FastQCFastQC Report
Thu 26 May 2016
SRR1660768_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660768_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23799380
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12017855.049648352183964No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA539980.22688826347577123No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG449050.18868138581761373TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263880.11087683796804791No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1491000.087.5756158
GTGGTAT1522350.086.031476
TGGTATC1520700.085.855957
GTATCAA1521650.085.771469
AGTGGTA1529600.085.7066655
GCAGTGG1598450.082.2384643
AAGCAGT1568100.081.703661
CAGTGGT1615950.081.0453954
AGCAGTG1644300.079.8624342
CAACGCA1458750.045.5081812-13
ATCAACG1472250.045.11797310-11
ACGCAGA1486200.044.815114-15
TCAACGC1454550.044.80114412-13
AACGCAG1474950.044.35066214-15
AGAGTAC1505650.043.99745618-19
CGCAGAG1494850.043.81846616-17
GAGTACT1487200.043.4732620-21
TATCAAC1517100.042.9926710-11
AGTACTT1577900.041.6802920-21
GTACTTT1557350.041.38820622-23
Produced by FastQC (version 0.11.5)