FastQCFastQC Report
Thu 26 May 2016
SRR1660773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32580358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12367613.7960325666157506No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA373350.11459358426939323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1572150.084.0317468
GTGGTAT1591950.083.084246
TGGTATC1589900.082.831127
AGTGGTA1599150.082.796165
GTATCAA1603450.082.31329
GCAGTGG1700800.078.3146443
AAGCAGT1708100.077.6539841
CAGTGGT1717150.077.069334
AGCAGTG1801000.073.907822
CAACGCA1540950.043.9529112-13
ATCAACG1553650.043.65987810-11
ACGCAGA1571700.043.1761114-15
TCAACGC1533400.043.1516812-13
AACGCAG1552750.042.72829414-15
GAGTACT1529800.042.55430220-21
AGAGTAC1585400.042.38501718-19
CGCAGAG1584750.041.8299716-17
AGTACTT1622300.041.05276520-21
TATCAAC1613650.040.8655710-11
GTACTTT1624450.040.08734522-23
Produced by FastQC (version 0.11.5)