FastQCFastQC Report
Thu 26 May 2016
SRR1660778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26274904
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6431372.447723500721449Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5949922.2644878169678564Illumina Single End PCR Primer 1 (100% over 50bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC797230.30341880602113713Illumina Single End PCR Primer 1 (98% over 50bp)
AAGCAGAAGACGGCATACGAGATTACGTACGGTGACTGGAAAGAGTGTAG693380.26389439900522565Illumina PCR Primer Index 5 (96% over 26bp)
AAGCAGAAGACGGCATACGAGATTACGTACGGAAAGAGTGTAGATCTCGG484480.18438887540749913Illumina PCR Primer Index 5 (96% over 26bp)
AAGCAGAAGACGGCATACGAGATTACGTACGGTGACTGGAGTTCAGACGT438910.16704532964230812Illumina PCR Primer Index 5 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCCG435920.1659073616405982Illumina Single End PCR Primer 1 (98% over 50bp)
CAAGCAGAAGACGGCATACGAGATTACGTACGGTGACTGGAAAGAGTGTA301430.11472163704194696Illumina PCR Primer Index 5 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATTACGTACGGAAAGAGTGTAGATCTCG286680.10910791529438128Illumina PCR Primer Index 5 (96% over 27bp)
AAGCAGAAGACGGCATACGAGATTACGTACGGTGACTGGAAAGAGGGTAG267820.10192996328359563Illumina PCR Primer Index 5 (96% over 26bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGG323500.066.843141
GATAGGA331300.065.565822
AGACGGC703950.058.746178
GACGGCA699400.058.7195479
AGATCGG1292900.057.1046641
AAGACGG731700.056.722337
TAGGAAG382000.056.2892844
ATAGGAA388900.054.7055363
GAAGACG766600.053.678946
AATAGCG46250.047.3185737
ATAGCGT44850.046.637368
AGAAGAC922850.045.6296045
AGCGGAA479400.044.5904162
GATCGTA53750.044.102211
GCAGAAG1045350.043.9648133
GATCGCG339700.043.6303234-35
AAGCAGA1050600.043.483091
AGCAGAA1055800.043.3930932
AGAGCGT2389750.043.2001769
TCGCGGT341300.042.9736836-37
Produced by FastQC (version 0.11.5)