FastQCFastQC Report
Thu 26 May 2016
SRR1660771_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660771_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39328529
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18781394.775512961595894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA2435000.082.499295
GGTATCA2423850.082.452288
GTGGTAT2448800.081.978726
TGGTATC2446800.081.6577767
AAGCAGT2474800.081.146811
GTATCAA2469200.080.806629
GCAGTGG2510300.080.0531853
CAGTGGT2551900.078.6395344
AGCAGTG2618800.076.7544252
GAGTACT2286800.042.95948820-21
CAACGCA2392250.042.6860912-13
ATCAACG2403000.042.5645510-11
ACGCAGA2408300.042.55087714-15
AGAGTAC2428350.041.93871718-19
TCAACGC2381950.041.808312-13
AACGCAG2391550.041.74265314-15
AGTACTT2413150.041.69460720-21
CGCAGAG2425500.041.2021516-17
GTACTTT2407400.040.78398522-23
TATCAAC2486400.040.08506810-11
Produced by FastQC (version 0.11.5)