FastQCFastQC Report
Thu 26 May 2016
SRR1660769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31955243
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16901205.289022524410157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1267560.39666730119999394No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2112000.088.0279548
GTGGTAT2131600.087.081946
TGGTATC2136000.086.660687
GTATCAA2153000.086.355999
AGTGGTA2150850.086.154075
GCAGTGG2250550.083.212233
AAGCAGT2264250.082.835791
CAGTGGT2268000.081.720484
AGCAGTG2344750.080.1177142
CAACGCA2094250.045.5744512-13
ATCAACG2110750.045.2905110-11
TCAACGC2094100.045.0842912-13
ACGCAGA2127350.044.84925514-15
AACGCAG2115500.044.67396514-15
AGAGTAC2119800.044.29653518-19
CGCAGAG2138800.044.05684716-17
GAGTACT2087000.043.9942720-21
TATCAAC2150200.043.41872410-11
AGTACTT2200800.042.30067420-21
GTACTTT2203400.041.73161322-23
Produced by FastQC (version 0.11.5)