FastQCFastQC Report
Thu 26 May 2016
SRR1660770_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660770_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33128550
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10597303.1988420863575375No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3301850.9966780918573256Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750100.22642101752114113No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1402450.083.638438
AGTGGTA1427450.081.9070745
TGGTATC1427650.081.744277
GTGGTAT1433700.081.6581346
GTATCAA1435400.081.600689
AAGCAGT1543600.076.9134751
CAGTGGT1526400.076.643664
GCAGTGG1548050.076.414513
AGCAGTG1652250.071.847032
CAACGCA1376900.043.7004312-13
TCAACGC1373700.043.27398312-13
ATCAACG1395900.043.2166510-11
ACGCAGA1394350.043.12663314-15
AACGCAG1386150.042.89016714-15
GAGTACT1356950.042.50131620-21
AGAGTAC1393700.042.3474618-19
CGCAGAG1411000.042.10872316-17
TATCAAC1436350.040.97445710-11
AGTACTT1445300.040.51501520-21
GTACTTT1452050.039.7505122-23
Produced by FastQC (version 0.11.5)