FastQCFastQC Report
Thu 26 May 2016
SRR1660771_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660771_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39328529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17731094.508454918311336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2307600.082.151595
GGTATCA2309450.082.0282758
GTGGTAT2322300.081.592246
TGGTATC2321450.081.275277
AAGCAGT2359500.080.428221
GTATCAA2355450.080.303299
GCAGTGG2391400.079.741743
CAGTGGT2425100.078.116144
AGCAGTG2518200.075.6768342
GAGTACT2174850.042.69431320-21
CAACGCA2276850.042.62257412-13
ATCAACG2293600.042.4645610-11
ACGCAGA2291100.042.3793614-15
AGAGTAC2296550.041.89817418-19
TCAACGC2270200.041.68018712-13
AACGCAG2278350.041.5860514-15
AGTACTT2297300.041.4310220-21
CGCAGAG2305250.041.02523416-17
GTACTTT2298700.040.39691522-23
TATCAAC2361950.039.96818510-11
Produced by FastQC (version 0.11.5)