##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660775_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25290588 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933995880206503 34.0 31.0 34.0 30.0 34.0 2 32.14120596958837 34.0 31.0 34.0 30.0 34.0 3 32.16925430915248 34.0 31.0 34.0 30.0 34.0 4 35.60537319258848 37.0 35.0 37.0 33.0 37.0 5 35.62627784692076 37.0 35.0 37.0 33.0 37.0 6 35.66615489525194 37.0 35.0 37.0 33.0 37.0 7 35.58362221550563 37.0 35.0 37.0 33.0 37.0 8 35.550895574274506 37.0 35.0 37.0 33.0 37.0 9 37.24666172253488 39.0 37.0 39.0 34.0 39.0 10-11 37.18700526061316 39.0 37.0 39.0 33.5 39.0 12-13 37.28241221595955 39.0 37.0 39.0 34.0 39.0 14-15 38.713071024683174 40.0 38.0 41.0 34.0 41.0 16-17 38.697168586985796 40.0 38.0 41.0 34.0 41.0 18-19 38.66881902864417 40.0 38.0 41.0 34.0 41.0 20-21 38.600713415599515 40.0 38.0 41.0 34.0 41.0 22-23 38.41755349855843 40.0 38.0 41.0 34.0 41.0 24-25 38.45017456691794 40.0 38.0 41.0 34.0 41.0 26-27 38.4650138620739 40.0 38.0 41.0 34.0 41.0 28-29 38.42700721311817 40.0 38.0 41.0 34.0 41.0 30-31 38.345374591527886 40.0 38.0 41.0 34.0 41.0 32-33 38.24063143569458 40.0 38.0 41.0 33.0 41.0 34-35 38.137588101945276 40.0 38.0 41.0 33.0 41.0 36-37 37.98506764650945 40.0 38.0 41.0 33.0 41.0 38-39 37.620999223110196 40.0 36.5 41.0 33.0 41.0 40-41 37.4067916886709 40.0 36.0 41.0 32.0 41.0 42-43 37.34067456241033 40.0 36.0 41.0 32.0 41.0 44-45 37.23138665261558 40.0 35.5 41.0 31.5 41.0 46-47 37.09808858536623 40.0 35.0 41.0 31.0 41.0 48-49 36.95497356566008 39.5 35.0 41.0 31.0 41.0 50-51 35.60834690755312 38.0 34.0 39.5 29.0 40.0 52-53 35.655376854029655 38.0 34.5 39.5 29.0 40.0 54-55 36.249752180534514 39.0 35.0 40.5 28.5 41.0 56-57 36.14370031649719 39.0 35.0 41.0 27.5 41.0 58-59 35.977748955461216 39.0 35.0 41.0 27.0 41.0 60-61 35.7877723127671 39.0 35.0 41.0 26.5 41.0 62-63 35.54364429961059 39.0 35.0 41.0 26.0 41.0 64-65 35.27164487041583 38.0 35.0 41.0 26.0 41.0 66-67 34.99017792706125 37.0 34.5 40.0 26.0 41.0 68-69 34.69969830278363 37.0 34.0 40.0 26.0 41.0 70-71 34.38836400324105 36.0 34.0 39.0 26.0 41.0 72-73 34.03954801288131 36.0 34.0 39.0 25.5 41.0 74-75 33.6704900455458 35.0 34.0 38.5 25.0 40.5 76-77 33.297170650994744 35.0 33.5 37.0 24.0 39.5 78-79 32.937687490698124 35.0 33.0 37.0 24.0 39.0 80-81 32.52267112176277 35.0 33.0 36.0 23.0 39.0 82-83 32.25996910392119 35.0 33.0 36.0 22.5 37.5 84-85 31.99679254195276 35.0 33.0 35.5 20.5 37.0 86-87 31.78213385153402 35.0 32.5 35.0 20.0 37.0 88-89 31.586281347037087 35.0 32.0 35.0 20.0 36.0 90-91 31.4042686552009 35.0 32.0 35.0 20.0 36.0 92-93 31.268523511592534 35.0 32.0 35.0 19.5 36.0 94-95 31.08979648871746 35.0 32.0 35.0 19.0 35.5 96-97 31.020743744668966 35.0 32.0 35.0 19.0 35.0 98-99 30.904491880536746 35.0 31.5 35.0 18.0 35.0 100 30.818383344823776 35.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5112649364247552 1101 2 0.5298308110476313 1101 3 0.5014330054713412 1101 4 0.4294288271525346 1101 5 0.4048951083053254 1101 6 0.39628591063248564 1101 7 0.390319231988407 1101 8 0.4657008218157159 1101 9 0.5974005312206785 1101 10-11 0.5347511062156585 1101 12-13 0.5230025515553578 1101 14-15 0.5994007065281934 1101 16-17 0.5983998633097727 1101 18-19 0.596077433852237 1101 20-21 0.5662235288579041 1101 22-23 0.5594174662907392 1101 24-25 0.5837723354582849 1101 26-27 0.5867841007711121 1101 28-29 0.5398079073986892 1101 30-31 0.5366630491202002 1101 32-33 0.5227540900064795 1101 34-35 0.5026828653818995 1101 36-37 0.557588987408927 1101 38-39 0.5503218750666861 1101 40-41 0.6152892642001646 1101 42-43 0.5552115909042783 1101 44-45 0.49100897985184844 1101 46-47 0.5033797770846178 1101 48-49 0.4257307806971937 1101 50-51 0.3897195820300894 1101 52-53 0.5427931105105586 1101 54-55 0.6354917893980669 1101 56-57 0.5580817413536252 1101 58-59 0.588467980345122 1101 60-61 0.5448985761310823 1101 62-63 0.5387605508692062 1101 64-65 0.5257940728674697 1101 66-67 0.5417470592965472 1101 68-69 0.5043048240534844 1101 70-71 0.4287334182243683 1101 72-73 0.424670182634344 1101 74-75 0.43404123939704675 1101 76-77 0.4012438330890973 1101 78-79 0.3805365364835964 1101 80-81 0.3671015117905867 1101 82-83 0.36498188680889143 1101 84-85 0.3173477397073228 1101 86-87 0.33774585879631047 1101 88-89 0.3573712024134714 1101 90-91 0.35358899535278 1101 92-93 0.3237140251735191 1101 94-95 0.3978405432336096 1101 96-97 0.3520420850787964 1101 98-99 0.31279505059971413 1101 100 0.299708273975007 1102 1 0.5230772390018466 1102 2 0.5113374755621081 1102 3 0.47903603062954403 1102 4 0.4059255535109756 1102 5 0.3942985715200109 1102 6 0.39719543747728636 1102 7 0.4017721703718422 1102 8 0.4863291819080402 1102 9 0.5278841488744277 1102 10-11 0.5040051554104039 1102 12-13 0.49815699650904577 1102 14-15 0.5811725024301566 1102 16-17 0.5525200861318353 1102 18-19 0.5509703645066466 1102 20-21 0.5523073769684785 1102 22-23 0.5498556523740206 1102 24-25 0.5211706298892622 1102 26-27 0.5312605949996794 1102 28-29 0.5208234903331004 1102 30-31 0.5129333893503443 1102 32-33 0.49971346197627753 1102 34-35 0.4839622836787072 1102 36-37 0.47261571498092536 1102 38-39 0.4457679433389288 1102 40-41 0.5687040723472165 1102 42-43 0.46895745098776587 1102 44-45 0.3866686507321617 1102 46-47 0.45464916886004403 1102 48-49 0.4013337151336387 1102 50-51 0.2590391629662605 1102 52-53 0.43749135463220057 1102 54-55 0.5214564856745909 1102 56-57 0.5723631307867763 1102 58-59 0.6145516576160261 1102 60-61 0.6255296932222336 1102 62-63 0.5306664853680942 1102 64-65 0.5208562467257067 1102 66-67 0.5444100845254383 1102 68-69 0.5217977283161161 1102 70-71 0.4923480204212467 1102 72-73 0.4741164500231747 1102 74-75 0.435200215725736 1102 76-77 0.44854937336072 1102 78-79 0.39789440671378173 1102 80-81 0.42134510441926665 1102 82-83 0.41862567313441446 1102 84-85 0.38339165846304724 1102 86-87 0.37378303822156056 1102 88-89 0.4057081194525445 1102 90-91 0.4167581065976833 1102 92-93 0.3966623349800713 1102 94-95 0.45095393718164445 1102 96-97 0.3460220603129329 1102 98-99 0.39802855308395735 1102 100 0.3595673253553322 1103 1 0.37478077253316755 1103 2 0.35403193505158015 1103 3 0.375528138407212 1103 4 0.303675080760911 1103 5 0.27172557397087616 1103 6 0.2641277847074761 1103 7 0.2564102968352131 1103 8 0.37128809749127356 1103 9 0.41974668842232177 1103 10-11 0.33351212238220995 1103 12-13 0.3540439708597134 1103 14-15 0.3765837983194089 1103 16-17 0.3945940692352181 1103 18-19 0.35711325807968564 1103 20-21 0.35985890327580705 1103 22-23 0.4308059189342899 1103 24-25 0.3638681757723887 1103 26-27 0.31694274140288314 1103 28-29 0.32179540615354796 1103 30-31 0.3537055507471507 1103 32-33 0.31194475324594606 1103 34-35 0.34319127940136696 1103 36-37 0.3435120597740351 1103 38-39 0.3469017846773781 1103 40-41 0.41217884647527114 1103 42-43 0.3656209259332357 1103 44-45 0.311566902102129 1103 46-47 0.33362740344286834 1103 48-49 0.3473973437979794 1103 50-51 0.17198320199857164 1103 52-53 0.3095406933161726 1103 54-55 0.4253787646302811 1103 56-57 0.4838378995742758 1103 58-59 0.43845427666692416 1103 60-61 0.4334731269920482 1103 62-63 0.3749479475576649 1103 64-65 0.36821130527372503 1103 66-67 0.35607699077517196 1103 68-69 0.35540862864851874 1103 70-71 0.3129079282789391 1103 72-73 0.35659553842665304 1103 74-75 0.33027719309826864 1103 76-77 0.30925992482161035 1103 78-79 0.27496676317422697 1103 80-81 0.3073558660753619 1103 82-83 0.3227122363516344 1103 84-85 0.252011197137314 1103 86-87 0.24325165335872612 1103 88-89 0.2373384874298914 1103 90-91 0.2692187789998073 1103 92-93 0.25933859876592535 1103 94-95 0.3126164374764855 1103 96-97 0.2986052138106139 1103 98-99 0.27872599794165254 1103 100 0.23231933818689754 1104 1 0.35203418367482087 1104 2 0.32380052278647753 1104 3 0.2898384079802696 1104 4 0.293560571574389 1104 5 0.27048201624690904 1104 6 0.2743093467576614 1104 7 0.2808182699932189 1104 8 0.3484241943090467 1104 9 0.41410341969589837 1104 10-11 0.33938163658248044 1104 12-13 0.35691266374696085 1104 14-15 0.4272058289898055 1104 16-17 0.38264341528209656 1104 18-19 0.35600432389800574 1104 20-21 0.3442844798880529 1104 22-23 0.37427548574122227 1104 24-25 0.41888802992612995 1104 26-27 0.3731070501753919 1104 28-29 0.3651007174561087 1104 30-31 0.3735319136881614 1104 32-33 0.374055624164221 1104 34-35 0.38861564827699624 1104 36-37 0.3424859049979929 1104 38-39 0.37237091940946954 1104 40-41 0.4592086944246674 1104 42-43 0.3720798096242035 1104 44-45 0.38473821324481605 1104 46-47 0.3816371368945184 1104 48-49 0.35242580954886193 1104 50-51 0.28860461636892154 1104 52-53 0.409466777733698 1104 54-55 0.5128169578796076 1104 56-57 0.5339833024375409 1104 58-59 0.5068477090124404 1104 60-61 0.5166938028563592 1104 62-63 0.4713542828000854 1104 64-65 0.4456408992031342 1104 66-67 0.483558296464345 1104 68-69 0.42265336363319506 1104 70-71 0.4048975460552384 1104 72-73 0.415030442907792 1104 74-75 0.3952644698414005 1104 76-77 0.39700660007988375 1104 78-79 0.37394104937609285 1104 80-81 0.3879036791602246 1104 82-83 0.4132500533524279 1104 84-85 0.3956984224589135 1104 86-87 0.4116578707230758 1104 88-89 0.37385476456369204 1104 90-91 0.39696134740067635 1104 92-93 0.3653390829897418 1104 94-95 0.4287882736083901 1104 96-97 0.38907179366866274 1104 98-99 0.3474533979800398 1104 100 0.34420456412965805 1105 1 0.2927618735823039 1105 2 0.2905392764670296 1105 3 0.29486776064619846 1105 4 0.25735380429593135 1105 5 0.2790235533610499 1105 6 0.29696140431659046 1105 7 0.2581521228810928 1105 8 0.35520745932061004 1105 9 0.42504097353715053 1105 10-11 0.3612721471852538 1105 12-13 0.35019181864235094 1105 14-15 0.3711164270677898 1105 16-17 0.3575502668600663 1105 18-19 0.34911360370050915 1105 20-21 0.33024553794837175 1105 22-23 0.355645490479759 1105 24-25 0.3277160504772567 1105 26-27 0.30229963531215276 1105 28-29 0.32784519743260887 1105 30-31 0.32380391783609497 1105 32-33 0.3000997653588584 1105 34-35 0.28190682203531736 1105 36-37 0.32355644844550113 1105 38-39 0.32087951844330576 1105 40-41 0.43133152256434215 1105 42-43 0.31661352071817106 1105 44-45 0.32548405714913287 1105 46-47 0.29107638021541504 1105 48-49 0.2877012814521436 1105 50-51 0.28687249892232813 1105 52-53 0.3482373544847803 1105 54-55 0.4274172830288876 1105 56-57 0.3721478694758318 1105 58-59 0.3462865277038887 1105 60-61 0.3496649085166794 1105 62-63 0.34869314280275177 1105 64-65 0.34646389891516804 1105 66-67 0.304160033382189 1105 68-69 0.31088631007863654 1105 70-71 0.2869373256433292 1105 72-73 0.22369946483276948 1105 74-75 0.2441565083330346 1105 76-77 0.2392625172997711 1105 78-79 0.2622798015272352 1105 80-81 0.28228160976591 1105 82-83 0.31909180553703465 1105 84-85 0.26295405446832376 1105 86-87 0.29252762676894406 1105 88-89 0.28064035361325423 1105 90-91 0.29487358763823934 1105 92-93 0.2812261348364551 1105 94-95 0.3783238946023033 1105 96-97 0.341017542314237 1105 98-99 0.3567388487374892 1105 100 0.30513619149916593 1106 1 0.18910553818804132 1106 2 0.19595037441649055 1106 3 0.2038560094123696 1106 4 0.20771815604661015 1106 5 0.20042344653032984 1106 6 0.1901411524663459 1106 7 0.17757586100525202 1106 8 0.2646418393228629 1106 9 0.29832954913406695 1106 10-11 0.2441451759219504 1106 12-13 0.24783373130961905 1106 14-15 0.23050735287956314 1106 16-17 0.263752362054511 1106 18-19 0.2363617355481651 1106 20-21 0.26971655648291915 1106 22-23 0.2397916159632274 1106 24-25 0.21102950281315458 1106 26-27 0.2460600662542589 1106 28-29 0.2471361405311967 1106 30-31 0.23801435700097073 1106 32-33 0.22576353161768026 1106 34-35 0.18873979604136082 1106 36-37 0.2078969708194549 1106 38-39 0.2223522671678353 1106 40-41 0.34608933643526285 1106 42-43 0.26108917948172206 1106 44-45 0.21533278272447376 1106 46-47 0.20405674385585115 1106 48-49 0.20937985887637467 1106 50-51 0.11906437740324094 1106 52-53 0.177908741969091 1106 54-55 0.26728529853126304 1106 56-57 0.32811858562891416 1106 58-59 0.3411194591528641 1106 60-61 0.30289643037225034 1106 62-63 0.3259639547832123 1106 64-65 0.2915783923704467 1106 66-67 0.32997965656560524 1106 68-69 0.30679684627188664 1106 70-71 0.3448906652549226 1106 72-73 0.27939855984089235 1106 74-75 0.23496361939007215 1106 76-77 0.26148869352914517 1106 78-79 0.257681549395663 1106 80-81 0.27478877346666053 1106 82-83 0.26889604374534315 1106 84-85 0.30749813162524475 1106 86-87 0.33838398537668013 1106 88-89 0.30518316682233504 1106 90-91 0.2946399335197576 1106 92-93 0.2841907227716156 1106 94-95 0.30411874794707217 1106 96-97 0.29472481782373805 1106 98-99 0.24886402938383156 1106 100 0.27412717325883307 1107 1 0.14594658006744154 1107 2 0.15309458475763194 1107 3 0.17681130078198493 1107 4 0.1699946311108107 1107 5 0.1576105948939528 1107 6 0.17865822410362142 1107 7 0.2159015591775031 1107 8 0.27600792421835507 1107 9 0.2813227092372017 1107 10-11 0.25642766230928515 1107 12-13 0.25434404632376584 1107 14-15 0.2777737882534126 1107 16-17 0.24680095124121237 1107 18-19 0.24262301003195574 1107 20-21 0.23527766015143925 1107 22-23 0.24903890462228162 1107 24-25 0.19696391745601005 1107 26-27 0.19740933505877933 1107 28-29 0.22534938792603043 1107 30-31 0.20472112517833096 1107 32-33 0.15436072300258985 1107 34-35 0.14021501637847678 1107 36-37 0.17848884933972187 1107 38-39 0.1784372095059723 1107 40-41 0.30105619869113553 1107 42-43 0.19770511162194992 1107 44-45 0.18135878807272832 1107 46-47 0.22272593579869948 1107 48-49 0.22685914713571265 1107 50-51 0.15236970994130417 1107 52-53 0.2411003694190299 1107 54-55 0.30628759584558196 1107 56-57 0.3103739924367517 1107 58-59 0.2419516240515307 1107 60-61 0.2771796776734021 1107 62-63 0.27023170275940345 1107 64-65 0.2761166707066849 1107 66-67 0.2255374709213669 1107 68-69 0.23038408124019583 1107 70-71 0.2204953106805334 1107 72-73 0.24827174642506833 1107 74-75 0.2630273904205964 1107 76-77 0.250916701819051 1107 78-79 0.24235892090167255 1107 80-81 0.2085017049417317 1107 82-83 0.2959818687851268 1107 84-85 0.2712291647296823 1107 86-87 0.24291493562374455 1107 88-89 0.31252895166172223 1107 90-91 0.3092909072500163 1107 92-93 0.3010190445871501 1107 94-95 0.3707019587118694 1107 96-97 0.3622971644881581 1107 98-99 0.33548576466285596 1107 100 0.34325322576084716 1108 1 0.1522332107003983 1108 2 0.14749686655867578 1108 3 0.13875529370144335 1108 4 0.13100196527398822 1108 5 0.1234458949913062 1108 6 0.12415676848565482 1108 7 0.13612540182533195 1108 8 0.19008509611446556 1108 9 0.20925639685211195 1108 10-11 0.15674283473767758 1108 12-13 0.19618929386385986 1108 14-15 0.18499757053541543 1108 16-17 0.19872767043562334 1108 18-19 0.19778458177166414 1108 20-21 0.16280722985848683 1108 22-23 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-0.7459907723252712 2316 64-65 -0.7818779131762454 2316 66-67 -0.7871942476931295 2316 68-69 -0.7189498894319613 2316 70-71 -0.7056681664577553 2316 72-73 -0.6739161594974377 2316 74-75 -0.6671736230223289 2316 76-77 -0.6736969113597624 2316 78-79 -0.6604877208660795 2316 80-81 -0.8271517232818653 2316 82-83 -0.8248590111778462 2316 84-85 -0.6245364726984732 2316 86-87 -0.6497277576064135 2316 88-89 -0.8166108964162113 2316 90-91 -0.7596426381537711 2316 92-93 -0.6653540731282241 2316 94-95 -0.5829292182262833 2316 96-97 -0.5267674311342141 2316 98-99 -0.5686487444306856 2316 100 -0.9153740650303988 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 6.0 6 377.0 7 4822.0 8 12224.0 9 18924.0 10 23283.0 11 23841.0 12 24709.0 13 27553.0 14 32490.0 15 41592.0 16 55061.0 17 72689.0 18 78050.0 19 86245.0 20 98111.0 21 120360.0 22 157982.0 23 234752.0 24 341694.0 25 414785.0 26 411914.0 27 361030.0 28 322874.0 29 332190.0 30 381534.0 31 466612.0 32 586040.0 33 763712.0 34 1053959.0 35 1622465.0 36 2773013.0 37 5308126.0 38 6893970.0 39 2138505.0 40 5093.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.285173911407473 33.93634503833121 27.95706032982071 21.821420720440607 2 20.98808100163467 32.89274421685959 27.033484194853685 19.085690586652063 3 25.42228060456435 25.989830422883347 26.560277195814425 22.02761177673788 4 19.789938851794787 27.529164951337258 25.75488240405696 26.926013792810995 5 21.300189335174515 31.98757873293473 26.32938340236388 20.38284852952687 6 26.97627038714551 26.673493905811075 26.142910325173563 20.20732538186985 7 20.884927473470547 26.36326443041141 31.832248228486005 20.91955986763204 8 26.50082523276468 26.66021270777388 26.10667104839856 20.732291011062884 9 26.082807666037205 25.918981854235955 27.05488747993849 20.943322999788354 10-11 20.55630046078233 29.590834084313006 29.181672913944944 20.671192540959716 12-13 20.560290834790944 26.749895824823422 29.085865494642878 23.60394784574276 14-15 20.553327532906188 32.54131173156986 26.237603119299813 20.66775761622414 16-17 23.50353569422201 26.645111392488452 26.264119080901793 23.587233832387742 18-19 20.605616479604233 29.550089295299646 26.2373513153683 23.60694290972782 20-21 23.525979041444852 29.599310829393595 26.195047928904184 20.67966220025736 22-23 23.527960958817022 26.762843159326394 29.027896831218097 20.681299050638486 24-25 20.68840359254821 29.557378702121333 26.07426173410316 23.679955971227294 26-27 20.702058553119418 26.7381963106353 31.846368409593552 20.713376726651735 28-29 20.72820409433733 26.801624175485994 31.827178835242492 20.64299289493419 30-31 20.71459675830393 26.77531077697734 31.793467268987367 20.71662519573136 32-33 20.76985558362375 26.828366482194216 31.68964342959609 20.712134504585944 34-35 20.764007147639273 26.92883415759254 31.634371253052716 20.67278744171547 36-37 20.74983784481405 26.890948522035153 31.640579096065302 20.718634537085496 38-39 20.79227853460742 26.88406058411928 31.63514031385905 20.688520567414248 40-41 20.883211334368777 26.912674830139842 31.568389871273627 20.63572396421775 42-43 20.859991969343533 26.983927022644988 31.51934405629605 20.63673695171543 44-45 20.941451602173103 27.044116933295992 31.44153814627961 20.5728933182513 46-47 20.948583780254594 27.147862403410894 31.364699098059496 20.538854718275015 48-49 21.02710370820678 27.162680677958996 31.24679865493334 20.563416958900884 50-51 21.098692797701844 27.198239897802456 31.154527060228688 20.548540244267013 52-53 21.23136367118085 27.34264049610749 30.923033685626887 20.502962147084773 54-55 21.58126733484158 27.551791886129617 30.248104876883925 20.618835902144877 56-57 22.05409664868593 28.011949663327307 29.211403831789273 20.722549856197485 58-59 22.24415857788676 28.484567065028305 28.592213830694647 20.67906052639029 60-61 22.42456165906463 28.71852564282017 28.22180923591021 20.63510346220499 62-63 22.576347374762502 28.848854759723263 28.018937321662907 20.555860543851335 64-65 22.76042375922616 28.915154127693672 27.912897873311604 20.411524239768568 66-67 22.913552612563134 28.754200426759603 28.005170698898425 20.32707626177884 68-69 23.065030084117858 28.61285109752494 28.132751525300698 20.189367293056502 70-71 23.241901295454262 28.510651867801574 28.224671960968244 20.02277487577592 72-73 23.4318374742568 28.394089213786287 28.216713986761338 19.957359325195572 74-75 23.660249005072142 28.43550133934022 28.078008473508053 19.82624118207958 76-77 23.96321344550789 28.518183203049368 27.78780362681711 19.73079972462563 78-79 24.23052604514396 28.60803021478032 27.481675688568906 19.67976805150681 80-81 24.51797658450245 28.723171337139387 27.164062745857276 19.59478933250089 82-83 24.70064947936745 28.836911198883737 26.912829215385 19.54961010636382 84-85 24.895405737087156 28.81309681581714 26.76067833026251 19.530819116833193 86-87 25.08332728448565 28.76107940126433 26.666917913818445 19.488675400431575 88-89 25.269433929434697 28.741784863724483 26.544900382297083 19.44388082454373 90-91 25.47435468677356 28.68478792653259 26.414806573841037 19.426050812852814 92-93 25.597763827988366 28.682328963192976 26.3568887301248 19.363018478693853 94-95 25.742713638699673 28.667547394097937 26.258739350003857 19.330999617198533 96-97 25.853964109077076 28.647543303674787 26.17190360111616 19.326588986131977 98-99 25.96795784137153 28.67307177090112 26.10011487754819 19.258855510179156 100 26.01674667165259 28.742424914701818 25.989823932085095 19.251004481560493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52764.0 1 33067.5 2 10691.5 3 7727.5 4 7331.0 5 7237.0 6 7432.5 7 7616.5 8 7630.5 9 7824.0 10 8509.0 11 11379.5 12 100097.0 13 120700.0 14 53561.5 15 55567.0 16 62482.5 17 69323.5 18 75765.5 19 82929.0 20 92369.0 21 106169.0 22 125004.5 23 147614.5 24 171622.0 25 198988.0 26 229914.5 27 266354.0 28 309439.5 29 350191.0 30 385757.5 31 424517.0 32 468027.5 33 505977.0 34 540760.5 35 584041.0 36 628603.0 37 665741.5 38 706602.5 39 740995.0 40 763638.0 41 794837.5 42 831309.5 43 841714.0 44 837228.5 45 847679.0 46 861903.5 47 870440.0 48 872200.0 49 868676.5 50 860248.0 51 848945.0 52 831533.5 53 795022.0 54 745453.5 55 695250.0 56 641735.5 57 580342.0 58 510778.5 59 432940.5 60 352500.5 61 282755.5 62 223185.5 63 168837.5 64 124875.0 65 92124.0 66 68262.5 67 51765.5 68 39886.5 69 30942.5 70 24171.5 71 19348.0 72 15706.0 73 12501.0 74 9875.0 75 8199.5 76 6418.0 77 4163.0 78 2645.0 79 1890.5 80 1335.5 81 994.0 82 791.5 83 605.0 84 503.5 85 432.0 86 339.0 87 278.5 88 243.5 89 207.0 90 190.5 91 166.5 92 137.5 93 114.0 94 97.0 95 78.0 96 65.0 97 52.0 98 38.0 99 25.5 100 15.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17215099941527656 2 0.12257129015742932 3 0.08965390603018007 4 0.044233846994779245 5 0.027104945128203425 6 0.02213867071813435 7 0.019821603198786838 8 0.014958133832238303 9 0.013301391015503474 10-11 0.009920686699731932 12-13 0.005749174356879326 14-15 0.008779946120667499 16-17 0.005911290002430944 18-19 0.0035704982422709985 20-21 0.01795727327494323 22-23 0.08167860707706756 24-25 0.020974205898257485 26-27 0.015114318417586812 28-29 0.013518863222950768 30-31 7.314974250499831E-4 32-33 1.977020067702657E-6 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.779318060932391E-5 42-43 1.186212040621594E-5 44-45 6.128762209878236E-5 46-47 0.0013404196059024014 48-49 0.013307322075706583 50-51 0.015319928504627886 52-53 0.010600781603021645 54-55 0.004752756242757187 56-57 3.954040135405314E-6 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.186212040621594E-5 68-69 3.954040135405314E-6 70-71 0.0 72-73 0.0016448806963286105 74-75 0.011666395419513377 76-77 0.016939107940076362 78-79 0.02375982717365053 80-81 0.04875133784947981 82-83 0.004962320369933668 84-85 0.003795878529989101 86-87 0.01345362156071658 88-89 0.01052960888058435 90-91 0.005596943811666222 92-93 0.008673187037011556 94-95 0.007997046173857245 96-97 0.013390356918550094 98-99 0.02480764780953294 100 0.013499093022273742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5290588E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.251911430915065 #Duplication Level Percentage of deduplicated Percentage of total 1 77.44308154749618 38.14219793314415 2 12.060113285924807 11.879672628103414 3 3.972530370887411 5.869641419507008 4 1.8739308497365044 3.6917870495552694 5 1.1024368600013603 2.7148561293481555 6 0.7306151008264095 2.1590514141594843 7 0.4760223210576928 1.6411506437110472 8 0.36309034119954947 1.4306314660944754 9 0.2773306564503258 1.2293158435712084 >10 1.4742684360776617 13.334757023545835 >50 0.13291997924136384 4.705465838923888 >100 0.09283397424678404 7.302324659451558 >500 2.8016269022349124E-4 0.09586243586386926 >1k 5.140670270205728E-4 0.48387918161532045 >5k 0.0 0.0 >10k+ 3.2047136808005035E-5 5.319406333405139 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1259099 4.978527980448695 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49008 0.1937795989559436 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.01954877442944387 0.0 3.954040135405314E-6 0.0 7.908080270810628E-6 2 0.019572498670256303 2.7678280947837194E-5 3.954040135405314E-6 0.0 1.186212040621594E-5 3 0.019608085031474952 4.744848162486376E-5 3.954040135405314E-6 0.0 1.186212040621594E-5 4 0.019647625432829004 7.512676257270096E-5 3.954040135405314E-6 0.0 1.186212040621594E-5 5 0.019663441593370624 9.489696324972752E-5 3.954040135405314E-6 0.0 1.186212040621594E-5 6 0.019706936034860085 1.146671639267541E-4 3.954040135405314E-6 0.0 1.186212040621594E-5 7 0.01973461431580792 1.2257524419756471E-4 3.954040135405314E-6 0.0 1.977020067702657E-5 8 0.01975438451648495 1.2257524419756471E-4 3.954040135405314E-6 0.0 1.977020067702657E-5 9 0.01977810875729738 1.2257524419756471E-4 3.954040135405314E-6 3.954040135405314E-6 2.7678280947837194E-5 10-11 0.019833465319193055 1.3839140473918598E-4 3.954040135405314E-6 3.954040135405314E-6 3.163232108324251E-5 12-13 0.01988091380081792 1.5025352514540192E-4 3.954040135405314E-6 3.954040135405314E-6 3.163232108324251E-5 14-15 0.019938247382781292 1.6211564555161786E-4 3.954040135405314E-6 3.954040135405314E-6 3.558636121864782E-5 16-17 0.020019305205557104 1.779318060932391E-4 3.954040135405314E-6 3.954040135405314E-6 3.558636121864782E-5 18-19 0.02012606428921305 1.9770200677026568E-4 3.954040135405314E-6 3.954040135405314E-6 3.756338128635048E-5 20-21 0.020201191051785746 2.332883679889135E-4 3.954040135405314E-6 7.908080270810628E-6 4.54714615571611E-5 22-23 0.020337605436457233 2.570126088013454E-4 3.954040135405314E-6 1.186212040621594E-5 4.744848162486376E-5 24-25 0.020944550597241946 2.7678280947837197E-4 3.954040135405314E-6 1.186212040621594E-5 5.535656189567439E-5 26-27 0.022909708544538387 3.0050705029080384E-4 3.954040135405314E-6 1.186212040621594E-5 5.535656189567439E-5 28-29 0.02934688588497824 3.103921506293171E-4 3.954040135405314E-6 1.3839140473918597E-5 5.535656189567439E-5 30-31 0.04538051863404678 3.242312911032357E-4 3.954040135405314E-6 1.5816160541621256E-5 5.535656189567439E-5 32-33 0.07840268482488427 3.6574871252499153E-4 7.908080270810628E-6 1.977020067702657E-5 5.535656189567439E-5 34-35 0.1378338850800938 3.91449973405126E-4 7.908080270810628E-6 1.977020067702657E-5 6.524166223418767E-5 36-37 0.23130146282087233 4.0133507374363935E-4 7.908080270810628E-6 1.977020067702657E-5 7.117272243729564E-5 38-39 0.36408208460791813 4.131971941498553E-4 7.908080270810628E-6 1.977020067702657E-5 7.512676257270096E-5 40-41 0.5426682843435668 4.290133546914765E-4 7.908080270810628E-6 2.1747220744729225E-5 8.105782277580893E-5 42-43 0.7700552474303879 4.586686557070164E-4 7.908080270810628E-6 2.372424081243188E-5 9.094292311432222E-5 44-45 1.0468677122097754 4.843699165871509E-4 7.908080270810628E-6 2.372424081243188E-5 9.094292311432222E-5 46-47 1.3778722740649605 4.942550169256642E-4 7.908080270810628E-6 2.372424081243188E-5 9.094292311432222E-5 48-49 1.7648660442374848 5.041401172641775E-4 7.908080270810628E-6 2.372424081243188E-5 9.094292311432222E-5 50-51 2.202086009229995 5.140252176026908E-4 7.908080270810628E-6 2.372424081243188E-5 9.885100338513284E-5 52-53 2.688452716085526 5.23910317941204E-4 7.908080270810628E-6 2.372424081243188E-5 1.1071312379134878E-4 54-55 3.2250377096807714 5.496115788213386E-4 7.908080270810628E-6 2.372424081243188E-5 1.1071312379134878E-4 56-57 3.810330546684008 5.693817794983651E-4 7.908080270810628E-6 2.372424081243188E-5 1.1071312379134878E-4 58-59 4.439643712514711 6.029911206493103E-4 7.908080270810628E-6 2.372424081243188E-5 1.1862120406215941E-4 60-61 5.110403127044734 6.286923815294449E-4 7.908080270810628E-6 2.5701260880134535E-5 1.285063044006727E-4 62-63 5.815353126625605 6.524166223418767E-4 7.908080270810628E-6 2.7678280947837194E-5 1.3048332446837535E-4 64-65 6.549262516158185 6.80094903289714E-4 7.908080270810628E-6 3.163232108324251E-5 1.3048332446837535E-4 66-67 7.3126828842413625 6.939340437636325E-4 7.908080270810628E-6 3.954040135405314E-5 1.3048332446837535E-4 68-69 8.106118370992402 6.978880838990379E-4 7.908080270810628E-6 3.954040135405314E-5 1.3641438467148332E-4 70-71 8.924234976268643 7.038191441021459E-4 7.908080270810628E-6 4.15174214217558E-5 1.423454448745913E-4 72-73 9.759041584956428 7.43359545456199E-4 7.908080270810628E-6 4.349444148945845E-5 1.462994850099966E-4 74-75 10.615644839890635 7.749918665394415E-4 7.908080270810628E-6 4.744848162486376E-5 1.462994850099966E-4 76-77 11.488507107861627 7.987161073518734E-4 7.908080270810628E-6 5.140252176026908E-5 1.462994850099966E-4 78-79 12.380139204355391 8.204633280966026E-4 7.908080270810628E-6 5.140252176026908E-5 1.5025352514540192E-4 80-81 13.28427990681751 8.362794886382238E-4 7.908080270810628E-6 5.140252176026908E-5 1.5420756528080723E-4 82-83 14.19952750801998 8.560496893152504E-4 7.908080270810628E-6 5.3379541827971736E-5 1.6211564555161786E-4 84-85 15.12084258381023 8.876820103984929E-4 7.908080270810628E-6 5.733358196337705E-5 1.6211564555161786E-4 86-87 16.0518648281329 9.173373114140327E-4 7.908080270810628E-6 5.9310602031079705E-5 1.6211564555161786E-4 88 16.75530043034191 9.291994318202487E-4 7.908080270810628E-6 6.326464216648502E-5 1.6211564555161786E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 156220 0.0 86.16376 8 GTGGTAT 157955 0.0 85.2505 6 AGTGGTA 158465 0.0 85.119125 5 TGGTATC 158270 0.0 84.815834 7 GTATCAA 159480 0.0 84.36659 9 GCAGTGG 166205 0.0 81.84344 3 AAGCAGT 166370 0.0 81.807724 1 CAGTGGT 166420 0.0 80.995766 4 AGCAGTG 173900 0.0 78.32706 2 CAACGCA 153925 0.0 44.85566 12-13 ATCAACG 154955 0.0 44.60383 10-11 ACGCAGA 155995 0.0 44.334084 14-15 TCAACGC 153385 0.0 44.106724 12-13 AACGCAG 155240 0.0 43.723248 14-15 AGAGTAC 157405 0.0 43.62874 18-19 GAGTACT 153590 0.0 43.31277 20-21 CGCAGAG 157415 0.0 43.12393 16-17 TATCAAC 158345 0.0 42.45882 10-11 AGTACTT 160370 0.0 42.295593 20-21 GTACTTT 160555 0.0 41.375427 22-23 >>END_MODULE