FastQCFastQC Report
Thu 26 May 2016
SRR1660772_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660772_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38016662
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17478714.597644580158037No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCAATCTCGTATG1740620.457857136431389TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485020.12758090123746266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2194650.084.205658
AGTGGTA2221850.083.5639655
GTATCAA2222900.083.040389
GTGGTAT2246150.082.611816
TGGTATC2237700.082.606677
AAGCAGT2309000.080.363251
GCAGTGG2316700.080.086243
CAGTGGT2360900.078.5152364
AGCAGTG2432450.076.368012
CAACGCA2159500.043.7303712-13
ATCAACG2174250.043.51043310-11
ACGCAGA2199050.043.09346414-15
TCAACGC2148250.042.8853312-13
GAGTACT2128000.042.7369820-21
AACGCAG2179850.042.39730514-15
AGAGTAC2230500.042.27528418-19
CGCAGAG2217800.041.68986516-17
AGTACTT2246550.041.4680720-21
GTACTTT2224850.040.81639522-23
TATCAAC2264800.040.75723610-11
Produced by FastQC (version 0.11.5)