FastQCFastQC Report
Thu 26 May 2016
SRR1660769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31955243
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20034166.269443796750348No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA833500.2608335664979922No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418620.1310019767335207No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT350150.10957513294453747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2430600.088.75718
GTGGTAT2468150.087.730466
TGGTATC2466550.087.47687
GTATCAA2468450.087.3542569
AGTGGTA2484450.087.313785
GCAGTGG2576550.084.44993
AAGCAGT2525750.083.987611
CAGTGGT2604550.083.256934
AGCAGTG2655200.081.760772
CAACGCA2392200.045.73242612-13
ATCAACG2405350.045.4764810-11
TCAACGC2384500.045.22871812-13
ACGCAGA2431400.045.1422414-15
AACGCAG2412700.044.86675314-15
AGAGTAC2449950.044.4800418-19
CGCAGAG2446550.044.27480316-17
GAGTACT2415650.044.12935620-21
TATCAAC2457750.043.8452910-11
AGTACTT2532750.042.68195720-21
GTACTTT2526100.042.1640722-23
Produced by FastQC (version 0.11.5)