##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660780_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28900209 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.091384287220897 33.0 33.0 33.0 14.0 33.0 2 30.5372448690596 33.0 33.0 33.0 27.0 33.0 3 29.95816075240148 33.0 33.0 33.0 14.0 33.0 4 30.12584718677986 33.0 33.0 33.0 14.0 33.0 5 30.11546065289701 33.0 33.0 33.0 14.0 33.0 6 33.45291271076967 37.0 33.0 37.0 14.0 37.0 7 33.62097426354252 37.0 33.0 37.0 27.0 37.0 8 34.3532203867453 37.0 37.0 37.0 27.0 37.0 9 32.427745453328725 37.0 33.0 37.0 14.0 37.0 10-11 33.84901211268057 37.0 35.0 37.0 27.0 37.0 12-13 33.426081070901596 37.0 33.0 37.0 14.0 37.0 14-15 33.06617322386838 37.0 33.0 37.0 14.0 37.0 16-17 33.03145082791616 37.0 33.0 37.0 14.0 37.0 18-19 33.28784693217962 37.0 33.0 37.0 14.0 37.0 20-21 33.23589618331134 37.0 33.0 37.0 14.0 37.0 22-23 33.872334815986974 37.0 37.0 37.0 27.0 37.0 24-25 34.00238060562123 37.0 37.0 37.0 27.0 37.0 26-27 34.2295932012118 37.0 37.0 37.0 27.0 37.0 28-29 34.127876428160086 37.0 37.0 37.0 27.0 37.0 30-31 33.5986074010745 37.0 35.0 37.0 18.0 37.0 32-33 33.099043903108104 37.0 33.0 37.0 14.0 37.0 34-35 33.393994815054796 37.0 35.0 37.0 20.5 37.0 36-37 33.44700578116927 37.0 35.0 37.0 18.0 37.0 38-39 33.10564023948754 37.0 33.0 37.0 14.0 37.0 40-41 33.69132859904231 37.0 37.0 37.0 27.0 37.0 42-43 33.82615234720275 37.0 37.0 37.0 27.0 37.0 44-45 33.75027102745174 37.0 35.0 37.0 20.5 37.0 46-47 33.222137130565386 37.0 33.0 37.0 18.0 37.0 48-49 33.553856928854735 37.0 33.0 37.0 18.0 37.0 50-51 33.556264125979155 37.0 35.0 37.0 18.0 37.0 52-53 33.140198657386875 37.0 33.0 37.0 14.0 37.0 54-55 33.64258376124546 37.0 35.0 37.0 20.5 37.0 56-57 33.81319647203935 37.0 37.0 37.0 24.5 37.0 58-59 33.74153520827479 37.0 37.0 37.0 20.5 37.0 60-61 34.051260996070994 37.0 37.0 37.0 27.0 37.0 62-63 34.31617520482291 37.0 37.0 37.0 27.0 37.0 64-65 34.23493300688587 37.0 37.0 37.0 27.0 37.0 66-67 34.448601842291176 37.0 37.0 37.0 27.0 37.0 68-69 32.567584320929996 37.0 30.0 37.0 14.0 37.0 70-71 31.532071965292708 37.0 27.0 37.0 14.0 37.0 72-73 32.03963379641995 37.0 27.0 37.0 14.0 37.0 74-75 32.07971080762772 37.0 30.0 37.0 14.0 37.0 76-77 31.727764581218082 37.0 27.0 37.0 14.0 37.0 78-79 31.447956777752022 37.0 27.0 37.0 14.0 37.0 80-81 31.465213071642495 37.0 27.0 37.0 14.0 37.0 82-83 31.358152306095782 37.0 27.0 37.0 14.0 37.0 84-85 31.25073958807703 37.0 27.0 37.0 14.0 37.0 86-87 31.237905321030723 37.0 27.0 37.0 14.0 37.0 88-89 31.185721874883328 37.0 27.0 37.0 14.0 37.0 90-91 31.30677210673459 37.0 27.0 37.0 14.0 37.0 92-93 31.15479401896367 37.0 27.0 37.0 14.0 37.0 94-95 31.208469582348002 37.0 27.0 37.0 14.0 37.0 96-97 31.049103139703938 37.0 24.5 37.0 14.0 37.0 98-99 30.968943321482556 37.0 22.0 37.0 14.0 37.0 100 31.048097887458184 37.0 22.0 37.0 14.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.506368123544533 1101 2 0.6730988136352352 1101 3 0.5892341660093123 1101 4 0.5800290848640053 1101 5 0.4171866280255081 1101 6 1.1347304846441446 1101 7 0.6723004314652457 1101 8 0.6404631732215478 1101 9 2.092198957803525 1101 10-11 0.679184941953217 1101 12-13 0.7826483203401509 1101 14-15 0.6172778177841423 1101 16-17 0.5685643079750662 1101 18-19 0.4258505245872328 1101 20-21 1.0291123073043664 1101 22-23 0.9563191924506143 1101 24-25 0.4377311440519307 1101 26-27 0.35822404615053927 1101 28-29 0.10343719881839775 1101 30-31 0.25041775156025636 1101 32-33 0.6154133347911923 1101 34-35 0.830088034994624 1101 36-37 0.69053117357155 1101 38-39 0.7359005929121523 1101 40-41 0.7863484461215009 1101 42-43 0.3526701555402951 1101 44-45 0.3347181665168719 1101 46-47 0.28317675314364976 1101 48-49 0.3305784284267048 1101 50-51 0.2552578602455995 1101 52-53 0.24516057002947633 1101 54-55 0.19868118807244173 1101 56-57 0.2803651020393616 1101 58-59 0.14681913481360453 1101 60-61 0.2047820524086248 1101 62-63 0.31725853022447126 1101 64-65 0.20272261008357617 1101 66-67 0.14598050408671526 1101 68-69 0.3990814502486728 1101 70-71 0.23619189798840168 1101 72-73 0.11652506800916029 1101 74-75 0.13653103098140207 1101 76-77 0.2724448280556544 1101 78-79 0.2918539487772769 1101 80-81 0.19338728158590612 1101 82-83 0.22582328555302666 1101 84-85 0.29124344735710395 1101 86-87 0.28122786389922894 1101 88-89 0.2795225219981887 1101 90-91 0.23927905674336714 1101 92-93 0.25373399965067733 1101 94-95 0.14227749808493684 1101 96-97 0.2279675911778014 1101 98-99 0.3170901770480299 1101 100 0.25224333073626326 1102 1 0.61181043335041 1102 2 0.6960940518734482 1102 3 0.6693879391584687 1102 4 0.7290096876733436 1102 5 0.5141573731028544 1102 6 1.0516606815100928 1102 7 0.7401501832718793 1102 8 0.626024649986654 1102 9 2.1259704700245123 1102 10-11 0.6954790772160422 1102 12-13 0.89389817242796 1102 14-15 0.7686671156894391 1102 16-17 0.6695717631600218 1102 18-19 0.5021192879103964 1102 20-21 1.0503282682315742 1102 22-23 0.8732283032662949 1102 24-25 0.45196483369967666 1102 26-27 0.38507494241414264 1102 28-29 0.1756303345143806 1102 30-31 0.4348867593856198 1102 32-33 0.854995204494962 1102 34-35 1.0850739248835382 1102 36-37 0.8760042743691017 1102 38-39 0.8675452008351883 1102 40-41 0.9091099327607708 1102 42-43 0.6035782977815032 1102 44-45 0.475640606269657 1102 46-47 0.4596147365048324 1102 48-49 0.5334365035736894 1102 50-51 0.5258615592462164 1102 52-53 0.5143860926126749 1102 54-55 0.33612912619266666 1102 56-57 0.3310524630137692 1102 58-59 0.2040720269456031 1102 60-61 0.13219146411812233 1102 62-63 0.23970125337377368 1102 64-65 0.1370132170172198 1102 66-67 0.2201351503302149 1102 68-69 0.31760458859994145 1102 70-71 0.3479411500311862 1102 72-73 0.23720652563499556 1102 74-75 0.22936861899556504 1102 76-77 0.36729195556576144 1102 78-79 0.3460965538801126 1102 80-81 0.26180158320892843 1102 82-83 0.29692828227645407 1102 84-85 0.30909145515564873 1102 86-87 0.37921227232711985 1102 88-89 0.3372875015925949 1102 90-91 0.2894534822471613 1102 92-93 0.3080789551508403 1102 94-95 0.23538422386914704 1102 96-97 0.2764253871418454 1102 98-99 0.3471650257936503 1102 100 0.1999741921648095 1103 1 0.5535803071174961 1103 2 0.23520303338510473 1103 3 0.5426098569400253 1103 4 0.6329557668326018 1103 5 0.5059170381556086 1103 6 1.014191665824967 1103 7 0.6932720783970296 1103 8 0.516542289595435 1103 9 2.0853700719704094 1103 10-11 0.7051444119400472 1103 12-13 0.7543187474093642 1103 14-15 0.614880062982742 1103 16-17 0.6467845905438026 1103 18-19 0.5228649646755414 1103 20-21 1.0655378986621358 1103 22-23 0.8610759739801495 1103 24-25 0.5101701628797244 1103 26-27 0.43914603358159354 1103 28-29 0.24885763955529683 1103 30-31 0.34154680735278475 1103 32-33 0.8135464797108511 1103 34-35 1.0311594552669803 1103 36-37 0.8197971155249562 1103 38-39 0.8332428440707034 1103 40-41 0.766462915855783 1103 42-43 0.5737331349512971 1103 44-45 0.4291465003387387 1103 46-47 0.43911638243631046 1103 48-49 0.46569987332151896 1103 50-51 0.41133236824227737 1103 52-53 0.4686441220060473 1103 54-55 0.32649786323916885 1103 56-57 0.3677074334691852 1103 58-59 0.2860831397538206 1103 60-61 0.2043716046005457 1103 62-63 0.31513826222271035 1103 64-65 0.186541868409698 1103 66-67 0.23856237838817407 1103 68-69 0.28424263926719817 1103 70-71 0.3150033512466237 1103 72-73 0.2633447958846844 1103 74-75 0.3057453804532102 1103 76-77 0.38522145986314626 1103 78-79 0.32003100161131925 1103 80-81 0.3092874081313042 1103 82-83 0.3485849034451789 1103 84-85 0.4226902335178053 1103 86-87 0.3929302368698053 1103 88-89 0.38574847918288313 1103 90-91 0.4026423786782445 1103 92-93 0.37487386599488914 1103 94-95 0.29201069083352493 1103 96-97 0.27661507142989805 1103 98-99 0.39451911010991125 1103 100 0.3771703106087898 1104 1 0.46076663752917213 1104 2 0.6688478780754963 1104 3 0.5995777151058377 1104 4 0.5205482104034935 1104 5 0.38463769563154315 1104 6 0.9294651077573803 1104 7 0.5978089503367059 1104 8 0.4364656354892844 1104 9 2.1295883512591374 1104 10-11 0.6262535652253263 1104 12-13 0.7847202756793266 1104 14-15 0.6445333459090463 1104 16-17 0.5947579715457039 1104 18-19 0.41376428586966796 1104 20-21 1.002009434524716 1104 22-23 0.8691401241373669 1104 24-25 0.47089305469256715 1104 26-27 0.3592274527100159 1104 28-29 0.17033327479941818 1104 30-31 0.2847130441036825 1104 32-33 0.6395912987003882 1104 34-35 0.8853355151575428 1104 36-37 0.7072938973230904 1104 38-39 0.6554397120904198 1104 40-41 0.759560118309146 1104 42-43 0.510180412155826 1104 44-45 0.36646703962600924 1104 46-47 0.34294319471548107 1104 48-49 0.34963248920571033 1104 50-51 0.3899043180388446 1104 52-53 0.4279866930025378 1104 54-55 0.28539664932726794 1104 56-57 0.3784008206855489 1104 58-59 0.21732739713444715 1104 60-61 0.15345195336463036 1104 62-63 0.24705210125143395 1104 64-65 0.028412344875142992 1104 66-67 0.12436340990265649 1104 68-69 0.09433466879981722 1104 70-71 0.10702913238510448 1104 72-73 0.08063618657294569 1104 74-75 0.16701299222741994 1104 76-77 0.19021425025110972 1104 78-79 0.19288927734289274 1104 80-81 0.12197382831876524 1104 82-83 0.13078097942879552 1104 84-85 0.18838496345998124 1104 86-87 0.2309380698846475 1104 88-89 0.1334946828560426 1104 90-91 0.11174801489130815 1104 92-93 0.13338343868574754 1104 94-95 0.1382975634342536 1104 96-97 0.1336299878736753 1104 98-99 0.2146340217032332 1104 100 0.12904007371798087 1105 1 0.5107360096515556 1105 2 0.417464269598554 1105 3 0.542499790208506 1105 4 0.5500968928790293 1105 5 0.42098582722457323 1105 6 0.894999256496348 1105 7 0.7013559474094535 1105 8 0.45967815842795545 1105 9 2.1510320879253797 1105 10-11 0.8160013053844821 1105 12-13 0.8434285580515493 1105 14-15 0.644165881165506 1105 16-17 0.6319562617676127 1105 18-19 0.5371445358381735 1105 20-21 1.126170345386889 1105 22-23 0.8879197964119285 1105 24-25 0.5608816129562584 1105 26-27 0.4659355077841951 1105 28-29 0.325141856109461 1105 30-31 0.46886479395782743 1105 32-33 0.8025263814454391 1105 34-35 1.0080259330732204 1105 36-37 0.818009280362169 1105 38-39 0.7550352652112693 1105 40-41 0.7822874878211863 1105 42-43 0.6293916734702805 1105 44-45 0.4700208571328588 1105 46-47 0.43898701864912226 1105 48-49 0.5013624990480494 1105 50-51 0.5315467587893536 1105 52-53 0.5251554089851354 1105 54-55 0.43713271725336966 1105 56-57 0.4930559064632192 1105 58-59 0.37551390871887946 1105 60-61 0.27349643589658257 1105 62-63 0.3171711446446679 1105 64-65 0.18753086708429123 1105 66-67 0.18834123262434588 1105 68-69 0.11292165589315317 1105 70-71 0.17797410772757516 1105 72-73 0.19886209253236586 1105 74-75 0.16002231646098863 1105 76-77 0.22503935628536453 1105 78-79 0.18488394380413453 1105 80-81 0.1705870877582214 1105 82-83 0.15078041395486252 1105 84-85 0.22941963476127114 1105 86-87 0.2754125852799909 1105 88-89 0.23638020810062343 1105 90-91 0.28172873727926984 1105 92-93 0.2846725063316953 1105 94-95 0.08707433650402407 1105 96-97 0.21790622784282476 1105 98-99 0.28656984663932406 1105 100 0.28775662465064755 1106 1 0.39391957481562656 1106 2 0.5562824718719099 1106 3 0.3967832936525184 1106 4 0.4774190555037734 1106 5 0.3357060007204993 1106 6 1.011011646613703 1106 7 0.6058995454912477 1106 8 0.49965992143564364 1106 9 2.1406445307472097 1106 10-11 0.7532587359694318 1106 12-13 0.5651337893097974 1106 14-15 0.41312385714470423 1106 16-17 0.5132929388495668 1106 18-19 0.41440122123633927 1106 20-21 1.005170127392553 1106 22-23 0.8017689178243543 1106 24-25 0.5151493357569308 1106 26-27 0.38343963469094433 1106 28-29 0.2710115794310255 1106 30-31 0.4203592047949769 1106 32-33 0.7404901878428731 1106 34-35 1.0049844438753937 1106 36-37 0.7913045104292209 1106 38-39 0.643481592814048 1106 40-41 0.7681575534423644 1106 42-43 0.6233765941246787 1106 44-45 0.4538174425411228 1106 46-47 0.41446558251902843 1106 48-49 0.3630278201602124 1106 50-51 0.4737121299903677 1106 52-53 0.4974773555231735 1106 54-55 0.33570120900700573 1106 56-57 0.35386755747403953 1106 58-59 0.3447349002342932 1106 60-61 0.22636440619545084 1106 62-63 0.22669796440336398 1106 64-65 0.08926425490344769 1106 66-67 0.1893037487618301 1106 68-69 -0.05533345962873426 1106 70-71 0.07103786796158928 1106 72-73 0.14182974691208017 1106 74-75 0.13456762523478716 1106 76-77 0.04631139919457894 1106 78-79 0.09278708391001089 1106 80-81 0.05604942389958012 1106 82-83 0.09870116919085348 1106 84-85 0.14085400909515045 1106 86-87 0.18691874950080845 1106 88-89 0.17239244325620717 1106 90-91 0.1677118292772768 1106 92-93 0.14375468454154827 1106 94-95 0.12287334909593284 1106 96-97 0.0879783191898511 1106 98-99 0.21263998261999006 1106 100 0.2148067074494051 1107 1 0.2300389602972892 1107 2 0.13906289446867603 1107 3 0.373439103393693 1107 4 0.17195454775336216 1107 5 0.18851248442787494 1107 6 0.8078829478070588 1107 7 0.5818046926848552 1107 8 0.18080384716847675 1107 9 2.018246097267834 1107 10-11 0.6823039675383029 1107 12-13 0.6616520303168372 1107 14-15 0.49398576716965437 1107 16-17 0.4549926428722131 1107 18-19 0.4047477538525186 1107 20-21 0.9954439089476423 1107 22-23 0.7192701080897663 1107 24-25 0.35247699946791045 1107 26-27 0.3202695387785255 1107 28-29 0.20637155266336293 1107 30-31 0.39463007612056344 1107 32-33 0.6735616517618439 1107 34-35 0.9386073036841154 1107 36-37 0.800293380944872 1107 38-39 0.7288254535544496 1107 40-41 0.809298661793953 1107 42-43 0.7509266257904841 1107 44-45 0.5586785245077195 1107 46-47 0.4952167701021466 1107 48-49 0.5083686687772015 1107 50-51 0.5548903624105961 1107 52-53 0.5684152635589399 1107 54-55 0.47407944921423706 1107 56-57 0.4837383595128486 1107 58-59 0.28296874664608396 1107 60-61 0.07881879407435122 1107 62-63 0.2431670165472397 1107 64-65 0.14343738330202171 1107 66-67 0.25911909351136586 1107 68-69 0.09125060723450673 1107 70-71 0.2214049482472369 1107 72-73 0.243434162092953 1107 74-75 0.23124483496805937 1107 76-77 0.23661227663260576 1107 78-79 0.2092154893902034 1107 80-81 0.17654507950597775 1107 82-83 0.09074146756336532 1107 84-85 0.24220832924653024 1107 86-87 0.23221518454615975 1107 88-89 0.194979869825886 1107 90-91 0.21923194147922587 1107 92-93 0.23748554394263977 1107 94-95 0.14116888561499508 1107 96-97 0.1255345099856484 1107 98-99 0.197992412683476 1107 100 0.29588058746720236 1108 1 0.45809424376371055 1108 2 0.5053482958152316 1108 3 0.4487322984086859 1108 4 0.44284726873130253 1108 5 0.39516819498369316 1108 6 0.8334083155186747 1108 7 0.5875772276977642 1108 8 -0.1954101080828039 1108 9 1.9714812357971354 1108 10-11 0.6853767519528944 1108 12-13 0.4475483485175502 1108 14-15 0.40529659283474473 1108 16-17 0.4993979145897356 1108 18-19 0.4282790939425709 1108 20-21 0.9859615931695913 1108 22-23 0.7034715948626911 1108 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-0.7398117375981315 2316 80-81 -0.6540873069299309 2316 82-83 -0.8198491504811543 2316 84-85 -0.6438388723290842 2316 86-87 -0.7396257692084696 2316 88-89 -0.7351570059858332 2316 90-91 -0.814090847421074 2316 92-93 -0.7290558451252451 2316 94-95 -0.7656153747598893 2316 96-97 -0.9677690230707832 2316 98-99 -0.9807736257453072 2316 100 -0.7826615602974449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 50.0 6 132.0 7 229.0 8 336.0 9 360.0 10 388.0 11 446.0 12 578.0 13 639.0 14 5201.0 15 35652.0 16 48380.0 17 69916.0 18 148752.0 19 214975.0 20 322411.0 21 476119.0 22 566338.0 23 611518.0 24 651955.0 25 691180.0 26 747272.0 27 848779.0 28 1020434.0 29 1615506.0 30 1038386.0 31 721956.0 32 862544.0 33 1103964.0 34 1596894.0 35 2710449.0 36 1.2788462E7 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.20216774437505 19.30073672914185 12.98789179285106 31.509203733632035 2 28.24894443225646 27.162721797257632 24.233451237649287 20.35488253283662 3 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19.238757759718343 22-23 38.16773513609121 21.65889883759935 20.957100106764464 19.216265919544977 24-25 23.722493563788206 35.6232576280242 21.36585438610343 19.288394422084167 26-27 25.566888442042472 33.88404555341765 21.25177406371664 19.297291940823243 28-29 29.055426438184234 29.758237186294362 20.768648091230276 20.41768828429113 30-31 30.885319274903257 20.99865576385049 28.574759282060057 19.541265679186196 32-33 26.400570548298884 25.927430360398247 28.170372407511906 19.50162668379096 34-35 29.893918930498685 29.756248459038115 20.681695244738158 19.668137365725038 36-37 20.31849319844019 24.357299630601286 29.388398194628973 25.93580897632955 38-39 23.244916671709884 20.574432524000088 27.982199021467284 28.198451782822747 40-41 34.986110135427914 21.64543077707428 20.445157292109347 22.923301795388454 42-43 30.32527723242417 21.03085309867482 29.005227955271877 19.63864171362913 44-45 32.851122287731556 21.26077704143939 25.648966760067378 20.239133910761684 46-47 27.009945436460637 21.13922692145718 23.807982321073425 28.04284532100876 48-49 24.397427275209356 20.88766364202801 20.767160367808952 33.94774871495368 50-51 30.42377356790641 21.015730418842544 25.327450525155847 23.233045488095197 52-53 21.425342938714188 29.058009103925713 29.21930542712609 20.297342530234012 54-55 20.507181150540347 25.72369916522147 24.434908980848597 29.334210703389584 56-57 20.90300015301842 24.099568648705038 34.481291182560604 20.51614001571594 58-59 20.630585090672707 34.47868759073105 24.70526669622737 20.185460622368872 60-61 20.921279228315026 38.229914249511886 20.484503752182185 20.3643027699909 62-63 20.51737381636901 38.57574466261163 20.535990338381687 20.37089118263767 64-65 20.450559273419998 38.25711044279241 20.406638142628488 20.885692141159108 66-67 20.815891808882174 38.50753152430109 20.625514734084398 20.051061932732335 68-69 20.55565732947448 36.84950694935106 21.17408315857016 21.4207525626043 70-71 25.52231787681758 31.20895155329252 20.985899989979373 22.282830579910527 72-73 23.720516672089047 34.19771152475028 21.550050878079507 20.531720925081164 74-75 24.823745506030424 33.463212959300684 21.118362114997165 20.594679419671728 76-77 26.925338225664174 29.84065714713717 21.042618744638027 22.19138588256063 78-79 26.265665079797945 27.66005617487579 21.467954503610944 24.606324241715313 80-81 23.973488855065856 30.245039062499323 22.15605525885755 23.625416823577268 82-83 25.9361394273031 28.770373753891526 22.74687802973179 22.54660878907358 84-85 26.14336599834971 28.57895505694767 23.019187450037958 22.258491494664668 86-87 28.54372488863126 28.405837188291322 21.871806494501687 21.17863142857573 88-89 27.576103424824815 29.557284551937357 21.76984824012753 21.0967637831103 90-91 22.66786116939514 33.4811890135123 22.365855058630043 21.485094758462516 92-93 24.74799879820589 30.08271853303584 22.279769153074373 22.8895135156839 94-95 27.99567232344822 28.122739367405185 22.31326644736717 21.568321861779427 96-97 28.622951469942144 24.74983557970972 25.223716645162202 21.403496305185936 98-99 26.069450508430748 28.06926708744804 24.301310656975435 21.55997174714578 100 25.022989947213553 31.402590831276406 22.037773815056738 21.5366454064533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19004.0 1 13729.0 2 7102.0 3 5229.5 4 4403.5 5 3926.5 6 3727.0 7 3690.5 8 3752.5 9 3871.0 10 4016.0 11 4268.5 12 4573.0 13 4913.0 14 5457.5 15 6109.5 16 6838.5 17 7959.0 18 9374.0 19 11190.5 20 14073.0 21 18176.5 22 22700.5 23 28169.0 24 35654.5 25 44790.0 26 55836.5 27 69694.0 28 86537.0 29 108775.0 30 136438.0 31 171196.0 32 212941.5 33 261072.0 34 318169.5 35 392499.5 36 484295.0 37 584131.5 38 682408.5 39 771617.5 40 855232.0 41 924486.5 42 982726.0 43 1036865.5 44 1086850.5 45 1131436.5 46 1151473.5 47 1155129.0 48 1156272.0 49 1145538.5 50 1121084.5 51 1089936.5 52 1061925.0 53 1033432.0 54 997344.0 55 955295.0 56 904472.5 57 846495.0 58 786621.0 59 716846.5 60 636983.5 61 557969.0 62 479446.5 63 402396.0 64 332314.5 65 272993.5 66 225175.0 67 184655.5 68 150990.0 69 125393.5 70 106342.5 71 91720.0 72 81127.0 73 72781.5 74 64838.5 75 57504.0 76 50924.5 77 46098.0 78 40679.0 79 34497.5 80 29729.5 81 25434.0 82 20481.0 83 16133.5 84 11891.5 85 7903.5 86 5213.5 87 3029.5 88 1543.0 89 764.5 90 382.0 91 181.0 92 116.5 93 88.5 94 60.0 95 45.0 96 36.0 97 28.0 98 16.5 99 11.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010622760548202265 2 0.003698935187631342 3 0.0033632974764992185 4 0.005889230766462623 5 0.0057923456539708765 6 0.006543205275781915 7 0.003643572266207487 8 0.00519373406607544 9 3.890065293299436 10-11 0.004411732800963481 12-13 0.005216225252903881 14-15 0.0062092976559442875 16-17 0.00652244418024797 18-19 0.01186842628023901 20-21 1.4472680803104228 22-23 0.013460110271174856 24-25 0.011621023225126157 26-27 0.012555272524153718 28-29 0.015397812521009796 30-31 0.01597393292207679 32-33 0.0058528988492782185 34-35 0.9677871187713556 36-37 0.0 38-39 0.0 40-41 0.5032108937343671 42-43 0.0 44-45 0.0 46-47 0.21229604256495171 48-49 0.22310565297295948 50-51 0.14570482863982057 52-53 0.11149746356505588 54-55 0.058544559314432636 56-57 0.0625099285614163 58-59 0.06755314468487061 60-61 0.061416510863295144 62-63 0.05973313203375104 64-65 0.05749958417255737 66-67 0.04611558345477709 68-69 0.034330201556673864 70-71 0.03392363010246743 72-73 0.026328529319632258 74-75 0.024376986339441353 76-77 0.017953157362979626 78-79 0.016363203463338277 80-81 0.017441050339808962 82-83 0.021992920535626577 84-85 0.03080427549849207 86-87 0.03471428182405186 88-89 0.04051700802578971 90-91 0.029098405482119526 92-93 0.03484057848855003 94-95 0.035785208335344564 96-97 0.03221429990350589 98-99 0.035932266095376676 100 0.036013580386217964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.8900209E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.48753495989819 #Duplication Level Percentage of deduplicated Percentage of total 1 70.63247594179097 33.54162170589967 2 17.32136976383454 16.450983044268323 3 5.706401491200741 8.12948820925831 4 2.3090660568989714 4.3860742040681675 5 1.1741752310386064 2.7879343666496177 6 0.6797001808314055 1.9366371659688297 7 0.4597003411037683 1.5281025213469623 8 0.3133863331615733 1.1905555561571615 9 0.22344009870029236 0.9549557548625912 >10 1.084815149585051 9.0123860431982 >50 0.05976835514922233 1.9458715382652203 >100 0.031360433677567606 2.752110327925462 >500 0.002321104121723661 0.747297036485709 >1k 0.0015680983522833875 1.3968093072472985 >5k 1.62807412650322E-4 0.5770553836011147 >10k+ 2.8861314058897423E-4 12.662117834797304 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 1524333 5.2744705064243655 Illumina Single End PCR Primer 1 (100% over 50bp) AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 1023175 3.540372320490831 Illumina Single End PCR Primer 1 (100% over 50bp) AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 152433 0.5274460125876598 Illumina Single End PCR Primer 1 (98% over 50bp) AAGCAGAAGACGGCATACGAGATATATCAGTGTGACTGGAAAGAGTGTAG 101325 0.35060300082950957 Illumina PCR Primer Index 2 (96% over 28bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCCG 81647 0.2825135278433454 Illumina Single End PCR Primer 1 (98% over 50bp) AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCGCGGTGGTCGCC 58575 0.2026801951501458 Illumina Single End PCR Primer 1 (97% over 38bp) CAAGCAGAAGACGGCATACGAGATATATCAGTGTGACTGGAAAGAGTGTA 52672 0.18225473732733213 Illumina PCR Primer Index 2 (96% over 29bp) AAGCAGAAGACGGCATACGAGATATATCAGTGTGACTGGAGTTCAGACGT 36726 0.1270786657632822 TruSeq Adapter, Index 25 (96% over 30bp) GAGCGGAAGAGCGTAGTGGAGGGAAAGAGGGGAGATCGCGGTGGTCGCCG 36269 0.12549736232011333 No Hit GATCGTAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 32492 0.11242825268149444 Illumina Single End PCR Primer 1 (98% over 50bp) GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 30029 0.1039058229648097 Illumina Single End PCR Primer 1 (98% over 50bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.038171488656016 0.0 0.0 1.0380547766972896E-5 1.0380547766972896E-5 2 4.0553063128366995 1.0380547766972896E-5 0.0 1.3840730355963861E-5 3.460182588990965E-5 3 4.055780357851392 1.3840730355963861E-5 0.0 3.114164330091869E-5 4.8442556245873514E-5 4 4.056818412628089 1.3840730355963861E-5 0.0 3.460182588990965E-5 6.574346919082834E-5 5 4.05876649542569 1.3840730355963861E-5 0.0 3.460182588990965E-5 1.2456657320367476E-4 6 4.060780321692484 1.3840730355963861E-5 0.0 5.8823104012846414E-5 1.384073035596386E-4 7 4.063143626400764 1.7300912944954827E-5 0.0 6.92036517798193E-5 1.903100423945031E-4 8 4.077762897839251 3.460182588990965E-5 0.0 7.95841995467922E-5 1.9723040757248502E-4 9 4.084129633802994 3.806200847890062E-5 0.0 1.1764620802569283E-4 2.00690590161476E-4 10-11 4.087686701504477 4.671246495137803E-5 0.0 1.211063906146838E-4 2.2145168569542178E-4 12-13 4.094203955410842 1.2283648190917928E-4 0.0 1.3667721226514313E-4 2.993057939477185E-4 14-15 4.100375191058307 1.8338967721652117E-4 0.0 1.384073035596386E-4 3.54668715371574E-4 16-17 4.106215979268524 1.8684985980551213E-4 0.0 1.4186748614862957E-4 3.7542981090551975E-4 18-19 4.111539470181686 1.8857995110000761E-4 0.0 1.4186748614862957E-4 4.1003163679542937E-4 20-21 4.117501364782518 1.903100423945031E-4 0.0 1.4186748614862957E-4 4.4463346268533904E-4 22-23 4.12246672679772 1.9204013368899858E-4 0.0 1.4186748614862957E-4 4.4809364527433E-4 24-25 4.127864611636546 2.00690590161476E-4 0.0 1.4359757744312506E-4 4.8615565375323064E-4 26-27 4.132714057535017 2.0761095533945793E-4 0.0 1.4532766873762054E-4 6.038018617789236E-4 28-29 4.137857618953552 2.4740305511285405E-4 0.0 1.5570821650459345E-4 6.297532311963557E-4 30-31 4.143182839958008 2.6643405935230434E-4 0.0 1.591683990935844E-4 6.41863870257824E-4 32-33 4.149724315142496 2.7335442453028626E-4 0.0 1.6608876427156633E-4 6.59164783202779E-4 34-35 4.155838457777243 2.8200488100276367E-4 0.0 1.7646931203853925E-4 6.868462439147066E-4 36-37 4.1610218112955515 3.0795625042019594E-4 0.0 1.7646931203853925E-4 7.197179785101208E-4 38-39 4.1684508233141155 3.217969807761598E-4 0.0 1.799294946275302E-4 7.456693479275531E-4 40-41 4.184080468068587 3.2352707207065526E-4 0.0 1.799294946275302E-4 7.871915389954447E-4 42-43 4.204395200048554 3.408279850156101E-4 0.0 1.8857995110000761E-4 8.546650994807685E-4 44-45 4.224469449338585 3.4255807631010556E-4 1.7300912944954827E-6 1.9723040757248502E-4 9.048377470211375E-4 46-47 4.234350000721449 3.4255807631010556E-4 3.4601825889909653E-6 1.9723040757248502E-4 9.446298467945336E-4 48-49 4.2494709986353385 3.494784414880875E-4 3.4601825889909653E-6 1.9723040757248502E-4 0.0010017228595128845 50-51 4.279422339125644 3.529386240770785E-4 3.4601825889909653E-6 2.00690590161476E-4 0.0010553556896422444 52-53 4.305935988213788 3.529386240770785E-4 3.4601825889909653E-6 2.0415077275046697E-4 0.0011020681545936225 54-55 4.339869998864022 3.633191718440514E-4 3.4601825889909653E-6 2.093410466339534E-4 0.0011781921715514239 56-57 4.360755660971172 3.7715990220001523E-4 3.4601825889909653E-6 2.197215944009263E-4 0.001257776371098216 58-59 4.393874798621699 3.8235017608350167E-4 3.4601825889909653E-6 2.2318177698991726E-4 0.001283727740515648 60-61 4.433189393197814 3.910006325559791E-4 3.4601825889909653E-6 2.318322334623947E-4 0.0013477411184119811 62-63 4.483522939228571 3.9273072385047456E-4 3.4601825889909653E-6 2.4394287252386309E-4 0.0013909934007743682 64-65 4.553775026332855 3.97920997733961E-4 3.4601825889909653E-6 2.4567296381835857E-4 0.001394453583363359 66-67 4.60699436464283 3.97920997733961E-4 3.4601825889909653E-6 2.4913314640734953E-4 0.0014100244050138184 68-69 4.634194859974889 4.117617280899249E-4 3.4601825889909653E-6 2.525933289963405E-4 0.0014359757744312508 70-71 4.6616289176317025 4.221422758568978E-4 3.4601825889909653E-6 2.525933289963405E-4 0.001484418330677124 72-73 4.698125193489085 4.2560245844588875E-4 3.4601825889909653E-6 2.525933289963405E-4 0.0015553520737514388 74-75 4.743849776311306 4.2560245844588875E-4 3.4601825889909653E-6 2.6643405935230434E-4 0.00161244508646979 76-77 4.791366733714625 4.2560245844588875E-4 3.4601825889909653E-6 2.6643405935230434E-4 0.0016280159081202492 78-79 4.8433127248318515 4.325228236238707E-4 3.4601825889909653E-6 2.6643405935230434E-4 0.0016505070949486905 80-81 4.908663463298829 4.3598300621286164E-4 3.4601825889909653E-6 2.716243332357908E-4 0.0016781885556606184 82-83 4.996626494984863 4.394431888018526E-4 3.4601825889909653E-6 2.768146071192772E-4 0.0016833788295441045 84-85 5.117253650310972 4.4809364527433E-4 3.4601825889909653E-6 2.889252461807456E-4 0.0017370116596734646 86-87 5.232043477609452 4.498237365688255E-4 3.4601825889909653E-6 3.0622615912570046E-4 0.001799294946275302 88 5.3206985458132845 4.498237365688255E-4 3.4601825889909653E-6 3.114164330091869E-4 0.0018754189632331033 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGG 45015 0.0 71.671425 1 GATAGGA 46220 0.0 70.25729 2 GATCGTA 9610 0.0 63.71828 1 ATAGCGT 8515 0.0 63.422955 8 AATAGCG 8745 0.0 63.252186 7 AGATCGG 186675 0.0 63.148365 1 TAGGAAG 51430 0.0 62.248684 4 ATAGGAA 52590 0.0 60.561935 3 ATCGTAA 10270 0.0 59.532925 2 CGTAAGA 8680 0.0 59.142586 4 CGTAATA 4195 0.0 59.104874 4 TCGTAAG 7330 0.0 58.91426 3 AGCGGAA 76550 0.0 52.68603 2 GATCGGA 413020 0.0 52.15318 1 TCGGAAG 407070 0.0 51.87691 3 ATCGGAA 417410 0.0 51.649635 2 TAGCGTC 9345 0.0 51.299557 9 GCGGAAG 78245 0.0 50.52761 3 GAGCGGA 80685 0.0 49.557957 1 GACGGCA 64090 0.0 49.157646 9 >>END_MODULE