FastQCFastQC Report
Thu 26 May 2016
SRR1660772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38016662
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19629305.163341273886697No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1640740.4315844457885335Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA611790.16092680625142733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2473600.085.256738
AGTGGTA2490300.084.749885
GTATCAA2495550.084.403469
TGGTATC2502500.083.980247
GTGGTAT2516750.083.883026
AAGCAGT2582850.081.8938451
GCAGTGG2603650.081.5995943
CAGTGGT2624250.080.363964
AGCAGTG2721100.078.093482
CAACGCA2437050.044.23500412-13
ATCAACG2458800.043.98705310-11
ACGCAGA2474550.043.65570414-15
TCAACGC2432850.043.35022412-13
GAGTACT2393550.043.21882220-21
AGAGTAC2479500.043.0845518-19
AACGCAG2457800.042.99411814-15
CGCAGAG2498150.042.27538316-17
AGTACTT2504200.042.20131320-21
GTACTTT2488950.041.54569222-23
TATCAAC2539200.041.44664810-11
Produced by FastQC (version 0.11.5)