FastQCFastQC Report
Thu 26 May 2016
SRR1660774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32733513
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15053294.598739524230107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA405050.12374168333230838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1897100.083.665158
AGTGGTA1919100.083.024375
GTGGTAT1921600.082.899246
TGGTATC1921400.082.558137
GTATCAA1927300.082.237129
GCAGTGG1986950.080.251793
AAGCAGT1990000.080.093021
CAGTGGT2036750.078.1326754
AGCAGTG2084650.076.483922
CAACGCA1864900.043.39019412-13
ATCAACG1877850.043.1522810-11
ACGCAGA1895700.042.869914-15
GAGTACT1826400.042.7942520-21
TCAACGC1858000.042.5447212-13
AACGCAG1875000.042.28679714-15
AGAGTAC1916150.042.2024918-19
AGTACTT1922850.041.61429620-21
CGCAGAG1914450.041.43984616-17
TATCAAC1938000.040.83334410-11
GTACTTT1923750.040.618922-23
Produced by FastQC (version 0.11.5)