FastQCFastQC Report
Thu 26 May 2016
SRR1660777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22326913
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT19618118.786754353367167TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA7078403.170344238811698TruSeq Adapter, Index 21 (97% over 40bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA757110.33910196183413266TruSeq Adapter, Index 21 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344690.15438318768026732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3790100.057.79191
ATCGGAA3827200.057.17182
TCGGAAG3803800.057.0132453
CGGAAGA3797650.056.963264
AGATCGG1359250.056.3225441
GAAGAGC4127600.052.5350046
GGAAGAG4129700.052.5264975
GAGCACA4167500.051.6700489
AGAGCAC4176350.051.634828
AAGAGCA4287650.050.445737
GGATCGG189000.045.6276131
CGGAATC3344900.042.9002338-39
ACGTTTC3437300.042.83954632-33
CTCGTAT3371850.042.76582744-45
GTTTCGG3432650.042.65006334-35
CGTATGC3405000.042.6443646-47
ATCTCGT3350050.042.6089742-43
TATGCCG3424750.042.56176448-49
TTCGGAA3438700.042.3894436-37
CCGTCTT3417600.042.15064252-53
Produced by FastQC (version 0.11.5)