FastQCFastQC Report
Thu 26 May 2016
SRR1660778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26274904
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA12020194.574779797482799TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT11671324.4420029089354625TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509150.19377806289986826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1806050.068.334581
TATGCCG2856200.042.27118348-49
CGTATGC2866200.042.08256546-47
CTCGTAT2850350.041.9653644-45
CCGTCTT2859750.041.6550752-53
ATCTCGT2842300.041.6381842-43
CGTAATC2841500.041.5610538-39
TGCCGTC2892950.041.55149550-51
TACGTAA2892100.041.4684536-37
CGTACGT2904850.041.2853934-35
TGCTTGA2755500.041.03951660-61
ACCGTAC2929250.041.0359232-33
CTTGAAA2663650.040.92475562-63
TAATCTC2887400.040.91567640-41
CTTTCCG135500.040.68292
TCACCGT2971050.040.34864430-31
GATCGGA3097100.040.241252
ATCGGAA3136050.039.678513
TCGGAAG3148950.039.4726874
CGGAAGA3151500.039.463115
Produced by FastQC (version 0.11.5)