504793No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN100170.2014104967904412No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN99490.20004322976620736No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN98770.19859553526995985No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN95710.19244283366090773No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN91700.18437997959152896No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN91540.18405826970347397No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN89660.18027817851882758No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN89550.18005700297078975No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN88290.1775235376023565No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN85460.1718332939573835No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN85290.17149147720132504No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN80730.16232274539175717No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN76970.15476256302246438No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGN73160.1471018463131544No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN71840.14444773973670055No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACN66860.1344345194709883No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN66650.13401227524291612No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN63100.12687433710169552No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAN60460.12156612394878781No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN59350.11933426160040615No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN57730.11607694898384917No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCN57070.11474989569562222No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCN56840.11428743773154316No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAAN54260.10909986578665609No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGN53710.10799398804646697No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACN52160.10487742350593404No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN51420.10338951527367961No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTN50130.10079572930123608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG58000.031.2658861
ACCTGGG61750.028.4640373
TACCTGG63400.028.174322
CCTGGGG51900.027.2963874
CTGGGGG39900.027.1807045
GCGTAAT2450.024.2826791
CGCGAAA19850.022.9429325
TCGCGAA19950.022.9382124
ATCGCGA20200.022.8721523
AGCGTCG19750.022.83561540
GTCGGTA19750.022.72216843
AATCGCG51650.022.61574415
AACGATA52800.022.54251723
TCGCGCA52550.022.39585917
GATCGCG20750.022.3719322
CGCGCAA52500.022.33338518
CGTCGGT20100.022.3267342
CGCAACG53750.022.14342120
GCGCAAC53750.022.10093519
TCGGTAG20550.021.83760544
Produced by FastQC (version 0.11.5)