##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660769_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31955243 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13586577952169 31.0 31.0 34.0 27.0 34.0 2 31.285822079337652 31.0 31.0 34.0 27.0 34.0 3 31.3311394001917 33.0 31.0 34.0 27.0 34.0 4 34.7893322231973 37.0 35.0 37.0 31.0 37.0 5 34.77490767321031 37.0 35.0 37.0 31.0 37.0 6 34.762022807963 37.0 35.0 37.0 31.0 37.0 7 34.72718708476102 37.0 35.0 37.0 31.0 37.0 8 34.763982955786005 37.0 35.0 37.0 31.0 37.0 9 36.231150863099366 39.0 37.0 39.0 30.0 39.0 10-11 36.17513587676363 39.0 35.0 39.0 30.5 39.0 12-13 36.3079931202526 39.0 37.0 39.0 31.0 39.0 14-15 37.48896489380475 40.0 37.0 41.0 31.0 41.0 16-17 37.44378528118219 40.0 37.0 41.0 31.0 41.0 18-19 37.41403305867522 40.0 37.0 41.0 31.0 41.0 20-21 37.37165750859726 40.0 37.0 41.0 31.0 41.0 22-23 37.18761431418312 40.0 36.5 41.0 30.5 41.0 24-25 37.22479717960524 40.0 36.5 41.0 31.0 41.0 26-27 37.14633908432491 40.0 36.0 41.0 30.0 41.0 28-29 37.13964240547318 40.0 36.0 41.0 30.0 41.0 30-31 37.043488528627364 40.0 36.0 41.0 30.0 41.0 32-33 36.93012639584684 40.0 36.0 41.0 30.0 41.0 34-35 36.78900697140685 40.0 36.0 41.0 30.0 41.0 36-37 36.569189272007726 39.0 35.5 40.5 29.5 41.0 38-39 36.14975614486799 38.0 35.0 40.0 29.0 41.0 40-41 36.00805676864982 38.0 35.0 40.0 28.5 41.0 42-43 36.223964859225134 39.0 35.0 40.0 29.0 41.0 44-45 36.28840977676183 39.0 35.0 41.0 29.0 41.0 46-47 36.22059744624693 39.0 35.0 41.0 28.0 41.0 48-49 36.10293342159845 39.0 35.0 41.0 28.0 41.0 50-51 35.02542900393529 38.0 34.0 40.0 26.0 40.5 52-53 35.06777260307487 38.0 33.5 39.5 26.0 40.5 54-55 35.28375140192175 38.5 34.0 40.5 25.0 41.0 56-57 34.9635474529172 39.0 34.0 41.0 23.0 41.0 58-59 34.68311760295486 38.0 33.5 40.5 22.0 41.0 60-61 34.483192977127416 38.0 33.0 40.0 22.0 41.0 62-63 34.2230267189644 37.5 33.0 40.0 22.0 41.0 64-65 34.01472664438822 37.0 33.0 40.0 21.0 41.0 66-67 33.774848934805476 36.5 32.5 40.0 21.0 41.0 68-69 33.50831300203225 36.0 32.0 39.0 21.0 41.0 70-71 33.23630407066534 35.5 32.0 39.0 20.0 41.0 72-73 32.90543531463679 35.0 32.0 39.0 20.0 40.5 74-75 32.574721368884596 35.0 31.0 37.5 20.0 40.0 76-77 32.18015542864124 35.0 31.0 37.0 19.5 39.5 78-79 31.809966849571445 35.0 31.0 37.0 18.5 39.0 80-81 31.50621905769892 35.0 31.0 36.0 18.0 39.0 82-83 31.204344745555527 35.0 30.0 36.0 18.0 37.5 84-85 30.926742021645712 35.0 30.0 35.0 17.0 37.0 86-87 30.697852368076187 34.0 30.0 35.0 17.0 37.0 88-89 30.48250060248329 34.0 30.0 35.0 16.0 36.0 90-91 30.300393365808546 34.0 29.5 35.0 15.0 36.0 92-93 30.13655220834966 34.0 29.0 35.0 15.0 36.0 94-95 29.974834411367176 34.0 29.0 35.0 14.5 35.0 96-97 29.864167047642226 34.0 29.0 35.0 13.0 35.0 98-99 29.731283047980575 34.0 29.0 35.0 11.0 35.0 100 29.63701853245178 34.0 29.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.102575151471985 1101 2 1.060953140748829 1101 3 1.0748896624287596 1101 4 1.0067086280418138 1101 5 1.0465133780701805 1101 6 1.014490663420105 1101 7 0.9976259434033281 1101 8 1.0412186730912154 1101 9 1.3194457376991693 1101 10-11 1.2246133977856815 1101 12-13 1.1764828425074896 1101 14-15 1.3567071865452007 1101 16-17 1.4027568791956355 1101 18-19 1.390673324087686 1101 20-21 1.3398025933455457 1101 22-23 1.313341338388426 1101 24-25 1.25889214787572 1101 26-27 1.3546355010137177 1101 28-29 1.267492189998201 1101 30-31 1.2116682633606146 1101 32-33 1.247865506256204 1101 34-35 1.2404547336787815 1101 36-37 1.2416980156534763 1101 38-39 1.2651782055427248 1101 40-41 1.1872444674849234 1101 42-43 1.314463909888218 1101 44-45 1.3228555092450875 1101 46-47 1.338882842486072 1101 48-49 1.3418188875683867 1101 50-51 1.2853643843893394 1101 52-53 1.2949041478897314 1101 54-55 1.3517638374828067 1101 56-57 1.323548715201909 1101 58-59 1.3589521334084083 1101 60-61 1.31398650214593 1101 62-63 1.2939565646746445 1101 64-65 1.2445152108018789 1101 66-67 1.235650097589783 1101 68-69 1.1780927748349157 1101 70-71 1.1277042130674246 1101 72-73 1.1159433249835544 1101 74-75 1.0273336556034778 1101 76-77 1.0339284392597108 1101 78-79 1.0891861114336479 1101 80-81 1.0495349579053546 1101 82-83 0.9822473458724481 1101 84-85 0.9588343383376774 1101 86-87 0.876910225184556 1101 88-89 0.9334580385240123 1101 90-91 0.8810727337478887 1101 92-93 0.8797102145235307 1101 94-95 0.8233706578777671 1101 96-97 0.8379930245175764 1101 98-99 0.8110271991325 1101 100 0.7304524083293842 1102 1 1.033865443915591 1102 2 1.029472350463081 1102 3 0.9833584516226281 1102 4 0.9596371324501547 1102 5 0.9932186473620703 1102 6 1.0325027049809492 1102 7 0.9914549634007059 1102 8 0.991684290711369 1102 9 1.1755670912805485 1102 10-11 1.137519179522208 1102 12-13 1.0797615837930152 1102 14-15 1.3446173661488885 1102 16-17 1.2996796268388309 1102 18-19 1.330927916665523 1102 20-21 1.2541696147949892 1102 22-23 1.2113004606190287 1102 24-25 1.1176129746107577 1102 26-27 1.2245407085922082 1102 28-29 1.161179795407044 1102 30-31 1.1795752968896167 1102 32-33 1.1472007412970129 1102 34-35 1.1499122276360652 1102 36-37 1.1566131702429274 1102 38-39 1.1127042626072239 1102 40-41 1.0989988985808097 1102 42-43 1.2135966090596142 1102 44-45 1.2724451001381567 1102 46-47 1.2267860450716768 1102 48-49 1.215037133588659 1102 50-51 1.1447312284438098 1102 52-53 1.2311025705118297 1102 54-55 1.3370293320916176 1102 56-57 1.2680934176473997 1102 58-59 1.2507549447438038 1102 60-61 1.2621546006621713 1102 62-63 1.224127249552211 1102 64-65 1.2055952742123424 1102 66-67 1.1652137031045342 1102 68-69 1.1114903822089488 1102 70-71 1.133046359253818 1102 72-73 1.0914547107440953 1102 74-75 0.9581064829872616 1102 76-77 0.9971780658050946 1102 78-79 1.0202277948579308 1102 80-81 1.0421137092735115 1102 82-83 1.02381819597802 1102 84-85 0.9720870663105643 1102 86-87 0.8943588282158963 1102 88-89 0.8819155767464792 1102 90-91 0.8814565097341927 1102 92-93 0.8907579540878565 1102 94-95 0.8436300012026621 1102 96-97 0.8794746518783647 1102 98-99 0.866864403221637 1102 100 0.8635617106803402 1103 1 0.9285655879349015 1103 2 0.9439614411138564 1103 3 0.9235799847661994 1103 4 0.8959260392093853 1103 5 0.9000898146228522 1103 6 0.965242922592175 1103 7 0.9076856657538528 1103 8 0.9144898756157716 1103 9 1.1445685441630857 1103 10-11 1.1301380529029146 1103 12-13 1.016136225165674 1103 14-15 1.2384101998473724 1103 16-17 1.2233167497028248 1103 18-19 1.2302403127185286 1103 20-21 1.1378646509853851 1103 22-23 1.1244324126096998 1103 24-25 1.1035307083790329 1103 26-27 1.1616709110960741 1103 28-29 1.0918548759154802 1103 30-31 1.090585227991447 1103 32-33 1.0908057410886371 1103 34-35 1.1246512484879219 1103 36-37 1.1108043155709169 1103 38-39 1.150033339848619 1103 40-41 1.0999809340058064 1103 42-43 1.16433964722804 1103 44-45 1.1444760200509378 1103 46-47 1.1489356794726504 1103 48-49 1.1575130792637296 1103 50-51 1.1285181633947232 1103 52-53 1.1183928328045667 1103 54-55 1.194762615575435 1103 56-57 1.2601314213224342 1103 58-59 1.273137988443878 1103 60-61 1.2346816645229381 1103 62-63 1.2017090459948463 1103 64-65 1.1986097473875148 1103 66-67 1.118840153722502 1103 68-69 1.078540932267039 1103 70-71 1.0624254282899699 1103 72-73 1.0956988924203799 1103 74-75 1.0480470551878298 1103 76-77 0.989390135609959 1103 78-79 1.0433459144576744 1103 80-81 0.9679900959739562 1103 82-83 0.9237431388554178 1103 84-85 0.8719100922158098 1103 86-87 0.8283693193572645 1103 88-89 0.8346715047087834 1103 90-91 0.8467467113075458 1103 92-93 0.8803915371278599 1103 94-95 0.9146740568668967 1103 96-97 0.8619667426889457 1103 98-99 0.8385228826169211 1103 100 0.744484257577458 1104 1 0.8922824296794296 1104 2 0.8517454324219287 1104 3 0.8875504468617734 1104 4 0.8816284777970083 1104 5 0.895872972004689 1104 6 0.9334058626422319 1104 7 0.9039249664199716 1104 8 0.8870247532728897 1104 9 1.1175128486539805 1104 10-11 1.0726698147717286 1104 12-13 1.0003553937819945 1104 14-15 1.1946017195743295 1104 16-17 1.215903482097339 1104 18-19 1.2069402726173735 1104 20-21 1.104938249706123 1104 22-23 1.1006081119565678 1104 24-25 0.9949095009955826 1104 26-27 1.119393823165204 1104 28-29 1.0426399844011556 1104 30-31 1.0338632700899524 1104 32-33 1.093160161124409 1104 34-35 1.0466522893340695 1104 36-37 1.0366124809903354 1104 38-39 1.0842638551382038 1104 40-41 1.0217723945825625 1104 42-43 1.0633617386337235 1104 44-45 1.0636500964174331 1104 46-47 1.073782624721666 1104 48-49 1.1350458922980167 1104 50-51 1.1172097811894233 1104 52-53 1.1465489495327432 1104 54-55 1.2066222593637335 1104 56-57 1.2061108529722375 1104 58-59 1.1920607933490146 1104 60-61 1.1948088673321138 1104 62-63 1.244040642673781 1104 64-65 1.1390410903861508 1104 66-67 1.0804886439546664 1104 68-69 1.088566901770676 1104 70-71 1.0927941719062702 1104 72-73 1.085528010716665 1104 74-75 1.01065015040448 1104 76-77 0.9906867824551497 1104 78-79 1.067653032480294 1104 80-81 1.0039436283094822 1104 82-83 1.0254583113351465 1104 84-85 0.9878616253709929 1104 86-87 0.9603120199277129 1104 88-89 0.9442130965700137 1104 90-91 0.8621277919602655 1104 92-93 0.9419819331950627 1104 94-95 0.9793853267698722 1104 96-97 0.9411373391145581 1104 98-99 0.9761841962166073 1104 100 0.9647728153265689 1105 1 0.6435957430299553 1105 2 0.6869404955396234 1105 3 0.718553169825249 1105 4 0.6973275955774341 1105 5 0.6695348786292996 1105 6 0.7578107959807454 1105 7 0.7663521538180973 1105 8 0.7433651200788347 1105 9 0.9457335394944977 1105 10-11 0.8767534826998329 1105 12-13 0.7726593303486524 1105 14-15 0.9487636653702438 1105 16-17 0.9204019870127027 1105 18-19 0.8783058205570526 1105 20-21 0.8870637601075515 1105 22-23 0.8726965813357381 1105 24-25 0.7735586985047718 1105 26-27 0.9160040986970941 1105 28-29 0.8796712437941636 1105 30-31 0.788706296465044 1105 32-33 0.784149898971215 1105 34-35 0.825986460337802 1105 36-37 0.8197149870726506 1105 38-39 0.8674361069945604 1105 40-41 0.8049984673323038 1105 42-43 0.8658046990783035 1105 44-45 0.8821879815223923 1105 46-47 0.9321324478027933 1105 48-49 0.9529159965756904 1105 50-51 0.8917238340315734 1105 52-53 0.9446122646088284 1105 54-55 1.0400432512518307 1105 56-57 0.9933267482233177 1105 58-59 0.9709148864865185 1105 60-61 1.063515610905995 1105 62-63 1.02946352494277 1105 64-65 0.930485149518411 1105 66-67 0.8648401024544938 1105 68-69 0.8554288848555345 1105 70-71 0.9153572964871657 1105 72-73 0.940264516900875 1105 74-75 0.8806537891421513 1105 76-77 0.8304578257580459 1105 78-79 0.8348103703191896 1105 80-81 0.9088565626592491 1105 82-83 0.874254876559224 1105 84-85 0.8240549913897119 1105 86-87 0.8379104338740397 1105 88-89 0.8124378711042759 1105 90-91 0.8403170128751398 1105 92-93 0.8369066242993561 1105 94-95 0.8673525906105937 1105 96-97 0.8109887018719846 1105 98-99 0.8626637054740449 1105 100 0.8468209926463786 1106 1 0.6682612413269062 1106 2 0.6794019989089186 1106 3 0.6445772003862373 1106 4 0.6865238924309551 1106 5 0.6981124745046614 1106 6 0.7837432342753843 1106 7 0.7449660408584222 1106 8 0.7421986346959883 1106 9 0.9000917838901685 1106 10-11 0.8580762164116251 1106 12-13 0.7611812570087082 1106 14-15 0.8119507217452977 1106 16-17 0.8514275273556109 1106 18-19 0.8671412473540201 1106 20-21 0.8447080486539136 1106 22-23 0.8318075485399774 1106 24-25 0.7182823279105222 1106 26-27 0.8342025688268748 1106 28-29 0.7920731946154049 1106 30-31 0.71453517516359 1106 32-33 0.7240623519796472 1106 34-35 0.6974722418381489 1106 36-37 0.7744468944782099 1106 38-39 0.7904760919124243 1106 40-41 0.7693592098946098 1106 42-43 0.6860877171359832 1106 44-45 0.6837706532745074 1106 46-47 0.7104631908825141 1106 48-49 0.7347503014559749 1106 50-51 0.7169606966179174 1106 52-53 0.7101910551811983 1106 54-55 0.8164927047290291 1106 56-57 0.8465924122487607 1106 58-59 0.8278496194203413 1106 60-61 0.8184460901465513 1106 62-63 0.7873938443240718 1106 64-65 0.8062169521530933 1106 66-67 0.7751800321412219 1106 68-69 0.7828391912543182 1106 70-71 0.7727455265816303 1106 72-73 0.7352997632822422 1106 74-75 0.7179742043680974 1106 76-77 0.7294863925449917 1106 78-79 0.7558369799008204 1106 80-81 0.7225830954845627 1106 82-83 0.6848701624792533 1106 84-85 0.6508656886538802 1106 86-87 0.5260354087607375 1106 88-89 0.6189041424498214 1106 90-91 0.6762661847908227 1106 92-93 0.6512948930305669 1106 94-95 0.65043553697382 1106 96-97 0.5840818540942045 1106 98-99 0.5992758784093262 1106 100 0.6666047461947393 1107 1 0.7624503686555997 1107 2 0.8354793263435596 1107 3 0.8132122058825573 1107 4 0.8350105675696113 1107 5 0.7535354988021012 1107 6 0.8110087347429484 1107 7 0.8217155308382686 1107 8 0.880611477945834 1107 9 1.0586596321860284 1107 10-11 0.9931731220926707 1107 12-13 0.9114858500267644 1107 14-15 1.153419998008708 1107 16-17 1.1626339054306385 1107 18-19 1.1765723439853701 1107 20-21 1.1108424136161759 1107 22-23 1.0706251135269653 1107 24-25 1.0001061985967468 1107 26-27 1.1268116806997952 1107 28-29 1.0791945261599452 1107 30-31 1.0245749714745003 1107 32-33 1.0232480163014799 1107 34-35 1.0678691764388972 1107 36-37 1.1070858148239182 1107 38-39 1.1083648353551823 1107 40-41 1.156315110093452 1107 42-43 1.128724711312202 1107 44-45 1.1112454026046095 1107 46-47 1.1364991765280266 1107 48-49 1.1373807754916712 1107 50-51 1.0969366740600606 1107 52-53 1.1414419800610247 1107 54-55 1.2120344676733126 1107 56-57 1.1646597749533854 1107 58-59 1.178892951518769 1107 60-61 1.1344804680997242 1107 62-63 1.1048819178489424 1107 64-65 1.0982060700114076 1107 66-67 1.0413011889430237 1107 68-69 1.0538021258972279 1107 70-71 1.0325297269652722 1107 72-73 1.0703252338001192 1107 74-75 0.9871357804615926 1107 76-77 1.025305126910915 1107 78-79 1.050312684316708 1107 80-81 1.08287121804997 1107 82-83 0.9993546988340576 1107 84-85 0.9726413991776148 1107 86-87 0.9131679818076641 1107 88-89 0.9057171491154747 1107 90-91 0.9460576681788346 1107 92-93 0.9160410497151013 1107 94-95 0.9451048374711242 1107 96-97 0.9598378726579178 1107 98-99 1.0061904170685274 1107 100 1.0201245224647977 1108 1 0.47168414259521185 1108 2 0.44310103178784743 1108 3 0.4447422372913721 1108 4 0.52790530364787 1108 5 0.5174654593613042 1108 6 0.5264055276290662 1108 7 0.5188537741343069 1108 8 0.5305607810064146 1108 9 0.7072210224200361 1108 10-11 0.6034030656578224 1108 12-13 0.5466373265216333 1108 14-15 0.6429932508361915 1108 16-17 0.6580399118105404 1108 18-19 0.6099819730443059 1108 20-21 0.5722022908147437 1108 22-23 0.5174041174914734 1108 24-25 0.46338735823264443 1108 26-27 0.5349833724147572 1108 28-29 0.49675970270714487 1108 30-31 0.5163768264834587 1108 32-33 0.5349729890314805 1108 34-35 0.5016839385021825 1108 36-37 0.5016465464207656 1108 38-39 0.45855682924148056 1108 40-41 0.5064203008157762 1108 42-43 0.5230073699936568 1108 44-45 0.4900479072733077 1108 46-47 0.5164674230099422 1108 48-49 0.5196143838000253 1108 50-51 0.35323947457616356 1108 52-53 0.38795641301246775 1108 54-55 0.5308540852939174 1108 56-57 0.5257407777429464 1108 58-59 0.5298143942984836 1108 60-61 0.46776289810870253 1108 62-63 0.3963913783221784 1108 64-65 0.3360916633577702 1108 66-67 0.4410041788899193 1108 68-69 0.40393752782244263 1108 70-71 0.498326969566385 1108 72-73 0.5020750757837149 1108 74-75 0.4335863457426896 1108 76-77 0.44220603714398266 1108 78-79 0.46769418840635524 1108 80-81 0.4716444694734143 1108 82-83 0.4672295355361378 1108 84-85 0.3928731883802037 1108 86-87 0.3788387594601552 1108 88-89 0.3113277962941794 1108 90-91 0.35754350137187885 1108 92-93 0.34556705111450725 1108 94-95 0.3530212718819783 1108 96-97 0.3488432976288074 1108 98-99 0.3687064754877518 1108 100 0.37336210858295615 1109 1 0.29074826676932375 1109 2 0.25270964471670254 1109 3 0.32007418967818424 1109 4 0.42431509868871586 1109 5 0.46539961385833806 1109 6 0.5012066645897946 1109 7 0.4500217728618736 1109 8 0.4849566197110846 1109 9 0.5502316286138935 1109 10-11 0.5063464625696881 1109 12-13 0.45188906741033463 1109 14-15 0.5665796282860001 1109 16-17 0.536674935877663 1109 18-19 0.5233133806048968 1109 20-21 0.46607699088195886 1109 22-23 0.47224739471378285 1109 24-25 0.3740721659811115 1109 26-27 0.4563306051588967 1109 28-29 0.44038068934640506 1109 30-31 0.3831602658815285 1109 32-33 0.31131210094623896 1109 34-35 0.3387813461597702 1109 36-37 0.3926834316679191 1109 38-39 0.31410153876274194 1109 40-41 0.3367998343214822 1109 42-43 0.3242712388298159 1109 44-45 0.39262977439344127 1109 46-47 0.41871040310055463 1109 48-49 0.377268261547556 1109 50-51 0.3075462657759189 1109 52-53 0.36254877614090475 1109 54-55 0.4321269305141584 1109 56-57 0.4246175671407926 1109 58-59 0.4821901781847089 1109 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-0.5227284524390505 2314 94-95 -0.5593913870606748 2314 96-97 -0.5988372207593535 2314 98-99 -0.554048140734416 2314 100 -0.5898269571013657 2315 1 -1.112412576714373 2315 2 -1.1431247054124007 2315 3 -1.2395079555368262 2315 4 -1.2160764500837828 2315 5 -1.1208282339242217 2315 6 -1.1532284226392093 2315 7 -1.0223717822586735 2315 8 -1.252848332668222 2315 9 -1.3564818864620207 2315 10-11 -1.320768877921516 2315 12-13 -1.4230711702485053 2315 14-15 -1.80154190576868 2315 16-17 -1.8263545852427612 2315 18-19 -1.99000288725086 2315 20-21 -2.0022939022693222 2315 22-23 -1.6371205255863472 2315 24-25 -1.5825017721825745 2315 26-27 -1.7121372021658345 2315 28-29 -1.669724886512192 2315 30-31 -1.6932483488652323 2315 32-33 -1.8362774592670874 2315 34-35 -1.8495313154364297 2315 36-37 -1.9057568353172414 2315 38-39 -1.8374656799464475 2315 40-41 -1.6705088978660498 2315 42-43 -1.6750074221019773 2315 44-45 -1.7619893861515692 2315 46-47 -1.7185972072297702 2315 48-49 -1.594799390007104 2315 50-51 -1.499776332682245 2315 52-53 -1.8601970132727317 2315 54-55 -2.0495217782109165 2315 56-57 -1.9926776515398998 2315 58-59 -2.2302589101211936 2315 60-61 -1.9944014533327135 2315 62-63 -1.949347315775249 2315 64-65 -1.839755100254088 2315 66-67 -1.6870755010248857 2315 68-69 -1.9689565590765206 2315 70-71 -1.9389030286323177 2315 72-73 -1.9321283126536066 2315 74-75 -2.068698767068881 2315 76-77 -1.8984984904824707 2315 78-79 -1.7405397441570365 2315 80-81 -1.9199265484965515 2315 82-83 -1.7572440981175426 2315 84-85 -1.4947527709008916 2315 86-87 -1.4654107957072178 2315 88-89 -1.41942612072069 2315 90-91 -1.542458948747452 2315 92-93 -1.3655845593166411 2315 94-95 -1.4635996792001187 2315 96-97 -1.2862535501625239 2315 98-99 -1.2948939487377409 2315 100 -1.2298308160368023 2316 1 -4.536217136931846 2316 2 -3.32382407702913 2316 3 -4.116657428474436 2316 4 -3.4233442238775815 2316 5 -2.844509118388764 2316 6 -5.5348426042253145 2316 7 -5.606849025265806 2316 8 -5.420957966261263 2316 9 -4.0545669966677735 2316 10-11 -5.20095562895694 2316 12-13 -4.183086296400347 2316 14-15 -4.275828678954035 2316 16-17 -5.51397703495935 2316 18-19 -3.965774017235198 2316 20-21 -3.0406900990802797 2316 22-23 -3.766075208315165 2316 24-25 -2.9037182228003715 2316 26-27 -3.7706552661364583 2316 28-29 -3.2220039609584106 2316 30-31 -3.0536980304341768 2316 32-33 -2.79983579364157 2316 34-35 -2.701220180126491 2316 36-37 -2.709174667943735 2316 38-39 -2.9195913434808034 2316 40-41 -2.8442957354824827 2316 42-43 -2.9971581674900776 2316 44-45 -2.923018422009406 2316 46-47 -3.290615695338083 2316 48-49 -3.142806886511032 2316 50-51 -2.9332651993826886 2316 52-53 -3.0251209116373587 2316 54-55 -3.090570542725878 2316 56-57 -3.1266222179775767 2316 58-59 -3.3527217764871864 2316 60-61 -3.4535409241085375 2316 62-63 -3.1079192746279176 2316 64-65 -2.841499728448163 2316 66-67 -2.986697489401781 2316 68-69 -3.0020589394647814 2316 70-71 -2.8887381076742784 2316 72-73 -2.984336091377237 2316 74-75 -2.6632517011965504 2316 76-77 -2.6350649326642426 2316 78-79 -5.678858860207974 2316 80-81 -3.2176583120577327 2316 82-83 -2.4113199479898846 2316 84-85 -2.2491838530839345 2316 86-87 -2.3777952347413738 2316 88-89 -2.2107252086610636 2316 90-91 -2.2543207626939115 2316 92-93 -2.2686906875078954 2316 94-95 -2.2543431877924007 2316 96-97 -2.2297598992660532 2316 98-99 -2.298174025472047 2316 100 -2.352379641507504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 77.0 5 230.0 6 916.0 7 7995.0 8 12218.0 9 23044.0 10 31888.0 11 37418.0 12 45202.0 13 56746.0 14 73338.0 15 94401.0 16 117665.0 17 141867.0 18 163736.0 19 187564.0 20 217517.0 21 265416.0 22 336274.0 23 442491.0 24 578382.0 25 673175.0 26 663067.0 27 605933.0 28 586085.0 29 630345.0 30 721030.0 31 854075.0 32 1035125.0 33 1293062.0 34 1687312.0 35 2342634.0 36 3488405.0 37 5671702.0 38 6769067.0 39 2095667.0 40 4161.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.911838970877394 35.69240297551581 27.430100478849656 18.965657574757138 2 20.749971229489645 33.90788911023742 26.588439672488466 18.753699987784465 3 25.994385514661627 26.47850758326052 26.776415856841794 20.75069104523606 4 20.033894358878392 27.105867376077175 26.22743362028742 26.632804644757012 5 20.40038573962563 32.93808770106331 26.35206489177954 20.309461667531522 6 26.867409520246806 26.679064840783717 26.247727172658337 20.205798466311148 7 20.3821983140607 26.77507412476882 32.5960187503503 20.246708810820184 8 26.525512573946003 26.96510240901626 26.299130317988816 20.210254699048917 9 26.427272891423048 26.779162532883156 26.509772794646903 20.28379178104689 10-11 19.983997618168637 30.285130361862684 29.613184290290018 20.117687729678664 12-13 19.98781858739112 27.04618612976907 29.674649947115096 23.29134533572472 14-15 19.96884361042099 33.418719425791885 26.487027496551974 20.12540946723516 16-17 23.24500708694345 26.969042294561802 26.43466519719471 23.351285421300037 18-19 20.06685413094809 30.15732347896713 26.424602685700123 23.35121970438466 20-21 23.278047591898147 30.201056859496816 26.39209594414849 20.128799604456546 22-23 23.31244064238358 27.085001821293233 29.47960117913764 20.12295635718555 24-25 20.14099153373276 30.21995788845136 26.23897101596835 23.400079561847534 26-27 20.181240657639567 27.1028808302911 32.532559209070435 20.183319302998907 28-29 20.179996532285177 27.153404494063864 32.52474980885228 20.14184916479868 30-31 20.181629468721408 27.11832073255833 32.52667211693646 20.173377681783798 32-33 20.212988385994773 27.14389810731982 32.477337020337 20.1657764863484 34-35 20.219024963751913 27.237149256962766 32.40875149434525 20.135074284940067 36-37 20.223982313125973 27.200121064751848 32.37128617195693 20.204610450165248 38-39 20.245314236304594 27.18640363040673 32.33390600686648 20.234376126422195 40-41 20.264554234629625 27.2696213807989 32.293301717795245 20.17252266677623 42-43 20.257287418312742 27.315198698854616 32.28186041890703 20.14565346392562 44-45 20.31041343880475 27.334259762980583 32.206330727867396 20.148996070347266 46-47 20.28807613191272 27.401258011290164 32.15377450351134 20.156891353285765 48-49 20.374467362861022 27.412516535299382 32.0086426501506 20.204373451689005 50-51 20.38388677096338 27.49564164462475 31.93215914449595 20.18831243991592 52-53 20.442397098099416 27.691961675279124 31.712623442839096 20.153017783782364 54-55 20.800883167979322 27.918993581662637 30.945391818143897 20.33473143221414 56-57 21.20224392304232 28.467639927741423 29.88957072997742 20.44054541923883 58-59 21.32374691049828 28.933174038233766 29.29273533016319 20.450343721104765 60-61 21.36721410616322 29.24657238590424 28.917558821150042 20.468654686782504 62-63 21.421174120243013 29.38845766854092 28.7668253525876 20.423542858628473 64-65 21.486466686510735 29.426968391493567 28.771334099702123 20.31523082229358 66-67 21.58447635143654 29.227242718618722 28.911089297225967 20.27719163271877 68-69 21.653091736933515 29.016418371217906 29.105768943696724 20.22472094815185 70-71 21.72302106236798 28.901482250134485 29.274444852892827 20.101051834604707 72-73 21.85674605054559 28.844255081285187 29.250634840680267 20.048364027488958 74-75 22.02646016500373 28.901387009856204 29.093434094900367 19.978718730239695 76-77 22.275693616259698 29.02630073488586 28.77049073307517 19.92751491577927 78-79 22.504069908297353 29.134782868069724 28.42604848381231 19.93509873982061 80-81 22.703813174454496 29.267777169977972 28.110335685031785 19.918073970535747 82-83 22.8805940166136 29.407415922482016 27.84844194013596 19.863548120768428 84-85 23.03896650674739 29.44853280228993 27.66125519149556 19.85124549946712 86-87 23.23907583874608 29.446466623355622 27.497685529703297 19.816772008195002 88-89 23.43456611559476 29.465953944141866 27.364030903138154 19.73544903712522 90-91 23.622881883297108 29.41910151884304 27.189258215527335 19.76875838233252 92-93 23.77845572055744 29.40513988566827 27.10692555134102 19.709478842433274 94-95 23.93811037891173 29.40347368068888 27.04919558549981 19.609220354899584 96-97 24.091299116865418 29.388392407136273 26.924994700447336 19.59531377555098 98-99 24.225681114019686 29.36155140108473 26.839631293022965 19.573136191872617 100 24.302040533134555 29.43173671997812 26.713441929992776 19.55278081689455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 90408.0 1 56670.0 2 18887.5 3 14071.5 4 13123.0 5 13061.0 6 13488.5 7 13822.5 8 13911.0 9 14120.0 10 15485.5 11 19121.0 12 118256.0 13 148025.0 14 80904.5 15 86720.0 16 98579.0 17 110621.0 18 122794.0 19 134397.0 20 148866.5 21 171543.0 22 203282.5 23 238054.0 24 270760.0 25 306226.0 26 345625.0 27 392718.0 28 457406.0 29 520207.0 30 567629.5 31 621281.5 32 680568.5 33 726166.5 34 762615.5 35 815745.0 36 874369.5 37 909863.5 38 942934.5 39 970349.0 40 980838.5 41 1001289.5 42 1036217.5 43 1043982.5 44 1032328.0 45 1033966.0 46 1036801.0 47 1034126.0 48 1023286.5 49 1008662.5 50 992922.5 51 973476.5 52 944633.5 53 900222.5 54 843785.0 55 781943.0 56 716692.5 57 648960.0 58 576344.0 59 494896.0 60 409898.5 61 333134.5 62 263710.0 63 199029.5 64 146387.5 65 106022.0 66 75887.5 67 55654.0 68 41341.0 69 30706.0 70 23272.5 71 18482.0 72 15277.5 73 12319.5 74 10249.5 75 9480.5 76 7343.5 77 4185.0 78 2397.5 79 1664.5 80 1214.0 81 951.5 82 731.0 83 557.0 84 462.0 85 390.5 86 332.0 87 267.0 88 196.0 89 171.5 90 145.0 91 111.5 92 93.5 93 90.5 94 73.5 95 49.5 96 34.0 97 25.0 98 23.0 99 17.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03631641918667306 2 0.012905550428766885 3 0.0034798671379216235 4 2.2844451534917136E-4 5 2.8164392303322494E-5 6 0.0 7 0.0 8 0.0 9 1.6898635381993495E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.05448871097616125 22-23 0.07279713066178217 24-25 0.04791389006179675 26-27 0.03555598059448335 28-29 0.03760415779031942 30-31 0.03331847609483051 32-33 0.034316747333137165 34-35 0.03594715270980728 36-37 0.0375728640210935 38-39 0.05148607381893481 40-41 0.07280495410408865 42-43 0.08662428259425221 44-45 0.08203035727188805 46-47 0.049891656276874495 48-49 0.03113417100286172 50-51 0.019605546420035046 52-53 0.029050005972415856 54-55 0.02780607864568578 56-57 0.028597811007101404 58-59 0.034973916486881355 60-61 0.06333076547094323 62-63 0.09394545990465476 64-65 0.11910252098536694 66-67 0.09264207441639546 68-69 0.05671213327966243 70-71 0.05840356150632308 72-73 0.05786217929871477 74-75 0.04825342745789791 76-77 0.05346853409939646 78-79 0.04318070746637727 80-81 0.038051658690249984 82-83 0.037220809117301974 84-85 0.05040956815756338 86-87 0.05166444830352252 88-89 0.06068644197135349 90-91 0.05253597977646423 92-93 0.048948149134713195 94-95 0.05460293323383584 96-97 0.05077883463442916 98-99 0.042276317535748356 100 0.05050501415370241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1955243E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.76507798198513 #Duplication Level Percentage of deduplicated Percentage of total 1 77.48021667864697 34.684079416807364 2 10.918392586208352 9.775253911190903 3 3.9382742272097886 5.288914586864651 4 2.0701576524505936 3.7068307498781627 5 1.2134385884150645 2.715983651837516 6 0.8220894306527439 2.20805384828015 7 0.5773139220016305 1.8090451916942067 8 0.43800956159631715 1.5686025745371406 9 0.3348774607505599 1.349173408041718 >10 1.9134209877567434 15.684639798311526 >50 0.17148066426413852 5.3142716926674165 >100 0.12150710716731702 9.432384616310108 >500 3.2021405418198017E-4 0.09393622566834048 >1k 4.1780439199462987E-4 0.4071007141085577 >5k 4.84834195947362E-5 0.14635384947241362 >10k+ 3.463101392182992E-5 5.8153757643298105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1690120 5.289022524410157 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126756 0.39666730119999394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05894807309085398 6.2587538451827765E-6 0.0 0.0 6.2587538451827765E-6 2 0.058960590598544346 1.564688461295694E-5 0.0 3.1293769225913882E-6 1.2517507690365553E-5 3 0.0589762374831573 2.1905638458139718E-5 0.0 3.1293769225913882E-6 2.5035015380731106E-5 4 0.058979366860079895 2.8164392303322494E-5 0.0 3.1293769225913882E-6 2.8164392303322494E-5 5 0.05899188436777026 3.442314614850527E-5 0.0 6.2587538451827765E-6 2.8164392303322494E-5 6 0.05902630751391876 3.442314614850527E-5 0.0 9.388130767774165E-6 2.8164392303322494E-5 7 0.05902630751391876 3.442314614850527E-5 0.0 9.388130767774165E-6 2.8164392303322494E-5 8 0.05903569564468654 4.068189999368805E-5 0.0 9.388130767774165E-6 3.755252307109666E-5 9 0.059048213152376905 4.068189999368805E-5 0.0 9.388130767774165E-6 3.755252307109666E-5 10-11 0.059063860036989865 4.694065383887082E-5 0.0 9.388130767774165E-6 4.3811276916279435E-5 12-13 0.05907481285621893 5.789347306794068E-5 0.0 1.0952819229069859E-5 5.007003076146221E-5 14-15 0.05909828318313837 5.9458161529236376E-5 0.0 1.877626153554833E-5 5.632878460664499E-5 16-17 0.0591201888215965 6.258753845182776E-5 0.0 2.65997038420268E-5 6.571691537441915E-5 18-19 0.05914365914851594 6.884629229701053E-5 0.0 3.129376922591388E-5 7.82344230647847E-5 20-21 0.059176517606203155 7.82344230647847E-5 0.0 3.285845768720957E-5 9.388130767774165E-5 22-23 0.05922502294850332 8.449317690996749E-5 0.0 3.442314614850527E-5 1.0796350382940289E-4 24-25 0.05940965618693621 8.762255383255887E-5 0.0 4.068189999368805E-5 1.1578694613588136E-4 26-27 0.060051178456067444 1.0014006152292442E-4 0.0 4.068189999368805E-5 1.2517507690365553E-4 28-29 0.062201060401887734 1.1109288075199429E-4 0.0 4.068189999368805E-5 1.3925727305531677E-4 30-31 0.0680639480663627 1.1891632305847275E-4 0.0 4.3811276916279435E-5 1.4864540382309094E-4 32-33 0.08062996109902841 1.1891632305847275E-4 0.0 4.3811276916279435E-5 1.595982230521608E-4 34-35 0.10267172745330086 1.2517507690365553E-4 0.0 5.16347192227579E-5 1.658569768973436E-4 36-37 0.13861105672080165 1.2673976536495122E-4 0.0 5.31994076840536E-5 1.7680979612641343E-4 38-39 0.19217973088172102 1.314338307488383E-4 0.0 5.31994076840536E-5 1.8463323843289188E-4 40-41 0.26505822534349055 1.4551602690049956E-4 0.0 5.31994076840536E-5 1.9558605766196177E-4 42-43 0.359239953205801 1.5021009228438663E-4 0.0 5.47640961453493E-5 2.0340949996844023E-4 44-45 0.4766839044221945 1.564688461295694E-4 0.0 5.632878460664499E-5 2.2062107304269288E-4 46-47 0.6213862933228202 1.7837448458770912E-4 0.0 5.789347306794068E-5 2.362679576556498E-4 48-49 0.7944533546498145 1.830685499715962E-4 0.0 5.9458161529236376E-5 2.4409139996212827E-4 50-51 0.9962966014684976 1.8932730381677898E-4 0.0 6.258753845182776E-5 2.4409139996212827E-4 52-53 1.2275137447710849 2.0184481150714454E-4 0.0 6.258753845182776E-5 2.5035015380731106E-4 54-55 1.4877918468653173 2.065388768910316E-4 0.0 6.258753845182776E-5 2.597382845750852E-4 56-57 1.7782105427894885 2.112329422749187E-4 0.0 6.884629229701054E-5 2.691264153428594E-4 58-59 2.101713011539296 2.1749169612010147E-4 0.0 7.041098075830623E-5 2.7694985764933785E-4 60-61 2.4547114224729882 2.2375044996528426E-4 0.0 7.197566921960192E-5 2.8946736533970343E-4 62-63 2.8355253001831344 2.2844451534917133E-4 0.0 7.354035768089763E-5 3.0042018456877327E-4 64-65 3.24500739988114 2.3000920381046702E-4 0.0 7.82344230647847E-5 3.129376922591388E-4 66-67 3.6846473049821586 2.393973345782412E-4 0.0 7.979911152608039E-5 3.22325823026913E-4 68-69 4.151877361721205 2.409620230395369E-4 0.0 8.762255383255887E-5 3.3014926533339145E-4 70-71 4.646974519955927 2.5191484226860675E-4 0.0 9.388130767774165E-5 3.379727076398699E-4 72-73 5.166355643109958 2.597382845750852E-4 0.0 9.388130767774165E-5 3.457961499463484E-4 74-75 5.71034775107171 2.8007923457192924E-4 0.0 9.857537306162873E-5 3.5361959225282686E-4 76-77 6.280215112117908 2.8946736533970343E-4 0.0 1.032694384455158E-4 3.5831365763671393E-4 78-79 6.874236568941128 2.972908076461819E-4 0.0 1.0639881536810719E-4 3.63007723020601E-4 80-81 7.49445403998336 3.0667893841395603E-4 0.0 1.1422225767458567E-4 3.708311653270795E-4 82-83 8.139002416598741 3.098083153365474E-4 0.0 1.1891632305847275E-4 3.739605422496709E-4 84-85 8.805005801395406 3.113730037978431E-4 0.0 1.1891632305847275E-4 3.8021929609485366E-4 86-87 9.489636802323801 3.254551999495044E-4 0.0 1.1891632305847275E-4 3.8334867301744504E-4 88 10.015580228884506 3.3171395379468714E-4 0.0 1.1891632305847275E-4 3.974308691691063E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 211200 0.0 88.027954 8 GTGGTAT 213160 0.0 87.08194 6 TGGTATC 213600 0.0 86.66068 7 GTATCAA 215300 0.0 86.35599 9 AGTGGTA 215085 0.0 86.15407 5 GCAGTGG 225055 0.0 83.21223 3 AAGCAGT 226425 0.0 82.83579 1 CAGTGGT 226800 0.0 81.72048 4 AGCAGTG 234475 0.0 80.117714 2 CAACGCA 209425 0.0 45.57445 12-13 ATCAACG 211075 0.0 45.29051 10-11 TCAACGC 209410 0.0 45.08429 12-13 ACGCAGA 212735 0.0 44.849255 14-15 AACGCAG 211550 0.0 44.673965 14-15 AGAGTAC 211980 0.0 44.296535 18-19 CGCAGAG 213880 0.0 44.056847 16-17 GAGTACT 208700 0.0 43.99427 20-21 TATCAAC 215020 0.0 43.418724 10-11 AGTACTT 220080 0.0 42.300674 20-21 GTACTTT 220340 0.0 41.731613 22-23 >>END_MODULE