FastQCFastQC Report
Thu 26 May 2016
SRR1660779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25410798
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT435360.17132873985303412No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTACC140350.019.4546816
TATGCCG113750.018.9233348-49
CTCGTAT114900.018.67279644-45
CGTATGC119650.018.06909246-47
GTTACCT148650.017.799347
CCAGTTA153000.016.3410764
ATCTCGT130700.016.2355842-43
ACGAGTG138300.015.7842532-33
TACCTTG170750.015.5781589
TCACGAG140400.015.431005530-31
TTACCTT189900.014.9228848
GTCGTTT46150.014.6097311
AGTCACG149400.014.45424328-29
CAGTTAC189700.014.3192725
CCGTCTT158200.013.739902552-53
TGCCGTC164600.013.26282250-51
ATATCTC160650.013.14982840-41
GCATATC77450.012.9246456
CCCGTTT50850.012.7957561
CGCGATA16000.012.63083394
Produced by FastQC (version 0.11.5)