FastQCFastQC Report
Thu 26 May 2016
SRR1660774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32733513
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15020674.5887742021456726No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA433200.1323414324640316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1926750.083.737458
GTGGTAT1945000.083.0691156
AGTGGTA1947950.082.989035
TGGTATC1947050.082.5631567
GTATCAA1957100.082.356239
AAGCAGT2012850.080.729061
GCAGTGG2025000.080.469353
CAGTGGT2060550.078.46224
AGCAGTG2110000.077.251782
CAACGCA1898600.043.55731212-13
ATCAACG1919200.043.21170810-11
ACGCAGA1923150.043.07290614-15
GAGTACT1850650.042.8714320-21
TCAACGC1895950.042.69848312-13
AACGCAG1908350.042.49930614-15
AGAGTAC1929200.042.42525518-19
CGCAGAG1942350.041.66375716-17
AGTACTT1955350.041.4934820-21
TATCAAC1968900.040.9309510-11
GTACTTT1954500.040.6333122-23
Produced by FastQC (version 0.11.5)