##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660775_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25290588 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.33464730041073 34.0 31.0 34.0 30.0 34.0 2 32.57746953135293 34.0 31.0 34.0 31.0 34.0 3 32.24097901559268 34.0 31.0 34.0 30.0 34.0 4 36.01579544137131 37.0 35.0 37.0 35.0 37.0 5 36.04512919984304 37.0 35.0 37.0 35.0 37.0 6 36.09489557933568 37.0 36.0 37.0 35.0 37.0 7 36.00859185243143 37.0 35.0 37.0 35.0 37.0 8 36.06407854178796 37.0 35.0 37.0 35.0 37.0 9 37.8242476608294 39.0 38.0 39.0 35.0 39.0 10-11 37.702307969272994 39.0 37.5 39.0 35.0 39.0 12-13 37.81594233791638 39.0 38.0 39.0 35.0 39.0 14-15 39.27721644906002 41.0 39.0 41.0 36.0 41.0 16-17 39.26218358386922 41.0 39.0 41.0 36.0 41.0 18-19 39.219732850813905 41.0 39.0 41.0 36.0 41.0 20-21 39.115828465514525 40.5 39.0 41.0 36.0 41.0 22-23 38.97718139254018 40.0 38.5 41.0 35.0 41.0 24-25 39.02703936737255 40.0 39.0 41.0 35.0 41.0 26-27 39.028785728508964 40.0 39.0 41.0 35.0 41.0 28-29 38.94047083840044 40.0 39.0 41.0 35.0 41.0 30-31 38.835790848358286 40.0 38.0 41.0 35.0 41.0 32-33 38.77000851067599 40.0 38.0 41.0 35.0 41.0 34-35 38.682921804744126 40.0 38.0 41.0 35.0 41.0 36-37 38.535329071827036 40.0 38.0 41.0 34.0 41.0 38-39 38.18656041132772 40.0 37.0 41.0 34.0 41.0 40-41 38.09255951265348 40.0 37.0 41.0 33.5 41.0 42-43 37.98571183872831 40.0 37.0 41.0 33.0 41.0 44-45 37.873028080644076 40.0 37.0 41.0 33.0 41.0 46-47 37.74201659526461 40.0 36.0 41.0 33.0 41.0 48-49 37.55776449325734 40.0 36.0 41.0 33.0 41.0 50-51 37.3721032306564 40.0 35.5 41.0 32.5 41.0 52-53 37.05953778140706 39.0 35.0 41.0 31.5 41.0 54-55 36.503031602112216 39.0 35.0 41.0 29.5 41.0 56-57 35.91558581002545 39.0 35.0 41.0 27.5 41.0 58-59 35.649002071442546 39.0 35.0 40.0 27.0 41.0 60-61 35.47732122716957 38.0 35.0 40.0 26.5 41.0 62-63 35.30052644485767 38.0 35.0 40.0 26.5 41.0 64-65 35.22949774833231 38.0 35.0 40.0 26.5 41.0 66-67 35.15905743274929 37.0 35.0 40.0 27.0 41.0 68-69 34.94412158388725 37.0 34.5 40.0 27.0 41.0 70-71 34.652322358025046 36.0 34.0 39.0 26.5 41.0 72-73 34.304764404844995 36.0 34.0 39.0 26.0 41.0 74-75 33.926828589355054 35.0 34.0 38.5 26.0 40.0 76-77 32.24132855274065 34.5 31.5 36.5 24.0 39.0 78-79 33.120085444434906 35.0 33.5 37.0 25.5 39.0 80-81 33.02487118923451 35.0 34.0 36.5 25.5 39.0 82-83 32.754696747264234 35.0 34.0 36.0 25.0 37.5 84-85 32.519158708370085 35.0 33.0 36.0 25.0 37.0 86-87 31.185666046989496 35.0 32.0 35.0 16.0 36.5 88-89 30.787111869443287 35.0 31.0 35.0 15.5 36.0 90-91 31.720538506261697 35.0 32.5 35.0 23.0 36.0 92-93 31.825054700191235 35.0 33.0 35.0 23.0 36.0 94-95 31.752660811998517 35.0 33.0 35.0 23.0 35.5 96-97 31.65546034358711 35.0 33.0 35.0 23.0 35.0 98-99 31.56592594842002 35.0 33.0 35.0 21.5 35.0 100 31.443879992034983 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4708262543791051 1101 2 0.46126330654337266 1101 3 0.8149646511510156 1101 4 0.42818054716430254 1101 5 0.3717872330321583 1101 6 0.3408316826458204 1101 7 0.3247542196149027 1101 8 0.33036996754194803 1101 9 0.3963391860779666 1101 10-11 0.41524807803513397 1101 12-13 0.4221431462643963 1101 14-15 0.5459626234165356 1101 16-17 0.5296106125928901 1101 18-19 0.5292498545449007 1101 20-21 0.5713535989570673 1101 22-23 0.5702283109556845 1101 24-25 0.5312675986696149 1101 26-27 0.5152928318068817 1101 28-29 0.5077671153456791 1101 30-31 0.548642477229329 1101 32-33 0.5300449115150698 1101 34-35 0.5049431456062834 1101 36-37 0.47804250430308315 1101 38-39 0.49625703264863574 1101 40-41 0.49215868886476244 1101 42-43 0.45236558176328145 1101 44-45 0.46397297862906584 1101 46-47 0.4792830761023552 1101 48-49 0.4433396341864153 1101 50-51 0.48674429483278203 1101 52-53 0.4818991824685597 1101 54-55 0.519937662325404 1101 56-57 0.44002960250953294 1101 58-59 0.37774602694849335 1101 60-61 0.3359067629350747 1101 62-63 0.4781127229894153 1101 64-65 0.5100832170393872 1101 66-67 0.5017754828536596 1101 68-69 0.4638020460993886 1101 70-71 0.4229895679754563 1101 72-73 0.37614132278048373 1101 74-75 0.41424601737276845 1101 76-77 0.4213419223118535 1101 78-79 0.4604116897868522 1101 80-81 0.4429977529292586 1101 82-83 0.4401022711859568 1101 84-85 0.43142877345754727 1101 86-87 1.5303248262406832 1101 88-89 1.7867055988686218 1101 90-91 0.73103600602062 1101 92-93 0.4686202433025848 1101 94-95 0.4471088880777785 1101 96-97 0.47061627924881577 1101 98-99 0.43660014093442 1101 100 0.45755111192281817 1102 1 0.3775865686769748 1102 2 0.39350097633738557 1102 3 0.7525820975338675 1102 4 0.40651791192114217 1102 5 0.319405280299371 1102 6 0.29514935489049776 1102 7 0.2965769257388544 1102 8 0.28854304344542925 1102 9 0.37364193400461687 1102 10-11 0.3587122058279206 1102 12-13 0.3334821345872143 1102 14-15 0.4511783467929362 1102 16-17 0.4366501849517306 1102 18-19 0.4492517809094636 1102 20-21 0.525176990796119 1102 22-23 0.5124339041286987 1102 24-25 0.43959331098267995 1102 26-27 0.47162053292935724 1102 28-29 0.5066876678293966 1102 30-31 0.49944372117777647 1102 32-33 0.4493410108330025 1102 34-35 0.40884103532397376 1102 36-37 0.4444016399250401 1102 38-39 0.43813430731994885 1102 40-41 0.47050195642678005 1102 42-43 0.39967422660409824 1102 44-45 0.3634220265808139 1102 46-47 0.4410490045452775 1102 48-49 0.47302796960713067 1102 50-51 0.43839309206259003 1102 52-53 0.42604064832006117 1102 54-55 0.43722587709797267 1102 56-57 0.41073995386193474 1102 58-59 0.4004160566039019 1102 60-61 0.3582349247355623 1102 62-63 0.3593909336264858 1102 64-65 0.3640196163753444 1102 66-67 0.3619926436397094 1102 68-69 0.37040409216954373 1102 70-71 0.3479698742850985 1102 72-73 0.35425683160582366 1102 74-75 0.3251114616914137 1102 76-77 0.3237603110227667 1102 78-79 0.40353902778666395 1102 80-81 0.36337177398848297 1102 82-83 0.34468894522032656 1102 84-85 0.349230791486427 1102 86-87 1.43050725944531 1102 88-89 1.6935337129518189 1102 90-91 0.6426125851977424 1102 92-93 0.40127297691405417 1102 94-95 0.3426561876916878 1102 96-97 0.3329618018944984 1102 98-99 0.32085583961565334 1102 100 0.43988152998516483 1103 1 0.3499317205021768 1103 2 0.345686757208334 1103 3 0.6876834634243849 1103 4 0.3690697163821923 1103 5 0.2960838178249787 1103 6 0.24508241107888296 1103 7 0.2461405581860916 1103 8 0.25138511577518585 1103 9 0.3108797162694472 1103 10-11 0.2723412328818142 1103 12-13 0.29643659659564037 1103 14-15 0.37806769368355475 1103 16-17 0.3691553485973529 1103 18-19 0.3343839239525721 1103 20-21 0.4520037820281786 1103 22-23 0.42805826354069154 1103 24-25 0.33771775829124806 1103 26-27 0.34367381472043235 1103 28-29 0.37356491392066715 1103 30-31 0.35400808795082384 1103 32-33 0.2865150780945811 1103 34-35 0.28292969025715564 1103 36-37 0.2771246224093673 1103 38-39 0.27280514688577995 1103 40-41 0.22661567723788778 1103 42-43 0.2538302147640934 1103 44-45 0.30683709111915647 1103 46-47 0.3158757058780566 1103 48-49 0.2254130620810031 1103 50-51 0.2253295626434877 1103 52-53 0.23731574279219814 1103 54-55 0.33138001831728303 1103 56-57 0.2573405638419146 1103 58-59 0.2218797704500446 1103 60-61 0.2180250432913553 1103 62-63 0.29723225605382453 1103 64-65 0.31833651089811354 1103 66-67 0.3016825289469267 1103 68-69 0.31236281059742765 1103 70-71 0.2753888614584241 1103 72-73 0.2390427311260197 1103 74-75 0.2235625304470048 1103 76-77 0.22810631600105324 1103 78-79 0.2644908141788491 1103 80-81 0.24294944763540371 1103 82-83 0.27816675456175943 1103 84-85 0.25126694568417207 1103 86-87 1.2855175021778216 1103 88-89 1.536030147021151 1103 90-91 0.5090528451223726 1103 92-93 0.29570615304232817 1103 94-95 0.29031690379731856 1103 96-97 0.27527886241940536 1103 98-99 0.21448369842393689 1103 100 0.2770041210070744 1104 1 0.3743775275609522 1104 2 0.37582343438811705 1104 3 0.7050977690336282 1104 4 0.34717469930987477 1104 5 0.2676880680068834 1104 6 0.24253380510236866 1104 7 0.2396108361534246 1104 8 0.23260871874733624 1104 9 0.2549839196183328 1104 10-11 0.2754536788178612 1104 12-13 0.26227952876404004 1104 14-15 0.3182460159658689 1104 16-17 0.3094104912371307 1104 18-19 0.336928873904796 1104 20-21 0.4237716575670376 1104 22-23 0.4138406615513688 1104 24-25 0.35177608206386424 1104 26-27 0.31787255805530634 1104 28-29 0.38775968820374374 1104 30-31 0.37751643066419405 1104 32-33 0.3403090178169279 1104 34-35 0.30469304034366473 1104 36-37 0.28348550752787816 1104 38-39 0.3322224152819828 1104 40-41 0.3207721064031688 1104 42-43 0.3117598926004135 1104 44-45 0.3102657257378567 1104 46-47 0.2808343186390374 1104 48-49 0.29360431690930966 1104 50-51 0.31229071466890446 1104 52-53 0.34336003458069797 1104 54-55 0.38816808534127745 1104 56-57 0.35259744170473795 1104 58-59 0.39876267954660705 1104 60-61 0.36594183772398736 1104 62-63 0.44220822054413844 1104 64-65 0.42564792934393125 1104 66-67 0.4030423344629668 1104 68-69 0.36088022822707444 1104 70-71 0.3044183479061502 1104 72-73 0.30723596534383546 1104 74-75 0.35251159521761366 1104 76-77 0.28073564390385286 1104 78-79 0.35895145964340003 1104 80-81 0.39469206374207033 1104 82-83 0.43080615313068193 1104 84-85 0.42800026889116083 1104 86-87 1.5217086086270761 1104 88-89 1.763290291845486 1104 90-91 0.6851663855605956 1104 92-93 0.4722651500788082 1104 94-95 0.40585662564733127 1104 96-97 0.38674437844328935 1104 98-99 0.37089580296039415 1104 100 0.5160226914139656 1105 1 0.3500993544890463 1105 2 0.3469345890881641 1105 3 0.6884603575535948 1105 4 0.3433322963013694 1105 5 0.26208240365522784 1105 6 0.2407702173602999 1105 7 0.2170376622181962 1105 8 0.2095454179829659 1105 9 0.2535519881460644 1105 10-11 0.2639422593399061 1105 12-13 0.22876437905939184 1105 14-15 0.27109166002234275 1105 16-17 0.27927318756977115 1105 18-19 0.2946341626194737 1105 20-21 0.3229896955244058 1105 22-23 0.3405271927265332 1105 24-25 0.32782346471059043 1105 26-27 0.3099836963732301 1105 28-29 0.3524343592546373 1105 30-31 0.3646348312802914 1105 32-33 0.3204541115384103 1105 34-35 0.26739526331767394 1105 36-37 0.27982291535068526 1105 38-39 0.2502724719440508 1105 40-41 0.27320087881576427 1105 42-43 0.2934168563857824 1105 44-45 0.2871475161272983 1105 46-47 0.2867199749749574 1105 48-49 0.3127151385736582 1105 50-51 0.2909054637592945 1105 52-53 0.3419115577919598 1105 54-55 0.3481462567920772 1105 56-57 0.382833418872913 1105 58-59 0.32037941567588746 1105 60-61 0.33010637835505463 1105 62-63 0.3979303735602926 1105 64-65 0.3723596887461511 1105 66-67 0.3043313560556342 1105 68-69 0.2985275283663711 1105 70-71 0.25909575044968847 1105 72-73 0.26392607765910014 1105 74-75 0.27550866459055356 1105 76-77 0.29567452205430556 1105 78-79 0.32269965320025307 1105 80-81 0.3232717941144969 1105 82-83 0.3516743302606571 1105 84-85 0.3160542574167451 1105 86-87 1.3925170772925206 1105 88-89 1.6258350763024438 1105 90-91 0.621672721375436 1105 92-93 0.4223706849511224 1105 94-95 0.37174172570622943 1105 96-97 0.3647350936064697 1105 98-99 0.3059084032796804 1105 100 0.3776675532601068 1106 1 0.23858219614319154 1106 2 0.23377316716685215 1106 3 0.6000089419885768 1106 4 0.27420905691878517 1106 5 0.1857855712617038 1106 6 0.1680780223702527 1106 7 0.14175218391721245 1106 8 0.12619997314570242 1106 9 0.16031475894369862 1106 10-11 0.1812730969491838 1106 12-13 0.14861042791983436 1106 14-15 0.2070421327031582 1106 16-17 0.18266598316566984 1106 18-19 0.20644649137042848 1106 20-21 0.2910842374309155 1106 22-23 0.2590675743227422 1106 24-25 0.23129557615243357 1106 26-27 0.2170543423260085 1106 28-29 0.25264025554669445 1106 30-31 0.2642448161955926 1106 32-33 0.1903300750883261 1106 34-35 0.16815164559433526 1106 36-37 0.20264799166984204 1106 38-39 0.20760874928664208 1106 40-41 0.13671207299293542 1106 42-43 0.15673598894449725 1106 44-45 0.18433566803624757 1106 46-47 0.17271564314690124 1106 48-49 0.21959850949036763 1106 50-51 0.18628420049549277 1106 52-53 0.20864571381986252 1106 54-55 0.2197849880936218 1106 56-57 0.25444431909880905 1106 58-59 0.1954460942190792 1106 60-61 0.20126881207285408 1106 62-63 0.29967333211322256 1106 64-65 0.2983815094988458 1106 66-67 0.280148533730582 1106 68-69 0.24196144549159726 1106 70-71 0.21102868338105907 1106 72-73 0.19415317938143062 1106 74-75 0.21302591623125267 1106 76-77 0.18369346409833298 1106 78-79 0.2670599879639681 1106 80-81 0.3066216418823231 1106 82-83 0.3362322098905324 1106 84-85 0.28840619239557697 1106 86-87 1.3682186553765199 1106 88-89 1.5895455620131997 1106 90-91 0.5360756391716315 1106 92-93 0.34211344879167527 1106 94-95 0.252804788087964 1106 96-97 0.29834866248259573 1106 98-99 0.29630772290035523 1106 100 0.3771827232306322 1107 1 0.24909238312396553 1107 2 0.26002781618365134 1107 3 0.6044856862351438 1107 4 0.288050974213391 1107 5 0.1878791251455425 1107 6 0.16205967300151514 1107 7 0.15674848344036718 1107 8 0.15242313427934562 1107 9 0.19158847244247568 1107 10-11 0.17766318029578798 1107 12-13 0.15446860545320362 1107 14-15 0.2313909840980486 1107 16-17 0.204674972565968 1107 18-19 0.21985460452278716 1107 20-21 0.2874104342046806 1107 22-23 0.2718039025961616 1107 24-25 0.2611505132963785 1107 26-27 0.24105123205179524 1107 28-29 0.2899169648182891 1107 30-31 0.257240063346849 1107 32-33 0.15566269643117892 1107 34-35 0.15453873108628358 1107 36-37 0.13112599751357124 1107 38-39 0.16368002952672356 1107 40-41 0.1367166270256206 1107 42-43 0.18032717941547816 1107 44-45 0.18393738534896187 1107 46-47 0.1716302219847492 1107 48-49 0.2154432799515007 1107 50-51 0.16512850670552837 1107 52-53 0.21964305218293134 1107 54-55 0.26242947063579436 1107 56-57 0.23928420451414212 1107 58-59 0.18901423868236833 1107 60-61 0.18546488738146394 1107 62-63 0.2693596087048675 1107 64-65 0.27151336722149466 1107 66-67 0.18648301103692688 1107 68-69 0.14151431755086463 1107 70-71 0.13288928938488453 1107 72-73 0.14710000285255376 1107 74-75 0.19320247217507358 1107 76-77 0.10564489052546122 1107 78-79 0.21104135234175203 1107 80-81 0.21304303615537634 1107 82-83 0.2333619462146359 1107 84-85 0.2655851539095764 1107 86-87 1.3367126862107064 1107 88-89 1.5899205540761727 1107 90-91 0.5126521672187501 1107 92-93 0.28342575966921046 1107 94-95 0.2554171113524575 1107 96-97 0.25210805550029747 1107 98-99 0.2855948164073965 1107 100 0.36433540667487563 1108 1 0.21221189821930864 1108 2 0.2309385306352425 1108 3 0.5546507606279576 1108 4 0.2627303413726523 1108 5 0.1585902721890804 1108 6 0.12292482655779224 1108 7 0.10351175817335445 1108 8 0.08508388022055868 1108 9 0.11604534320114368 1108 10-11 0.10431530608408224 1108 12-13 0.11653582156532138 1108 14-15 0.1520399738425411 1108 16-17 0.1484113440470054 1108 18-19 0.16860365485788975 1108 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-0.8475494561383812 2316 70-71 -0.82618687877941 2316 72-73 -0.7035731189647692 2316 74-75 -0.7176159967392763 2316 76-77 -0.7232753214265628 2316 78-79 -0.6738318881510494 2316 80-81 -0.6052239851913157 2316 82-83 -0.5748183190130263 2316 84-85 -0.638134004312473 2316 86-87 0.3503140309994244 2316 88-89 0.6811344328351652 2316 90-91 -0.3831156225058834 2316 92-93 -0.7195258465336671 2316 94-95 -0.8551945374398002 2316 96-97 -0.909984455535664 2316 98-99 -0.9235151990477988 2316 100 -0.8766615443268826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 21.0 7 482.0 8 755.0 9 349.0 10 145.0 11 138.0 12 171.0 13 625.0 14 2574.0 15 7794.0 16 16332.0 17 26060.0 18 33231.0 19 43715.0 20 58215.0 21 81570.0 22 121695.0 23 197305.0 24 326794.0 25 425394.0 26 431263.0 27 371311.0 28 336359.0 29 352708.0 30 405513.0 31 489355.0 32 599358.0 33 763639.0 34 1044843.0 35 1611712.0 36 2843857.0 37 5597384.0 38 7105083.0 39 1990716.0 40 4122.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.175740279434915 34.41160985967886 28.19360683074219 21.219043030144032 2 21.094025176480674 33.316730318804765 26.720699415925008 18.868545088789553 3 25.68313556015384 26.058433279605836 26.616447193714908 21.641983966525412 4 19.7756098039318 27.54269295755401 25.849628328135353 26.83206891037883 5 21.193129238434473 32.291997323272994 26.24976532771796 20.265108110574573 6 27.116075751184592 26.665746166123146 26.107854827258265 20.110323255433997 7 20.700194870914032 26.356733975501083 32.13805467868126 20.805016474903628 8 26.596257864783528 26.586475569488538 26.12449738218819 20.692769183539742 9 26.19641346417094 25.869212688926012 27.051150412161235 20.88322343474181 10-11 20.39033453868293 29.690814227015995 29.328916749582888 20.58993448471819 12-13 20.384535938824357 26.693825782144724 29.218553558343523 23.703084720687396 14-15 20.41731097750673 32.80998251207129 26.140659521241655 20.63204698918032 16-17 23.534543759915742 26.56227288981972 26.193799843641436 23.709383506623098 18-19 20.492425877959025 29.63237549083477 26.172080301177658 23.70311833002855 20-21 23.59364084377951 29.653883492151312 26.137415231310555 20.61506043275862 22-23 23.605341564849226 26.66677461397272 29.127150736392405 20.60073308478565 24-25 20.562171191907442 29.654089102238352 25.992993915364877 23.790745790489332 26-27 20.62158459898204 26.589709974319298 32.131643202601694 20.657062224096965 28-29 20.65265149232592 26.67888148745296 32.073710583557805 20.594756436663314 30-31 20.6901397468497 26.6363419466562 32.038640224576824 20.634878081917275 32-33 20.688733744643166 26.727338385911658 31.932571669157845 20.65135620028733 34-35 20.69620405322444 26.803308208172133 31.882489911303935 20.61799782729949 36-37 20.692764566087604 26.7853159844319 31.880247314014596 20.641672135465896 38-39 20.717151606655325 26.78303146566354 31.883104014495217 20.61671291318592 40-41 20.75311070469199 26.818335154403538 31.85282998105437 20.5757241598501 42-43 20.748333332542526 26.877150898982656 31.779634779547237 20.59488098892758 44-45 20.823683498382877 26.93649313333482 31.70785313492909 20.531970233353213 46-47 20.850887294514465 27.049177346133668 31.607228744543225 20.49270661480864 48-49 20.89413315751651 27.099349077606217 31.46996131824339 20.536556446633877 50-51 20.945416214465844 27.151152706040705 31.346057889337224 20.557373190156227 52-53 21.041660602843184 27.30097977677958 31.07666296931621 20.58069665106102 54-55 21.31215770633162 27.557246997898904 30.393111967682074 20.7374833280874 56-57 21.729238852235905 28.065844233326274 29.268771833634144 20.936145080803676 58-59 21.865481797331324 28.588350417029503 28.55327017505474 20.992897610584436 60-61 21.903791872246263 28.871505542333438 28.168809140101995 21.0558934453183 62-63 21.928563926634766 29.016369677723354 27.918753302857546 21.136313092784338 64-65 21.924460855868677 29.168477404395272 27.806199629882933 21.100862109853118 66-67 21.83928037021819 29.0948860756931 27.864774299957656 21.201059254131057 68-69 21.772757269629807 28.989964211844054 27.98442616177069 21.252852356755444 70-71 21.713043147839667 28.950701790825672 28.04644769364702 21.289807367687636 72-73 21.67492258286841 28.900875426973048 28.011361827793614 21.412840162364922 74-75 21.66984690131408 28.97585860156972 27.84485946049109 21.509435036625106 76-77 21.681417355366424 29.11585700139261 27.618692236921905 21.584033406319058 78-79 21.754395304861234 29.28486331297314 27.240982230194078 21.719759151971548 80-81 21.82111222666477 29.475029579002758 26.88501811917231 21.81884007516016 82-83 21.7859173580862 29.644566297064735 26.58020286297847 21.9893134818706 84-85 21.76043508355582 29.70355581924256 26.402464155047955 22.133544942153662 86-87 22.550687435143153 29.447098495641256 26.00795940466451 21.99425466455108 88-89 21.7572625644478 29.79759968247519 26.084483149782837 22.360654603294172 90-91 21.7561173891034 29.815716430364237 25.939072199322215 22.489093981210143 92-93 21.75356756482003 29.85002589216873 25.833611089523046 22.562795453488192 94-95 21.763271817236728 29.868112969498277 25.744941540913295 22.6236736723517 96-97 21.76236356235464 29.778913547365832 25.688569319199694 22.770153571079838 98-99 21.748546706601445 29.710116317840335 25.67711407519117 22.864222900367047 100 21.669724157578713 29.735402976906798 25.630413503651294 22.96445936186319 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 48396.0 1 31419.0 2 11069.0 3 7066.5 4 6293.0 5 6231.0 6 6551.0 7 6731.5 8 6547.0 9 6698.5 10 7429.0 11 11083.5 12 106901.5 13 131317.5 14 59112.5 15 58567.0 16 65739.0 17 72757.0 18 79450.0 19 87025.0 20 97046.5 21 112006.5 22 131971.5 23 154799.0 24 179656.5 25 208619.5 26 241657.0 27 280088.5 28 324760.0 29 368094.5 30 404263.0 31 441004.5 32 481955.5 33 516456.0 34 547859.0 35 586405.5 36 627009.0 37 661723.5 38 700417.5 39 733696.0 40 757948.0 41 793094.0 42 831804.5 43 843241.0 44 839463.5 45 849796.5 46 858184.5 47 858809.5 48 859517.0 49 858968.0 50 856764.0 51 851386.0 52 834503.5 53 795415.0 54 743008.5 55 689368.0 56 632329.5 57 569455.0 58 498633.0 59 419479.0 60 339635.5 61 271544.0 62 213177.0 63 160063.0 64 117805.5 65 86890.5 66 64147.0 67 48282.5 68 36684.0 69 28154.5 70 21679.5 71 17142.5 72 13955.5 73 10798.0 74 8358.0 75 6850.0 76 5029.5 77 3020.0 78 1685.5 79 1028.5 80 625.5 81 411.0 82 270.0 83 168.5 84 107.0 85 76.0 86 51.0 87 34.0 88 21.0 89 13.5 90 11.5 91 7.5 92 3.5 93 4.0 94 3.5 95 2.5 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17961227315078637 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0018623529037759027 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 7.908080270810628E-6 34-35 0.001589524134432936 36-37 5.891519801753917E-4 38-39 1.423454448745913E-4 40-41 2.2340326765040022E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0024910452853053474 50-51 0.0069927199794642975 52-53 0.015863609023246118 54-55 0.023413848661802565 56-57 0.017737824047428238 58-59 0.028172535964762862 60-61 0.028445364734105825 62-63 0.00743359545456199 64-65 0.0019038703251976583 66-67 0.002089710211561708 68-69 3.8749593326972074E-4 70-71 2.965530101553985E-4 72-73 0.002542447807065617 74-75 0.005185723637584069 76-77 0.01816288336198431 78-79 0.030671489330339016 80-81 0.024445853137143352 82-83 0.026391240883762764 84-85 0.031980276615158176 86-87 1.4699717539188888 88-89 0.004845676185939212 90-91 3.183002309001277E-4 92-93 4.843699165871509E-4 94-95 3.163232108324251E-4 96-97 2.0363306697337364E-4 98-99 7.334744451176857E-4 100 9.608317529034913E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.5290588E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.41866408712774 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90647407781765 37.237087344992496 2 12.262827427646155 11.874994439552317 3 4.089398239907855 5.940095990897693 4 1.9435159743571981 3.764097884414718 5 1.145667635258944 2.77358481935484 6 0.7185475853099397 2.0874668498243243 7 0.5143690808779602 1.7433544620684214 8 0.3922526028624136 1.5193877612237379 9 0.28905226545757234 1.2595972090332126 >10 1.503256653094304 13.302918507999568 >50 0.13666788244510183 4.760005759601429 >100 0.09718536016600553 7.526728940817868 >500 1.9782649771984297E-4 0.06658493622652543 >1k 5.466977460677089E-4 0.4848645675749629 >5k 8.138111058425738E-6 0.020958799607479647 >10k+ 3.2552444231274314E-5 5.638271726810448 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1344363 5.315665258553894 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38988 0.15416011679918237 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26152 0.10340605762111975 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.019572498670256303 3.954040135405314E-6 0.0 0.0 0.0 2 0.019584360790662517 1.186212040621594E-5 0.0 0.0 7.908080270810628E-6 3 0.019623901192016572 1.977020067702657E-5 0.0 0.0 1.5816160541621256E-5 4 0.019663441593370624 4.744848162486376E-5 0.0 0.0 1.977020067702657E-5 5 0.019679257753912247 5.140252176026908E-5 0.0 0.0 2.372424081243188E-5 6 0.0197187981552663 5.535656189567439E-5 0.0 0.0 2.372424081243188E-5 7 0.019746476436214137 6.721868230189033E-5 0.0 0.0 2.7678280947837194E-5 8 0.01978601683756819 7.908080270810628E-5 0.0 0.0 3.163232108324251E-5 9 0.01980974107838062 9.094292311432222E-5 0.0 0.0 3.163232108324251E-5 10-11 0.019839396379396163 1.1862120406215941E-4 0.0 7.908080270810628E-6 4.349444148945845E-5 12-13 0.019894752941291834 1.4036842480688865E-4 0.0 7.908080270810628E-6 4.942550169256642E-5 14-15 0.019977787784135347 1.6804670575472583E-4 0.0 1.186212040621594E-5 5.140252176026908E-5 16-17 0.020046983486504938 1.7595478602553646E-4 0.0 1.186212040621594E-5 5.140252176026908E-5 18-19 0.020141880449754665 1.9374796663486037E-4 0.0 1.186212040621594E-5 5.733358196337705E-5 20-21 0.02021898423239507 2.3131134792121085E-4 0.0 1.186212040621594E-5 5.9310602031079705E-5 22-23 0.020369237757540473 2.530585686659401E-4 0.0 1.3839140473918597E-5 6.721868230189033E-5 24-25 0.02097222887818978 2.7282876934296664E-4 0.0 1.5816160541621256E-5 6.721868230189033E-5 26-27 0.02290377748433528 2.827138696814799E-4 0.0 2.1747220744729225E-5 6.721868230189033E-5 28-29 0.02932513866423351 3.123691706970198E-4 1.977020067702657E-6 2.372424081243188E-5 7.31497425049983E-5 30-31 0.045311322931677195 3.34116391441749E-4 3.954040135405314E-6 2.5701260880134535E-5 7.710378264040363E-5 32-33 0.07834139720278549 3.6772573259269414E-4 3.954040135405314E-6 3.163232108324251E-5 8.30348428435116E-5 34-35 0.13765793029406828 3.8156487306661275E-4 3.954040135405314E-6 3.558636121864782E-5 8.896590304661956E-5 36-37 0.23109189869369584 3.914499734051261E-4 3.954040135405314E-6 3.954040135405314E-5 9.094292311432222E-5 38-39 0.3636669103937006 3.993580536759367E-4 3.954040135405314E-6 3.954040135405314E-5 9.489696324972753E-5 40-41 0.5418082806141162 4.052891138790446E-4 3.954040135405314E-6 3.954040135405314E-5 1.0280504352053816E-4 42-43 0.7675147766433901 4.2110527442066595E-4 3.954040135405314E-6 3.954040135405314E-5 1.0280504352053816E-4 44-45 1.041678034532056 4.388984550299898E-4 3.954040135405314E-6 4.349444148945845E-5 1.0478206358824081E-4 46-47 1.3698851129914418 4.6459971591012437E-4 3.954040135405314E-6 4.54714615571611E-5 1.0873610372364612E-4 48-49 1.7530750965537059 4.843699165871509E-4 3.954040135405314E-6 5.3379541827971736E-5 1.1071312379134878E-4 50-51 2.1862500784876966 4.942550169256642E-4 3.954040135405314E-6 5.9310602031079705E-5 1.1269014385905144E-4 52-53 2.665525214360378 5.061171373318802E-4 3.954040135405314E-6 5.9310602031079705E-5 1.1862120406215941E-4 54-55 3.1940775754205477 5.29841378144312E-4 3.954040135405314E-6 6.128762209878236E-5 1.1862120406215941E-4 56-57 3.769914325439962 5.397264784828253E-4 3.954040135405314E-6 6.326464216648502E-5 1.1862120406215941E-4 58-59 4.390297291624853 5.47634558753636E-4 3.954040135405314E-6 6.721868230189033E-5 1.2059822412986206E-4 60-61 5.051762734816604 5.555426390244467E-4 3.954040135405314E-6 6.721868230189033E-5 1.2257524419756471E-4 62-63 5.748037570340397 5.634507192952572E-4 3.954040135405314E-6 6.721868230189033E-5 1.285063044006727E-4 64-65 6.474960170953716 5.772898597691758E-4 3.954040135405314E-6 7.117272243729564E-5 1.32460344536078E-4 66-67 7.233554633051632 5.93106020310797E-4 3.954040135405314E-6 7.908080270810628E-5 1.3443736460378065E-4 68-69 8.021501912094728 5.93106020310797E-4 3.954040135405314E-6 9.291994318202487E-5 1.3443736460378065E-4 70-71 8.834138217743297 6.069451607847156E-4 3.954040135405314E-6 9.489696324972752E-5 1.423454448745913E-4 72-73 9.662578821813078 6.385774818679582E-4 3.954040135405314E-6 9.489696324972752E-5 1.5025352514540192E-4 74-75 10.512505679978656 6.563706624772821E-4 3.954040135405314E-6 9.489696324972752E-5 1.5025352514540192E-4 76-77 11.340309683586637 6.662557628157953E-4 3.954040135405314E-6 9.489696324972752E-5 1.5025352514540192E-4 78-79 12.16899148410468 6.860259634928219E-4 5.931060203107971E-6 9.489696324972752E-5 1.561845853485099E-4 80-81 13.001613485617654 7.137042444406591E-4 7.908080270810628E-6 9.489696324972752E-5 1.6211564555161786E-4 82-83 13.852570371238501 7.275433849145777E-4 7.908080270810628E-6 9.885100338513284E-5 1.6804670575472583E-4 84-85 14.705757335495719 7.473135855916043E-4 7.908080270810628E-6 1.0478206358824081E-4 1.739777659578338E-4 86-87 15.57801068128586 7.532446457947123E-4 7.908080270810628E-6 1.1269014385905144E-4 1.8188584622864443E-4 88 16.26335061881519 7.670837862686308E-4 7.908080270810628E-6 1.1862120406215941E-4 1.8188584622864443E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 168265 0.0 86.043495 8 GTGGTAT 170235 0.0 85.34525 6 AGTGGTA 170575 0.0 85.24112 5 TGGTATC 170265 0.0 84.99699 7 GTATCAA 170305 0.0 84.844864 9 GCAGTGG 176215 0.0 82.23612 3 AAGCAGT 176030 0.0 82.17574 1 CAGTGGT 178370 0.0 81.46603 4 AGCAGTG 184190 0.0 78.60421 2 CAACGCA 164370 0.0 44.814316 12-13 ATCAACG 165680 0.0 44.58309 10-11 ACGCAGA 166360 0.0 44.382538 14-15 TCAACGC 163965 0.0 44.025593 12-13 AACGCAG 165215 0.0 43.672634 14-15 AGAGTAC 168850 0.0 43.48928 18-19 GAGTACT 164660 0.0 43.26028 20-21 CGCAGAG 167620 0.0 43.116684 16-17 TATCAAC 170020 0.0 42.470104 10-11 AGTACTT 171700 0.0 42.442413 20-21 GTACTTT 171900 0.0 41.390526 22-23 >>END_MODULE