FastQCFastQC Report
Thu 26 May 2016
SRR1660776_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660776_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19185783
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4807642.5058346589242673Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1629240.8491912996201407Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAG245510.12796454541365343Illumina Single End PCR Primer 1 (100% over 30bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC203840.10624533801930315Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAA262350.056.506972
GATCGGA1221900.051.815441
GAGCGGA280800.051.796221
ATCGGAA1246850.050.693742
GCGGAAG285050.050.6863173
GAGCGTC1175500.050.1076479
TCGGAAG1231950.050.0362363
AGAGCGT1305300.048.9478388
CGGAAGA1587250.048.4516034
AATAGCG33450.046.5265587
AAGAGCG1690050.042.810337
ATAGCGT35250.041.6752178
GATCGCG128150.040.5222834-35
CGTAATA18450.039.9431884
TCGCGGT130450.039.30742336-37
CGCCGTA1196950.039.04044346-47
CCGTATC1228550.038.97625748-49
TGGTCGC1295400.038.40702442-43
GGAAGAG2028500.038.370855
AGATAGG122750.038.343231
Produced by FastQC (version 0.11.5)