FastQCFastQC Report
Thu 26 May 2016
SRR1660780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28900209
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT28135609.73543132508142TruSeq Adapter, Index 25 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTA18255456.316719024419512TruSeq Adapter, Index 25 (97% over 44bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT646480.22369388401308793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG2544200.072.877341
GATCGGA5590050.051.2784651
ATCGGAA5648850.050.7618372
CGGAAGA5644950.050.664534
TCGGAAG5659350.050.572993
AGAGCAC5935150.048.3329778
GAGCACA5934100.048.3312349
GAAGAGC5966450.048.1156546
GGAAGAG6037500.047.550985
AAGAGCA6049700.047.4146927
TATGCCG5360550.043.96232648-49
CGTATGC5367600.043.8709346-47
CTCGTAT5338300.043.85756744-45
CCGTCTT5325750.043.72524352-53
ATCTCGT5309700.043.6775242-43
TGCCGTC5382500.043.62583550-51
CTTGAAA5130200.043.4240362-63
TGCTTGA5254200.043.37653760-61
ATATCTC5344550.043.35680440-41
ACTGATA5468900.042.9349534-35
Produced by FastQC (version 0.11.5)