FastQCFastQC Report
Thu 26 May 2016
SRR1660773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32580358
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13128144.029464624053547No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA343130.10531805697162688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1652150.084.32248
GTGGTAT1680450.083.279886
AGTGGTA1682450.083.2478945
TGGTATC1680750.082.887557
GTATCAA1687600.082.5093469
GCAGTGG1775200.078.9398353
AAGCAGT1780300.078.770311
CAGTGGT1807450.077.450724
AGCAGTG1876200.074.795532
CAACGCA1617100.044.03690312-13
ATCAACG1630250.043.71916210-11
ACGCAGA1653750.043.25419214-15
TCAACGC1611100.043.14648412-13
AACGCAG1634050.042.6972314-15
GAGTACT1612900.042.59625220-21
AGAGTAC1676200.042.43292218-19
CGCAGAG1670650.041.8331216-17
AGTACTT1703050.041.29482720-21
TATCAAC1693850.041.08857710-11
GTACTTT1705250.040.2480622-23
Produced by FastQC (version 0.11.5)