FastQCFastQC Report
Thu 26 May 2016
SRR1660775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25290588
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13443635.315665258553894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389880.15416011679918237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261520.10340605762111975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1682650.086.0434958
GTGGTAT1702350.085.345256
AGTGGTA1705750.085.241125
TGGTATC1702650.084.996997
GTATCAA1703050.084.8448649
GCAGTGG1762150.082.236123
AAGCAGT1760300.082.175741
CAGTGGT1783700.081.466034
AGCAGTG1841900.078.604212
CAACGCA1643700.044.81431612-13
ATCAACG1656800.044.5830910-11
ACGCAGA1663600.044.38253814-15
TCAACGC1639650.044.02559312-13
AACGCAG1652150.043.67263414-15
AGAGTAC1688500.043.4892818-19
GAGTACT1646600.043.2602820-21
CGCAGAG1676200.043.11668416-17
TATCAAC1700200.042.47010410-11
AGTACTT1717000.042.44241320-21
GTACTTT1719000.041.39052622-23
Produced by FastQC (version 0.11.5)