##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660772_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38016662 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.005790487339475 34.0 31.0 34.0 30.0 34.0 2 32.20935104718031 34.0 31.0 34.0 30.0 34.0 3 32.251708369346055 34.0 31.0 34.0 30.0 34.0 4 35.6888270727188 37.0 35.0 37.0 35.0 37.0 5 35.68892936996941 37.0 35.0 37.0 35.0 37.0 6 35.73056156271689 37.0 35.0 37.0 35.0 37.0 7 35.62454599512182 37.0 35.0 37.0 33.0 37.0 8 35.684034358408425 37.0 35.0 37.0 33.0 37.0 9 37.391515830611326 39.0 37.0 39.0 35.0 39.0 10-11 37.2749811779898 39.0 37.0 39.0 34.0 39.0 12-13 37.370093605272345 39.0 37.0 39.0 34.0 39.0 14-15 38.79099444606683 40.5 38.0 41.0 35.0 41.0 16-17 38.760697151685754 40.0 38.0 41.0 35.0 41.0 18-19 38.70792685586126 40.0 38.5 41.0 34.0 41.0 20-21 38.62548288695099 40.0 38.5 41.0 34.0 41.0 22-23 38.464338702330046 40.0 38.0 41.0 34.0 41.0 24-25 38.494613361899056 40.0 38.0 41.0 34.0 41.0 26-27 38.50856652012215 40.0 38.0 41.0 34.0 41.0 28-29 38.45889145922385 40.0 38.0 41.0 34.0 41.0 30-31 38.34933693021234 40.0 38.0 41.0 33.5 41.0 32-33 38.23916187065556 40.0 38.0 41.0 33.0 41.0 34-35 38.15096045255105 40.0 38.0 41.0 33.0 41.0 36-37 37.99437069198763 40.0 38.0 41.0 33.0 41.0 38-39 37.61452721703973 40.0 36.5 41.0 33.0 41.0 40-41 37.49618974964188 40.0 36.0 41.0 32.5 41.0 42-43 37.33194929107663 40.0 36.0 41.0 32.0 41.0 44-45 37.210229306823415 40.0 35.5 41.0 31.5 41.0 46-47 37.047755731421134 40.0 35.0 41.0 31.0 41.0 48-49 36.90612967282609 39.0 35.0 41.0 31.0 41.0 50-51 35.594708867390835 38.0 34.0 39.5 29.0 40.0 52-53 35.63210532529131 38.5 34.5 39.5 28.5 40.0 54-55 36.19219479343031 39.0 35.0 40.5 28.5 41.0 56-57 36.01007977765118 39.0 35.0 41.0 27.0 41.0 58-59 35.87921305400248 39.0 35.0 41.0 26.5 41.0 60-61 35.679028356040305 39.0 35.0 41.0 26.0 41.0 62-63 35.421634308661815 38.5 35.0 41.0 26.0 41.0 64-65 35.10937509453092 37.5 35.0 40.5 26.0 41.0 66-67 34.76659788016107 37.0 34.0 40.0 25.0 41.0 68-69 34.453146307269165 36.5 34.0 40.0 25.0 41.0 70-71 34.139992485400214 36.0 34.0 39.0 25.0 41.0 72-73 33.795268466758074 35.5 34.0 39.0 24.0 41.0 74-75 33.43529921169828 35.0 33.5 38.0 24.0 40.0 76-77 33.07368846586268 35.0 33.0 37.0 23.5 39.5 78-79 32.72612996638158 35.0 33.0 37.0 23.0 39.0 80-81 32.327704810064596 35.0 33.0 36.0 20.5 39.0 82-83 32.059670849060865 35.0 33.0 36.0 20.0 37.0 84-85 31.807626416543357 35.0 32.5 35.5 20.0 37.0 86-87 31.600393388036014 35.0 32.0 35.0 20.0 36.5 88-89 31.425839530572148 35.0 32.0 35.0 19.5 36.0 90-91 31.25860763367389 35.0 32.0 35.0 19.0 36.0 92-93 31.11684366186595 35.0 32.0 35.0 19.0 36.0 94-95 30.984426670600378 35.0 32.0 35.0 18.0 35.5 96-97 30.87494989696886 35.0 31.0 35.0 18.0 35.0 98-99 30.765621150536575 35.0 31.0 35.0 17.0 35.0 100 30.685012561071247 35.0 31.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4741098678343292 1101 2 0.4539260801703051 1101 3 0.4219046761723817 1101 4 0.32176610805403527 1101 5 0.30901211086276703 1101 6 0.2855791537772703 1101 7 0.3478391944821837 1101 8 0.32590280794828175 1101 9 0.40352323901715437 1101 10-11 0.3707894137540464 1101 12-13 0.3631464617813549 1101 14-15 0.427181852850552 1101 16-17 0.4321773567049547 1101 18-19 0.45453023140932913 1101 20-21 0.4476185932185217 1101 22-23 0.44576157807728123 1101 24-25 0.4382648394477471 1101 26-27 0.39066174509022034 1101 28-29 0.35624069737679775 1101 30-31 0.3826753581711557 1101 32-33 0.3843485503697366 1101 34-35 0.3935737223340112 1101 36-37 0.3526217290697673 1101 38-39 0.3650490898840246 1101 40-41 0.33628851338546184 1101 42-43 0.34297987240459804 1101 44-45 0.302361880245563 1101 46-47 0.26864761997835274 1101 48-49 0.2607651236776505 1101 50-51 0.20215773892343947 1101 52-53 0.34418441512788434 1101 54-55 0.49557844067687995 1101 56-57 0.4373160970893366 1101 58-59 0.32960357508377314 1101 60-61 0.34902278189203173 1101 62-63 0.3706015078722231 1101 64-65 0.2925214559049607 1101 66-67 0.2639798865997989 1101 68-69 0.2706298453778331 1101 70-71 0.24242012138670788 1101 72-73 0.24377876888492978 1101 74-75 0.16805052789944597 1101 76-77 0.18064713244947228 1101 78-79 0.15563215097129302 1101 80-81 0.24479027784121854 1101 82-83 0.18114738633762784 1101 84-85 0.14697648547209496 1101 86-87 0.1432396583034503 1101 88-89 0.16194340662189788 1101 90-91 0.10814444275748869 1101 92-93 0.09356078007022717 1101 94-95 0.061854998520750826 1101 96-97 -0.08565313663815743 1101 98-99 -0.011365452723747893 1101 100 -0.02145033743592606 1102 1 0.4631998047270187 1102 2 0.4343709728449525 1102 3 0.39797705023077157 1102 4 0.32404193617195887 1102 5 0.3194275043793269 1102 6 0.32162238085973627 1102 7 0.3574476818338539 1102 8 0.31963531364569775 1102 9 0.3884727354512165 1102 10-11 0.38109696384726277 1102 12-13 0.39025108708782597 1102 14-15 0.4443628713962724 1102 16-17 0.45670280703788535 1102 18-19 0.4468105287628674 1102 20-21 0.4347118025393968 1102 22-23 0.40831698315108866 1102 24-25 0.41581293890884297 1102 26-27 0.38148251762492436 1102 28-29 0.3779087314819449 1102 30-31 0.3787378498121896 1102 32-33 0.3789283372202732 1102 34-35 0.35836922224343937 1102 36-37 0.34498368719815176 1102 38-39 0.34612339982243157 1102 40-41 0.37781201327376834 1102 42-43 0.33697039514462546 1102 44-45 0.2933553735040064 1102 46-47 0.30463103473771014 1102 48-49 0.2602460532578519 1102 50-51 0.33144268396991805 1102 52-53 0.39043793219416756 1102 54-55 0.45359055447885765 1102 56-57 0.42093456879025837 1102 58-59 0.415730992879908 1102 60-61 0.40385784074291564 1102 62-63 0.4360798872781686 1102 64-65 0.36672214287131766 1102 66-67 0.34581128672363093 1102 68-69 0.3623034556496947 1102 70-71 0.32231889711152206 1102 72-73 0.27913232016071987 1102 74-75 0.28957590884648 1102 76-77 0.27829117294322003 1102 78-79 0.23821568951559158 1102 80-81 0.2868334366495091 1102 82-83 0.2042286811918359 1102 84-85 0.17691753631658003 1102 86-87 0.2146751445727375 1102 88-89 0.21721860190324094 1102 90-91 0.18259895128032966 1102 92-93 0.17023802726301795 1102 94-95 0.11320664676850001 1102 96-97 0.1635077396167901 1102 98-99 0.1492223198396765 1102 100 0.11901496670968825 1103 1 0.46237704260297363 1103 2 0.41916502960736324 1103 3 0.41635166635845167 1103 4 0.3485823462165314 1103 5 0.30891660031334567 1103 6 0.31855631556859265 1103 7 0.36697249834418244 1103 8 0.34362564968967746 1103 9 0.39502597801744344 1103 10-11 0.37191345259805075 1103 12-13 0.38120620491191204 1103 14-15 0.42620672519120006 1103 16-17 0.46450485450088763 1103 18-19 0.46254593994509463 1103 20-21 0.42181351428412484 1103 22-23 0.4228002240781237 1103 24-25 0.3939165734320724 1103 26-27 0.40403541904335327 1103 28-29 0.35444178106887847 1103 30-31 0.3717839550616304 1103 32-33 0.38410528811203903 1103 34-35 0.38087134004745593 1103 36-37 0.3489310420135183 1103 38-39 0.352781041627793 1103 40-41 0.3500863414573345 1103 42-43 0.3508636394525695 1103 44-45 0.3077985109421988 1103 46-47 0.2761405441470899 1103 48-49 0.29051201539342486 1103 50-51 0.2264844955134464 1103 52-53 0.3634875057664999 1103 54-55 0.39931527368874953 1103 56-57 0.4051478489333462 1103 58-59 0.35217536207925804 1103 60-61 0.34890133740326235 1103 62-63 0.3408183387445902 1103 64-65 0.306414749873106 1103 66-67 0.2580345663737802 1103 68-69 0.2361558678197042 1103 70-71 0.29883276517926305 1103 72-73 0.26625737783020753 1103 74-75 0.2637526156900307 1103 76-77 0.2627449750525628 1103 78-79 0.25316470326301754 1103 80-81 0.2549177395457818 1103 82-83 0.27560634587207744 1103 84-85 0.2383842891958352 1103 86-87 0.2502913957408204 1103 88-89 0.22935994631327716 1103 90-91 0.20800319515476673 1103 92-93 0.19623416713889696 1103 94-95 0.19350997423043736 1103 96-97 0.20815648261105224 1103 98-99 0.17599894059382848 1103 100 0.20412146358164662 1104 1 0.499044684771512 1104 2 0.4661551679142306 1104 3 0.42899110726278167 1104 4 0.3727158121433334 1104 5 0.34095547453369335 1104 6 0.3335264416660664 1104 7 0.36219335511414386 1104 8 0.3623128684087078 1104 9 0.4242406279604367 1104 10-11 0.42697362686929097 1104 12-13 0.4023055991968789 1104 14-15 0.48875791689280135 1104 16-17 0.4720584079237824 1104 18-19 0.45587201510062414 1104 20-21 0.44440485334612134 1104 22-23 0.47385930103332896 1104 24-25 0.4395624109616776 1104 26-27 0.4518944214639973 1104 28-29 0.4423231804741974 1104 30-31 0.43838034109267454 1104 32-33 0.4353990823788578 1104 34-35 0.4207958771459772 1104 36-37 0.416599173991969 1104 38-39 0.38931093657922133 1104 40-41 0.4154250966441779 1104 42-43 0.4228620582510274 1104 44-45 0.3837810905529224 1104 46-47 0.36339080170099436 1104 48-49 0.41286952762332163 1104 50-51 0.3443444754667624 1104 52-53 0.44427029863793166 1104 54-55 0.5024076064907987 1104 56-57 0.5083067682207201 1104 58-59 0.4421402948838775 1104 60-61 0.41480511668135733 1104 62-63 0.4547149139589024 1104 64-65 0.43083141638999933 1104 66-67 0.3993602410552626 1104 68-69 0.36777452670187927 1104 70-71 0.3796724315093343 1104 72-73 0.3541499883156476 1104 74-75 0.32372353784887764 1104 76-77 0.3503827169756306 1104 78-79 0.312133151057175 1104 80-81 0.30278092266063794 1104 82-83 0.33662158667860353 1104 84-85 0.2986078070422806 1104 86-87 0.31000512143397785 1104 88-89 0.29453875469353363 1104 90-91 0.3430875163965261 1104 92-93 0.3334069561684885 1104 94-95 0.31826909409207715 1104 96-97 0.3060039706559401 1104 98-99 0.321094929819548 1104 100 0.31584163099694607 1105 1 0.3481747713712977 1105 2 0.3365456032178429 1105 3 0.3031418126848564 1105 4 0.260168076797747 1105 5 0.25250432683431256 1105 6 0.2508536416087779 1105 7 0.27467494153061267 1105 8 0.27815695769002247 1105 9 0.3012183826865069 1105 10-11 0.32256480110948615 1105 12-13 0.29212301283796904 1105 14-15 0.3557584199305879 1105 16-17 0.33990877000010755 1105 18-19 0.36879378336599444 1105 20-21 0.3552864985616395 1105 22-23 0.35521005839676434 1105 24-25 0.335517805929868 1105 26-27 0.28830308809531147 1105 28-29 0.314543171232053 1105 30-31 0.31354892681565616 1105 32-33 0.3272100570155345 1105 34-35 0.3219538754174991 1105 36-37 0.3327653612422026 1105 38-39 0.30428008491848146 1105 40-41 0.3592926881077503 1105 42-43 0.35914088476064165 1105 44-45 0.32528186846565177 1105 46-47 0.285857461290405 1105 48-49 0.2917686939342019 1105 50-51 0.20133673088415804 1105 52-53 0.28859999305743855 1105 54-55 0.42893640049590687 1105 56-57 0.44484441684721787 1105 58-59 0.42692997914564756 1105 60-61 0.43246520567109314 1105 62-63 0.42591566358088784 1105 64-65 0.3977912837525466 1105 66-67 0.3880906201938501 1105 68-69 0.3757316355993794 1105 70-71 0.3632962841219154 1105 72-73 0.3489910415124271 1105 74-75 0.3247793682915656 1105 76-77 0.34001437367247433 1105 78-79 0.33947048000319313 1105 80-81 0.3075759529224982 1105 82-83 0.33490799952059547 1105 84-85 0.2876936801830823 1105 86-87 0.2945929117647985 1105 88-89 0.3027398711325908 1105 90-91 0.32466550758127966 1105 92-93 0.3088030977389451 1105 94-95 0.2979379587153197 1105 96-97 0.2785190476093966 1105 98-99 0.2705155027640913 1105 100 0.22357071610271362 1106 1 0.29939889179352974 1106 2 0.2556297305385371 1106 3 0.23492573199251154 1106 4 0.19577920681446415 1106 5 0.184997033089509 1106 6 0.19844241113876393 1106 7 0.2498141144048347 1106 8 0.19629441962283067 1106 9 0.240240832148352 1106 10-11 0.22210670203396177 1106 12-13 0.21071014335772276 1106 14-15 0.22961790385951275 1106 16-17 0.2427586242022386 1106 18-19 0.22261751066072577 1106 20-21 0.22977277318699407 1106 22-23 0.2601824000388646 1106 24-25 0.23271302667578198 1106 26-27 0.20685420728479897 1106 28-29 0.218610901228395 1106 30-31 0.21551550077101922 1106 32-33 0.23299033412101977 1106 34-35 0.20435377648563957 1106 36-37 0.18496203794457244 1106 38-39 0.22440477574439655 1106 40-41 0.21671154453484576 1106 42-43 0.18502749435227628 1106 44-45 0.2092271247919868 1106 46-47 0.14569430557797602 1106 48-49 0.1453556790509012 1106 50-51 0.09574619032565579 1106 52-53 0.1802836916220656 1106 54-55 0.2610799877491914 1106 56-57 0.2655447448996284 1106 58-59 0.22126953678910155 1106 60-61 0.26056286857210864 1106 62-63 0.2608175131463142 1106 64-65 0.23790033941649824 1106 66-67 0.2501820063048399 1106 68-69 0.2068390599565646 1106 70-71 0.24250840609563085 1106 72-73 0.25782123442858307 1106 74-75 0.209045871490531 1106 76-77 0.24662609387154077 1106 78-79 0.25633802436508546 1106 80-81 0.290124882495995 1106 82-83 0.2884270038928918 1106 84-85 0.21877731043716153 1106 86-87 0.2537997298971568 1106 88-89 0.24183875108609598 1106 90-91 0.2725988606932823 1106 92-93 0.2108923808017238 1106 94-95 0.23795895886496155 1106 96-97 0.2266373587076167 1106 98-99 0.24295854424556396 1106 100 0.24929296005515056 1107 1 0.28247101653806084 1107 2 0.24121234533376423 1107 3 0.2168911889211813 1107 4 0.1599968032441268 1107 5 0.18215695022746559 1107 6 0.17600330754036975 1107 7 0.2155147478127759 1107 8 0.18200895985937393 1107 9 0.22740403791476638 1107 10-11 0.18964718572441086 1107 12-13 0.1834572638882861 1107 14-15 0.20874636000147007 1107 16-17 0.19498691601010876 1107 18-19 0.18425497069829078 1107 20-21 0.15618589481816514 1107 22-23 0.1907164050988044 1107 24-25 0.18018429966085137 1107 26-27 0.17544015661518841 1107 28-29 0.2064581294585608 1107 30-31 0.18162286223407165 1107 32-33 0.176735249597904 1107 34-35 0.16325053256710476 1107 36-37 0.14881130834704948 1107 38-39 0.14890412910335726 1107 40-41 0.18620559762540978 1107 42-43 0.1987431644487998 1107 44-45 0.18942887537112796 1107 46-47 0.1882911574524968 1107 48-49 0.21515862728487178 1107 50-51 0.15830093095765818 1107 52-53 0.19490671073079113 1107 54-55 0.26885140242321626 1107 56-57 0.28265126562300225 1107 58-59 0.244478832541013 1107 60-61 0.19890406786637982 1107 62-63 0.2154412894362352 1107 64-65 0.21853518917055936 1107 66-67 0.24773137340156381 1107 68-69 0.22870240349354987 1107 70-71 0.2366620606367249 1107 72-73 0.25038999030254416 1107 74-75 0.2556086266178781 1107 76-77 0.2572430346050041 1107 78-79 0.22988294280147414 1107 80-81 0.21371499208738953 1107 82-83 0.2630921823677568 1107 84-85 0.22784135156494756 1107 86-87 0.23707109684407257 1107 88-89 0.26999050386053725 1107 90-91 0.297246602462085 1107 92-93 0.314541704900801 1107 94-95 0.2962999347538293 1107 96-97 0.29583722049726546 1107 98-99 0.32477286728411414 1107 100 0.2814204458300189 1108 1 0.23508058908468143 1108 2 0.18938878642503454 1108 3 0.14962121610156487 1108 4 0.15093254679166535 1108 5 0.12190618882119253 1108 6 0.14762124248554187 1108 7 0.15770839786291901 1108 8 0.15850193686597436 1108 9 0.13003847333506258 1108 10-11 0.13453206086707326 1108 12-13 0.1256668651105386 1108 14-15 0.16804045289064362 1108 16-17 0.17325529547069607 1108 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-0.5745839522152387 2316 88-89 -0.6470458502864922 2316 90-91 -0.6359677925023668 2316 92-93 -0.6014689553000814 2316 94-95 -0.5857451463626226 2316 96-97 -0.5537976269619094 2316 98-99 -0.49588232922942765 2316 100 -0.5298500773612638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 13.0 6 925.0 7 9996.0 8 20079.0 9 26167.0 10 30885.0 11 31266.0 12 32177.0 13 36845.0 14 45045.0 15 59519.0 16 82210.0 17 109236.0 18 118408.0 19 132612.0 20 153747.0 21 191950.0 22 254045.0 23 381146.0 24 558801.0 25 658632.0 26 660730.0 27 585920.0 28 489882.0 29 498530.0 30 567122.0 31 689382.0 32 860887.0 33 1119994.0 34 1544619.0 35 2383645.0 36 4212625.0 37 8199479.0 38 1.022855E7 39 3034273.0 40 7320.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.115900141614116 34.04949724367512 27.341092170231757 21.49351044447901 2 21.846055950993993 32.672828895498775 26.283872077323238 19.197243076184 3 25.91611496813659 25.858189156617716 26.28307146922485 21.94262440602084 4 20.109235267589426 26.95585517641704 25.525582210632408 27.409327345361127 5 21.52676973313343 31.712703675601304 25.796038539586526 20.964488051678735 6 27.970844291578555 26.287286256653957 25.55059902793253 20.191270423834954 7 21.703790944191756 25.787053628517253 31.495956561253614 21.013198866037378 8 26.805290434995772 26.721210564881844 25.52792004010404 20.94557896001834 9 26.388145465000857 25.93781651790939 26.48738422232681 21.186653794762947 10-11 21.049244562147816 29.467159910565726 28.63887270664838 20.84472282063808 12-13 20.988301759033124 26.322610872089687 28.618063258082504 24.07102411079468 14-15 20.98471827760108 32.17746568612607 25.986430067711012 20.851385968561836 16-17 24.018207155361594 26.155548224147495 25.779229407681793 24.047015212809118 18-19 21.06351784173608 29.125772467662475 25.991403933888662 23.819305756712787 20-21 23.776365270109693 29.44109368463402 25.963174327611515 20.819366717644773 22-23 24.28232138185616 26.29502426529952 28.616622480980958 20.80603187186336 24-25 21.115769212187523 29.396142026357232 25.603710458266676 23.88437830318857 26-27 20.91803280499827 26.720659259963313 31.515856168321505 20.845451766716913 28-29 21.15309089571659 26.566563110901274 31.483897922986316 20.79644807039582 30-31 21.152185977049196 26.26394431780833 31.714892988666644 20.868976716475828 32-33 21.186378851676917 26.360126160235716 31.611947309981858 20.841547678105506 34-35 21.190569877720915 26.70271657148139 31.303631399148983 20.803082151648706 36-37 20.918075342858877 26.651861228637063 31.564025794794926 20.866037633709137 38-39 20.981317460988635 26.381589802204413 31.55566124025008 21.08143149655687 40-41 21.252396304126645 26.431177798266187 31.275948768349753 21.04047712925741 42-43 21.2833756945836 26.4549160049875 31.459841213834082 20.801867086594818 44-45 21.568583533188683 26.53141667198451 31.162125701619992 20.737874093206816 46-47 21.107654747173882 26.63342591407181 31.3084344834478 20.950484855306502 48-49 21.452775500847046 26.609859519347673 30.94282373407266 20.99454124573262 50-51 21.513961946009672 26.62687335317308 30.889531319786514 20.969633381030732 52-53 21.371908937772773 27.001121986861016 30.929733312110976 20.697235763255232 54-55 21.717355207874984 26.946790430843777 30.248027930129435 21.08782643115181 56-57 22.189620871208067 27.66265310163105 29.21768585713963 20.930040170021254 58-59 22.399223266892818 28.154666761642567 28.563713721104712 20.882396250359907 60-61 22.561892730087667 28.609559408450956 27.96094249410956 20.86760536735182 62-63 22.73259551298849 28.724645788207287 27.73998937623719 20.802769322567034 64-65 22.894749713885318 28.802683002556655 27.637095382507482 20.665471901050545 66-67 23.074295584247608 28.629918361816603 27.704231780356643 20.591554273579153 68-69 23.26675741462642 28.437795766796803 27.802476751698972 20.492970066877806 70-71 23.47484374088393 28.31460584309059 27.839302409033177 20.3712480069923 72-73 23.67839453454314 28.17058921454655 27.825654760905007 20.325361490005307 74-75 23.936945951681007 28.146360964418367 27.725983630031536 20.19070945386909 76-77 24.27078659326906 28.205883989532595 27.459071653694224 20.06425776350412 78-79 24.585965640051317 28.26008807340386 27.182497599643046 19.971448686901777 80-81 24.864479999974737 28.36401009625908 26.882704222333693 19.88880568143249 82-83 25.050586835704692 28.484347888958382 26.624524309598417 19.840540965738505 84-85 25.231706046389373 28.50225263172681 26.451637910418686 19.81440341146513 86-87 25.428943144903194 28.46875108600319 26.347108942105237 19.75519682698838 88-89 25.680995656101413 28.470446250486237 26.188772933683474 19.65978515972888 90-91 25.88677403651317 28.412649493627583 26.05688300161077 19.643693468248475 92-93 26.006988502495997 28.372143966266115 26.038269664291658 19.582597866946234 94-95 26.119804709621164 28.39011790896084 25.978206254979213 19.51187112643879 96-97 26.232760761439955 28.356791506323592 25.88664320053574 19.523804531700712 98-99 26.4120375486064 28.32833851626177 25.812884444374284 19.44673949075755 100 26.5136803103712 28.366714358166824 25.72135268341833 19.39825264804365 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 60225.0 1 37576.0 2 12353.0 3 9328.5 4 8833.0 5 8987.0 6 9521.0 7 9775.0 8 9895.5 9 10431.0 10 11146.5 11 14830.0 12 153681.0 13 183727.0 14 75353.5 15 76428.0 16 85195.5 17 95050.5 18 104774.0 19 114892.0 20 127538.5 21 144585.5 22 167522.0 23 195771.0 24 226507.5 25 262752.5 26 304978.5 27 353516.5 28 411367.0 29 471344.0 30 528869.5 31 588072.5 32 649779.0 33 711699.0 34 774266.5 35 841580.5 36 917953.0 37 981943.5 38 1039414.0 39 1093246.0 40 1126699.0 41 1166398.0 42 1215226.5 43 1238700.5 44 1255568.5 45 1292125.5 46 1322120.5 47 1340130.0 48 1348320.0 49 1344439.5 50 1332702.5 51 1312215.0 52 1287043.5 53 1247372.0 54 1189124.5 55 1119503.0 56 1040582.0 57 944391.5 58 832705.0 59 714259.0 60 587380.5 61 470344.5 62 368717.5 63 275553.5 64 200655.5 65 145821.5 66 105596.0 67 77388.0 68 57240.5 69 43466.0 70 33653.0 71 26665.0 72 21831.0 73 17482.0 74 13927.5 75 11880.0 76 9435.5 77 6457.0 78 4294.5 79 3077.0 80 2258.5 81 1702.5 82 1357.0 83 1106.5 84 963.0 85 782.0 86 595.0 87 496.0 88 424.5 89 352.5 90 293.0 91 247.5 92 211.5 93 170.0 94 152.5 95 135.5 96 109.0 97 87.5 98 60.0 99 38.5 100 22.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1782402673859162 2 0.12693644697159367 3 0.09290926173371034 4 0.05683560539849606 5 0.04057168406842242 6 0.04102411726731821 7 0.03125471668185913 8 0.03553967994349425 9 0.024552392316821502 10-11 0.020371330865397913 12-13 0.019466464467606335 14-15 0.017742220503209883 16-17 0.0215655440764368 18-19 0.021340642689776392 20-21 0.03443884684036699 22-23 0.06403244977162909 24-25 0.009668129200822523 26-27 0.010466463362827593 28-29 0.016371768778647638 30-31 0.0029789569636597766 32-33 2.30162237810358E-4 34-35 2.7093383422247853E-4 36-37 0.0 38-39 3.814117083714504E-5 40-41 1.1968436366138615E-4 42-43 0.0 44-45 0.0 46-47 0.001249452148113372 48-49 0.008247699390335744 50-51 0.008964490359516571 52-53 0.008663306631181875 54-55 0.0028526965360609513 56-57 7.89127672492656E-6 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.31521278748776E-6 66-67 3.9456383624632796E-5 68-69 3.419553247468176E-5 70-71 0.0 72-73 0.003862779956851551 74-75 0.012783868294381027 76-77 0.01906269414184759 78-79 0.02170758705748548 80-81 0.04014029427412644 82-83 0.006766769791624525 84-85 0.004716353055931107 86-87 0.012028936154363052 88-89 0.0069785190504100545 90-91 0.00734546341811914 92-93 0.00885927333651755 94-95 0.011156950076258667 96-97 0.011280580078282518 98-99 0.019102150525472227 100 0.009466901644336896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.8016662E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.83861746961066 #Duplication Level Percentage of deduplicated Percentage of total 1 68.39398551804926 28.615097973117543 2 16.24868087114278 13.59644686707046 3 6.050308094013588 7.594095777561711 4 2.8363095903530984 4.746690879046856 5 1.6306218613751582 3.4111482147829877 6 1.016884777237901 2.5527031923316077 7 0.6928069780331028 2.029026029394276 8 0.49933796901293104 1.6713288218867461 9 0.37731074664372155 1.4207543996399867 >10 1.9740560866092822 14.641866595536603 >50 0.1519622647448082 4.536040439505371 >100 0.12589890827903613 8.421872709397734 >500 0.0012998682547780036 0.34658590418695623 >1k 4.738483162609469E-4 0.45707937464779236 >5k 3.1308968102620915E-5 0.08098997683492336 >10k+ 3.1308966209339566E-5 5.878272845058455 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1962930 5.163341273886697 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 164074 0.4315844457885335 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61179 0.16092680625142733 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.5051548186950238 1.052170229990208E-5 0.0 0.0 0.0 2 0.5052679269947478 1.841297902482864E-5 0.0 0.0 0.0 3 0.5053836657200467 3.9456383624632796E-5 0.0 2.63042557497552E-6 0.0 4 0.505452056784996 4.997808592453488E-5 0.0 5.26085114995104E-6 0.0 5 0.5055493825312701 7.102149052433904E-5 0.0 5.26085114995104E-6 1.052170229990208E-5 6 0.5058045338120427 7.102149052433904E-5 0.0 5.26085114995104E-6 1.052170229990208E-5 7 0.5061885759459892 8.680404397419216E-5 0.0 5.26085114995104E-6 1.841297902482864E-5 8 0.5064305750988869 9.20648951241432E-5 0.0 7.89127672492656E-6 1.841297902482864E-5 9 0.5065489442497608 9.995617184906975E-5 0.0 7.89127672492656E-6 2.367383017477968E-5 10-11 0.5067238675504967 1.1310829972394735E-4 0.0 7.89127672492656E-6 2.367383017477968E-5 12-13 0.507064507662456 1.249452148113372E-4 0.0 9.20648951241432E-6 2.498904296226744E-5 14-15 0.5075432451171016 1.5651032171104344E-4 0.0 1.578255344985312E-5 2.63042557497552E-5 16-17 0.5079943631032098 1.6966244958592104E-4 0.0 1.841297902482864E-5 2.761946853724296E-5 18-19 0.50825082959677 1.907058541857252E-4 0.0 1.841297902482864E-5 3.2880319687194E-5 20-21 0.5084770461962178 2.3279266338533349E-4 0.0 2.1043404599804162E-5 3.419553247468176E-5 22-23 0.5087926972652149 2.7356425979745407E-4 1.31521278748776E-6 2.498904296226744E-5 3.419553247468176E-5 24-25 0.5095029121704583 2.9592287718474596E-4 2.63042557497552E-6 2.63042557497552E-5 3.419553247468176E-5 26-27 0.5116611763547257 3.130206434220869E-4 2.63042557497552E-6 2.63042557497552E-5 3.419553247468176E-5 28-29 0.5181491210354028 3.3274883523440324E-4 2.63042557497552E-6 2.63042557497552E-5 3.419553247468176E-5 30-31 0.5334713500096353 3.524770270467197E-4 2.63042557497552E-6 3.0249894112218476E-5 3.9456383624632796E-5 32-33 0.5636252335883671 3.6562915492159727E-4 5.26085114995104E-6 3.156510689970624E-5 3.9456383624632796E-5 34-35 0.6173792954257793 3.7615085722149936E-4 5.26085114995104E-6 3.156510689970624E-5 4.077159641212056E-5 36-37 0.7046081531303301 3.89302985096377E-4 5.26085114995104E-6 3.8141170837145035E-5 4.208680919960832E-5 38-39 0.8314656873346744 4.0640075133371786E-4 5.26085114995104E-6 3.9456383624632796E-5 4.471723477458384E-5 40-41 0.9985069178351323 4.2349851757105873E-4 5.26085114995104E-6 3.9456383624632796E-5 4.471723477458384E-5 42-43 1.203680112683223 4.366506454459363E-4 5.26085114995104E-6 4.077159641212056E-5 4.734766034955936E-5 44-45 1.4530365659141773 4.458571349583506E-4 5.26085114995104E-6 4.208680919960832E-5 4.734766034955936E-5 46-47 1.7571453274882471 4.537484116832772E-4 5.26085114995104E-6 4.208680919960832E-5 5.129329871202264E-5 48-49 2.124451378713891 4.682157523456425E-4 5.26085114995104E-6 4.208680919960832E-5 5.6554149861973675E-5 50-51 2.543638628767565 4.892591569454467E-4 5.26085114995104E-6 4.866287313704712E-5 6.313021379941247E-5 52-53 3.0012511356204814 5.116177743327386E-4 5.26085114995104E-6 5.91845754369492E-5 6.839106494936352E-5 54-55 3.500707137307321 5.26085114995104E-4 5.26085114995104E-6 6.049978822443696E-5 6.839106494936352E-5 56-57 4.054715534993577 5.405524556574694E-4 5.26085114995104E-6 6.049978822443696E-5 7.496712888680232E-5 58-59 4.669709034422854 5.629110730447612E-4 5.26085114995104E-6 6.313021379941247E-5 7.891276724926559E-5 60-61 5.335065977123399 5.852696904320531E-4 5.26085114995104E-6 6.313021379941247E-5 8.022798003675335E-5 62-63 6.032024852681701 5.984218183069307E-4 5.26085114995104E-6 6.5760639374388E-5 8.417361839921664E-5 64-65 6.753736032900521 6.115739461818084E-4 5.26085114995104E-6 7.496712888680232E-5 8.417361839921664E-5 66-67 7.513130689906442 6.260412868441738E-4 5.26085114995104E-6 8.285840561172888E-5 9.074968233665543E-5 68-69 8.31797910084794 6.497151170189535E-4 5.26085114995104E-6 8.811925676167993E-5 9.732574627409423E-5 70-71 9.160159826762275 6.747041599812209E-4 5.26085114995104E-6 9.20648951241432E-5 1.0258659742404528E-4 72-73 10.024141519842011 6.944323517935373E-4 5.26085114995104E-6 9.20648951241432E-5 1.0653223578650857E-4 74-75 10.902881215610144 7.167909691808293E-4 5.26085114995104E-6 9.338010791163097E-5 1.1047787414897184E-4 76-77 11.804051865468882 7.444104377180721E-4 5.26085114995104E-6 9.469532069911872E-5 1.1047787414897184E-4 78-79 12.734802966131008 7.61508203955413E-4 5.26085114995104E-6 9.469532069911872E-5 1.1705393808641064E-4 80-81 13.68632390713314 7.759755446177783E-4 5.26085114995104E-6 1.0127138463655752E-4 1.2494521481133718E-4 82-83 14.65210306996443 7.983341620050703E-4 5.26085114995104E-6 1.0653223578650857E-4 1.2626042759882495E-4 84-85 15.628375000414291 8.141167154549234E-4 5.26085114995104E-6 1.0784744857399632E-4 1.2626042759882495E-4 86-87 16.614368983789266 8.39105758417191E-4 5.26085114995104E-6 1.0784744857399632E-4 1.2889085317380048E-4 88 17.358738649910926 8.759317164668481E-4 5.26085114995104E-6 1.0784744857399632E-4 1.31521278748776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 247360 0.0 85.25673 8 AGTGGTA 249030 0.0 84.74988 5 GTATCAA 249555 0.0 84.40346 9 TGGTATC 250250 0.0 83.98024 7 GTGGTAT 251675 0.0 83.88302 6 AAGCAGT 258285 0.0 81.893845 1 GCAGTGG 260365 0.0 81.599594 3 CAGTGGT 262425 0.0 80.36396 4 AGCAGTG 272110 0.0 78.09348 2 CAACGCA 243705 0.0 44.235004 12-13 ATCAACG 245880 0.0 43.987053 10-11 ACGCAGA 247455 0.0 43.655704 14-15 TCAACGC 243285 0.0 43.350224 12-13 GAGTACT 239355 0.0 43.218822 20-21 AGAGTAC 247950 0.0 43.08455 18-19 AACGCAG 245780 0.0 42.994118 14-15 CGCAGAG 249815 0.0 42.275383 16-17 AGTACTT 250420 0.0 42.201313 20-21 GTACTTT 248895 0.0 41.545692 22-23 TATCAAC 253920 0.0 41.446648 10-11 >>END_MODULE