##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660778_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26274904 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1087928998713 33.0 33.0 33.0 33.0 33.0 2 32.267435230210545 33.0 33.0 33.0 33.0 33.0 3 32.16888408041377 33.0 33.0 33.0 33.0 33.0 4 32.275152632336926 33.0 33.0 33.0 33.0 33.0 5 32.30772820330761 33.0 33.0 33.0 33.0 33.0 6 35.3163630207745 37.0 37.0 37.0 33.0 37.0 7 35.66074083467631 37.0 37.0 37.0 33.0 37.0 8 35.69559637591825 37.0 37.0 37.0 33.0 37.0 9 35.73848711302618 37.0 37.0 37.0 33.0 37.0 10-11 35.74986275877545 37.0 37.0 37.0 33.0 37.0 12-13 35.7822972445494 37.0 37.0 37.0 33.0 37.0 14-15 35.80034585473652 37.0 37.0 37.0 37.0 37.0 16-17 35.76689610359756 37.0 37.0 37.0 33.0 37.0 18-19 35.6902495818824 37.0 37.0 37.0 33.0 37.0 20-21 35.711722143685094 37.0 37.0 37.0 33.0 37.0 22-23 35.70387258122808 37.0 37.0 37.0 33.0 37.0 24-25 35.73479874560151 37.0 37.0 37.0 33.0 37.0 26-27 35.67882107200087 37.0 37.0 37.0 33.0 37.0 28-29 35.6189780940779 37.0 37.0 37.0 33.0 37.0 30-31 35.552136498767034 37.0 37.0 37.0 33.0 37.0 32-33 35.591959746075574 37.0 37.0 37.0 33.0 37.0 34-35 35.579238101117326 37.0 37.0 37.0 33.0 37.0 36-37 35.533609504339196 37.0 37.0 37.0 33.0 37.0 38-39 35.50453687670942 37.0 37.0 37.0 33.0 37.0 40-41 35.53025442072024 37.0 37.0 37.0 33.0 37.0 42-43 35.402925030668044 37.0 37.0 37.0 33.0 37.0 44-45 35.18890539048211 37.0 37.0 37.0 33.0 37.0 46-47 35.42771416024964 37.0 37.0 37.0 33.0 37.0 48-49 35.46745139392327 37.0 37.0 37.0 33.0 37.0 50-51 35.46096851581265 37.0 37.0 37.0 33.0 37.0 52-53 35.45681986126381 37.0 37.0 37.0 33.0 37.0 54-55 35.433499110025295 37.0 37.0 37.0 33.0 37.0 56-57 35.33100975364172 37.0 37.0 37.0 33.0 37.0 58-59 35.21032533553691 37.0 37.0 37.0 33.0 37.0 60-61 35.31662041467402 37.0 37.0 37.0 33.0 37.0 62-63 35.30732981935918 37.0 37.0 37.0 33.0 37.0 64-65 35.23030961407128 37.0 37.0 37.0 33.0 37.0 66-67 34.74910389777257 37.0 37.0 37.0 27.0 37.0 68-69 34.47092126387979 37.0 37.0 37.0 27.0 37.0 70-71 33.96952550235768 37.0 37.0 37.0 18.0 37.0 72-73 34.369215050224355 37.0 37.0 37.0 27.0 37.0 74-75 34.399451335768916 37.0 37.0 37.0 27.0 37.0 76-77 33.80468609894826 37.0 37.0 37.0 20.5 37.0 78-79 33.712393468687836 37.0 37.0 37.0 18.0 37.0 80-81 33.91405270215259 37.0 37.0 37.0 22.0 37.0 82-83 33.81827056342432 37.0 37.0 37.0 22.0 37.0 84-85 33.80649931204316 37.0 37.0 37.0 22.0 37.0 86-87 33.80671143460695 37.0 37.0 37.0 22.0 37.0 88-89 33.86087043362747 37.0 37.0 37.0 22.0 37.0 90-91 33.83220724612353 37.0 37.0 37.0 22.0 37.0 92-93 33.755675320450266 37.0 37.0 37.0 18.0 37.0 94-95 33.73790878550879 37.0 37.0 37.0 22.0 37.0 96-97 33.721776300305415 37.0 37.0 37.0 22.0 37.0 98-99 33.70642880369801 37.0 37.0 37.0 22.0 37.0 100 33.61645991170891 37.0 37.0 37.0 14.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02989107606542518 1101 2 -0.04973301084645243 1101 3 -0.07550710934399518 1101 4 0.061863458930581317 1101 5 0.06185581048058708 1101 6 0.2620102196955898 1101 7 0.258225743586614 1101 8 0.29955741454389795 1101 9 0.3013827601046657 1101 10-11 0.28156222481148774 1101 12-13 0.25917193990301257 1101 14-15 0.27785091210073887 1101 16-17 0.33710343041641266 1101 18-19 0.392035184116061 1101 20-21 0.3794190800495727 1101 22-23 0.28588137691824045 1101 24-25 0.32672662679437536 1101 26-27 0.3449674327547001 1101 28-29 0.29115483188893165 1101 30-31 0.39811298260508465 1101 32-33 0.28849054566756394 1101 34-35 0.3522072204377551 1101 36-37 0.3621201007739998 1101 38-39 0.3139438550513134 1101 40-41 0.37893156487083957 1101 42-43 0.36543359912785434 1101 44-45 0.3821963362587937 1101 46-47 0.375856538805742 1101 48-49 0.31126385537742607 1101 50-51 0.3425682403971848 1101 52-53 0.2751546996710985 1101 54-55 0.3389199537346457 1101 56-57 0.35138493677642657 1101 58-59 0.36885955876024923 1101 60-61 0.2353546614805424 1101 62-63 0.1966243967498471 1101 64-65 0.23574731682629846 1101 66-67 0.28442839216938154 1101 68-69 0.23054037173039887 1101 70-71 0.31742183771225996 1101 72-73 0.28864157341863717 1101 74-75 0.32518886646566614 1101 76-77 0.3009389138662897 1101 78-79 0.4167721776482267 1101 80-81 0.32408479094599585 1101 82-83 0.34385611433285135 1101 84-85 0.38552116123310753 1101 86-87 0.4105410598092405 1101 88-89 0.3345099989442346 1101 90-91 0.3283994381543991 1101 92-93 0.3918980862020973 1101 94-95 0.29779594305987445 1101 96-97 0.31649991338318273 1101 98-99 0.4261841859770499 1101 100 0.3586792300223607 1102 1 -0.01584889274198531 1102 2 0.02021049277289677 1102 3 0.1623760743650564 1102 4 0.07657503389807374 1102 5 0.07715626475710025 1102 6 0.3552866274389004 1102 7 0.2408332582376289 1102 8 0.25918729678683405 1102 9 0.2979388080243339 1102 10-11 0.3280757337065481 1102 12-13 0.2886645908158698 1102 14-15 0.25649315094519665 1102 16-17 0.33958030194528277 1102 18-19 0.4332727583845255 1102 20-21 0.3598440384727084 1102 22-23 0.2702611780974351 1102 24-25 0.23088721689411784 1102 26-27 0.16349760790626533 1102 28-29 0.31717780218658476 1102 30-31 0.39742571857592424 1102 32-33 0.3562449929524121 1102 34-35 0.36058306307730703 1102 36-37 0.35405675434324735 1102 38-39 0.3202277994026801 1102 40-41 0.2944395327327243 1102 42-43 0.2873658748626511 1102 44-45 0.3240417876835693 1102 46-47 0.3613230676027044 1102 48-49 0.3032254892526538 1102 50-51 0.33388944094006945 1102 52-53 0.3200544601442843 1102 54-55 0.2908540006009588 1102 56-57 0.383024207854298 1102 58-59 0.3986474502486672 1102 60-61 0.2994243365870872 1102 62-63 0.27981903584971946 1102 64-65 0.335263571894167 1102 66-67 0.2537070398443717 1102 68-69 0.26941714689919394 1102 70-71 0.27271294726351414 1102 72-73 0.2561909208412345 1102 74-75 0.3611478672834423 1102 76-77 0.34292352195104314 1102 78-79 0.34857728701698676 1102 80-81 0.34636851800325985 1102 82-83 0.3534861213508549 1102 84-85 0.4055170594213422 1102 86-87 0.37498525107832137 1102 88-89 0.21724505942923855 1102 90-91 0.29802610077902614 1102 92-93 0.22088956962076622 1102 94-95 0.284798739597953 1102 96-97 0.2991687680481263 1102 98-99 0.427626743847739 1102 100 0.3440281245940824 1103 1 -0.003399219498568584 1103 2 0.07415146672482109 1103 3 -0.003914941441870212 1103 4 0.07455922186281327 1103 5 0.06859801720653991 1103 6 0.25532306463401966 1103 7 0.22580688393577475 1103 8 0.2505637130335856 1103 9 0.3225721267762722 1103 10-11 0.30091190334405127 1103 12-13 0.22335959615168122 1103 14-15 0.16141114608889495 1103 16-17 0.17609890648939341 1103 18-19 0.3334416707844028 1103 20-21 0.2560750990573766 1103 22-23 0.148288298082619 1103 24-25 0.02605520580001297 1103 26-27 0.13759460430568993 1103 28-29 0.26010977332779817 1103 30-31 0.34266913556864154 1103 32-33 0.22709530483544427 1103 34-35 0.2427025102747109 1103 36-37 0.3275083022114913 1103 38-39 0.2700174396125803 1103 40-41 0.2758082528310126 1103 42-43 0.19932991019057766 1103 44-45 0.12694175771004268 1103 46-47 0.11795723168565786 1103 48-49 0.09116115900500432 1103 50-51 0.1377788830619906 1103 52-53 0.17728730124581915 1103 54-55 0.15767854206865906 1103 56-57 0.21473429135187416 1103 58-59 0.3275994860989542 1103 60-61 0.1062547210912328 1103 62-63 0.18675148618863346 1103 64-65 0.2628688828942316 1103 66-67 0.18319602828955084 1103 68-69 0.22324458789194068 1103 70-71 0.1728179154156635 1103 72-73 0.16303644211814117 1103 74-75 0.14883666939936546 1103 76-77 0.21840500633473425 1103 78-79 0.28331932877225086 1103 80-81 0.2549416499206316 1103 82-83 0.2639888013178364 1103 84-85 0.3310807264551272 1103 86-87 0.33185828109335347 1103 88-89 0.1563264084008651 1103 90-91 0.15844472647130203 1103 92-93 0.2715796994847466 1103 94-95 0.09982064840716731 1103 96-97 0.22560630468596798 1103 98-99 0.30780802158328413 1103 100 0.12951930161945313 1104 1 0.002329329249690204 1104 2 0.0373581579333333 1104 3 -0.029675530054724675 1104 4 0.10951292639304455 1104 5 0.05079883584141953 1104 6 0.30561528343363165 1104 7 0.23555483159503154 1104 8 0.25630381892257503 1104 9 0.31246142505193575 1104 10-11 0.322345328557212 1104 12-13 0.30527769002789285 1104 14-15 0.2559427703802868 1104 16-17 0.29337219317971375 1104 18-19 0.32447729365183164 1104 20-21 0.3052153509556632 1104 22-23 0.2365838424578186 1104 24-25 0.2582418866814038 1104 26-27 0.2688221331613079 1104 28-29 0.3093727294693167 1104 30-31 0.3651436850818044 1104 32-33 0.23567001361357143 1104 34-35 0.2188568920511642 1104 36-37 0.2745705854893785 1104 38-39 0.25102422290892434 1104 40-41 0.263498428279199 1104 42-43 0.2851017738008821 1104 44-45 0.2727467703478723 1104 46-47 0.26802787484744073 1104 48-49 0.27104790019375713 1104 50-51 0.2259220864693674 1104 52-53 0.18029897226721658 1104 54-55 0.20875116958816875 1104 56-57 0.338799262771758 1104 58-59 0.38004985910817624 1104 60-61 0.28127523445986924 1104 62-63 0.24905599295405523 1104 64-65 0.1904040657393793 1104 66-67 0.18099139385164875 1104 68-69 0.16880443453191418 1104 70-71 0.18878498269242527 1104 72-73 0.23200043421661576 1104 74-75 0.2853917245088482 1104 76-77 0.2394836111985299 1104 78-79 0.34294902207677325 1104 80-81 0.2866115038665171 1104 82-83 0.28046257107438777 1104 84-85 0.3618158201712447 1104 86-87 0.37752162587737814 1104 88-89 0.2261649320638952 1104 90-91 0.33549331642839064 1104 92-93 0.3922181705544432 1104 94-95 0.306541497819417 1104 96-97 0.3220641241978939 1104 98-99 0.3931148660056749 1104 100 0.2994370147774248 1105 1 0.017502482343900283 1105 2 0.060323328561651124 1105 3 0.17006788588288657 1105 4 0.06971541211269994 1105 5 0.0352260521629546 1105 6 0.2572657834733718 1105 7 0.24268837823419886 1105 8 0.2360989485378937 1105 9 0.31404723055047157 1105 10-11 0.30396064523527144 1105 12-13 0.29254361284961306 1105 14-15 0.25231260351232976 1105 16-17 0.31430629120915654 1105 18-19 0.38956204450084186 1105 20-21 0.3716474665816989 1105 22-23 0.3267197250521292 1105 24-25 0.2617492143443698 1105 26-27 0.3353834375917444 1105 28-29 0.3635714106615211 1105 30-31 0.3677387166303774 1105 32-33 0.33222943904021207 1105 34-35 0.2846502172160328 1105 36-37 0.336227141550296 1105 38-39 0.3507139268937891 1105 40-41 0.29318029390442746 1105 42-43 0.33653144075706365 1105 44-45 0.3070641881941967 1105 46-47 0.3285919755114648 1105 48-49 0.3054779764470581 1105 50-51 0.2986910242849632 1105 52-53 0.29314044024271624 1105 54-55 0.32156288488361895 1105 56-57 0.35095930536181896 1105 58-59 0.4161061763899596 1105 60-61 0.3002593555168147 1105 62-63 0.2974084835152624 1105 64-65 0.30081130667030465 1105 66-67 0.33166811446818656 1105 68-69 0.33445630440800045 1105 70-71 0.3610870969281592 1105 72-73 0.3853162658585134 1105 74-75 0.37524044563874526 1105 76-77 0.3737019354012858 1105 78-79 0.38732557314062177 1105 80-81 0.3794311278631355 1105 82-83 0.38795674551537473 1105 84-85 0.4237340741678892 1105 86-87 0.3807749023975404 1105 88-89 0.38896453121915897 1105 90-91 0.39194273080834563 1105 92-93 0.44170041489667256 1105 94-95 0.3797352719375269 1105 96-97 0.34067677435356813 1105 98-99 0.4125572227058143 1105 100 0.36710075051134083 1106 1 0.02738794779676823 1106 2 0.037722180246873904 1106 3 0.13003008732605537 1106 4 0.04735837191229564 1106 5 -0.00431603593488461 1106 6 0.15940990885674466 1106 7 0.2444488903469093 1106 8 0.28102054025102063 1106 9 0.296361084167863 1106 10-11 0.31468685299039123 1106 12-13 0.27984247257028017 1106 14-15 0.26494918321409244 1106 16-17 0.29696174153532695 1106 18-19 0.39100314534356784 1106 20-21 0.3148575849863491 1106 22-23 0.3078523329296914 1106 24-25 0.246345958852892 1106 26-27 0.28789410126411497 1106 28-29 0.3363137839091266 1106 30-31 0.3576073387974148 1106 32-33 0.3352396297793945 1106 34-35 0.275908913314872 1106 36-37 0.34857845445561253 1106 38-39 0.3212151985884759 1106 40-41 0.28683149723410395 1106 42-43 0.27629166518345727 1106 44-45 0.32392334419374436 1106 46-47 0.34096164305466203 1106 48-49 0.2738154033055977 1106 50-51 0.21100862279477894 1106 52-53 0.21743346653595808 1106 54-55 0.2104620415359406 1106 56-57 0.3081177394628014 1106 58-59 0.3880623537359966 1106 60-61 0.28692243283504126 1106 62-63 0.26690471292258877 1106 64-65 0.2839373255649349 1106 66-67 0.21619920338725507 1106 68-69 0.23532934567771235 1106 70-71 0.15410378037277894 1106 72-73 0.25682522926585705 1106 74-75 0.26807617803535777 1106 76-77 0.21131062510492882 1106 78-79 0.2935485956858699 1106 80-81 0.29736044199959366 1106 82-83 0.2985504487991193 1106 84-85 0.3296446353810012 1106 86-87 0.26599502466364555 1106 88-89 0.30877618925604366 1106 90-91 0.33948727659593914 1106 92-93 0.34229937380897724 1106 94-95 0.335556612578813 1106 96-97 0.3188628931185349 1106 98-99 0.29742576019621936 1106 100 0.24309304243108443 1107 1 -0.028519656190454157 1107 2 0.01993196765844374 1107 3 0.09035775634899323 1107 4 0.043975805000243895 1107 5 0.0010395418678186275 1107 6 0.3006685854739928 1107 7 0.22672416221617908 1107 8 0.21277974046366666 1107 9 0.26229108680768576 1107 10-11 0.2789487809347193 1107 12-13 0.2612306881427173 1107 14-15 0.2467283697455187 1107 16-17 0.2609604578061422 1107 18-19 0.3064894064379686 1107 20-21 0.30459523306659975 1107 22-23 0.17898519797966372 1107 24-25 0.21427578246876777 1107 26-27 0.27579863894841594 1107 28-29 0.31154394880594793 1107 30-31 0.3698040599323775 1107 32-33 0.26148932541172343 1107 34-35 0.2942905158966269 1107 36-37 0.35080168769420794 1107 38-39 0.2913674099957291 1107 40-41 0.2624853866625756 1107 42-43 0.2745908269060564 1107 44-45 0.27303372166100814 1107 46-47 0.3011240334862393 1107 48-49 0.2790675209233271 1107 50-51 0.24320147377000012 1107 52-53 0.22957570069984712 1107 54-55 0.23155421261571973 1107 56-57 0.3040110266588911 1107 58-59 0.38372391299220254 1107 60-61 0.2418451524736014 1107 62-63 0.2551384351890462 1107 64-65 0.2180504823715239 1107 66-67 0.26513791943695963 1107 68-69 0.2634801702365763 1107 70-71 0.25481115317909087 1107 72-73 0.23456296991689385 1107 74-75 0.29017765294211273 1107 76-77 0.19079607597396375 1107 78-79 0.20891949026960077 1107 80-81 0.22455200052824864 1107 82-83 0.18434600165655013 1107 84-85 0.2693977031639605 1107 86-87 0.208495134618488 1107 88-89 0.23562956889865916 1107 90-91 0.28296571644813895 1107 92-93 0.302967557207495 1107 94-95 0.2390399214329264 1107 96-97 0.2160622964364478 1107 98-99 0.2839405461431568 1107 100 0.16974146151311942 1108 1 -0.01980888446923501 1108 2 -0.023831535383287417 1108 3 0.09300533280747203 1108 4 0.014408145752888402 1108 5 -0.06797201032036781 1108 6 0.027096272034242475 1108 7 0.2031623351877272 1108 8 0.23077686811930676 1108 9 0.26011352288770695 1108 10-11 0.2530224540275583 1108 12-13 0.24861056779428736 1108 14-15 0.22403318220005985 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-0.5885155802038966 2316 82-83 -0.5893887220894385 2316 84-85 -0.6547573936422353 2316 86-87 -0.6018677773747001 2316 88-89 -0.5776291030851297 2316 90-91 -0.6444039575191525 2316 92-93 -0.5826503411961212 2316 94-95 -0.6268441018172055 2316 96-97 -0.7675606057478319 2316 98-99 -0.7992422104632482 2316 100 -0.7742807105870781 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 1.0 13 2.0 14 817.0 15 76259.0 16 272278.0 17 117690.0 18 24268.0 19 10624.0 20 11673.0 21 18605.0 22 31394.0 23 50618.0 24 77908.0 25 116042.0 26 164082.0 27 226975.0 28 306117.0 29 419626.0 30 641699.0 31 1131042.0 32 998900.0 33 1264708.0 34 1943913.0 35 2642017.0 36 1.5727642E7 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.905442525285665 17.273177331375475 14.42698401704494 33.39439612629391 2 25.52822267209806 22.696124788886003 29.359243329680673 22.416409209335264 3 24.0520003422277 23.381699130090066 29.890956024044847 22.675344503637387 4 25.516538519037024 23.898751447388733 25.882126914716796 24.702583118857447 5 31.36165216816777 25.277961053635057 22.278300236606004 21.08208654159117 6 31.712096835824788 30.934057837090478 19.09770631321812 18.256139013866616 7 25.365946151506396 21.883870631839418 32.359341826710384 20.3908413899438 8 21.648577669398904 30.258219021466264 23.96697243879559 24.126230870339242 9 26.036563254427115 23.57668747333958 27.3851428724535 23.001606399779806 10-11 28.685347432668067 30.752983911948835 19.48769251449977 21.073976140883328 12-13 24.973604090047292 24.11851247867547 23.60128508937654 27.306598341900695 14-15 22.328897947638556 27.731583719582765 23.197926812596535 26.741591520182144 16-17 24.905263212379385 25.2065297745712 23.207232270001825 26.680974743047585 18-19 24.895550522277837 22.77805467909607 27.824251993461136 24.502142805164958 20-21 24.924526841277896 22.78959801337428 27.96931627228781 24.31655887306001 22-23 24.89904054454395 30.298991767962313 22.990909881155037 21.811057806338702 24-25 22.316863650576995 25.27457379102127 25.436279424655556 26.972283133746178 26-27 22.479254348560133 22.736155382337458 25.188967388805683 29.59562288029673 28-29 24.896183709330028 27.560498473476503 22.94742832213034 24.59588949506313 30-31 24.698461315025167 22.913573727995352 27.899464447139373 24.48850050984011 32-33 22.172497756794847 27.51930701630727 22.951341706139058 27.356853520758822 34-35 25.098282040512288 22.552853880426987 22.821315497700237 29.527548581360485 36-37 24.59372732984019 25.10111835617433 27.993682793043995 22.311471520941488 38-39 24.76575602005587 25.205748154755213 23.161991682442654 26.866504142746262 40-41 24.815587306512057 25.236220960919002 27.82037737808035 22.12781435448859 42-43 22.08742381302381 30.072287426994777 25.425938023124182 22.414350736857234 44-45 22.278368246550087 22.779933631944736 27.989276585305117 26.95242153620006 46-47 24.650023190807318 23.131279836648723 25.327537683563285 26.891159288980678 48-49 24.44882296670422 25.559737874040568 27.85697578352531 22.134463375729904 50-51 24.58189557621142 25.539919509768755 27.718726095857654 22.15945881816217 52-53 24.613439430245695 23.21347293060763 22.757659439857356 29.415428199289323 54-55 26.835015710528552 23.347865441413855 25.12713477505014 24.689984073007455 56-57 22.009319885189964 23.401943826753065 27.735678641510482 26.853057646546493 58-59 22.10571772413931 23.34679364283869 29.940304062391704 24.6071845706303 60-61 24.26748572473023 23.550877791245426 27.465962521832864 24.715673962191477 62-63 24.315877000173455 23.649667109138324 25.149740230664396 26.884715660023815 64-65 24.506479826326693 23.48040424305707 29.80916123798028 22.203954692635957 66-67 24.83380275986414 30.46400188293311 22.609020921216803 22.093174435985947 68-69 21.993395491709844 32.894196788070296 22.742933802600227 22.369473917619636 70-71 22.49898740345888 32.30546252540245 22.505179828087332 22.690370243051333 72-73 22.253368718049764 32.77133510647671 22.5124607656726 22.462835409800924 74-75 22.5282324414141 32.62165732959513 22.634097792942445 22.216012436048327 76-77 24.130956840375074 29.80040613605398 22.524023220867328 23.54461380270362 78-79 22.46598133987702 29.02803806471132 22.823621581106625 25.68235901430504 80-81 22.360599244895273 28.837106329190092 22.850686004811447 25.951608421103188 82-83 25.112896815915576 25.60535771577614 24.158741571572854 25.123003896735437 84-85 22.96786596799162 24.85795639166884 26.459819667211164 25.714357973128372 86-87 25.217229582527985 26.22037636113086 25.199590782893594 23.362803273447565 88-89 23.104852718895245 29.60213677725107 23.109962522776215 24.183047981077475 90-91 22.428162503460847 25.141290722542443 25.8931418624603 26.537404911536406 92-93 22.65816312063621 25.69142693590122 23.584641245471477 28.065768697991096 94-95 25.297333445914333 26.75573886695976 24.398272532456524 23.54865515466938 96-97 22.954647299869233 25.838199124724643 25.34376628733704 25.863387288069084 98-99 22.57422261790965 26.83526787580749 23.119672080640207 27.470837425642653 100 24.810596581804035 24.74176240089482 23.08959362752137 27.35804738977977 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45726.0 1 27573.5 2 6812.0 3 3694.5 4 3161.5 5 2930.5 6 2639.5 7 3307.0 8 3461.5 9 2786.5 10 2917.5 11 3487.5 12 3925.5 13 4740.5 14 6273.5 15 6767.0 16 6873.5 17 7842.0 18 9337.5 19 11563.5 20 15091.0 21 19796.5 22 25140.5 23 31962.5 24 40905.0 25 51864.5 26 65621.0 27 83504.0 28 103695.0 29 129085.5 30 160076.5 31 198893.5 32 239551.0 33 276423.5 34 313533.5 35 363816.0 36 425919.0 37 484689.0 38 536762.5 39 580752.0 40 620395.0 41 652379.5 42 691021.5 43 736651.0 44 788057.0 45 853760.0 46 925770.5 47 1057547.0 48 1232116.0 49 1263124.5 50 1141648.5 51 1051664.5 52 1012468.0 53 969898.5 54 925161.0 55 883942.0 56 846756.0 57 802655.0 58 751990.0 59 687287.5 60 610380.0 61 536274.0 62 463341.0 63 388275.0 64 320386.0 65 264635.0 66 220383.5 67 183623.5 68 151806.5 69 127439.0 70 108616.0 71 94365.0 72 84478.5 73 76421.5 74 68787.5 75 62109.5 76 56063.5 77 51223.5 78 45150.0 79 38482.5 80 34079.0 81 29157.5 82 22689.5 83 17807.0 84 13346.0 85 8833.5 86 5692.5 87 3354.5 88 1809.0 89 807.5 90 343.0 91 145.0 92 87.0 93 60.0 94 34.0 95 30.0 96 27.5 97 16.0 98 7.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3211353312651494 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.0932521770583823E-5 30-31 0.0 32-33 0.0 34-35 4.567095659036471E-5 36-37 3.8059130491970593E-6 38-39 9.514782622992648E-6 40-41 5.328278268875883E-4 42-43 2.7402573954218824E-4 44-45 2.4548139167321033E-4 46-47 0.001843964872335975 48-49 0.0045651927025118725 50-51 1.4652765239408678E-4 52-53 0.00213511722059955 54-55 0.0012426306105628399 56-57 0.0020780285248615943 58-59 1.9029565245985297E-6 60-61 2.4738434819780885E-5 62-63 5.765958269533544E-4 64-65 0.004589931137331653 66-67 0.003813524875295453 68-69 0.0027783165259138527 70-71 0.0032959207006046528 72-73 0.00394673183201735 74-75 0.0036650942663767677 76-77 0.0036156173967372062 78-79 0.0038630017449350147 80-81 0.0010294994798078043 82-83 0.0047364587897257395 84-85 0.0011969596539724752 86-87 0.012464365236120368 88-89 0.0071551165324904705 90-91 0.002361569047026775 92-93 0.004411053224019391 94-95 0.0018591885245327633 96-97 0.0025042907863716646 98-99 0.001969560002959478 100 6.355874792159089E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6274904E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.116964644807915 #Duplication Level Percentage of deduplicated Percentage of total 1 68.3836997721948 30.85264964903272 2 17.40940219481675 15.70918766621577 3 6.209236549960756 8.404257176874856 4 2.7564625284311766 4.974528897598689 5 1.4774821417394224 3.3329754776096303 6 0.8651278476801719 2.341916551021505 7 0.5970295422003682 1.8855312523151906 8 0.4154622821717405 1.4995517676794903 9 0.30634956436228555 1.2439406217856992 >10 1.448344807421299 11.30298881446351 >50 0.08113414097575126 2.5118369177927082 >100 0.044995584939732707 3.804184538741883 >500 0.0036672141816900764 1.118568061990709 >1k 0.0014448775602335406 1.1543934367037256 >5k 8.471124412871072E-5 0.2739739198836083 >10k+ 7.624011971556239E-5 9.589515250290269 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA 1202019 4.574779797482799 TruSeq Adapter, Index 22 (97% over 40bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT 1167132 4.4420029089354625 TruSeq Adapter, Index 22 (97% over 40bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50915 0.19377806289986826 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 4.921985633134949 3.805913049197059E-6 0.0 0.0 3.805913049197059E-5 2 4.940760202206638 2.6641391344379413E-5 0.0 1.5223652196788236E-5 4.1865043541167647E-5 3 4.941228329511689 2.6641391344379413E-5 0.0 1.5223652196788236E-5 8.373008708233529E-5 4 4.9428192011662535 2.6641391344379413E-5 0.0 1.9029565245985296E-5 1.2178921757430588E-4 5 4.944569921168884 3.805913049197059E-5 0.0 1.9029565245985296E-5 1.9790747855824705E-4 6 4.946442430389089 4.9476869639561764E-5 0.0 1.9029565245985296E-5 2.0551930465664118E-4 7 4.948459564305163 5.3282782688758825E-5 0.0 3.044730439357647E-5 2.8163756564058233E-4 8 4.956851602578643 7.611826098394118E-5 0.0 3.425321744277353E-5 2.892493917389765E-4 9 4.9623206996303395 8.753600013153235E-5 0.0 3.425321744277353E-5 3.0827895698496175E-4 10-11 4.966343549723341 1.1037147842671471E-4 0.0 3.805913049197059E-5 3.3492034832934117E-4 12-13 4.972807893037402 1.5604243501707941E-4 0.0 4.1865043541167647E-5 4.5290365285445E-4 14-15 4.978733699655002 1.8648973941065588E-4 0.0 4.9476869639561764E-5 5.023805224940118E-4 16-17 4.985454942099883 1.8648973941065588E-4 0.0 5.3282782688758825E-5 5.556633051827706E-4 18-19 4.991447352195845 1.8648973941065588E-4 0.0 5.708869573795588E-5 6.260726965929162E-4 20-21 5.000237108382965 1.9029565245985296E-4 0.0 5.708869573795588E-5 6.660347836094853E-4 22-23 5.006402687522664 1.9790747855824705E-4 0.0 6.470052183635E-5 6.926761749538647E-4 24-25 5.012724309097381 2.0551930465664118E-4 0.0 7.611826098394118E-5 7.706973924624045E-4 26-27 5.018733845802062 2.0932521770583823E-4 0.0 7.611826098394118E-5 8.905836535121117E-4 28-29 5.024402753288841 2.26451826427225E-4 0.0 7.802121750853971E-5 9.248368709548853E-4 30-31 5.030537885124147 2.340636525256191E-4 0.0 7.992417403313823E-5 9.362546101024764E-4 32-33 5.036463691741748 2.4167547862401326E-4 0.0 7.992417403313823E-5 9.552841753484617E-4 34-35 5.042054578011017 2.473843481978088E-4 0.0 8.373008708233529E-5 9.781196536436442E-4 36-37 5.048071726541798 2.5499617429620297E-4 0.0 8.943895665613089E-5 0.0010523349581029867 38-39 5.054933787769501 2.6070504386999853E-4 0.0 9.324486970532794E-5 0.0010980059146933514 40-41 5.063253513695045 2.645109569191956E-4 0.0 9.514782622992648E-5 0.0011417739147591176 42-43 5.073776863276075 2.683168699683927E-4 0.0 9.514782622992648E-5 0.0012007655670216721 44-45 5.082380129723785 2.7021982649299116E-4 0.0 9.514782622992648E-5 0.0012692720019072193 46-47 5.089407748169128 2.721227830175897E-4 0.0 9.895373927912353E-5 0.001339681393317365 48-49 5.127898849792182 2.759286960667868E-4 0.0 1.0275965232832059E-4 0.0014291203499734958 50-51 5.169899003246596 2.854434786897794E-4 0.0 1.0275965232832059E-4 0.0015090445240066338 52-53 5.183168319092622 2.854434786897794E-4 0.0 1.0275965232832059E-4 0.001537588871875612 54-55 5.194654564675098 2.968612178373706E-4 0.0 1.0846852190211619E-4 0.0017088549590894794 56-57 5.2049571712992755 3.1208487003415883E-4 0.0 1.1037147842671471E-4 0.0018401589592867778 58-59 5.217615638100904 3.1779373960795444E-4 0.0 1.1037147842671471E-4 0.0018725092202049529 60-61 5.235063846474948 3.196966961325529E-4 0.0 1.1227443495131324E-4 0.0019257920028937117 62-63 5.2604074214695515 3.196966961325529E-4 0.0 1.1417739147591176E-4 0.0019600452203364853 64-65 5.287593058379966 3.2350260918175E-4 0.0 1.1798330452510882E-4 0.0019809777421070695 66-67 5.355069993785705 3.3111443528014415E-4 0.0 1.1988626104970736E-4 0.0020114250465006456 68-69 5.378554380255776 3.4633808747693234E-4 0.0 1.2559513062350295E-4 0.002058998959615609 70-71 5.41014726447716 3.539499135753265E-4 0.0 1.294010436727E-4 0.002116087655353565 72-73 5.445184500008068 3.539499135753265E-4 0.0 1.3701286977109412E-4 0.0022188473076818855 74-75 5.490117109466889 3.539499135753265E-4 0.0 1.3891582629569266E-4 0.002317801046961009 76-77 5.536813759624012 3.5965878314912205E-4 0.0 1.4081878282029117E-4 0.002346345394829987 78-79 5.588838688050012 3.615617396737206E-4 0.0 1.4462469586948824E-4 0.002372986786174366 80-81 5.651632447448714 3.6536765272291767E-4 0.0 1.484306089186853E-4 0.0023958222644695484 82-83 5.734835415573736 3.691735657721147E-4 0.0 1.541394784924809E-4 0.002416754786240132 84-85 5.844070828955265 3.691735657721147E-4 0.0 1.61751304590875E-4 0.0024890671341748763 86-87 5.957239653473139 3.691735657721147E-4 0.0 1.8648973941065588E-4 0.002584214960404803 88 6.047995456044292 3.691735657721147E-4 0.0 1.8648973941065588E-4 0.0026793627866347294 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 180605 0.0 68.33458 1 TATGCCG 285620 0.0 42.271183 48-49 CGTATGC 286620 0.0 42.082565 46-47 CTCGTAT 285035 0.0 41.96536 44-45 CCGTCTT 285975 0.0 41.65507 52-53 ATCTCGT 284230 0.0 41.63818 42-43 CGTAATC 284150 0.0 41.56105 38-39 TGCCGTC 289295 0.0 41.551495 50-51 TACGTAA 289210 0.0 41.46845 36-37 CGTACGT 290485 0.0 41.28539 34-35 TGCTTGA 275550 0.0 41.039516 60-61 ACCGTAC 292925 0.0 41.03592 32-33 CTTGAAA 266365 0.0 40.924755 62-63 TAATCTC 288740 0.0 40.915676 40-41 CTTTCCG 13550 0.0 40.6829 2 TCACCGT 297105 0.0 40.348644 30-31 GATCGGA 309710 0.0 40.24125 2 ATCGGAA 313605 0.0 39.67851 3 TCGGAAG 314895 0.0 39.472687 4 CGGAAGA 315150 0.0 39.46311 5 >>END_MODULE