FastQCFastQC Report
Thu 26 May 2016
SRR1660776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19185783
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT7985874.162389410950807TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA2697031.405744034528067TruSeq Adapter, Index 19 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCACACGTCTGAACTCCAG250840.13074264417563775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1682500.054.5878331
ATCGGAA1708400.053.7213752
CGGAAGA1719900.053.1162454
TCGGAAG1722800.053.0895733
AGAGCAC1833450.049.701038
GAGCACA1830050.049.644439
AAGAGCA1928300.047.339187
GAAGAGC1933600.047.301786
AGATCGG680450.045.9918331
GGAAGAG2019250.045.4676175
AACGATC1410000.040.689938-39
ATCTCGT1421250.040.14169342-43
CTCGTAT1454600.040.12791444-45
CGATCTC1424200.039.8540940-41
GAAACGA1513200.039.2192636-37
ACGTGAA1558700.038.87300532-33
GTGAAAC1571850.038.281934-35
CGTATGC1594350.037.43321246-47
TCACGTG1611450.037.4269930-31
TATGCCG1602800.037.3877148-49
Produced by FastQC (version 0.11.5)