FastQCFastQC Report
Thu 26 May 2016
SRR1660777_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660777_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22326913
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10585054.7409375402681055Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3850371.7245420358828825Illumina Single End PCR Primer 1 (100% over 50bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC529110.23698305269519346Illumina Single End PCR Primer 1 (98% over 50bp)
AAGCAGAAGACGGCATACGAGATTCCGAAACGTGACTGGAAAGAGTGTAG351030.15722280997825358RNA PCR Primer, Index 40 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTCCGAAACGTGACTGGAAAGAGTGTA347820.1557850832311659RNA PCR Primer, Index 40 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCCG254180.11384466809182263Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG51650.062.5476767
ATAGCGT50000.061.604878
AGATAGG221950.060.054691
GATCGTA68250.059.8606341
GATAGGA231900.059.1479872
GATCGGA2582000.058.662361
TCGGAAG2537150.058.0855673
ATCGGAA2622550.057.661842
AGCGGAA601650.056.6345522
CGTAAGA64350.056.1589624
ATCGTAA73150.055.7240642
GAGCGTC2451550.055.1921469
TCGTAAG53900.055.167593
AGAGCGT2697450.055.0328458
GCGGAAG604400.054.904443
GAGCGGA613550.054.3343471
CGTAATA26250.054.3287054
CGGAAGA3456600.052.8819734
TAGGAAG281150.048.154084
AAGAGCG3610850.047.5783737
Produced by FastQC (version 0.11.5)