FastQCFastQC Report
Thu 26 May 2016
SRR1660767_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660767_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26903416
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15213275.654772613262197No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1082150.4022351659729753Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA898000.33378660910569874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1892300.087.1185468
GTATCAA1911750.086.183079
AGTGGTA1910650.086.087655
GTGGTAT1919500.085.866916
TGGTATC1915550.085.786527
AAGCAGT2015000.082.636351
GCAGTGG2011550.082.5589453
CAGTGGT2005650.082.045284
AGCAGTG2071000.080.356112
CAACGCA1882450.044.9247812-13
ATCAACG1896250.044.73902510-11
ACGCAGA1902300.044.45016514-15
TCAACGC1883350.044.33346612-13
AACGCAG1896200.044.0281114-15
AGAGTAC1893400.044.0055618-19
GAGTACT1850450.043.97095520-21
CGCAGAG1918700.043.47070716-17
AGTACTT1923300.042.9632120-21
TATCAAC1929150.042.8767910-11
GTACTTT1938100.041.98576722-23
Produced by FastQC (version 0.11.5)