FastQCFastQC Report
Thu 26 May 2016
SRR1660768_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660768_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23799380
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10670644.4835789839903395No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751720.31585696770252No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC584870.24575009937233658Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1339300.087.2095958
GTGGTAT1363200.085.432536
TGGTATC1363050.085.317887
GTATCAA1370150.085.2562949
AGTGGTA1371450.084.774775
GCAGTGG1451400.080.988453
AAGCAGT1454500.080.98261
CAGTGGT1459650.079.691164
AGCAGTG1500450.078.62682
CAACGCA1325800.045.2939912-13
ATCAACG1340050.044.93676810-11
TCAACGC1324400.044.77443312-13
ACGCAGA1343950.044.60054414-15
AACGCAG1336000.044.36997614-15
AGAGTAC1343800.043.77518-19
CGCAGAG1356200.043.61101516-17
GAGTACT1329100.043.323120-21
TATCAAC1372350.042.78389710-11
AGTACTT1420200.041.1815620-21
GTACTTT1406100.041.0387422-23
Produced by FastQC (version 0.11.5)