FastQCFastQC Report
Thu 26 May 2016
SRR1660770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33128550
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12914593.898326368042066No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTTGCATCTCGTATG2044640.6171836678635195TruSeq Adapter, Index 14 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACTGTTGCATCTCGTATG1333010.4023749907557077TruSeq Adapter, Index 4 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA496470.14986167520160104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1636850.085.157958
AGTGGTA1675550.083.61465
TGGTATC1674600.083.20747
GTGGTAT1681050.083.187286
GTATCAA1677850.083.012619
GCAGTGG1784300.078.7028053
AAGCAGT1737600.078.6956251
CAGTGGT1779600.078.678274
AGCAGTG1877700.074.667862
CAACGCA1597850.044.15798612-13
ATCAACG1618550.043.66724810-11
TCAACGC1593250.043.61146512-13
ACGCAGA1626200.043.53059814-15
AACGCAG1612950.043.23103314-15
AGAGTAC1639700.042.9371318-19
GAGTACT1604450.042.8862320-21
CGCAGAG1637650.042.5861716-17
TATCAAC1669750.041.6640310-11
AGTACTT1695700.041.19512620-21
GTACTTT1700600.040.3693322-23
Produced by FastQC (version 0.11.5)