##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660768_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23799380 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.185011584335392 31.0 31.0 34.0 25.0 34.0 2 31.246968660528132 33.0 31.0 34.0 27.0 34.0 3 31.887222860427457 33.0 31.0 34.0 30.0 34.0 4 35.699610452036985 37.0 35.0 37.0 33.0 37.0 5 35.65502084508084 37.0 35.0 37.0 33.0 37.0 6 35.69592287698251 37.0 35.0 37.0 33.0 37.0 7 35.63112723944909 37.0 35.0 37.0 33.0 37.0 8 35.62724222227638 37.0 35.0 37.0 33.0 37.0 9 37.294852554982526 39.0 37.0 39.0 34.0 39.0 10-11 37.19806795387107 39.0 37.0 39.0 33.0 39.0 12-13 37.25808794178672 39.0 37.0 39.0 33.0 39.0 14-15 38.52095600809769 40.0 38.0 41.0 34.0 41.0 16-17 38.51030919713035 40.0 38.0 41.0 34.0 41.0 18-19 38.48304464233942 40.0 38.0 41.0 33.5 41.0 20-21 38.413138934711746 40.0 38.0 41.0 34.0 41.0 22-23 38.29005200975824 40.0 37.5 41.0 33.5 41.0 24-25 38.28418656284323 40.0 38.0 41.0 33.5 41.0 26-27 38.20917729789599 40.0 38.0 41.0 33.0 41.0 28-29 38.09449515071401 40.0 37.5 41.0 33.0 41.0 30-31 38.02392560226359 40.0 37.0 41.0 33.0 41.0 32-33 37.91753257857978 40.0 37.0 41.0 33.0 41.0 34-35 37.81224405845867 40.0 37.0 41.0 33.0 41.0 36-37 37.62414369618032 39.5 37.0 41.0 32.0 41.0 38-39 37.241691863401485 39.0 35.0 41.0 31.0 41.0 40-41 37.03449278930796 39.0 35.0 41.0 31.0 41.0 42-43 36.9100891073633 39.0 35.0 40.5 31.0 41.0 44-45 36.813715504353475 39.0 35.0 40.0 31.0 41.0 46-47 36.91431005765696 39.0 35.0 41.0 31.0 41.0 48-49 36.87703809931183 39.0 35.0 41.0 31.0 41.0 50-51 36.72626314214908 39.0 35.0 41.0 30.5 41.0 52-53 36.50555218245181 39.0 35.0 41.0 30.0 41.0 54-55 36.15333206159152 39.0 35.0 41.0 28.5 41.0 56-57 35.746961391431206 39.0 35.0 41.0 27.0 41.0 58-59 35.52969634082905 38.5 35.0 40.5 26.0 41.0 60-61 35.34833033465577 38.0 34.0 40.0 26.0 41.0 62-63 35.101591428011986 38.0 34.0 40.0 26.0 41.0 64-65 34.82963247361906 37.0 34.0 40.0 26.0 41.0 66-67 34.6001493526302 37.0 34.0 40.0 26.0 41.0 68-69 34.37029525559069 36.0 34.0 39.0 26.0 41.0 70-71 34.04855670609906 36.0 33.0 39.0 25.5 41.0 72-73 33.711047997889025 35.0 33.0 39.0 25.0 40.5 74-75 33.34845172437265 35.0 33.0 37.5 24.5 40.0 76-77 31.289169234660736 33.5 30.0 35.5 21.0 38.5 78-79 32.55598049192878 35.0 32.0 37.0 24.0 39.0 80-81 32.56529974730434 35.0 32.5 36.0 24.0 39.0 82-83 32.366975652306905 35.0 32.5 36.0 24.0 37.5 84-85 32.1288974964894 35.0 32.0 35.5 23.5 37.0 86-87 31.921210195391645 35.0 32.0 35.0 23.0 37.0 88-89 31.72683170738061 35.0 32.0 35.0 23.0 36.0 90-91 31.570146386166364 35.0 32.0 35.0 22.5 36.0 92-93 31.43524240967622 35.0 32.0 35.0 20.5 36.0 94-95 31.30567689158289 35.0 32.0 35.0 20.0 35.5 96-97 31.200823992053575 35.0 32.0 35.0 20.0 35.0 98-99 31.07792177779421 35.0 31.5 35.0 19.5 35.0 100 30.935671265385906 34.0 31.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.3757411667269928 1101 2 1.5395215330523726 1101 3 0.9940641406871116 1101 4 0.6260857613327389 1101 5 0.6231762017478744 1101 6 0.5323584795081189 1101 7 0.5350046200110015 1101 8 0.5794182473817884 1101 9 0.6793340878812302 1101 10-11 0.7263488483203133 1101 12-13 0.7003942854363814 1101 14-15 0.9212123307488724 1101 16-17 0.9182461049165198 1101 18-19 0.8824176615273132 1101 20-21 0.8846060223452525 1101 22-23 0.8993523150689171 1101 24-25 0.8708698599007008 1101 26-27 0.9003013324241067 1101 28-29 0.9199901574803064 1101 30-31 0.8617398827721701 1101 32-33 0.863461135252173 1101 34-35 0.8960568846280808 1101 36-37 0.9164617080197033 1101 38-39 0.99251644640038 1101 40-41 1.0319737477068713 1101 42-43 0.9858419349956975 1101 44-45 0.9783716761057022 1101 46-47 0.8841409896900672 1101 48-49 0.820094272362681 1101 50-51 0.8149632545175223 1101 52-53 0.9369193900812647 1101 54-55 1.0458466062056786 1101 56-57 1.0608262346401531 1101 58-59 0.9747021750660352 1101 60-61 0.9988369245111173 1101 62-63 1.0250764648679294 1101 64-65 1.0107459563320447 1101 66-67 0.9553470499452956 1101 68-69 0.931083102771467 1101 70-71 0.9202485870928925 1101 72-73 0.9098547829622134 1101 74-75 0.8452665732968896 1101 76-77 1.0877722426874037 1101 78-79 0.9441899834406158 1101 80-81 0.8312366945998662 1101 82-83 0.8010452978462439 1101 84-85 0.7779923820058627 1101 86-87 0.7635475715420341 1101 88-89 0.794383809197786 1101 90-91 0.7716179454776864 1101 92-93 0.7469287948143268 1101 94-95 0.7387024100539641 1101 96-97 0.6855118706812782 1101 98-99 0.6535979971929535 1101 100 0.527528189623002 1102 1 2.3579764053120265 1102 2 1.5646358478225082 1102 3 0.9751999773318438 1102 4 0.6024338791366617 1102 5 0.5879002815409393 1102 6 0.5419678341206975 1102 7 0.4761426270738198 1102 8 0.5504211188161534 1102 9 0.6253176805382239 1102 10-11 0.7206197853048479 1102 12-13 0.607356515404895 1102 14-15 0.8658066345850557 1102 16-17 0.8439874127626652 1102 18-19 0.8254448892120223 1102 20-21 0.7862646056242042 1102 22-23 0.8138303754275569 1102 24-25 0.7992075608040423 1102 26-27 0.8706544224987738 1102 28-29 0.8822635620156021 1102 30-31 0.8670926791434823 1102 32-33 0.8340650070700661 1102 34-35 0.8393676881661918 1102 36-37 0.8848162324287827 1102 38-39 0.9949321206243908 1102 40-41 1.02815197553727 1102 42-43 0.9662001823023445 1102 44-45 0.9552446379509263 1102 46-47 0.8843847081715737 1102 48-49 0.8402467038559536 1102 50-51 0.849536261264511 1102 52-53 0.9345076540266675 1102 54-55 1.0599293230292872 1102 56-57 1.0668688135526025 1102 58-59 0.9677389992731236 1102 60-61 0.8957202506005046 1102 62-63 0.9093821794283059 1102 64-65 0.878300927258735 1102 66-67 0.9154776976093331 1102 68-69 0.8759452685013613 1102 70-71 0.8403340973618327 1102 72-73 0.8871610817212598 1102 74-75 0.8411893345111849 1102 76-77 0.7425392916699316 1102 78-79 0.8975088148706973 1102 80-81 0.8204614150645142 1102 82-83 0.8325512228553151 1102 84-85 0.7669989765590444 1102 86-87 0.8009512094592139 1102 88-89 0.8025923425692874 1102 90-91 0.8262383363675454 1102 92-93 0.7564797793400864 1102 94-95 0.732238258955693 1102 96-97 0.7743680791555754 1102 98-99 0.7469620877031495 1102 100 0.6039075583499347 1103 1 1.998440194531664 1103 2 1.2554635711072422 1103 3 0.7285768117327827 1103 4 0.5117137730360568 1103 5 0.4623147437906425 1103 6 0.46009973104054325 1103 7 0.4971604020713798 1103 8 0.5040677661868358 1103 9 0.6207842836400559 1103 10-11 0.5844530000957917 1103 12-13 0.6012419439477767 1103 14-15 0.7824356300759874 1103 16-17 0.7666141433378826 1103 18-19 0.7600539579475765 1103 20-21 0.6949607403276445 1103 22-23 0.7185019555354017 1103 24-25 0.7380525684564319 1103 26-27 0.7133397997523332 1103 28-29 0.7863411154379278 1103 30-31 0.7734585101100109 1103 32-33 0.7740698392975744 1103 34-35 0.7866208637435648 1103 36-37 0.7903131035959916 1103 38-39 0.8861244161613726 1103 40-41 0.8873170187674475 1103 42-43 0.8046465698952829 1103 44-45 0.8068994090882242 1103 46-47 0.7548234068021102 1103 48-49 0.7039733037304359 1103 50-51 0.6789079248012015 1103 52-53 0.7727506754397879 1103 54-55 0.8925313664333814 1103 56-57 0.8336677593260262 1103 58-59 0.8366675002171391 1103 60-61 0.7655175293035725 1103 62-63 0.7721258371872466 1103 64-65 0.7603978444926014 1103 66-67 0.7737827051981085 1103 68-69 0.6996538371827725 1103 70-71 0.6873257082767523 1103 72-73 0.6676198709015608 1103 74-75 0.6950548612403082 1103 76-77 0.6581381987085386 1103 78-79 0.7509012670711073 1103 80-81 0.7027436053495677 1103 82-83 0.6670942277132355 1103 84-85 0.6492033142898563 1103 86-87 0.6345366615878163 1103 88-89 0.578618457521987 1103 90-91 0.608564864965949 1103 92-93 0.6430863863890544 1103 94-95 0.6555442863884515 1103 96-97 0.6445609313591341 1103 98-99 0.6594089926252344 1103 100 0.44700216421643546 1104 1 2.1471987960891035 1104 2 1.4086391647260825 1104 3 0.854587651408071 1104 4 0.5288694820096609 1104 5 0.45351537188714985 1104 6 0.4271735414762361 1104 7 0.44715450249374555 1104 8 0.4712252864204629 1104 9 0.5177795621839962 1104 10-11 0.5467850427083363 1104 12-13 0.5441387946402401 1104 14-15 0.7038918750377832 1104 16-17 0.7060547389028429 1104 18-19 0.6252057980319847 1104 20-21 0.6814141282667094 1104 22-23 0.6819219461999424 1104 24-25 0.6995915789532674 1104 26-27 0.6697275635058588 1104 28-29 0.7216611357372571 1104 30-31 0.7183910239145348 1104 32-33 0.7321014120148135 1104 34-35 0.6221119281258893 1104 36-37 0.6904217115314069 1104 38-39 0.7596786636039141 1104 40-41 0.8706119472771263 1104 42-43 0.8214395685396525 1104 44-45 0.7280634074011587 1104 46-47 0.7884712796290643 1104 48-49 0.7839765472392344 1104 50-51 0.7815139271029352 1104 52-53 0.849827624579838 1104 54-55 0.9984082844373674 1104 56-57 0.9802487593467717 1104 58-59 0.9255250101056589 1104 60-61 0.8171648518127554 1104 62-63 0.8575797223494561 1104 64-65 0.8835777169968893 1104 66-67 0.8621024864457425 1104 68-69 0.8164687751273689 1104 70-71 0.8016779462970263 1104 72-73 0.7428279077282696 1104 74-75 0.7170643104155161 1104 76-77 0.5815566630206277 1104 78-79 0.788281873798212 1104 80-81 0.7337039424214424 1104 82-83 0.7382901957099222 1104 84-85 0.7274572382171272 1104 86-87 0.7509087397665155 1104 88-89 0.7632212715654454 1104 90-91 0.7619018806439044 1104 92-93 0.7479145793265438 1104 94-95 0.7267056224953343 1104 96-97 0.7223818838830063 1104 98-99 0.7123824120144526 1104 100 0.5912464833984075 1105 1 2.2022002972721815 1105 2 1.3654816935744165 1105 3 0.8299243041225104 1105 4 0.49998266616714915 1105 5 0.4131540688159987 1105 6 0.3708861879942589 1105 7 0.3786372890588581 1105 8 0.4042442675141871 1105 9 0.49044665594567505 1105 10-11 0.45107484178127777 1105 12-13 0.4500536918120801 1105 14-15 0.5772113009976607 1105 16-17 0.583815237772825 1105 18-19 0.5121007367367056 1105 20-21 0.4146420591248585 1105 22-23 0.5089340759164216 1105 24-25 0.5532940535184068 1105 26-27 0.6099691348625029 1105 28-29 0.5976306512534393 1105 30-31 0.6260490596576673 1105 32-33 0.6335136723207881 1105 34-35 0.5611892657758304 1105 36-37 0.6277001919527336 1105 38-39 0.7021282082292046 1105 40-41 0.7226786734889217 1105 42-43 0.6657520584139078 1105 44-45 0.6695942053132811 1105 46-47 0.66686624112441 1105 48-49 0.6164515023660044 1105 50-51 0.686579187199797 1105 52-53 0.7057765111473842 1105 54-55 0.7551115390399374 1105 56-57 0.661161530581559 1105 58-59 0.6495996625202096 1105 60-61 0.5512689320230422 1105 62-63 0.5999626750018621 1105 64-65 0.6169051733400366 1105 66-67 0.6481551984585252 1105 68-69 0.5742607828436732 1105 70-71 0.5341654019885382 1105 72-73 0.5553084509018476 1105 74-75 0.5827811331496946 1105 76-77 0.5990363149553204 1105 78-79 0.6142518171529403 1105 80-81 0.5863500918406928 1105 82-83 0.5580098905206228 1105 84-85 0.5314301469683613 1105 86-87 0.5765184704898729 1105 88-89 0.56163759518574 1105 90-91 0.6127192597894364 1105 92-93 0.5630194347839499 1105 94-95 0.4842681341409083 1105 96-97 0.4872077008457403 1105 98-99 0.5064136391821101 1105 100 0.4288969173281707 1106 1 2.112500218589357 1106 2 1.3067920391737644 1106 3 0.7571895166721241 1106 4 0.4344906494006153 1106 5 0.39632389985037264 1106 6 0.3219408862651676 1106 7 0.3510860964309259 1106 8 0.3416610637717312 1106 9 0.3880561302475414 1106 10-11 0.4018755927131821 1106 12-13 0.37987783072222925 1106 14-15 0.5029305419525087 1106 16-17 0.4921998580575746 1106 18-19 0.4661403715650039 1106 20-21 0.48774694668836815 1106 22-23 0.5107355897320716 1106 24-25 0.5226466530700691 1106 26-27 0.5621497186210433 1106 28-29 0.5802131748981552 1106 30-31 0.5733246458220052 1106 32-33 0.5919023945193587 1106 34-35 0.5363295582384211 1106 36-37 0.5709364513040427 1106 38-39 0.6354341909386889 1106 40-41 0.7215126202598157 1106 42-43 0.6242212520469863 1106 44-45 0.62516410593107 1106 46-47 0.6637856006086338 1106 48-49 0.660270560612986 1106 50-51 0.6424432071465418 1106 52-53 0.7207114412840383 1106 54-55 0.7912955748807846 1106 56-57 0.7904213982461243 1106 58-59 0.7480024337842295 1106 60-61 0.6753301670919214 1106 62-63 0.7229637979533408 1106 64-65 0.7040556918758938 1106 66-67 0.6433521534967994 1106 68-69 0.6130474500764649 1106 70-71 0.5675540398546559 1106 72-73 0.5856155849597116 1106 74-75 0.6181397262793311 1106 76-77 0.5364015116436356 1106 78-79 0.6937782488871918 1106 80-81 0.6576247747087933 1106 82-83 0.6452122601361197 1106 84-85 0.5963463315075614 1106 86-87 0.5847840838672056 1106 88-89 0.6107615432275288 1106 90-91 0.6550142354943347 1106 92-93 0.5506201297581832 1106 94-95 0.5837264410525513 1106 96-97 0.6240555117369659 1106 98-99 0.627233324004397 1106 100 0.45833069254348047 1107 1 2.181336189884764 1107 2 1.4298814238769992 1107 3 0.8628460824950928 1107 4 0.529112608705141 1107 5 0.4157669500008794 1107 6 0.3824874981120914 1107 7 0.4031583762024127 1107 8 0.4483297137901374 1107 9 0.5328198216010378 1107 10-11 0.517194379396841 1107 12-13 0.5365262547850236 1107 14-15 0.6757766570645458 1107 16-17 0.6244287994082782 1107 18-19 0.610444558661257 1107 20-21 0.6112727393671165 1107 22-23 0.6521054697259459 1107 24-25 0.6617503592041132 1107 26-27 0.6927118391050016 1107 28-29 0.736409735178448 1107 30-31 0.7047510941227131 1107 32-33 0.6658304963597601 1107 34-35 0.7230787074039213 1107 36-37 0.7894051831103184 1107 38-39 0.7896575950683484 1107 40-41 0.8679110456815309 1107 42-43 0.8059589456662337 1107 44-45 0.8227104281819848 1107 46-47 0.8027923770954999 1107 48-49 0.8148996306360914 1107 50-51 0.7544418986726384 1107 52-53 0.8138213241880479 1107 54-55 0.8724415084062187 1107 56-57 0.8182722565059422 1107 58-59 0.7510829182219965 1107 60-61 0.7078843641976604 1107 62-63 0.7691663966229498 1107 64-65 0.7031203727940678 1107 66-67 0.6427349641491773 1107 68-69 0.6457238779135253 1107 70-71 0.6408914055384685 1107 72-73 0.6569780468053068 1107 74-75 0.6898528875129415 1107 76-77 0.5740091869066752 1107 78-79 0.6993593201095152 1107 80-81 0.6566652258779726 1107 82-83 0.6445956006866567 1107 84-85 0.6253606979113755 1107 86-87 0.6078689217423872 1107 88-89 0.7034675727205943 1107 90-91 0.6965863301316588 1107 92-93 0.6817991244911958 1107 94-95 0.710201839535177 1107 96-97 0.6960000360961303 1107 98-99 0.7353442077147321 1107 100 0.5669237676084364 1108 1 1.0082161391720206 1108 2 0.8071734414289367 1108 3 0.39745996657231686 1108 4 0.238539654038199 1108 5 0.19085647171985443 1108 6 0.19997519317524137 1108 7 0.1771350421843394 1108 8 0.22967122094533465 1108 9 0.18208593217300972 1108 10-11 0.20719722439414312 1108 12-13 0.2084932778811961 1108 14-15 0.2712448784378978 1108 16-17 0.3035865395581965 1108 18-19 0.2567443355568031 1108 20-21 0.21576786404842352 1108 22-23 0.17729298787753578 1108 24-25 0.21013633577174318 1108 26-27 0.24361554292777754 1108 28-29 0.2727190779261761 1108 30-31 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2316 88-89 -0.9853878184578271 2316 90-91 -0.9652783667493274 2316 92-93 -0.9730488628488594 2316 94-95 -1.0948717362216058 2316 96-97 -1.0276232073081708 2316 98-99 -1.009835458246048 2316 100 -0.9598810014437333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 13.0 7 267.0 8 561.0 9 273.0 10 134.0 11 105.0 12 165.0 13 720.0 14 2710.0 15 7170.0 16 13451.0 17 20779.0 18 31047.0 19 47388.0 20 68449.0 21 101542.0 22 154077.0 23 233771.0 24 347055.0 25 428656.0 26 426347.0 27 395323.0 28 405441.0 29 461233.0 30 544650.0 31 654319.0 32 798987.0 33 993730.0 34 1292907.0 35 1807158.0 36 2797694.0 37 4737301.0 38 5501296.0 39 1522253.0 40 2408.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.52242062917033 34.58970487326927 28.20763301444244 19.680241483117957 2 21.623000263032065 32.40180206375124 27.52912470829072 18.44607296492598 3 25.080586132916068 27.074675894918272 26.764453527780972 21.080284444384688 4 19.783750669135078 27.690486054678736 26.740797449345322 25.784965826840867 5 20.675425158134374 32.38810422792526 26.417881474223275 20.518589139717086 6 26.57833523394307 26.622298564080243 26.520993404029852 20.278372797946837 7 20.737418369722235 26.832934303330592 32.11866443579623 20.310982891150946 8 25.840946276751747 26.929764556891815 26.70515366366687 20.524135502689568 9 25.79019705555355 26.624109535626555 27.023855243287848 20.56183816553204 10-11 20.260924864429242 29.874498831482164 29.553545092351147 20.311031211737447 12-13 20.369425590078396 26.833306161757154 29.395631314765343 23.401636933399107 14-15 20.16858842541276 32.79014621389297 26.483547050385347 20.55771831030892 16-17 23.164424871572283 26.885032299160734 26.486332837241978 23.464209992025005 18-19 20.417140025729726 29.638875224284224 26.587387098939473 23.356597651046577 20-21 23.216597531924695 29.61775249991391 26.55159638547445 20.614053582686946 22-23 23.37088438552647 26.909010540296695 29.297612931262595 20.42249214291424 24-25 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27.137920279348464 31.66414762334765 20.463454109453863 50-51 20.89652545570515 27.07126404133217 31.4260980748238 20.606112428138886 52-53 21.027940310036676 27.204502991632808 31.15679115647476 20.61076554185576 54-55 21.204207840708456 27.42955278666923 30.53313363625439 20.833105736367923 56-57 21.51829016364588 27.872076231239163 29.71041636930244 20.899217235812518 58-59 21.600411439289598 28.343675339441614 28.937463917127253 21.11844930414154 60-61 21.730482054574534 28.614050029874726 28.465619692613842 21.1898482229369 62-63 21.569342373318058 28.789298324685102 28.581238252186985 21.060121049809855 64-65 21.695642911706102 29.014344071148074 28.248664461006967 21.041348556138857 66-67 21.518842539571253 29.037063760422832 28.337837547403282 21.106256152602633 68-69 21.409606498354293 28.926348689048805 28.530893000802816 21.133151811794086 70-71 21.36462272446507 28.822488269808243 28.671080941973415 21.141808063753267 72-73 21.33095775104522 28.720270521582975 28.69572154005163 21.25305018732018 74-75 21.336456840337057 28.70060724851894 28.575689798156812 21.387246112987192 76-77 21.359354250038546 28.751460674383917 28.433370274792818 21.45581480078472 78-79 21.39198375783718 28.84485205411665 28.099029494299906 21.664134693746266 80-81 21.391050000392866 28.947789042836586 27.841521574136912 21.81963938263364 82-83 21.382146120285142 29.094406510425802 27.545650902870726 21.977796466418326 84-85 21.384519645068377 29.13527933510942 27.31612839168743 22.16407262813478 86-87 21.360938329527553 29.111747543994436 27.152815112535798 22.37449901394221 88-89 21.37286454690121 29.119324230876583 27.00201403384413 22.50579718837808 90-91 21.361399225581582 29.050880070778618 26.85371477009871 22.734005933541095 92-93 21.317021706656316 29.019225650688853 26.78601175193952 22.877740890715305 94-95 21.308161285954736 28.97835050279352 26.7092379780777 23.004250233174044 96-97 21.284907826876527 28.914369486002204 26.61416388184184 23.186558805279425 98-99 21.2268325595359 28.84147828748709 26.593558758778357 23.338130394198654 100 21.198838182651823 28.828688728016118 26.555850940769187 23.416622148562872 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 47085.0 1 29780.0 2 9398.5 3 6047.0 4 5623.5 5 5533.5 6 5710.5 7 5901.0 8 6005.0 9 6247.5 10 6779.0 11 8811.0 12 86887.0 13 105996.0 14 47241.5 15 49183.0 16 56742.0 17 65266.0 18 73307.0 19 81833.0 20 93483.0 21 109774.5 22 131436.5 23 154612.0 24 177466.0 25 205528.0 26 238580.5 27 276538.0 28 322532.5 29 365810.0 30 402207.0 31 438863.0 32 477950.0 33 515187.0 34 546268.0 35 581935.0 36 626995.0 37 663851.0 38 692899.5 39 714604.5 40 726857.0 41 741298.0 42 763841.0 43 779881.5 44 789046.5 45 802972.5 46 810635.5 47 811226.0 48 801848.0 49 786941.0 50 776766.5 51 766444.5 52 749611.0 53 718236.5 54 672970.0 55 624303.0 56 574753.0 57 520099.5 58 458897.0 59 391286.0 60 320072.0 61 255655.5 62 199097.5 63 148263.5 64 107848.5 65 76946.0 66 53638.5 67 38311.0 68 27767.0 69 20115.0 70 15011.0 71 11515.5 72 9070.5 73 6982.0 74 5443.0 75 4476.0 76 3318.5 77 1985.5 78 1189.5 79 801.5 80 503.5 81 343.0 82 237.5 83 152.5 84 114.5 85 91.5 86 55.5 87 37.0 88 25.5 89 14.0 90 10.0 91 5.5 92 4.0 93 2.5 94 0.5 95 0.5 96 2.5 97 2.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1076792756786101 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.4160293251336798E-4 20-21 0.008447699057706546 22-23 1.0504475326668174E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.004556841396708654 42-43 0.035666895524169115 44-45 0.012256621811156424 46-47 0.0032143694499604607 48-49 4.201790130667269E-6 50-51 0.0 52-53 8.403580261334539E-6 54-55 0.0 56-57 1.2605370392001807E-5 58-59 0.0 60-61 0.0 62-63 1.4706265457335444E-5 64-65 0.0 66-67 2.1008950653336347E-6 68-69 2.1008950653336347E-6 70-71 1.4706265457335444E-5 72-73 9.243938287467992E-5 74-75 2.64712778232038E-4 76-77 5.672416676400814E-5 78-79 3.6345484630271886E-4 80-81 1.1344833352801628E-4 82-83 6.302685196000904E-5 84-85 1.4916354963868806E-4 86-87 2.731163584933725E-4 88-89 5.357282416600768E-4 90-91 4.243808031973942E-4 92-93 6.701855258414295E-4 94-95 4.096745377400588E-4 96-97 3.151342598000452E-5 98-99 3.5715216110671793E-4 100 0.11042304463393583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.379938E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.788462440998714 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61880928913193 37.092835524287224 2 11.20454301396142 10.708957659824996 3 3.893890394438831 5.582491045920172 4 2.008089010201174 3.838539449687243 5 1.228717949048558 2.9359270779343993 6 0.7966607121439907 2.284271431230743 7 0.563042638022361 1.8834859371868704 8 0.4140161688589174 1.5828156908384448 9 0.30120858481123014 1.2954865627942063 >10 1.7189697923123217 15.23848385121743 >50 0.18364406763248303 6.060606078097852 >100 0.0677016546734771 5.428048251875176 >500 2.1905443194724698E-4 0.07415084745655308 >1k 4.2681331784138473E-4 0.3566521944505398 >5k 1.738771811533962E-5 0.06127294884096078 >10k+ 4.346929528833008E-5 5.5759754483572666 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1201785 5.049648352183964 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53998 0.22688826347577123 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCTCATCTCGTATG 44905 0.18868138581761373 TruSeq Adapter, Index 7 (97% over 35bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26388 0.11087683796804791 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.28971342950950824 0.0 0.0 0.0 8.403580261334539E-6 2 0.28972183308976956 0.0 0.0 0.0 1.6807160522669078E-5 3 0.28972603487990023 4.201790130667269E-6 0.0 0.0 1.6807160522669078E-5 4 0.2897386402502922 2.1008950653336348E-5 0.0 0.0 1.6807160522669078E-5 5 0.2897428420404229 2.9412530914670885E-5 0.0 0.0 1.6807160522669078E-5 6 0.2897428420404229 3.3614321045338155E-5 0.0 0.0 2.1008950653336348E-5 7 0.28974704383055355 4.2017901306672696E-5 0.0 8.403580261334539E-6 2.5210740784003618E-5 8 0.2897512456206842 4.6219691437339966E-5 0.0 1.2605370392001809E-5 2.5210740784003618E-5 9 0.2897638509910762 4.6219691437339966E-5 0.0 1.2605370392001809E-5 2.5210740784003618E-5 10-11 0.28976595188614157 5.252237663334087E-5 0.0 1.2605370392001809E-5 3.3614321045338155E-5 12-13 0.2897827590466643 5.4623271698674506E-5 0.0 1.2605370392001809E-5 3.7816111176005425E-5 14-15 0.2898100706825136 6.512774702534268E-5 0.0 1.2605370392001809E-5 4.2017901306672696E-5 16-17 0.28983948321342823 7.563222235201085E-5 0.0 1.2605370392001809E-5 4.2017901306672696E-5 18-19 0.2898962073801923 9.03384878093463E-5 0.0 1.6807160522669078E-5 4.6219691437339966E-5 20-21 0.2899424270716296 9.243938287467993E-5 0.0 2.5210740784003618E-5 4.6219691437339966E-5 22-23 0.29004116913970024 9.243938287467993E-5 0.0 2.5210740784003618E-5 4.83205865026736E-5 24-25 0.29036050518963097 9.243938287467993E-5 0.0 2.5210740784003618E-5 5.0421481568007236E-5 26-27 0.29148658494464985 1.0084296313601447E-4 0.0 2.5210740784003618E-5 5.0421481568007236E-5 28-29 0.29484381525905295 1.09246543397349E-4 0.0 2.9412530914670885E-5 5.46232716986745E-5 30-31 0.30340496265028755 1.1344833352801627E-4 0.0 2.9412530914670885E-5 6.0925956894675405E-5 32-33 0.32173947388545415 1.2185191378935081E-4 0.0 3.151342598000452E-5 7.563222235201085E-5 34-35 0.35490420338681095 1.2815459898535172E-4 0.0 3.3614321045338155E-5 7.983401248267812E-5 36-37 0.40627739041941424 1.3025549405068536E-4 0.0 3.3614321045338155E-5 7.983401248267812E-5 38-39 0.4802457038796809 1.3445728418135262E-4 0.0 3.571521611067179E-5 7.983401248267812E-5 40-41 0.5794205563338204 1.386590743120199E-4 0.0 3.991700624133906E-5 7.983401248267812E-5 42-43 0.7072621219544375 1.491635496386881E-4 0.0 4.2017901306672696E-5 7.983401248267812E-5 44-45 0.8677621013656658 1.575671299000226E-4 0.0 4.2017901306672696E-5 8.823759274401266E-5 46-47 1.0592880991017415 1.6807160522669078E-4 0.0 4.2017901306672696E-5 1.0084296313601447E-4 48-49 1.2810459768279678 1.7437429042269168E-4 0.0 5.0421481568007236E-5 1.0084296313601447E-4 50-51 1.5370085271128913 1.7857608055335897E-4 0.0 5.4623271698674506E-5 1.0084296313601447E-4 52-53 1.8274866824261808 1.8067697561869258E-4 0.0 5.4623271698674506E-5 1.0084296313601447E-4 54-55 2.1535582019363533 1.8487876574935984E-4 0.0 5.4623271698674506E-5 1.0084296313601447E-4 56-57 2.513244042491863 1.9118145094536076E-4 0.0 5.4623271698674506E-5 1.0714564833201538E-4 58-59 2.901138601089608 2.1429129666403077E-4 0.0 5.4623271698674506E-5 1.1344833352801628E-4 60-61 3.319328066529464 2.2689666705603257E-4 0.0 5.4623271698674506E-5 1.2605370392001808E-4 62-63 3.7675498269282643 2.41602932513368E-4 0.0 5.4623271698674506E-5 1.2815459898535172E-4 64-65 4.246745083275279 2.5210740784003616E-4 0.0 5.4623271698674506E-5 1.3025549405068536E-4 66-67 4.752932639421699 2.584100930360371E-4 0.0 5.672416676400814E-5 1.3025549405068536E-4 68-69 5.282969556349787 2.626118831667043E-4 0.0 5.882506182934177E-5 1.32356389116019E-4 70-71 5.836007072453148 2.6471277823203796E-4 0.0 5.882506182934177E-5 1.3445728418135262E-4 72-73 6.413167065696669 2.6891456836270524E-4 0.0 6.0925956894675405E-5 1.3445728418135262E-4 74-75 7.017439109758321 2.773181486240398E-4 0.0 6.722864209067631E-5 1.386590743120199E-4 76-77 7.646949206239826 2.9202441408137525E-4 0.0 6.722864209067631E-5 1.386590743120199E-4 78-79 8.292232822871856 2.983270992773762E-4 0.0 7.143043222134358E-5 1.4286086444268716E-4 80-81 8.956609373857638 3.025288894080434E-4 0.0 7.143043222134358E-5 1.449617595080208E-4 82-83 9.639919191172208 3.025288894080434E-4 0.0 7.563222235201085E-5 1.4706265457335445E-4 84-85 10.343296758150842 3.109324696693779E-4 0.0 7.563222235201085E-5 1.512644447040217E-4 86-87 11.06501093725971 3.1723515486537884E-4 2.1008950653336347E-6 7.983401248267812E-5 1.512644447040217E-4 88 11.617853070121996 3.2353784006137977E-4 4.201790130667269E-6 7.983401248267812E-5 1.512644447040217E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 149100 0.0 87.575615 8 GTGGTAT 152235 0.0 86.03147 6 TGGTATC 152070 0.0 85.85595 7 GTATCAA 152165 0.0 85.77146 9 AGTGGTA 152960 0.0 85.706665 5 GCAGTGG 159845 0.0 82.238464 3 AAGCAGT 156810 0.0 81.70366 1 CAGTGGT 161595 0.0 81.045395 4 AGCAGTG 164430 0.0 79.862434 2 CAACGCA 145875 0.0 45.50818 12-13 ATCAACG 147225 0.0 45.117973 10-11 ACGCAGA 148620 0.0 44.8151 14-15 TCAACGC 145455 0.0 44.801144 12-13 AACGCAG 147495 0.0 44.350662 14-15 AGAGTAC 150565 0.0 43.997456 18-19 CGCAGAG 149485 0.0 43.818466 16-17 GAGTACT 148720 0.0 43.47326 20-21 TATCAAC 151710 0.0 42.99267 10-11 AGTACTT 157790 0.0 41.68029 20-21 GTACTTT 155735 0.0 41.388206 22-23 >>END_MODULE