##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660769_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31955243 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.169556463707693 31.0 31.0 34.0 25.0 34.0 2 31.226793237028428 31.0 31.0 34.0 27.0 34.0 3 31.868630540534458 33.0 31.0 34.0 30.0 34.0 4 35.68434898148013 37.0 35.0 37.0 33.0 37.0 5 35.63353747615063 37.0 35.0 37.0 33.0 37.0 6 35.680537744619876 37.0 35.0 37.0 33.0 37.0 7 35.59299852609476 37.0 35.0 37.0 33.0 37.0 8 35.60499496123375 37.0 35.0 37.0 33.0 37.0 9 37.270548779741716 39.0 37.0 39.0 33.0 39.0 10-11 37.14734464701145 39.0 37.0 39.0 33.0 39.0 12-13 37.240627242296355 39.0 37.0 39.0 33.0 39.0 14-15 38.502375666490785 40.0 38.0 41.0 33.5 41.0 16-17 38.49047337865652 40.0 38.0 41.0 33.0 41.0 18-19 38.45839557533642 40.0 38.0 41.0 33.5 41.0 20-21 38.3728651195048 40.0 38.0 41.0 33.5 41.0 22-23 38.21935023307443 40.0 37.5 41.0 33.5 41.0 24-25 38.232870596540295 40.0 38.0 41.0 33.0 41.0 26-27 38.180272561219454 40.0 38.0 41.0 33.0 41.0 28-29 38.076352509664844 40.0 37.5 41.0 33.0 41.0 30-31 38.01039708882827 40.0 37.0 41.0 33.0 41.0 32-33 37.90332991678392 40.0 37.0 41.0 33.0 41.0 34-35 37.79654288656168 40.0 37.0 41.0 32.0 41.0 36-37 37.605296382818935 39.5 37.0 41.0 32.0 41.0 38-39 37.151056932973404 39.0 35.0 41.0 31.0 41.0 40-41 36.938522279426884 39.0 35.0 41.0 31.0 41.0 42-43 36.82130755507007 39.0 35.0 40.0 31.0 41.0 44-45 36.717738291021604 39.0 35.0 40.0 30.5 41.0 46-47 36.81615794628756 39.0 35.0 40.5 31.0 41.0 48-49 36.77250321645184 39.0 35.0 41.0 31.0 41.0 50-51 36.610252721282706 39.0 35.0 41.0 30.0 41.0 52-53 36.347290286604924 39.0 35.0 40.5 30.0 41.0 54-55 35.91739250738917 39.0 35.0 41.0 27.5 41.0 56-57 35.42425354111687 39.0 34.5 41.0 25.5 41.0 58-59 35.196857820796424 38.0 34.0 40.0 25.0 41.0 60-61 35.03321445560593 38.0 34.0 40.0 25.0 41.0 62-63 34.79273845609623 38.0 34.0 40.0 25.0 41.0 64-65 34.54417289206657 37.0 34.0 40.0 24.0 41.0 66-67 34.31584335002553 37.0 33.5 40.0 24.0 41.0 68-69 34.08763211407906 36.0 33.0 39.0 24.0 41.0 70-71 33.78671927170136 36.0 33.0 39.0 24.0 41.0 72-73 33.46940525534417 35.0 33.0 39.0 24.0 40.5 74-75 33.11183219604996 35.0 32.5 37.5 23.5 40.0 76-77 31.03521127972646 33.5 30.0 35.5 20.0 38.5 78-79 32.300717757020344 35.0 31.5 37.0 22.0 39.0 80-81 32.307256308456175 35.0 32.0 36.0 22.0 39.0 82-83 32.10549176546709 35.0 32.0 36.0 21.5 37.5 84-85 31.861832798455016 35.0 32.0 35.5 20.0 37.0 86-87 31.6518067942716 35.0 32.0 35.0 20.0 37.0 88-89 31.454216574100222 35.0 32.0 35.0 20.0 36.0 90-91 31.29827670219876 35.0 32.0 35.0 20.0 36.0 92-93 31.163388195796227 35.0 32.0 35.0 19.0 36.0 94-95 31.034422238629197 35.0 31.0 35.0 19.0 35.5 96-97 30.934075716463806 35.0 31.0 35.0 19.0 35.0 98-99 30.81525790619086 34.0 31.0 35.0 18.0 35.0 100 30.671994983733967 34.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.382415492311104 1101 2 1.54994882198271 1101 3 1.0046839792235254 1101 4 0.635361705759756 1101 5 0.6509243380825609 1101 6 0.5321759100845895 1101 7 0.541961792810568 1101 8 0.5738855311859012 1101 9 0.6935572805933958 1101 10-11 0.7255610435817985 1101 12-13 0.7031217466981943 1101 14-15 0.9299019721052275 1101 16-17 0.9131392923570729 1101 18-19 0.8868492479948102 1101 20-21 0.8981480302066629 1101 22-23 0.9194870668630699 1101 24-25 0.8761595226609344 1101 26-27 0.8898003022677798 1101 28-29 0.9220063357577502 1101 30-31 0.8470944949487631 1101 32-33 0.8735339959582475 1101 34-35 0.8742446787112144 1101 36-37 0.9473797168598352 1101 38-39 1.0248684509261992 1101 40-41 1.1306828386930476 1101 42-43 1.0577113617052234 1101 44-45 1.0268746491143688 1101 46-47 0.9912158215188853 1101 48-49 0.9237768597104079 1101 50-51 0.9372816160942605 1101 52-53 1.0181652142956352 1101 54-55 1.1013191557746111 1101 56-57 1.0998528991890453 1101 58-59 1.0832922854602032 1101 60-61 1.0743659979329507 1101 62-63 1.0802703434363323 1101 64-65 1.0390805300169532 1101 66-67 1.0022517680510603 1101 68-69 0.9692660704060998 1101 70-71 0.9717650929002062 1101 72-73 0.9665058847680825 1101 74-75 0.8795707418495553 1101 76-77 1.1804515398042525 1101 78-79 1.0071510326671103 1101 80-81 0.9395583937094116 1101 82-83 0.9051106503603066 1101 84-85 0.8676122329250759 1101 86-87 0.8565105905250654 1101 88-89 0.8854379910391827 1101 90-91 0.8702427196808955 1101 92-93 0.8586008890762145 1101 94-95 0.8618878452093419 1101 96-97 0.8076326783993366 1101 98-99 0.8083399082220311 1101 100 0.6928758272091002 1102 1 2.364492400120561 1102 2 1.5423215988125065 1102 3 0.9791733998580092 1102 4 0.6009880798694098 1102 5 0.5760212182971998 1102 6 0.5388766731145438 1102 7 0.4952592286128734 1102 8 0.5541605490155277 1102 9 0.6323902876799607 1102 10-11 0.703349448344774 1102 12-13 0.6142105192942964 1102 14-15 0.8254426953643161 1102 16-17 0.8317451934161824 1102 18-19 0.8060812912130899 1102 20-21 0.7837613651893207 1102 22-23 0.7939730028682703 1102 24-25 0.778770203571348 1102 26-27 0.8282556257988318 1102 28-29 0.8561007615470686 1102 30-31 0.8775676412304421 1102 32-33 0.8293705249676151 1102 34-35 0.8064087309031507 1102 36-37 0.8263840489261227 1102 38-39 0.992341770472386 1102 40-41 1.0105391727642044 1102 42-43 0.9277399566757296 1102 44-45 0.9151138868326711 1102 46-47 0.8777663659889186 1102 48-49 0.8360581550537134 1102 50-51 0.8805334590023506 1102 52-53 0.9368991632444192 1102 54-55 1.0630037205907357 1102 56-57 1.0849329629337632 1102 58-59 0.9860179275016492 1102 60-61 0.9244683480247105 1102 62-63 0.9411722066779902 1102 64-65 0.9608631798054503 1102 66-67 0.9413483176855166 1102 68-69 0.8962790944199099 1102 70-71 0.8606926344334838 1102 72-73 0.8460196095875219 1102 74-75 0.8309532031553033 1102 76-77 0.7603046858992286 1102 78-79 0.9349293261556895 1102 80-81 0.8453339667757405 1102 82-83 0.8548470570769737 1102 84-85 0.8017080088739448 1102 86-87 0.8202399278015768 1102 88-89 0.8070075734750617 1102 90-91 0.7825204736906102 1102 92-93 0.7546821232218335 1102 94-95 0.7597123979573546 1102 96-97 0.7793174541555068 1102 98-99 0.8023600856222544 1102 100 0.6615472611446691 1103 1 2.0318951436886863 1103 2 1.271629439982668 1103 3 0.7214414814899008 1103 4 0.5286529261752548 1103 5 0.4643566051813508 1103 6 0.4558275088386452 1103 7 0.4925629319429774 1103 8 0.5261430176855058 1103 9 0.6195018486117903 1103 10-11 0.5882958853490976 1103 12-13 0.6030457690500768 1103 14-15 0.7953487013760494 1103 16-17 0.7654780889402559 1103 18-19 0.7560456351406089 1103 20-21 0.7205809548759561 1103 22-23 0.7500598227956985 1103 24-25 0.7715423355738196 1103 26-27 0.7196907734870948 1103 28-29 0.8280802068216104 1103 30-31 0.8170436689495233 1103 32-33 0.7861989756383423 1103 34-35 0.772651419280372 1103 36-37 0.7880336610050591 1103 38-39 0.8895184259461146 1103 40-41 0.9221066750758098 1103 42-43 0.860087350375025 1103 44-45 0.8493582765217269 1103 46-47 0.842161595504102 1103 48-49 0.7989377827196549 1103 50-51 0.7955358392777399 1103 52-53 0.8368828221829148 1103 54-55 0.9824135566571286 1103 56-57 0.9393497934595274 1103 58-59 0.9325351022131798 1103 60-61 0.9093171172358367 1103 62-63 0.8887842798601255 1103 64-65 0.8919298931054342 1103 66-67 0.8915457598501604 1103 68-69 0.7838834947477835 1103 70-71 0.7930315713480027 1103 72-73 0.7750733928798539 1103 74-75 0.8074382807465099 1103 76-77 0.7441470053990855 1103 78-79 0.8205015199228924 1103 80-81 0.7538304458323921 1103 82-83 0.7788443944545165 1103 84-85 0.7666444845096336 1103 86-87 0.7235183633462121 1103 88-89 0.7283002944645602 1103 90-91 0.7933999542851922 1103 92-93 0.7755849764075791 1103 94-95 0.7821216123223422 1103 96-97 0.7433750092554234 1103 98-99 0.7873359575163974 1103 100 0.6078778541833216 1104 1 2.1907411803504644 1104 2 1.425591360546754 1104 3 0.8629889290797941 1104 4 0.5448429022486039 1104 5 0.46964776082165827 1104 6 0.4440163357011784 1104 7 0.4498402541974613 1104 8 0.4942497817238376 1104 9 0.5388247413274314 1104 10-11 0.5744004112902346 1104 12-13 0.5482484569069541 1104 14-15 0.6917486355892137 1104 16-17 0.6986021801148894 1104 18-19 0.6078922974412109 1104 20-21 0.6742819000348206 1104 22-23 0.6773931461858993 1104 24-25 0.7239944554468778 1104 26-27 0.6726412622567963 1104 28-29 0.7379940719912597 1104 30-31 0.7640494075217106 1104 32-33 0.7636537142247235 1104 34-35 0.6425527667240303 1104 36-37 0.6990082701453844 1104 38-39 0.7673098058669936 1104 40-41 0.8704666856269085 1104 42-43 0.8392958865960125 1104 44-45 0.7516210751389778 1104 46-47 0.8364379332154002 1104 48-49 0.7884723164340386 1104 50-51 0.7871771270375447 1104 52-53 0.8413220812939528 1104 54-55 0.9962091271678801 1104 56-57 1.0250091336547982 1104 58-59 0.9393359651009519 1104 60-61 0.82361760110647 1104 62-63 0.8593932983792101 1104 64-65 0.867874641766889 1104 66-67 0.8839180248553262 1104 68-69 0.8508214710098372 1104 70-71 0.8003180693128584 1104 72-73 0.7502901724108213 1104 74-75 0.7461598123234481 1104 76-77 0.6292282140786725 1104 78-79 0.8213727633242414 1104 80-81 0.7589874272826478 1104 82-83 0.7629711243411066 1104 84-85 0.7087115256999681 1104 86-87 0.7776841799093042 1104 88-89 0.7918244779725327 1104 90-91 0.7885631327934135 1104 92-93 0.7725447024494727 1104 94-95 0.7658605450010754 1104 96-97 0.7662692829292475 1104 98-99 0.8027814624957941 1104 100 0.6358783676365292 1105 1 2.2279994243770034 1105 2 1.404831745160351 1105 3 0.8452788057629412 1105 4 0.5076367545454303 1105 5 0.4442719793165182 1105 6 0.3837743863427292 1105 7 0.37909701627251735 1105 8 0.4125098308443569 1105 9 0.48941664387573525 1105 10-11 0.47409019891772886 1105 12-13 0.4710094701686742 1105 14-15 0.5978966907746468 1105 16-17 0.5901448348211744 1105 18-19 0.5482411591538607 1105 20-21 0.46527323127025255 1105 22-23 0.5721020589096355 1105 24-25 0.6259551940950132 1105 26-27 0.6534776936439997 1105 28-29 0.6181348013273364 1105 30-31 0.6251471160393578 1105 32-33 0.6184110543354251 1105 34-35 0.5995312393452821 1105 36-37 0.6898216512709539 1105 38-39 0.7771471860088468 1105 40-41 0.7927313849486595 1105 42-43 0.7060701271406558 1105 44-45 0.7192290168568363 1105 46-47 0.7191668053750462 1105 48-49 0.6823664766413131 1105 50-51 0.7120811469363062 1105 52-53 0.8178239492839623 1105 54-55 0.8847985565336884 1105 56-57 0.8284468578044937 1105 58-59 0.8249501194454751 1105 60-61 0.7230187776640733 1105 62-63 0.7696797112252085 1105 64-65 0.7670085399905773 1105 66-67 0.7294634163756726 1105 68-69 0.6727577951899164 1105 70-71 0.6548070754346682 1105 72-73 0.6693475522383636 1105 74-75 0.7007908714826314 1105 76-77 0.6892284974945184 1105 78-79 0.7285122021176065 1105 80-81 0.7087079947075949 1105 82-83 0.7052573420940575 1105 84-85 0.6807553801976667 1105 86-87 0.6751189088766303 1105 88-89 0.7061287226983879 1105 90-91 0.7430597710506106 1105 92-93 0.6982265244386525 1105 94-95 0.7197586804147846 1105 96-97 0.7036642023565847 1105 98-99 0.659139881502302 1105 100 0.5528257362992051 1106 1 2.139318919013924 1106 2 1.3185736924343132 1106 3 0.7692756158791347 1106 4 0.45157510686050273 1106 5 0.4260982760223797 1106 6 0.3517949076758171 1106 7 0.3555054165058209 1106 8 0.36430292545311715 1106 9 0.401305828152843 1106 10-11 0.4014345822680596 1106 12-13 0.4199795297762208 1106 14-15 0.5249477498135917 1106 16-17 0.5191689437555596 1106 18-19 0.4609392149156051 1106 20-21 0.47018617934860885 1106 22-23 0.4793865199598457 1106 24-25 0.5340924587914131 1106 26-27 0.5568486417610714 1106 28-29 0.5818162368040731 1106 30-31 0.5955603422859355 1106 32-33 0.5904960171813087 1106 34-35 0.5590763249577506 1106 36-37 0.5574406775607272 1106 38-39 0.595139978043413 1106 40-41 0.6832979178891065 1106 42-43 0.6026696774893878 1106 44-45 0.5552628699515836 1106 46-47 0.6248077231867271 1106 48-49 0.5864458720508239 1106 50-51 0.6582066192097358 1106 52-53 0.6680704014368644 1106 54-55 0.7121516412265478 1106 56-57 0.6659989114647829 1106 58-59 0.6285950777111111 1106 60-61 0.5825341876312322 1106 62-63 0.6261560995020332 1106 64-65 0.6376793172704893 1106 66-67 0.5319815659950038 1106 68-69 0.4646955400819195 1106 70-71 0.48795250545011726 1106 72-73 0.49005420927556287 1106 74-75 0.5092484917064013 1106 76-77 0.4445986657623635 1106 78-79 0.5694047250920207 1106 80-81 0.5136342187571685 1106 82-83 0.5162522299909682 1106 84-85 0.5209661047064742 1106 86-87 0.4814309922078941 1106 88-89 0.5262558771564869 1106 90-91 0.5436798494722126 1106 92-93 0.5162449145489347 1106 94-95 0.5490223531147507 1106 96-97 0.5616459968415093 1106 98-99 0.547998681310041 1106 100 0.4063188409029763 1107 1 2.176622703396312 1107 2 1.406439657108237 1107 3 0.8555283086774175 1107 4 0.5360613684232618 1107 5 0.4395421994316351 1107 6 0.38277357113934585 1107 7 0.4068071115794112 1107 8 0.4604703695743311 1107 9 0.5357924032551082 1107 10-11 0.5251897042569027 1107 12-13 0.5263675240076822 1107 14-15 0.6829102950256214 1107 16-17 0.6409403871793842 1107 18-19 0.5898596574003392 1107 20-21 0.6050547591395556 1107 22-23 0.6698495007942853 1107 24-25 0.6922529837128408 1107 26-27 0.6994945054021926 1107 28-29 0.6977959660550468 1107 30-31 0.7257186634443116 1107 32-33 0.6780758693990308 1107 34-35 0.7445218130032245 1107 36-37 0.757031905979396 1107 38-39 0.763465672122372 1107 40-41 0.8539131668412097 1107 42-43 0.770995028896813 1107 44-45 0.8179998121296208 1107 46-47 0.8530779721237636 1107 48-49 0.8232756000007413 1107 50-51 0.7618476375050847 1107 52-53 0.8030018658969595 1107 54-55 0.8506239962504409 1107 56-57 0.8630718853416113 1107 58-59 0.7829626746914755 1107 60-61 0.7641857927979885 1107 62-63 0.8128887472256849 1107 64-65 0.7458274536780536 1107 66-67 0.7147106889420414 1107 68-69 0.7179937248955355 1107 70-71 0.6764088808217892 1107 72-73 0.688531539455532 1107 74-75 0.732302103358144 1107 76-77 0.6549110266600628 1107 78-79 0.782760652033069 1107 80-81 0.7282951870037166 1107 82-83 0.7096126788136417 1107 84-85 0.6747232975373691 1107 86-87 0.665167111233611 1107 88-89 0.7184560209304038 1107 90-91 0.7737221491615713 1107 92-93 0.7389218102075787 1107 94-95 0.750627999808259 1107 96-97 0.7237378455499552 1107 98-99 0.7560759079182766 1107 100 0.5518830276004962 1108 1 0.9853152720904035 1108 2 0.8123685453783267 1108 3 0.4112465401436616 1108 4 0.23381715572023865 1108 5 0.1807755691769941 1108 6 0.18494105629989122 1108 7 0.1702895209101669 1108 8 0.21277210330279672 1108 9 0.1654546887542594 1108 10-11 0.20702932795371254 1108 12-13 0.19984370082379854 1108 14-15 0.2714289875568596 1108 16-17 0.28127808315208824 1108 18-19 0.23315952629310743 1108 20-21 0.2233171419269695 1108 22-23 0.20227466797646798 1108 24-25 0.20252519657463353 1108 26-27 0.2550278143314202 1108 28-29 0.27312795664420975 1108 30-31 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-1.0772078248574743 2316 92-93 -1.0856509121257147 2316 94-95 -1.2543290451370765 2316 96-97 -1.1536852515306606 2316 98-99 -1.1538046566296032 2316 100 -1.1000494498937243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 404.0 8 816.0 9 442.0 10 191.0 11 178.0 12 293.0 13 1081.0 14 4411.0 15 11232.0 16 20495.0 17 31388.0 18 46272.0 19 68428.0 20 101927.0 21 152059.0 22 230599.0 23 352980.0 24 523725.0 25 646333.0 26 638545.0 27 571039.0 28 563548.0 29 630099.0 30 739403.0 31 888394.0 32 1079459.0 33 1337098.0 34 1734414.0 35 2419474.0 36 3709207.0 37 6189979.0 38 7243809.0 39 2014140.0 40 3370.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.51222477898346 35.600411603298355 28.319041865413663 18.568321752304524 2 20.89686503088085 34.21450433032226 26.818998059254312 18.06963257954258 3 26.643978266727625 26.378848065714912 26.741580403566324 20.235593263991138 4 19.84111652663696 27.31031649485501 26.033327300937753 26.81523967757028 5 20.202831816988528 33.62479828427529 26.173942723577476 19.998427175158707 6 27.432991199597513 26.45268258482653 26.169580372147383 19.944745843428574 7 20.098038371981712 26.547781220127163 33.40265633404822 19.951524073842904 8 27.120973544153614 26.642166357489444 26.228478375207477 20.00838172314947 9 27.015319520493087 26.441235324043692 26.448911685634812 20.09453346982841 10-11 19.72489772648576 30.42433756488724 29.996961687945856 19.853803020681145 12-13 19.769004729521225 26.737992572924576 29.955876098329153 23.537126599225047 14-15 19.706478213919386 33.99275042283358 26.317041306805272 19.983730056441757 16-17 23.424949389369377 26.658193461398493 26.31780174539746 23.599055403834672 18-19 19.83762654176582 30.25054231458887 26.309130471913438 23.602700671731867 20-21 23.471959871514535 30.252555006227354 26.272442486931563 20.003042635326544 22-23 23.475002480422578 26.714759039288094 29.86721849269136 19.943019987597964 24-25 19.936546875891384 30.26581584749645 26.108314995445348 23.68932228116682 26-27 19.97894054506173 26.62639273311112 33.35205587389838 20.042610847928774 28-29 19.967763975382695 26.70942918506362 33.30059483509482 20.02221200445886 30-31 20.031877085084286 26.66984256699284 33.28620908938167 20.0120712585412 32-33 20.000863395092942 26.726800356360926 33.219985214945794 20.05235103360034 34-35 20.058260861918654 26.740996774770267 33.168413083261484 20.0323292800496 36-37 20.100665483908227 26.714129509201356 33.11992651722285 20.065278489667566 38-39 20.118232241263197 26.732509904556196 33.073932499903066 20.075325354277542 40-41 20.064899022632492 26.83486662022043 33.07688282649629 20.02335153065079 42-43 20.10616705165928 26.845875721573538 33.03763788664147 20.010319340125722 44-45 20.130695757184252 26.862524081567248 32.99378225709404 20.01299790415446 46-47 20.169128132298084 26.933433501591157 32.92831346030211 19.96912490580865 48-49 20.205174471763122 26.963478684287878 32.81324183348006 20.01810501046894 50-51 20.230237335388125 27.011999251578217 32.69950880986885 20.05825460316481 52-53 20.357595180671538 27.174472368624482 32.400264676462065 20.06766777424191 54-55 20.726129355361184 27.458073155632086 31.522373339486105 20.29342414952063 56-57 21.12160445170175 28.02275263739605 30.33197794271021 20.523664968191994 58-59 21.18913005918935 28.55348651236982 29.60319375446464 20.65418967397619 60-61 21.20815041212486 28.86420391170238 29.19726506226224 20.730380613910523 62-63 21.14442197447361 29.029316349077135 29.113656191796522 20.712605484652737 64-65 21.151439843533655 29.171439879208556 29.00380385153072 20.67331642572707 66-67 21.10447102530248 29.052337358223184 29.145203183089546 20.697988433384783 68-69 21.014667295754876 28.894524444705365 29.37092826989299 20.71987998964677 70-71 20.96210570024952 28.790671164228254 29.559599792102965 20.687623343419265 72-73 20.95494214113137 28.709866075530993 29.586057999581193 20.74913378375644 74-75 20.98883440754368 28.740953520256507 29.448945184268023 20.821266887931795 76-77 21.04688398204845 28.823620007259223 29.26458379749181 20.86491221320052 78-79 21.149382623365405 28.95422202801935 28.85346668448749 21.042928664127754 80-81 21.217162405213447 29.109045665092943 28.50062233967548 21.17316959001813 82-83 21.255748688473417 29.270652730687363 28.167463763788103 21.306134817051113 84-85 21.315402731611584 29.30856609825217 27.908546783713025 21.46748438642322 86-87 21.385712376118793 29.28750050520011 27.708749192755555 21.618037925925545 88-89 21.481590592947644 29.283066160712835 27.51121695340581 21.72412629293371 90-91 21.534145776494533 29.224278424333516 27.34361685435375 21.897958944818203 92-93 21.57184459341346 29.17906820463575 27.249865608062727 21.999221593888063 94-95 21.632245940454546 29.138028191228344 27.15654272209362 22.0731831462235 96-97 21.67181146776697 29.060342411490875 27.046199877854132 22.22164624288802 98-99 21.6954726560593 28.992641563991544 26.963919129179764 22.34796665076939 100 21.700771007209134 29.016315352772494 26.876502098140236 22.40641154187814 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 75038.0 1 48030.5 2 15676.0 3 9552.0 4 8810.0 5 8846.0 6 9109.5 7 9399.5 8 9515.0 9 9822.5 10 10737.0 11 14489.5 12 147242.5 13 181256.5 14 82540.0 15 85777.0 16 98889.0 17 112411.0 18 125519.0 19 138808.5 20 155306.5 21 179828.5 22 213128.5 23 248825.0 24 281939.5 25 317513.5 26 357368.0 27 403494.0 28 467080.5 29 530067.0 30 577012.0 31 628623.5 32 685057.5 33 730196.5 34 764819.5 35 813275.0 36 869770.0 37 905482.0 38 935982.0 39 962366.5 40 974013.0 41 994554.5 42 1031746.5 43 1040994.5 44 1027732.5 45 1028144.0 46 1029966.5 47 1025174.0 48 1011486.0 49 995570.5 50 981567.0 51 965272.0 52 943288.0 53 905438.5 54 852902.5 55 792800.0 56 727248.0 57 657328.5 58 581434.5 59 496337.0 60 408187.5 61 328365.5 62 256367.5 63 191544.5 64 138895.0 65 99064.0 66 70351.0 67 51533.0 68 37598.5 69 27625.5 70 20916.0 71 16196.0 72 13282.5 73 10517.5 74 8656.5 75 8051.5 76 6120.0 77 3140.0 78 1505.0 79 983.0 80 671.5 81 454.5 82 329.5 83 228.0 84 164.0 85 124.5 86 95.5 87 59.5 88 36.5 89 24.5 90 17.0 91 16.5 92 14.5 93 9.0 94 2.5 95 4.5 96 4.5 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10707476078338693 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.566089076524938E-4 20-21 0.008383600775622328 22-23 7.82344230647847E-6 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.004534467160834921 42-43 0.03548713430218634 44-45 0.012323486321164886 46-47 0.003155976626433415 48-49 1.5646884612956941E-6 50-51 0.0 52-53 7.82344230647847E-6 54-55 0.0 56-57 7.82344230647847E-6 58-59 0.0 60-61 0.0 62-63 6.2587538451827765E-6 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.694065383887082E-6 72-73 9.231661921644594E-5 74-75 2.0184481150714454E-4 76-77 3.598783460980097E-5 78-79 3.5674896917541824E-4 80-81 7.82344230647847E-5 82-83 6.1022849990532074E-5 84-85 1.283044538262469E-4 86-87 2.613029730363809E-4 88-89 4.803593576177781E-4 90-91 3.2858457687209576E-4 92-93 6.29004761440869E-4 94-95 3.943014922465149E-4 96-97 2.1905638458139718E-5 98-99 2.597382845750852E-4 100 0.11049204038285673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3.1955243E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.48878395451772 #Duplication Level Percentage of deduplicated Percentage of total 1 76.39408081340963 33.22285675898339 2 11.476068414883168 9.981605198842376 3 4.210489417910026 5.4932719391481655 4 2.085460839599733 3.6277662359583975 5 1.2492319961663956 2.7163790195175643 6 0.9058254495674665 2.363594836604606 7 0.6232756332162099 1.8973849549938488 8 0.4587048866310102 1.595881417086204 9 0.3389507403708206 1.32664999672894 >10 1.9583642580159062 15.738761366954746 >50 0.1758176081774432 5.308871741597086 >100 0.12295574198322202 9.31337076157899 >500 2.5504525426699116E-4 0.07300224861450895 >1k 4.482563760220549E-4 0.47173102198248673 >5k 2.1269531725532883E-5 0.05731522010375713 >10k+ 4.9628906954236735E-5 6.811557281304956 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2003416 6.269443796750348 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83350 0.2608335664979922 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41862 0.1310019767335207 No Hit TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 35015 0.10957513294453747 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.058125046960212444 3.1293769225913882E-6 0.0 0.0 6.2587538451827765E-6 2 0.05813443509098022 1.877626153554833E-5 0.0 3.1293769225913882E-6 9.388130767774165E-6 3 0.05813756446790281 1.877626153554833E-5 0.0 6.2587538451827765E-6 1.2517507690365553E-5 4 0.05815321135251577 2.5035015380731106E-5 0.0 6.2587538451827765E-6 2.1905638458139718E-5 5 0.05816259948328354 2.8164392303322494E-5 0.0 9.388130767774165E-6 2.1905638458139718E-5 6 0.058209540137122415 3.129376922591388E-5 0.0 9.388130767774165E-6 2.1905638458139718E-5 7 0.058212669514045 3.129376922591388E-5 0.0 9.388130767774165E-6 2.8164392303322494E-5 8 0.058218928267890185 3.442314614850527E-5 0.0 1.2517507690365553E-5 3.129376922591388E-5 9 0.05823144577558055 3.755252307109666E-5 0.0 1.564688461295694E-5 3.442314614850527E-5 10-11 0.05824709266019351 4.5375965377575126E-5 0.0 1.7211573074252635E-5 3.598783460980097E-5 12-13 0.05827212767557424 5.31994076840536E-5 0.0 1.877626153554833E-5 4.5375965377575126E-5 14-15 0.058306550821722744 5.31994076840536E-5 0.0 2.0340949996844023E-5 5.31994076840536E-5 16-17 0.05833315052556477 5.31994076840536E-5 0.0 2.347032691943541E-5 5.789347306794068E-5 18-19 0.058358185540945504 5.31994076840536E-5 0.0 3.129376922591388E-5 6.1022849990532074E-5 20-21 0.05838791462171013 6.415222691312346E-5 0.0 3.129376922591388E-5 6.415222691312346E-5 22-23 0.05844267871785547 6.728160383571484E-5 0.0 3.285845768720957E-5 6.884629229701053E-5 24-25 0.05863200602167225 7.197566921960192E-5 0.0 3.598783460980097E-5 8.13637999873761E-5 26-27 0.05929543392926162 7.666973460348901E-5 0.0 3.755252307109666E-5 8.605786537126318E-5 28-29 0.06150007997122726 7.979911152608039E-5 0.0 3.755252307109666E-5 9.857537306162873E-5 30-31 0.06745371956645738 8.762255383255887E-5 0.0 3.755252307109666E-5 1.1109288075199429E-4 32-33 0.08012143734910732 9.388130767774163E-5 0.0 4.068189999368805E-5 1.2048101151976844E-4 34-35 0.10244015356102909 1.0014006152292442E-4 0.0 4.3811276916279435E-5 1.283044538262469E-4 36-37 0.13879256058231196 1.048341269068115E-4 0.0 5.16347192227579E-5 1.3925727305531677E-4 38-39 0.1927414540393262 1.0952819229069858E-4 0.0 5.47640961453493E-5 1.4395133843920384E-4 40-41 0.2658718633433643 1.1735163459717705E-4 0.0 5.632878460664499E-5 1.5177478074568233E-4 42-43 0.36039312860177597 1.283044538262469E-4 0.0 5.9458161529236376E-5 1.6742166535863926E-4 44-45 0.47793409050276975 1.4082196151661246E-4 0.0 6.1022849990532074E-5 1.815038615103005E-4 46-47 0.6226834200572344 1.5021009228438663E-4 0.0 6.571691537441915E-5 1.9871543458455316E-4 48-49 0.7958365392495999 1.5177478074568233E-4 0.0 6.571691537441915E-5 2.127976307362144E-4 50-51 0.9976563157413636 1.6429228843604788E-4 0.0 6.571691537441915E-5 2.2375044996528426E-4 52-53 1.2295415810169241 1.7837448458770912E-4 0.0 6.571691537441915E-5 2.2687982688787564E-4 54-55 1.4909994582109733 1.9402136920066608E-4 0.0 6.884629229701054E-5 2.331385807330584E-4 56-57 1.783532048246355 2.0028012304584885E-4 0.0 7.197566921960192E-5 2.409620230395369E-4 58-59 2.1091812695650605 2.065388768910316E-4 0.0 7.354035768089763E-5 2.5191484226860675E-4 60-61 2.4648208746214197 2.2687982688787564E-4 0.0 7.510504614219332E-5 2.581735961137895E-4 62-63 2.8480412431850386 2.378326461169455E-4 0.0 7.666973460348901E-5 2.65997038420268E-4 64-65 3.25977649426731 2.4252671150083258E-4 0.0 8.292848844867178E-5 2.691264153428594E-4 66-67 3.7022782145640387 2.4878546534601537E-4 0.0 8.449317690996748E-5 2.738204807267465E-4 68-69 4.1734559802909335 2.5504421919119813E-4 0.0 9.075193075515025E-5 2.8946736533970343E-4 70-71 4.673109511324949 2.6599703842026797E-4 0.0 9.075193075515025E-5 3.0042018456877327E-4 72-73 5.197142453274413 2.816439230332249E-4 0.0 9.075193075515025E-5 3.0667893841395603E-4 74-75 5.746873838512197 2.8946736533970343E-4 0.0 9.231661921644594E-5 3.129376922591388E-4 76-77 6.323082881892026 3.0354956149136465E-4 0.0 9.701068460033304E-5 3.191964461043216E-4 78-79 6.922815451598975 3.129376922591388E-4 0.0 1.0170474998422011E-4 3.3014926533339145E-4 80-81 7.548952139090289 3.145023807204345E-4 0.0 1.048341269068115E-4 3.3953739610116565E-4 82-83 8.199034818793272 3.238905114882087E-4 0.0 1.0796350382940289E-4 3.4266677302375703E-4 84-85 8.871275051796665 3.348433307172785E-4 0.0 1.1265756921328998E-4 3.5674896917541824E-4 86-87 9.562150724374089 3.3953739610116565E-4 0.0 1.1265756921328998E-4 3.708311653270795E-4 88 10.093382797933973 3.4110208456246134E-4 0.0 1.1265756921328998E-4 3.7865460763355797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 243060 0.0 88.7571 8 GTGGTAT 246815 0.0 87.73046 6 TGGTATC 246655 0.0 87.4768 7 GTATCAA 246845 0.0 87.354256 9 AGTGGTA 248445 0.0 87.31378 5 GCAGTGG 257655 0.0 84.4499 3 AAGCAGT 252575 0.0 83.98761 1 CAGTGGT 260455 0.0 83.25693 4 AGCAGTG 265520 0.0 81.76077 2 CAACGCA 239220 0.0 45.732426 12-13 ATCAACG 240535 0.0 45.47648 10-11 TCAACGC 238450 0.0 45.228718 12-13 ACGCAGA 243140 0.0 45.14224 14-15 AACGCAG 241270 0.0 44.866753 14-15 AGAGTAC 244995 0.0 44.48004 18-19 CGCAGAG 244655 0.0 44.274803 16-17 GAGTACT 241565 0.0 44.129356 20-21 TATCAAC 245775 0.0 43.84529 10-11 AGTACTT 253275 0.0 42.681957 20-21 GTACTTT 252610 0.0 42.16407 22-23 >>END_MODULE