##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660770_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33128550 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.08684877545199 31.0 31.0 34.0 27.0 34.0 2 31.236409350846927 31.0 31.0 34.0 27.0 34.0 3 31.27393689129165 33.0 31.0 34.0 27.0 34.0 4 34.72600536395345 37.0 35.0 37.0 31.0 37.0 5 34.7204968524128 37.0 35.0 37.0 31.0 37.0 6 34.69568170052719 37.0 35.0 37.0 30.0 37.0 7 34.69167117184423 37.0 35.0 37.0 31.0 37.0 8 34.696678182413656 37.0 35.0 37.0 30.0 37.0 9 36.15779610034245 39.0 35.0 39.0 30.0 39.0 10-11 36.13965578028619 39.0 35.0 39.0 30.0 39.0 12-13 36.220920625865 39.0 35.0 39.0 30.0 39.0 14-15 37.37389795206853 40.0 37.0 41.0 31.0 41.0 16-17 37.33874719841345 40.0 37.0 41.0 31.0 41.0 18-19 37.29234957461162 40.0 37.0 41.0 31.0 41.0 20-21 37.25465412763312 40.0 37.0 41.0 30.5 41.0 22-23 37.16239370573116 40.0 36.5 41.0 30.5 41.0 24-25 37.14671890257799 40.0 36.0 41.0 30.0 41.0 26-27 37.02601472144117 40.0 36.0 41.0 30.0 41.0 28-29 36.97205132732945 40.0 36.0 41.0 30.0 41.0 30-31 36.80470120183347 40.0 36.0 41.0 30.0 41.0 32-33 36.68544406561712 39.5 36.0 41.0 30.0 41.0 34-35 36.57905557291218 39.0 35.5 41.0 29.5 41.0 36-37 36.38569412183751 39.0 35.0 40.5 29.0 41.0 38-39 36.073972479930454 39.0 35.0 40.0 28.0 41.0 40-41 35.941769500928956 38.0 35.0 40.0 28.0 41.0 42-43 36.16392271922556 39.0 35.0 40.0 28.0 41.0 44-45 36.24303093555257 39.0 35.0 41.0 28.0 41.0 46-47 36.12183497315759 39.0 35.0 41.0 28.0 41.0 48-49 36.0504707420035 39.0 35.0 41.0 27.5 41.0 50-51 34.9820902212744 38.0 33.5 40.0 25.5 40.5 52-53 35.00428009979308 38.0 33.5 39.5 26.0 40.5 54-55 35.416060724058255 38.5 34.0 40.0 26.0 41.0 56-57 35.21493538051016 38.5 34.0 41.0 25.0 41.0 58-59 34.94829000967444 38.0 34.0 40.0 24.0 41.0 60-61 34.714938655630874 38.0 33.5 40.0 23.5 41.0 62-63 34.434510671309184 37.0 33.0 40.0 23.0 41.0 64-65 34.11185984596368 37.0 33.0 40.0 22.5 41.0 66-67 33.72490910408092 36.0 32.5 40.0 21.5 41.0 68-69 33.41738384263724 36.0 32.0 39.0 21.0 41.0 70-71 33.127112445307745 35.0 32.0 39.0 20.5 41.0 72-73 32.79632585790806 35.0 32.0 38.5 20.0 40.0 74-75 32.4771947006434 35.0 31.0 37.0 20.0 40.0 76-77 32.10097437104854 35.0 31.0 37.0 20.0 39.0 78-79 31.749437901749396 35.0 31.0 36.5 19.0 39.0 80-81 31.46366496571688 35.0 31.0 36.0 18.5 38.5 82-83 31.180114100979367 35.0 30.0 35.5 18.0 37.0 84-85 30.91965890447967 34.5 30.0 35.0 18.0 37.0 86-87 30.704684388541 34.0 30.0 35.0 18.0 36.5 88-89 30.500958538783014 34.0 30.0 35.0 17.0 36.0 90-91 30.330504821370084 34.0 29.5 35.0 17.0 36.0 92-93 30.17569694417655 34.0 29.0 35.0 17.0 35.5 94-95 30.02014911609473 34.0 29.0 35.0 15.0 35.0 96-97 29.911528907845348 34.0 29.0 35.0 15.0 35.0 98-99 29.779179710551773 34.0 29.0 35.0 15.0 35.0 100 29.68588540699789 34.0 29.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1134996619800752 1101 2 1.0531059029551422 1101 3 1.063851985851084 1101 4 1.0501955846866835 1101 5 1.0287218085193004 1101 6 1.0341698098615737 1101 7 0.9840524810820099 1101 8 1.0507662884980746 1101 9 1.3177414759911699 1101 10-11 1.2325456908978722 1101 12-13 1.1796953950344502 1101 14-15 1.372802548100701 1101 16-17 1.3924951786018198 1101 18-19 1.3459515742452197 1101 20-21 1.2828255706379394 1101 22-23 1.250040871934857 1101 24-25 1.2174814112026127 1101 26-27 1.3653288835089157 1101 28-29 1.241320938970432 1101 30-31 1.1724335085776687 1101 32-33 1.1844003218397674 1101 34-35 1.1863286267974615 1101 36-37 1.1948511303415401 1101 38-39 1.2276258472628356 1101 40-41 1.1825644502967307 1101 42-43 1.258332886791095 1101 44-45 1.2941825960497795 1101 46-47 1.2627316445187162 1101 48-49 1.2916256957531544 1101 50-51 1.2262674553979451 1101 52-53 1.2562211527110776 1101 54-55 1.305469199432558 1101 56-57 1.2736905058334358 1101 58-59 1.27799790375267 1101 60-61 1.2700327667452527 1101 62-63 1.2374711587957208 1101 64-65 1.0983408336780514 1101 66-67 1.0475066909051165 1101 68-69 1.0129553877614441 1101 70-71 0.9987958606905352 1101 72-73 0.9658968976008921 1101 74-75 0.9036590009506611 1101 76-77 0.8764109409529155 1101 78-79 0.8954600953005674 1101 80-81 0.8981324661989518 1101 82-83 0.8560683937048204 1101 84-85 0.7643786633986274 1101 86-87 0.6690070453624948 1101 88-89 0.7119982852241726 1101 90-91 0.6800838377100753 1101 92-93 0.6993841127415656 1101 94-95 0.6345393332791822 1101 96-97 0.6554882242841202 1101 98-99 0.623839856232518 1101 100 0.46999047895359425 1102 1 1.062624324954239 1102 2 1.0688446376647782 1102 3 1.0265382438286856 1102 4 1.0066093761389325 1102 5 1.0228183513443128 1102 6 1.079663468978012 1102 7 1.00493643241213 1102 8 1.010759772137952 1102 9 1.2175382248811886 1102 10-11 1.183242959432441 1102 12-13 1.1011304153470292 1102 14-15 1.3881954897737003 1102 16-17 1.3512015093893623 1102 18-19 1.3737681564988193 1102 20-21 1.2538393831325791 1102 22-23 1.2255526311068081 1102 24-25 1.1349754063523463 1102 26-27 1.248995553853085 1102 28-29 1.2028512663783744 1102 30-31 1.1697849145343255 1102 32-33 1.1724836958437592 1102 34-35 1.1598566995311188 1102 36-37 1.1696046611069093 1102 38-39 1.126114215378898 1102 40-41 1.092720612129476 1102 42-43 1.1954935452613071 1102 44-45 1.2098504979491338 1102 46-47 1.1619486862791817 1102 48-49 1.1472269195380846 1102 50-51 1.0786835064057385 1102 52-53 1.1500624016726704 1102 54-55 1.2567571612252948 1102 56-57 1.1717696108407623 1102 58-59 1.1825339512472866 1102 60-61 1.1711296236820985 1102 62-63 1.167285905036202 1102 64-65 1.0979577167382075 1102 66-67 1.0354552330404374 1102 68-69 0.9813370671837944 1102 70-71 0.9583967017721093 1102 72-73 0.98907605638923 1102 74-75 0.8645763155233794 1102 76-77 0.8485613533783436 1102 78-79 0.8844712267734174 1102 80-81 0.8939985918506892 1102 82-83 0.8546507253573346 1102 84-85 0.8061707945144292 1102 86-87 0.7195184939344621 1102 88-89 0.7121871769516197 1102 90-91 0.7182634016312228 1102 92-93 0.697794020263828 1102 94-95 0.6327814284598112 1102 96-97 0.6685819353317761 1102 98-99 0.6633594644566578 1102 100 0.622851323499976 1103 1 0.8880945423272131 1103 2 0.9253842524742417 1103 3 0.9288840953541033 1103 4 0.9170366958169041 1103 5 0.8814123018149544 1103 6 0.9417736506367973 1103 7 0.8718374283629728 1103 8 0.9141122853221475 1103 9 1.121064655150235 1103 10-11 1.0792527383858683 1103 12-13 0.9874340301777238 1103 14-15 1.2066244340899672 1103 16-17 1.2175906029205095 1103 18-19 1.1990347739590632 1103 20-21 1.108213134083357 1103 22-23 1.0979699131106884 1103 24-25 1.0447964176208941 1103 26-27 1.1359468784825708 1103 28-29 1.0631634253669304 1103 30-31 1.0457176789967733 1103 32-33 1.0522957351509277 1103 34-35 1.068704436816887 1103 36-37 1.0970010136026715 1103 38-39 1.0899059765307229 1103 40-41 1.0645442113597525 1103 42-43 1.0668784634191795 1103 44-45 1.0424349632955057 1103 46-47 1.0476513439147013 1103 48-49 1.0265394261930254 1103 50-51 1.0174309116226041 1103 52-53 0.9916995034835523 1103 54-55 1.0699808516076246 1103 56-57 1.118477046356638 1103 58-59 1.1573476743570765 1103 60-61 1.1253191913541158 1103 62-63 1.0715240289740962 1103 64-65 0.9906865585372486 1103 66-67 0.9189049539729908 1103 68-69 0.9322292728397983 1103 70-71 0.8899396696106905 1103 72-73 0.9168185264296156 1103 74-75 0.8752772371339788 1103 76-77 0.8429207299798165 1103 78-79 0.9075594868678927 1103 80-81 0.8257113346427616 1103 82-83 0.7904707080769917 1103 84-85 0.7241801985997292 1103 86-87 0.667482077448124 1103 88-89 0.6719879966406204 1103 90-91 0.6601281140843618 1103 92-93 0.6859971410315779 1103 94-95 0.7088399155223151 1103 96-97 0.6730980329859939 1103 98-99 0.6296082487935344 1103 100 0.5847552416982715 1104 1 0.9391641216800188 1104 2 0.8982270462274293 1104 3 0.9384109590035798 1104 4 0.9278630809995931 1104 5 0.9159297253687413 1104 6 0.951400025056266 1104 7 0.9206706241548375 1104 8 0.9001788711183067 1104 9 1.1351305851196685 1104 10-11 1.0608647829947628 1104 12-13 1.0196205216591565 1104 14-15 1.2101716492382764 1104 16-17 1.2483598878081992 1104 18-19 1.2192225241632073 1104 20-21 1.1237416923552104 1104 22-23 1.1061302935090822 1104 24-25 1.0064804168385777 1104 26-27 1.1327111243715038 1104 28-29 1.0913296492785491 1104 30-31 1.0738248412596576 1104 32-33 1.0960965691963693 1104 34-35 1.0937800530501676 1104 36-37 1.0917685148040732 1104 38-39 1.1116487108884954 1104 40-41 1.0374688904952123 1104 42-43 1.081222706302377 1104 44-45 1.0854459306041093 1104 46-47 1.064447134363597 1104 48-49 1.1251597548138221 1104 50-51 1.1040222738977192 1104 52-53 1.1305632313752554 1104 54-55 1.213644125293463 1104 56-57 1.1065881372881918 1104 58-59 1.1352264980620959 1104 60-61 1.1392822979545159 1104 62-63 1.1523725456235283 1104 64-65 1.0408846823555251 1104 66-67 0.9519888691093783 1104 68-69 0.9911423710582241 1104 70-71 0.9832141834617758 1104 72-73 0.9450165689984615 1104 74-75 0.8925720186138975 1104 76-77 0.9076569169243598 1104 78-79 0.9162090459215655 1104 80-81 0.8846945817567473 1104 82-83 0.9108465522282962 1104 84-85 0.8626223145275667 1104 86-87 0.8169647819834687 1104 88-89 0.8533793888067365 1104 90-91 0.7275251812446335 1104 92-93 0.7738258512662917 1104 94-95 0.8245010960941279 1104 96-97 0.8077603317672946 1104 98-99 0.8492928947367808 1104 100 0.8270578254186844 1105 1 0.6949392630842226 1105 2 0.7157303865090405 1105 3 0.7275243278294603 1105 4 0.7120631662896955 1105 5 0.68059032536042 1105 6 0.7806280717322522 1105 7 0.8117946676832943 1105 8 0.7951196343903675 1105 9 0.9768125343399561 1105 10-11 0.9080275875698547 1105 12-13 0.8238479617153445 1105 14-15 0.9927212022848764 1105 16-17 0.9828530364343422 1105 18-19 0.9472011736082777 1105 20-21 0.9001648544236076 1105 22-23 0.8883980258697477 1105 24-25 0.7824384570466236 1105 26-27 0.9572018550863888 1105 28-29 0.9246474124031323 1105 30-31 0.8251255394446915 1105 32-33 0.8267815225972228 1105 34-35 0.8827116452807218 1105 36-37 0.8696109472436362 1105 38-39 0.8959004618024196 1105 40-41 0.8322737002241993 1105 42-43 0.8899824773697347 1105 44-45 0.8945583693027288 1105 46-47 0.9471227776336306 1105 48-49 0.9269674748122512 1105 50-51 0.8586844619379335 1105 52-53 0.8870147401150419 1105 54-55 0.9798118514326077 1105 56-57 0.9853396272060948 1105 58-59 0.9278993565421629 1105 60-61 0.9886076622801454 1105 62-63 0.9542615854412801 1105 64-65 0.881427234921297 1105 66-67 0.7197059386962295 1105 68-69 0.7531156233565284 1105 70-71 0.7609221493196756 1105 72-73 0.8119115210267083 1105 74-75 0.7093979591532431 1105 76-77 0.7473092386918978 1105 78-79 0.7178944502197915 1105 80-81 0.7536354389731912 1105 82-83 0.688124991150115 1105 84-85 0.6835391347060416 1105 86-87 0.6890783941125811 1105 88-89 0.6738250890915118 1105 90-91 0.6888276752165474 1105 92-93 0.6871472579823923 1105 94-95 0.6831439336090455 1105 96-97 0.6352200751224295 1105 98-99 0.7016191469127548 1105 100 0.693533755272064 1106 1 0.6756150371831211 1106 2 0.677633392418489 1106 3 0.6500639643886181 1106 4 0.7063684752042363 1106 5 0.6992193797744193 1106 6 0.76856251246776 1106 7 0.7454769411246076 1106 8 0.7455710332856924 1106 9 0.9008659282769926 1106 10-11 0.8486995637992365 1106 12-13 0.7912499298064688 1106 14-15 0.8348558867957152 1106 16-17 0.8909969306343513 1106 18-19 0.8634793913847503 1106 20-21 0.830528632102471 1106 22-23 0.8118595334337471 1106 24-25 0.7187401737579577 1106 26-27 0.817812420883385 1106 28-29 0.7799654444389503 1106 30-31 0.7425487956026231 1106 32-33 0.7127246882242062 1106 34-35 0.709867049875271 1106 36-37 0.7816050758263131 1106 38-39 0.7990550335041675 1106 40-41 0.7700534266090386 1106 42-43 0.6405066851779182 1106 44-45 0.699509089065188 1106 46-47 0.6931874171268362 1106 48-49 0.6811153475481859 1106 50-51 0.6706236182245462 1106 52-53 0.653548355055598 1106 54-55 0.7687807682677388 1106 56-57 0.8263968564194215 1106 58-59 0.8412970597085732 1106 60-61 0.8368487593863563 1106 62-63 0.798560056574253 1106 64-65 0.776272200977111 1106 66-67 0.7321647118340238 1106 68-69 0.7744162675366866 1106 70-71 0.7429211822455315 1106 72-73 0.6908857412847809 1106 74-75 0.7058815034091808 1106 76-77 0.7327272615807843 1106 78-79 0.7473615913453493 1106 80-81 0.7394499734154252 1106 82-83 0.6881136626338709 1106 84-85 0.6638399885518318 1106 86-87 0.554198915175018 1106 88-89 0.646808681472006 1106 90-91 0.6618395038465685 1106 92-93 0.6395583636052216 1106 94-95 0.632286760772697 1106 96-97 0.6279510273837943 1106 98-99 0.598077268800278 1106 100 0.6704295389228996 1107 1 0.8086463785658395 1107 2 0.8537629555967321 1107 3 0.8419882227029198 1107 4 0.8207079590493578 1107 5 0.7823609176236133 1107 6 0.8664192647038291 1107 7 0.8715144367938166 1107 8 0.886394609292374 1107 9 1.0873332273009524 1107 10-11 1.043815268177049 1107 12-13 0.9301242207908089 1107 14-15 1.1752559765736734 1107 16-17 1.166210711228068 1107 18-19 1.1429042156072313 1107 20-21 1.087730039237826 1107 22-23 1.068420451061229 1107 24-25 1.0022955723544413 1107 26-27 1.1181552224731917 1107 28-29 1.0880742133684436 1107 30-31 1.0527880816321797 1107 32-33 1.0459440062204592 1107 34-35 1.0779281145726998 1107 36-37 1.104575101464924 1107 38-39 1.0981215009835594 1107 40-41 1.1645229708522038 1107 42-43 1.1090986602296624 1107 44-45 1.079380213261743 1107 46-47 1.110880758462308 1107 48-49 1.0913873183972314 1107 50-51 1.0724091489545913 1107 52-53 1.0504433662914536 1107 54-55 1.1083895539962825 1107 56-57 1.1114844860383144 1107 58-59 1.1110191206558184 1107 60-61 1.0729828535536399 1107 62-63 1.0661276622065046 1107 64-65 1.016119723797658 1107 66-67 0.90926050978387 1107 68-69 0.9182514296904998 1107 70-71 0.9119047463189034 1107 72-73 0.9239547998100264 1107 74-75 0.8568876829978294 1107 76-77 0.8658812537450089 1107 78-79 0.9023756666908334 1107 80-81 0.9190434568480441 1107 82-83 0.852834071520757 1107 84-85 0.8259822983725194 1107 86-87 0.8130834322157767 1107 88-89 0.8224958141072882 1107 90-91 0.8787846174162688 1107 92-93 0.853214695725736 1107 94-95 0.8673683460368125 1107 96-97 0.8934704154797437 1107 98-99 0.9146112316299515 1107 100 0.9168871362413427 1108 1 0.4588581346084766 1108 2 0.4700662593996583 1108 3 0.4886472987734294 1108 4 0.547025942053132 1108 5 0.5058780683943951 1108 6 0.5289460076346302 1108 7 0.4996515792908198 1108 8 0.5336165780880222 1108 9 0.6835914471657389 1108 10-11 0.5934213016446179 1108 12-13 0.5357478262595308 1108 14-15 0.6210929576543762 1108 16-17 0.6629716523332476 1108 18-19 0.6103511315526617 1108 20-21 0.5599495010562805 1108 22-23 0.5286511590871896 1108 24-25 0.4813258229812689 1108 26-27 0.5252149950854346 1108 28-29 0.5081413340235912 1108 30-31 0.5045115209108175 1108 32-33 0.5025044810422585 1108 34-35 0.48777982114769713 1108 36-37 0.42155457984495825 1108 38-39 0.4163677610262013 1108 40-41 0.5306780446539605 1108 42-43 0.5250812145222383 1108 44-45 0.5118452941949485 1108 46-47 0.529801036531218 1108 48-49 0.535659094443659 1108 50-51 0.3711742687127213 1108 52-53 0.3908026316384863 1108 54-55 0.49848347103146295 1108 56-57 0.5100404459591275 1108 58-59 0.5252170721576093 1108 60-61 0.47690389018431034 1108 62-63 0.4573412493224325 1108 64-65 0.4256701574859534 1108 66-67 0.4225529907658583 1108 68-69 0.3893143170970106 1108 70-71 0.45995852860720277 1108 72-73 0.45969651659853383 1108 74-75 0.39729036843268517 1108 76-77 0.3914386232668292 1108 78-79 0.44386597458479216 1108 80-81 0.48442469299897084 1108 82-83 0.45549131869872284 1108 84-85 0.4390023885070171 1108 86-87 0.3674976800167009 1108 88-89 0.3331900071907903 1108 90-91 0.37797439099400165 1108 92-93 0.37004485531112863 1108 94-95 0.3604358335895945 1108 96-97 0.3780374368339885 1108 98-99 0.38754301873842323 1108 100 0.36922690519779167 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76-77 -2.516202608583324 2316 78-79 -5.652519961451599 2316 80-81 -3.1580149099760675 2316 82-83 -2.3627810022700864 2316 84-85 -2.195472811495801 2316 86-87 -2.2831024110274107 2316 88-89 -2.0839115778602206 2316 90-91 -2.1527913322503487 2316 92-93 -2.148838442824303 2316 94-95 -2.1225391072595308 2316 96-97 -2.10233620529905 2316 98-99 -2.1665602865412303 2316 100 -2.2366557833921306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 25.0 4 103.0 5 300.0 6 1395.0 7 14543.0 8 28203.0 9 42030.0 10 47999.0 11 50261.0 12 55378.0 13 66109.0 14 80821.0 15 99710.0 16 122532.0 17 147179.0 18 170635.0 19 195851.0 20 226870.0 21 272400.0 22 337024.0 23 429527.0 24 540419.0 25 621238.0 26 661049.0 27 646402.0 28 593036.0 29 648718.0 30 750569.0 31 891596.0 32 1086856.0 33 1370461.0 34 1798365.0 35 2500844.0 36 3759262.0 37 6072375.0 38 6885454.0 39 1908792.0 40 4219.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.49938631536717 32.936662298432694 27.936580465762567 20.627370920437567 2 22.95130176120367 31.184296303756415 26.092194371753553 19.772207563286358 3 24.166898322985347 27.408860205934193 26.19111219151989 22.233129279560572 4 20.519987409011758 27.145777635999696 27.25908807823703 25.075146876751514 5 21.354083861048025 30.13303725829865 26.179506437033034 22.333372443620288 6 26.58227118301284 26.548732135876758 25.866245277864564 21.00275140324584 7 22.46520297447368 26.461013234808046 29.76992352517692 21.303860265541353 8 25.058407325403614 27.984759369184587 25.897203469514967 21.059629835896832 9 24.748220375371943 27.497939461445174 26.422004031275208 21.33183613190767 10-11 21.501813390564937 29.18739576588773 28.171146941233467 21.139643902313864 12-13 21.557580998866538 26.620718383388347 28.18497489325672 23.636725724488393 14-15 21.5801083959304 30.45388494214205 26.839200629064656 21.126806032862895 16-17 23.574845563720718 26.523702365482343 26.220438262465457 23.681013808331485 18-19 21.640284588368644 28.467697801443165 26.778702961644868 23.113314648543327 20-21 23.026308139591954 29.07651066450818 26.755748756570735 21.14143243932913 22-23 24.24856924093896 26.583513473680338 28.032330622133134 21.135586663247572 24-25 21.756570335737884 29.05787656442012 26.04074238284937 23.144810716992627 26-27 21.24138274440267 27.75682500956395 29.825686536703238 21.17610570933014 28-29 21.785494268139367 27.25299819322228 29.82982686151509 21.131680677123267 30-31 21.835400854788617 26.616950069058294 30.380453147002985 21.167195929150104 32-33 21.81885919708321 26.64574455454179 30.377452263426623 21.157943984948375 34-35 21.839685917475553 27.28086743265993 29.740754971133327 21.138691678731185 36-37 21.254185666067755 27.249240027731137 30.28162967236848 21.214944633832626 38-39 21.27793793239198 26.62458427060968 30.251921307973056 21.845556489025288 40-41 21.913869605711312 26.705574434622818 29.619008084515496 21.761547875150374 42-43 21.88485599926561 26.744892775232625 30.21183439784501 21.158416827656755 44-45 22.545952342480792 26.757431384976478 29.564516626774 21.132099645768726 46-47 21.3694294061758 26.84784013684539 30.067924384706124 21.71480607227268 48-49 22.01091210498076 26.834042758903422 29.37898272001187 21.776062416103947 50-51 22.05716248849309 26.887285933676274 29.32755752578989 21.72799405204075 52-53 21.495954737933793 27.627188974078305 29.79305109736778 21.08380519062013 54-55 21.75994290192316 27.178346705353647 29.286646331606175 21.775064061117018 56-57 22.081181604211757 28.0642760946187 28.596893309311206 21.257648991858336 58-59 22.219209985899592 28.441622759413537 28.142917655994047 21.19624959869282 60-61 22.32181790143654 29.237814189464757 27.241310167706406 21.199057741392302 62-63 22.417806248117564 29.355947408797515 27.094676833270658 21.131569509814263 64-65 22.502177598698452 29.358364270574967 27.095752977235243 21.043705153491338 66-67 22.699443899867322 29.057389495623188 27.202471035126425 21.040695569383068 68-69 22.85709034752314 28.799139536036694 27.324720327541456 21.01904978889871 70-71 23.00356454002344 28.624174415444024 27.437925904255668 20.93433514027687 72-73 23.193751105715503 28.45710261802472 27.44990877540371 20.899237500856067 74-75 23.417629016617543 28.43645406886422 27.359814829442325 20.786102085075914 76-77 23.705613472105938 28.448832771892903 27.139571882969676 20.705981873031487 78-79 23.96618417695424 28.44138530174252 26.88887796371478 20.70355255758846 80-81 24.21587271198476 28.476755708346165 26.667633980371587 20.639737599297494 82-83 24.374928867532464 28.56090755561861 26.50921996056834 20.554943616280585 84-85 24.56012471828915 28.559310082841154 26.37437983294356 20.50618536592614 86-87 24.764392705939056 28.518735502775343 26.257120512156355 20.45975127912924 88-89 24.95922514000593 28.494169420417297 26.167288204619325 20.379317234957444 90-91 25.184669379113316 28.44235670334504 25.995935920637532 20.377037996904114 92-93 25.31655010092197 28.431646750300455 25.94140108114181 20.310402067635767 94-95 25.469331623613776 28.434568713633208 25.90124098379225 20.194858678960763 96-97 25.638116252717058 28.408515205182084 25.79468053514355 20.158688006957313 98-99 25.784414362990155 28.394977639023715 25.714598784951047 20.106009213035083 100 25.861539618461265 28.420872857366774 25.642197277035827 20.075390247136134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56229.0 1 34377.5 2 10394.0 3 8016.5 4 7771.0 5 7938.5 6 8311.5 7 8500.5 8 8676.0 9 9015.5 10 9818.0 11 12170.0 12 67121.0 13 84503.5 14 48748.0 15 52365.5 16 59645.5 17 67259.0 18 74839.0 19 83289.0 20 94474.0 21 111647.5 22 137125.0 23 165679.5 24 194791.5 25 230141.0 26 269896.5 27 316793.5 28 374766.0 29 430659.0 30 478838.5 31 529880.5 32 586111.0 33 636137.5 34 681355.5 35 738365.0 36 798456.0 37 849441.0 38 914082.5 39 969728.5 40 1003359.0 41 1051645.0 42 1107762.5 43 1124366.5 44 1114449.5 45 1123475.0 46 1137704.0 47 1144718.5 48 1142740.0 49 1134470.0 50 1124845.0 51 1117377.5 52 1102365.0 53 1057571.5 54 998396.0 55 941903.0 56 882263.0 57 812015.0 58 728991.5 59 632124.0 60 524258.5 61 426489.0 62 341524.0 63 264170.0 64 201377.5 65 152264.0 66 114208.5 67 87244.0 68 67922.5 69 53120.5 70 41873.5 71 34006.5 72 28502.5 73 23486.5 74 19464.0 75 16631.5 76 13221.5 77 9134.5 78 6208.0 79 4626.5 80 3480.5 81 2691.0 82 2082.5 83 1595.5 84 1256.0 85 1015.5 86 833.0 87 670.0 88 574.5 89 519.0 90 437.5 91 368.0 92 333.0 93 286.5 94 223.5 95 177.0 96 144.5 97 121.5 98 100.0 99 72.5 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03680209366241505 2 0.012895221795098185 3 0.003534715524826773 4 2.837431762030031E-4 5 3.320398870460675E-5 6 0.0 7 0.0 8 0.0 9 2.2639083207686423E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.05524841866003795 22-23 0.07325705471564556 24-25 0.04890343827303037 26-27 0.03623158876558135 28-29 0.038302310242977734 30-31 0.03392693009503887 32-33 0.03499700409465552 34-35 0.036699463151873536 36-37 0.03834607913717926 38-39 0.05227364312654795 40-41 0.07328422161549479 42-43 0.08737025918731728 44-45 0.08281527564593077 46-47 0.05050024827527918 48-49 0.031871301339780944 50-51 0.019926921039405587 52-53 0.0297854871402461 54-55 0.02836224344258955 56-57 0.029107823916229354 58-59 0.03554034209163999 60-61 0.06362638871909576 62-63 0.09506452893350298 64-65 0.12051085845894251 66-67 0.09358544216393412 68-69 0.05726329706552204 70-71 0.05889029251204777 72-73 0.05835601014834637 74-75 0.04877515013485347 76-77 0.05398364854483519 78-79 0.04348816956975177 80-81 0.038592090508036116 82-83 0.03778915769027018 84-85 0.05073116692399758 86-87 0.05208649337203107 88-89 0.06103647760013644 90-91 0.052776230773758585 92-93 0.04930792322634102 94-95 0.055077870899873364 96-97 0.051046604816691335 98-99 0.042754663273822734 100 0.05083228816232525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.312855E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.78077887377962 #Duplication Level Percentage of deduplicated Percentage of total 1 61.66605418844689 23.297915573122236 2 18.542131818431418 14.010723641612612 3 8.1798303364032 9.27121083494051 4 3.852974483306032 5.822735078404019 5 2.056070083388413 3.883996458474563 6 1.2699966386537458 2.8788877305252343 7 0.8318345369945299 2.1999149691234234 8 0.5651211863697024 1.7080574863297382 9 0.46551935947584216 1.5828915583638286 >10 2.2350784152665324 15.056984520744088 >50 0.18460146663482677 4.915155584741271 >100 0.1474659738688396 9.846351404467049 >500 0.0027902070445641283 0.6203144672905173 >1k 4.6744296428761144E-4 0.3320399920838697 >5k 1.5965688112031728E-5 0.032424808037545265 >10k+ 4.789706296575374E-5 4.540395891739535 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1059730 3.1988420863575375 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 330185 0.9966780918573256 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75010 0.22642101752114113 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2069438596014617 0.0 0.0 3.0185444276915227E-6 4.225962198768132E-5 2 1.206989137767877 0.0 0.0 3.0185444276915227E-6 4.225962198768132E-5 3 1.2069951748567322 6.037088855383045E-6 0.0 6.037088855383045E-6 4.5278166415372845E-5 4 1.2069951748567322 1.5092722138457614E-5 0.0 6.037088855383045E-6 5.131525527075589E-5 5 1.2069951748567322 3.0185444276915228E-5 0.0 9.05563328307457E-6 6.0370888553830455E-5 6 1.2073483445547721 3.0185444276915228E-5 0.0 9.05563328307457E-6 6.0370888553830455E-5 7 1.2073483445547721 4.829671084306436E-5 0.0 1.5092722138457614E-5 6.64079774092135E-5 8 1.2073513630991999 5.131525527075589E-5 0.0 1.811126656614914E-5 6.942652183690502E-5 9 1.2073543816436276 5.433379969844741E-5 0.0 3.0185444276915228E-5 7.244506626459655E-5 10-11 1.2073921134489738 6.338943298152199E-5 6.037088855383045E-6 3.320398870460675E-5 7.848215511997959E-5 12-13 1.2074539936097415 6.64079774092135E-5 6.037088855383045E-6 3.622253313229828E-5 7.999142733382535E-5 14-15 1.2075204015871508 6.791724962305926E-5 6.037088855383045E-6 3.622253313229828E-5 8.150069954767112E-5 16-17 1.207576244659063 6.942652183690502E-5 6.037088855383045E-6 3.622253313229828E-5 9.206560504459145E-5 18-19 1.2076381248198307 8.753778840305416E-5 6.037088855383045E-6 5.131525527075589E-5 1.0112123832766602E-4 20-21 1.2077467924192276 9.961196611382026E-5 6.037088855383045E-6 6.489870519536775E-5 1.0413978275535755E-4 22-23 1.2079535627125244 1.1772323267996939E-4 6.037088855383045E-6 6.942652183690502E-5 1.056490549692033E-4 24-25 1.2086146239421889 1.2074177710766091E-4 9.05563328307457E-6 7.395433847844231E-5 1.1621396046612363E-4 26-27 1.2105570572814084 1.2376032153535243E-4 9.05563328307457E-6 7.697288290613383E-5 1.3432522703227278E-4 28-29 1.215733860974899 1.3734377145996427E-4 9.05563328307457E-6 8.150069954767112E-5 1.4790867695688463E-4 30-31 1.2272647006886808 1.6149212688149647E-4 9.05563328307457E-6 8.150069954767112E-5 1.494179491707304E-4 32-33 1.2500667852954628 1.7960339344764563E-4 9.05563328307457E-6 8.150069954767112E-5 1.6300139909534224E-4 34-35 1.2897757372417447 1.9922393222764051E-4 9.05563328307457E-6 8.451924397536264E-5 1.7507557680610832E-4 36-37 1.348622260859591 2.33937193146093E-4 9.05563328307457E-6 8.451924397536264E-5 1.8262193787533712E-4 38-39 1.4321710428014507 2.611040929953167E-4 9.05563328307457E-6 9.961196611382026E-5 1.841312100891829E-4 40-41 1.543347052617757 2.7166899849223705E-4 9.05563328307457E-6 1.0715832718304907E-4 1.9318684337225748E-4 42-43 1.683104150347661 2.777060873476201E-4 9.05563328307457E-6 1.1621396046612363E-4 2.007332044414863E-4 44-45 1.8565240555351803 2.9128953727223194E-4 9.05563328307457E-6 1.1923250489381515E-4 2.0224247665533203E-4 46-47 2.0668788703399334 3.1091007605222687E-4 9.05563328307457E-6 1.2828813817688972E-4 2.0677029329686932E-4 48-49 2.3171554444731206 3.184564371214556E-4 9.05563328307457E-6 1.4790867695688463E-4 2.0827956551071507E-4 50-51 2.6075650760446805 3.4562333697067937E-4 9.05563328307457E-6 1.5696431023995918E-4 2.1280738215225236E-4 52-53 2.932283483581382 3.561882424675997E-4 9.05563328307457E-6 1.5847358245380495E-4 2.2488155986301843E-4 54-55 3.293429685271465 3.682624201783658E-4 9.05563328307457E-6 1.6300139909534224E-4 2.2790010429070998E-4 56-57 3.698622487250423 3.8939223117220645E-4 9.05563328307457E-6 1.675292157368795E-4 2.3091864871840152E-4 58-59 4.14566589844711 4.2108694766296745E-4 9.05563328307457E-6 1.7356630459226257E-4 2.4752064307070486E-4 60-61 4.629863063732038 4.346703975875793E-4 9.05563328307457E-6 1.841312100891829E-4 2.6412263742300825E-4 62-63 5.149011954945205 4.588187530091115E-4 1.207417771076609E-5 1.841312100891829E-4 2.7166899849223705E-4 64-65 5.705797567355045 4.8296710843064364E-4 1.207417771076609E-5 1.8714975451687441E-4 2.852524484168489E-4 66-67 6.297071559123475 4.905134694998725E-4 1.207417771076609E-5 2.007332044414863E-4 3.0487298719684383E-4 68-69 6.92970413736792 5.010783749967928E-4 1.3583449924611851E-5 2.2186301543532694E-4 3.078915316245353E-4 70-71 7.597259765368541 5.176803693490962E-4 1.5092722138457614E-5 2.4902991528455063E-4 3.169471649076099E-4 72-73 8.290236065266967 5.493750858398571E-4 1.5092722138457614E-5 2.5355773192608795E-4 3.2298425376299294E-4 74-75 9.013778146040199 5.523936302675486E-4 1.5092722138457614E-5 2.65631909636854E-4 3.260027981906845E-4 76-77 9.76817126013665 5.584307191229317E-4 2.112981099384066E-5 2.65631909636854E-4 3.2751207040453026E-4 78-79 10.555113338796899 5.765419856890809E-4 2.112981099384066E-5 2.6865045406454556E-4 3.3807697590145056E-4 80-81 11.370876479652747 6.006903411106131E-4 2.112981099384066E-5 2.7166899849223705E-4 3.441140647568336E-4 82-83 12.207380039271262 6.112552466075334E-4 2.112981099384066E-5 2.8072463177531164E-4 3.441140647568336E-4 84-85 13.065269986159974 6.142737910352249E-4 2.112981099384066E-5 2.988358983414608E-4 3.441140647568336E-4 86-87 13.946979267127599 6.414406908844487E-4 2.112981099384066E-5 3.0336371498299806E-4 3.441140647568336E-4 88 14.619501909983986 6.550241408090604E-4 2.112981099384066E-5 3.0487298719684383E-4 3.4713260918452514E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 140245 0.0 83.63843 8 AGTGGTA 142745 0.0 81.907074 5 TGGTATC 142765 0.0 81.74427 7 GTGGTAT 143370 0.0 81.658134 6 GTATCAA 143540 0.0 81.60068 9 AAGCAGT 154360 0.0 76.913475 1 CAGTGGT 152640 0.0 76.64366 4 GCAGTGG 154805 0.0 76.41451 3 AGCAGTG 165225 0.0 71.84703 2 CAACGCA 137690 0.0 43.70043 12-13 TCAACGC 137370 0.0 43.273983 12-13 ATCAACG 139590 0.0 43.21665 10-11 ACGCAGA 139435 0.0 43.126633 14-15 AACGCAG 138615 0.0 42.890167 14-15 GAGTACT 135695 0.0 42.501316 20-21 AGAGTAC 139370 0.0 42.34746 18-19 CGCAGAG 141100 0.0 42.108723 16-17 TATCAAC 143635 0.0 40.974457 10-11 AGTACTT 144530 0.0 40.515015 20-21 GTACTTT 145205 0.0 39.75051 22-23 >>END_MODULE