FastQCFastQC Report
Thu 26 May 2016
SRR1660779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25410798
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA87550.021.09747946-47
CCGTATC102050.018.2799348-49
TGGTCGC135900.014.468492542-43
GATCTCG143850.013.53768134-35
GTCGCCG155100.012.84355544-45
GTCGTTT54450.012.7669021
GTATCAT156500.012.67582950-51
CCGGGTT65950.012.5922211
CGTATCA86850.012.21998648-49
CGGGGTT105450.012.2112361
TCGTGTA140400.012.04118314-15
CGGGGTA49350.011.9118861
CGGGGGT109600.011.7488581
CGGGGAT84600.011.7464421
CGCGTAT7955.456968E-1211.7370781
CCCGGTT66350.011.6021951
CCCGTAT40200.011.4896361
GCGGAAT39900.011.4590961
ATCATTA182300.011.2379152-53
CCCGTTT61950.011.2212721
Produced by FastQC (version 0.11.5)