FastQCFastQC Report
Thu 26 May 2016
SRR1660775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25290588
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12590994.978527980448695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490080.1937795989559436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1562200.086.163768
GTGGTAT1579550.085.25056
AGTGGTA1584650.085.1191255
TGGTATC1582700.084.8158347
GTATCAA1594800.084.366599
GCAGTGG1662050.081.843443
AAGCAGT1663700.081.8077241
CAGTGGT1664200.080.9957664
AGCAGTG1739000.078.327062
CAACGCA1539250.044.8556612-13
ATCAACG1549550.044.6038310-11
ACGCAGA1559950.044.33408414-15
TCAACGC1533850.044.10672412-13
AACGCAG1552400.043.72324814-15
AGAGTAC1574050.043.6287418-19
GAGTACT1535900.043.3127720-21
CGCAGAG1574150.043.1239316-17
TATCAAC1583450.042.4588210-11
AGTACTT1603700.042.29559320-21
GTACTTT1605550.041.37542722-23
Produced by FastQC (version 0.11.5)