##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660767_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26903416 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17995313308912 31.0 31.0 34.0 27.0 34.0 2 31.326513629347293 31.0 31.0 34.0 27.0 34.0 3 31.36651096648842 33.0 31.0 34.0 27.0 34.0 4 34.8258944886404 37.0 35.0 37.0 32.0 37.0 5 34.811327825433025 37.0 35.0 37.0 32.0 37.0 6 34.792364620165706 37.0 35.0 37.0 31.0 37.0 7 34.752446232106735 37.0 35.0 37.0 31.0 37.0 8 34.78935656349365 37.0 35.0 37.0 31.0 37.0 9 36.25728439094872 39.0 37.0 39.0 31.0 39.0 10-11 36.197941610834846 39.0 35.0 39.0 30.5 39.0 12-13 36.34332099314079 39.0 37.0 39.0 31.0 39.0 14-15 37.527089162208995 40.0 37.0 41.0 31.0 41.0 16-17 37.482298214472095 40.0 37.0 41.0 31.0 41.0 18-19 37.439618950247805 40.0 37.0 41.0 31.0 41.0 20-21 37.38516755641737 40.0 37.0 41.0 31.0 41.0 22-23 37.21174186579132 40.0 36.5 41.0 30.5 41.0 24-25 37.24815506699967 40.0 36.5 41.0 31.0 41.0 26-27 37.16896016104424 40.0 36.0 41.0 30.0 41.0 28-29 37.15356347312921 40.0 36.0 41.0 30.0 41.0 30-31 37.02976413850197 40.0 36.0 41.0 30.0 41.0 32-33 36.91001668710025 40.0 36.0 41.0 30.0 41.0 34-35 36.7750806997892 40.0 36.0 41.0 30.0 41.0 36-37 36.54687598035878 39.0 35.0 40.5 29.5 41.0 38-39 36.099485674978965 38.0 35.0 40.0 29.0 41.0 40-41 35.95000807704122 38.0 35.0 40.0 28.0 41.0 42-43 36.155110878856426 39.0 35.0 40.0 29.0 41.0 44-45 36.214179771817825 39.0 35.0 41.0 28.5 41.0 46-47 36.1178409277097 39.0 35.0 41.0 28.0 41.0 48-49 36.003091261719334 39.0 35.0 41.0 28.0 41.0 50-51 34.90472538877591 38.0 33.5 40.0 25.5 40.5 52-53 34.89421467890918 38.0 33.5 39.5 26.0 40.5 54-55 35.07490859153351 38.0 34.0 40.0 23.5 41.0 56-57 34.70519464888771 38.0 33.0 41.0 22.0 41.0 58-59 34.39722327454625 38.0 33.0 40.0 21.0 41.0 60-61 34.168002048513095 37.5 33.0 40.0 20.0 41.0 62-63 33.88287788435491 37.0 33.0 40.0 20.0 41.0 64-65 33.62140114474683 36.5 32.0 40.0 20.0 41.0 66-67 33.315964597209515 36.0 32.0 39.5 19.5 41.0 68-69 33.0316054288422 35.5 32.0 39.0 19.0 41.0 70-71 32.75530159441463 35.0 31.0 39.0 19.0 41.0 72-73 32.42677502366242 35.0 31.0 38.0 19.0 40.0 74-75 32.098095331091045 35.0 31.0 37.0 18.0 40.0 76-77 31.705841481245358 35.0 31.0 37.0 17.5 39.0 78-79 31.337853044386634 35.0 30.0 36.0 17.0 39.0 80-81 31.037860340857833 35.0 30.0 36.0 15.0 38.0 82-83 30.744550580491342 35.0 30.0 35.0 15.0 37.0 84-85 30.477116363215735 34.0 29.5 35.0 12.5 37.0 86-87 30.258716551087787 34.0 29.0 35.0 12.0 36.0 88-89 30.051704289150493 34.0 29.0 35.0 11.0 36.0 90-91 29.87455710085292 34.0 29.0 35.0 10.0 36.0 92-93 29.714713235672377 34.0 29.0 35.0 10.0 35.5 94-95 29.55434941793265 34.0 29.0 35.0 10.0 35.0 96-97 29.44115946093983 34.0 29.0 35.0 10.0 35.0 98-99 29.304461838600716 34.0 28.0 35.0 10.0 35.0 100 29.203171411392518 34.0 27.0 35.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0825430822321884 1101 2 1.0449470009917903 1101 3 1.0618917371818988 1101 4 1.0200412547211428 1101 5 1.0273862213095555 1101 6 1.0250189382626687 1101 7 0.9899559354062006 1101 8 1.0200118577513706 1101 9 1.3395235500852252 1101 10-11 1.2339471011029346 1101 12-13 1.1609582459001118 1101 14-15 1.3701425584831384 1101 16-17 1.3941836315743217 1101 18-19 1.3679426723471622 1101 20-21 1.3257260468343475 1101 22-23 1.298250301463753 1101 24-25 1.2401634172386338 1101 26-27 1.3566100500438054 1101 28-29 1.2452853046747947 1101 30-31 1.1624573409945356 1101 32-33 1.1926969653640924 1101 34-35 1.226305424595175 1101 36-37 1.2547609525042205 1101 38-39 1.2514064243133873 1101 40-41 1.2087394433320569 1101 42-43 1.308377274871937 1101 44-45 1.3001013940691948 1101 46-47 1.3053817976832534 1101 48-49 1.3746902579904798 1101 50-51 1.2386358045246055 1101 52-53 1.3129754823495219 1101 54-55 1.404824699667465 1101 56-57 1.3316713377827156 1101 58-59 1.307986437567159 1101 60-61 1.2796236153190748 1101 62-63 1.2512827492209055 1101 64-65 1.1527077563965875 1101 66-67 1.1499439739733575 1101 68-69 1.0832457366012846 1101 70-71 1.0514534523272019 1101 72-73 1.051299368197121 1101 74-75 1.0322524552832917 1101 76-77 1.0172929285689172 1101 78-79 1.0058741194884746 1101 80-81 1.0130508373416838 1101 82-83 0.9533709036347489 1101 84-85 0.9172126428411111 1101 86-87 0.8807377601148367 1101 88-89 0.9143621957899946 1101 90-91 0.8558814364723197 1101 92-93 0.8837419424650079 1101 94-95 0.845262163779708 1101 96-97 0.839612811739638 1101 98-99 0.7958566179053896 1101 100 0.6251241090449255 1102 1 1.025080675862231 1102 2 1.0152486670872918 1102 3 0.9781272711895888 1102 4 0.9409387302062981 1102 5 0.9751974760380335 1102 6 1.025490429131004 1102 7 0.9779126459788827 1102 8 0.988957064503893 1102 9 1.1639830967912843 1102 10-11 1.1606013064275302 1102 12-13 1.0674730289399719 1102 14-15 1.3330909391721875 1102 16-17 1.2950085419464088 1102 18-19 1.3126653680125955 1102 20-21 1.220937550515238 1102 22-23 1.200423526631269 1102 24-25 1.1190657265752293 1102 26-27 1.2545700016426267 1102 28-29 1.1866458821647825 1102 30-31 1.1626628254513562 1102 32-33 1.1473040924186648 1102 34-35 1.1719029157304774 1102 36-37 1.1360983443493637 1102 38-39 1.1316886876677898 1102 40-41 1.0722681604087398 1102 42-43 1.1959781289251197 1102 44-45 1.2281198551392194 1102 46-47 1.2060605311411905 1102 48-49 1.2455340023983936 1102 50-51 1.0922127517810694 1102 52-53 1.1639850806281586 1102 54-55 1.328001077718973 1102 56-57 1.2655218683169451 1102 58-59 1.2328864758917888 1102 60-61 1.2382146599948172 1102 62-63 1.235680406171582 1102 64-65 1.2057864650010615 1102 66-67 1.138709474775787 1102 68-69 1.089805822534963 1102 70-71 1.1083666014278748 1102 72-73 1.0645830904433495 1102 74-75 0.9649397572476133 1102 76-77 0.9821819489201253 1102 78-79 1.0111010902324509 1102 80-81 1.0379053761729047 1102 82-83 0.9917906492455266 1102 84-85 0.9468300770387152 1102 86-87 0.9069532759837635 1102 88-89 0.8788813222062757 1102 90-91 0.9099752563355672 1102 92-93 0.8818972024440015 1102 94-95 0.818155193697617 1102 96-97 0.8870100338775551 1102 98-99 0.8740188598320806 1102 100 0.8379031662900971 1103 1 0.9361772905113348 1103 2 0.9658058086729113 1103 3 0.951134167571059 1103 4 0.9422116526631399 1103 5 0.9278678261977404 1103 6 0.9941779596614992 1103 7 0.9195296749288673 1103 8 0.9349411364494387 1103 9 1.1471956788239694 1103 10-11 1.139484108016383 1103 12-13 1.0419309102026304 1103 14-15 1.253253759755296 1103 16-17 1.2733523864325562 1103 18-19 1.2458763975671516 1103 20-21 1.1558441492410765 1103 22-23 1.105965564528617 1103 24-25 1.0916711736691909 1103 26-27 1.1443978236363037 1103 28-29 1.086132720793259 1103 30-31 1.0675940290181387 1103 32-33 1.0818306892649119 1103 34-35 1.1157521567073232 1103 36-37 1.1158170035459847 1103 38-39 1.0842612712217345 1103 40-41 1.0868030284723176 1103 42-43 1.1649953190086393 1103 44-45 1.1426225646861141 1103 46-47 1.1199693771827128 1103 48-49 1.1249021936239672 1103 50-51 1.1168016324114234 1103 52-53 1.131668516341115 1103 54-55 1.1823922812815653 1103 56-57 1.2200497436044557 1103 58-59 1.2067641099215862 1103 60-61 1.1717911983000349 1103 62-63 1.1229617746440042 1103 64-65 1.0995479273628135 1103 66-67 1.0552739275786465 1103 68-69 1.0432219573425456 1103 70-71 0.9609382250548109 1103 72-73 1.0002824558221732 1103 74-75 0.9541860132625359 1103 76-77 0.9522627898703426 1103 78-79 1.0127180230743633 1103 80-81 0.9626903886231055 1103 82-83 0.9032105229727065 1103 84-85 0.8014098749172724 1103 86-87 0.7736040311677925 1103 88-89 0.7975320058265147 1103 90-91 0.822103918484796 1103 92-93 0.8993655494129271 1103 94-95 0.8737357470322031 1103 96-97 0.8551446756721823 1103 98-99 0.795509640033977 1103 100 0.7366117785063153 1104 1 0.9380171591879041 1104 2 0.9055930780936947 1104 3 0.9568206105645736 1104 4 0.906585977234009 1104 5 0.9409895675637756 1104 6 0.9691182902917603 1104 7 0.9365870481474019 1104 8 0.9129857737836602 1104 9 1.1581104095182368 1104 10-11 1.09308677833301 1104 12-13 1.0021358577872377 1104 14-15 1.2157016560407001 1104 16-17 1.2531576777672413 1104 18-19 1.2435646697321872 1104 20-21 1.1652742211210878 1104 22-23 1.1357708146371337 1104 24-25 1.0274228616952286 1104 26-27 1.143736475676441 1104 28-29 1.0970342771570927 1104 30-31 1.0768873734678195 1104 32-33 1.109308880276778 1104 34-35 1.0885773595918735 1104 36-37 1.05961443667222 1104 38-39 1.1092736026710668 1104 40-41 1.127266880228646 1104 42-43 1.1415733576556306 1104 44-45 1.1198937486634932 1104 46-47 1.101968662726975 1104 48-49 1.1543871362835247 1104 50-51 1.1446739320207726 1104 52-53 1.1638608993744626 1104 54-55 1.2534940560387042 1104 56-57 1.2303541358235677 1104 58-59 1.229221158920602 1104 60-61 1.2539317656381215 1104 62-63 1.2584574889659308 1104 64-65 1.1717282002239742 1104 66-67 1.064660380011965 1104 68-69 1.1154542789125017 1104 70-71 1.073264691703514 1104 72-73 1.04750401560419 1104 74-75 1.0147081980745085 1104 76-77 0.9963616165670395 1104 78-79 1.0315069808860784 1104 80-81 0.9732180245804223 1104 82-83 0.9395951973423067 1104 84-85 0.8959146118098467 1104 86-87 0.8734559846746315 1104 88-89 0.9018751268279566 1104 90-91 0.833199557164928 1104 92-93 0.8596173793552992 1104 94-95 0.9054048906576249 1104 96-97 0.9194590288939146 1104 98-99 0.9599359305004604 1104 100 0.9782151838646911 1105 1 0.7008249206653616 1105 2 0.7234046710417559 1105 3 0.7394043843658444 1105 4 0.7174481708189475 1105 5 0.6955326155823514 1105 6 0.764556620716796 1105 7 0.807275268346018 1105 8 0.8082772078123384 1105 9 0.987751927900689 1105 10-11 0.9168774500199532 1105 12-13 0.8209304123007186 1105 14-15 1.008300203732162 1105 16-17 0.9688940039468861 1105 18-19 0.9320844730873432 1105 20-21 0.9289446798407326 1105 22-23 0.9126918109869848 1105 24-25 0.8056951145725932 1105 26-27 0.9583520108997234 1105 28-29 0.9316513225129839 1105 30-31 0.8624577013658197 1105 32-33 0.8357886294034316 1105 34-35 0.8680012597750277 1105 36-37 0.8965902913837027 1105 38-39 0.8946961077521536 1105 40-41 0.832317880266892 1105 42-43 0.8979959669422541 1105 44-45 0.9159051809615093 1105 46-47 0.9793196541505154 1105 48-49 0.9718662222978267 1105 50-51 0.9129824698705278 1105 52-53 0.9839596433848428 1105 54-55 1.0581457520403106 1105 56-57 1.0456731573804845 1105 58-59 0.9613768475513993 1105 60-61 1.0240275575221744 1105 62-63 0.9816470698027615 1105 64-65 0.9289203318389312 1105 66-67 0.8639014494228334 1105 68-69 0.8855429279599107 1105 70-71 0.8832753361412742 1105 72-73 0.9019231062046131 1105 74-75 0.8529519876876961 1105 76-77 0.8698035983309573 1105 78-79 0.8426020702177794 1105 80-81 0.9282589212642058 1105 82-83 0.8547065504901106 1105 84-85 0.8195449380587441 1105 86-87 0.8394110744920233 1105 88-89 0.7773384541603114 1105 90-91 0.7883052974126166 1105 92-93 0.8151016270089215 1105 94-95 0.8248021027124821 1105 96-97 0.7947459033223616 1105 98-99 0.8499035319629371 1105 100 0.8707308689448787 1106 1 0.6651609462154155 1106 2 0.6780182643296513 1106 3 0.6477505764544453 1106 4 0.6888205368142124 1106 5 0.6812286418443634 1106 6 0.7375455977695964 1106 7 0.7229348780711362 1106 8 0.7265476367892774 1106 9 0.883101851561122 1106 10-11 0.8516082774641873 1106 12-13 0.7809760355924453 1106 14-15 0.8165318210240713 1106 16-17 0.8451876603178974 1106 18-19 0.8651614812264441 1106 20-21 0.8402249545763638 1106 22-23 0.8321378179643233 1106 24-25 0.7326063129925728 1106 26-27 0.8221032929645062 1106 28-29 0.7516674797549143 1106 30-31 0.6987517278792907 1106 32-33 0.696319002286863 1106 34-35 0.6899843808476689 1106 36-37 0.7547074924674106 1106 38-39 0.7670806701878803 1106 40-41 0.7775168938895902 1106 42-43 0.6964164963857797 1106 44-45 0.7430764152384981 1106 46-47 0.7427630466656865 1106 48-49 0.7198264293965408 1106 50-51 0.7358418755061109 1106 52-53 0.7605807187854197 1106 54-55 0.8330368163732587 1106 56-57 0.8780895258930812 1106 58-59 0.943662574559383 1106 60-61 0.9497771783802023 1106 62-63 0.8772190358286167 1106 64-65 0.8974870657070895 1106 66-67 0.8643317662090411 1106 68-69 0.8644074469811827 1106 70-71 0.8703655939023918 1106 72-73 0.7847888421385107 1106 74-75 0.7453240398009271 1106 76-77 0.7789016538420377 1106 78-79 0.8459211041040717 1106 80-81 0.8096584157456839 1106 82-83 0.7691215592680578 1106 84-85 0.7395104563014563 1106 86-87 0.6342168579402703 1106 88-89 0.7003288277156585 1106 90-91 0.7585928811685143 1106 92-93 0.7181271536281884 1106 94-95 0.7411737763073099 1106 96-97 0.6890971860091817 1106 98-99 0.7077909598771548 1106 100 0.755516511038735 1107 1 0.775776887374068 1107 2 0.811512080832749 1107 3 0.8208536415178109 1107 4 0.8390803827749451 1107 5 0.7443532410518969 1107 6 0.8144800782796153 1107 7 0.8385406382073128 1107 8 0.8561713982291437 1107 9 1.0500257883399726 1107 10-11 1.0271490445100895 1107 12-13 0.9120701426200384 1107 14-15 1.1445119500018208 1107 16-17 1.1754823421751723 1107 18-19 1.1725055304044503 1107 20-21 1.099396316186514 1107 22-23 1.048611358309259 1107 24-25 1.012728680528241 1107 26-27 1.1216761512433848 1107 28-29 1.0818659874724403 1107 30-31 1.034379498930818 1107 32-33 1.006099581033446 1107 34-35 1.018855275327823 1107 36-37 1.0979941985357442 1107 38-39 1.10526957087162 1107 40-41 1.1294770428688068 1107 42-43 1.1599261238903793 1107 44-45 1.10319907699499 1107 46-47 1.1391498947254775 1107 48-49 1.1170842189337478 1107 50-51 1.071337462785472 1107 52-53 1.0824895217616586 1107 54-55 1.1350722790218484 1107 56-57 1.1541702142731225 1107 58-59 1.1404978396406378 1107 60-61 1.1259937877789312 1107 62-63 1.0835140558950513 1107 64-65 1.0569581133205546 1107 66-67 1.0375572580425185 1107 68-69 1.0046590890028 1107 70-71 1.0214095062532422 1107 72-73 1.0355604683510151 1107 74-75 0.9758798627822713 1107 76-77 0.986279855359907 1107 78-79 1.0494056244503582 1107 80-81 1.0289532278557836 1107 82-83 0.9276820645654453 1107 84-85 0.9433750748260898 1107 86-87 0.9595383203131931 1107 88-89 0.9572630016040193 1107 90-91 0.9237291683580473 1107 92-93 0.9042302845607573 1107 94-95 0.9096813516311144 1107 96-97 0.9586381131454509 1107 98-99 1.0092507751417266 1107 100 1.0485403231847954 1108 1 0.4518034693333455 1108 2 0.4230374603575342 1108 3 0.44330922563149144 1108 4 0.5005232847268388 1108 5 0.4860928710363126 1108 6 0.5170811172397478 1108 7 0.47415257317508974 1108 8 0.5308458863404297 1108 9 0.6440900349611312 1108 10-11 0.5953529383159335 1108 12-13 0.5394064832215193 1108 14-15 0.5966922600968587 1108 16-17 0.6114928662042729 1108 18-19 0.5580092630138154 1108 20-21 0.5240982769625688 1108 22-23 0.5302930964854085 1108 24-25 0.4525506673203523 1108 26-27 0.4464387403064123 1108 28-29 0.42283647987537876 1108 30-31 0.478944257071646 1108 32-33 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-0.7376182274030327 2314 30-31 -0.6150813143399816 2314 32-33 -0.7944049414123029 2314 34-35 -0.6815471756289284 2314 36-37 -0.7352988091096364 2314 38-39 -0.8284245492042857 2314 40-41 -0.6690650760306127 2314 42-43 -0.7581331788491497 2314 44-45 -0.671252938346818 2314 46-47 -0.5999785022058859 2314 48-49 -0.6651595703045743 2314 50-51 -0.6942097519985211 2314 52-53 -0.696227494647431 2314 54-55 -0.8798733967583914 2314 56-57 -0.889732725644599 2314 58-59 -0.7126641825764111 2314 60-61 -0.6319770250805661 2314 62-63 -0.619309941912249 2314 64-65 -0.6135371963417313 2314 66-67 -0.5420657167938998 2314 68-69 -0.5297710631530563 2314 70-71 -0.51032371501028 2314 72-73 -0.5649725243702406 2314 74-75 -0.5198717015205361 2314 76-77 -0.48444662044059683 2314 78-79 -0.48697295872229773 2314 80-81 -0.43230457105395814 2314 82-83 -0.46589692319670206 2314 84-85 -0.5628132488107589 2314 86-87 -0.4766301341872001 2314 88-89 -0.45178659026072054 2314 90-91 -0.4512147199134837 2314 92-93 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50-51 -1.487643655726906 2315 52-53 -1.8136411575410918 2315 54-55 -2.0408289927092014 2315 56-57 -1.977387990053849 2315 58-59 -2.1528031005817283 2315 60-61 -1.988577192357269 2315 62-63 -1.9095265899647273 2315 64-65 -1.7441539333798701 2315 66-67 -1.6151507250922208 2315 68-69 -1.9339077409045764 2315 70-71 -1.85142170099396 2315 72-73 -1.8932669677053333 2315 74-75 -2.0284364097681262 2315 76-77 -1.8220873812888492 2315 78-79 -1.6688696128180425 2315 80-81 -1.8827579021201828 2315 82-83 -1.7286400230856813 2315 84-85 -1.4440492956140325 2315 86-87 -1.4905471360850875 2315 88-89 -1.4082895339598274 2315 90-91 -1.5337708412234257 2315 92-93 -1.3688261987002477 2315 94-95 -1.4501094662549825 2315 96-97 -1.2876771649255225 2315 98-99 -1.2892144518988324 2315 100 -1.241338170505827 2316 1 -4.528009806459519 2316 2 -3.311210081938672 2316 3 -4.0993362770159685 2316 4 -3.4366985945384236 2316 5 -2.8275154432601965 2316 6 -5.640388020268354 2316 7 -5.57420081780829 2316 8 -5.34178439640425 2316 9 -4.0773665497512255 2316 10-11 -5.228641693005045 2316 12-13 -4.225420547436194 2316 14-15 -4.324856877274385 2316 16-17 -5.509290541843697 2316 18-19 -3.9572662153004927 2316 20-21 -3.0553470861277887 2316 22-23 -3.717093562299439 2316 24-25 -2.84775433991382 2316 26-27 -3.7747619379112862 2316 28-29 -3.2420100198414445 2316 30-31 -3.0314794017987055 2316 32-33 -2.796460504492778 2316 34-35 -2.6785781334151224 2316 36-37 -2.6912556800095473 2316 38-39 -2.8843123353401268 2316 40-41 -2.772063790617999 2316 42-43 -2.9775414370779885 2316 44-45 -2.8993983562852534 2316 46-47 -3.323386695016424 2316 48-49 -3.1432484257243303 2316 50-51 -2.915459331959177 2316 52-53 -3.0145035499643384 2316 54-55 -3.0621662925308755 2316 56-57 -3.071414134249359 2316 58-59 -3.287736223402817 2316 60-61 -3.4500527634948206 2316 62-63 -3.0455507679196074 2316 64-65 -2.7455450241153834 2316 66-67 -2.8819870706360327 2316 68-69 -2.9475545309935853 2316 70-71 -2.8462880295465283 2316 72-73 -2.881709068104925 2316 74-75 -2.5543035482930208 2316 76-77 -2.5408860141918623 2316 78-79 -5.573194117203563 2316 80-81 -3.1807355254557095 2316 82-83 -2.380730306468834 2316 84-85 -2.226091271465638 2316 86-87 -2.318087744661444 2316 88-89 -2.1456472239752067 2316 90-91 -2.1916897203711265 2316 92-93 -2.1885462313511894 2316 94-95 -2.1414107008860377 2316 96-97 -2.172134181567589 2316 98-99 -2.241565464622301 2316 100 -2.2312629102573673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 69.0 5 227.0 6 772.0 7 6876.0 8 9325.0 9 17560.0 10 24223.0 11 29626.0 12 37955.0 13 48542.0 14 63955.0 15 83436.0 16 106379.0 17 129790.0 18 148446.0 19 169645.0 20 195999.0 21 239165.0 22 304797.0 23 408729.0 24 531661.0 25 608806.0 26 603854.0 27 555548.0 28 513722.0 29 541005.0 30 615634.0 31 726258.0 32 879505.0 33 1097570.0 34 1430702.0 35 1994780.0 36 3008135.0 37 4839120.0 38 5404961.0 39 1523345.0 40 3284.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.133550149947638 35.39469627255591 26.778195443430185 19.69355813406626 2 21.824824269467907 33.41048906553186 25.567615826907865 19.19707083809237 3 26.833726494434078 26.022759704708022 25.82086365491213 21.322650145945772 4 20.46940361915806 26.35809865760479 25.703830183989496 27.468667539247654 5 20.946724029913685 32.469927133758716 25.36748043602659 21.215868400301 6 28.17934718773259 25.855337478333606 25.31082298247925 20.65449235145455 7 21.266589343152557 25.950879248939984 32.08078855116391 20.701742856743547 8 27.332592262633113 26.58276183217774 25.401859748962735 20.682786156226406 9 27.20216728408051 26.310531079234785 25.684579269294687 20.80272236739002 10-11 20.64934058931401 29.872448167920385 28.86776162551254 20.610449617253064 12-13 20.682512956718956 26.248633259062714 28.865423632448756 24.203430151769574 14-15 20.66179068115365 33.00263802931197 25.711002647396153 20.624568642138232 16-17 24.126928714182615 26.18698495388095 25.446303919175172 24.239782412761265 18-19 20.74806782900729 29.579704673934344 25.64379371006269 24.028433786995674 20-21 23.944034606785248 29.847651242257427 25.607182402239797 20.601131748717524 22-23 24.369452839043333 26.345038295942206 28.715373709353088 20.57013515566137 24-25 20.796870716133085 29.878798263215472 25.27095305693024 24.053377963721196 26-27 20.62702371266961 26.76808903517709 31.973384785399077 20.63150246675422 28-29 20.820627813028782 26.62890639205514 31.98093709011427 20.569528704801808 30-31 20.844021317976818 26.364113551657436 32.17855443479337 20.613310695572373 32-33 20.835108667558785 26.416971415591366 32.14236991251245 20.6055500043374 34-35 20.8448779998432 26.727632086822002 31.867566840326017 20.559923073008786 36-37 20.62144863803526 26.703213318665142 32.04038037169773 20.634957671601864 38-39 20.639315553380396 26.445373019763135 32.0289529800896 20.886358446766877 40-41 20.902896787692 26.545368430888317 31.75215653651962 20.79957824490006 42-43 20.903836228392798 26.58079376334777 31.96522360955738 20.550146398702047 44-45 21.18537471181047 26.617599527048412 31.661038766268057 20.535986994873053 46-47 20.726668283999025 26.71537778684188 31.814773905285126 20.74318002387397 48-49 21.037936219112886 26.719561625499338 31.444011740182237 20.798490415205535 50-51 21.075494001126106 26.77844959646575 31.369826676479057 20.77622972592908 52-53 20.929939086972357 27.21042478794512 31.353390372091937 20.506245752990587 54-55 21.322631403347064 27.24110884674747 30.517105339794064 20.919154410111396 56-57 21.778874787601833 27.99661118478195 29.38394738412998 20.840566643486238 58-59 21.96134278404752 28.541786361825118 28.684594154561154 20.81227669956621 60-61 22.067999473116508 29.08046129419449 28.028267600501884 20.823271632187122 62-63 22.18909889840543 29.243367299493023 27.812447895687555 20.75508590641399 64-65 22.280224856292296 29.281401014499252 27.821772119300885 20.616602009907563 66-67 22.413528759680773 28.994957760341972 27.994730143744455 20.596783336232797 68-69 22.53506585547328 28.7032690494338 28.264117888188693 20.497547206904223 70-71 22.669747777989645 28.509994216314738 28.479920986922608 20.34033701877301 72-73 22.815126560633185 28.39409352904739 28.499767989265155 20.291011921054277 74-75 23.058023946081136 28.393735904086558 28.35888880348024 20.189351346352066 76-77 23.37820967408395 28.482061851061612 28.04377052154612 20.095957953308314 78-79 23.652916709361094 28.561499320040713 27.666929071212664 20.11865489938553 80-81 23.915079689483992 28.69997009442335 27.303953234631607 20.080996981461052 82-83 24.08441387249793 28.86938004053811 27.02368367331382 20.022522413650137 84-85 24.273894289143204 28.92406755383634 26.795006118123005 20.007032038897453 86-87 24.477771232864026 28.925716972698513 26.6195119246374 19.976999869800057 88-89 24.690040647288217 28.93509244893082 26.469020838613528 19.905846065167434 90-91 24.89849180762439 28.88270160717632 26.295039024762684 19.923767560436605 92-93 25.03823146568815 28.8637729631448 26.207417778269328 19.890577792897727 94-95 25.186393284642516 28.898285651125594 26.143749672723466 19.771571391508424 96-97 25.35033328536567 28.857363659084452 26.031050726609646 19.761252328940234 98-99 25.459876736025894 28.833703705356413 25.962078515894017 19.744341042723676 100 25.557524500032724 28.883615919219807 25.84897373572691 19.70988584502056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52987.0 1 33822.5 2 12606.0 3 10297.0 4 9931.5 5 10035.0 6 10501.0 7 10892.5 8 11190.0 9 11535.0 10 12525.0 11 15377.0 12 97650.0 13 123509.5 14 69409.5 15 73893.5 16 83319.0 17 92825.5 18 101493.5 19 110148.0 20 120159.0 21 134210.0 22 152756.5 23 174141.5 24 197118.5 25 223386.0 26 253893.5 27 289194.5 28 330378.5 29 371428.5 30 407455.5 31 444003.0 32 483892.0 33 521355.0 34 556499.0 35 595138.0 36 638273.5 37 680454.5 38 722680.0 39 754198.5 40 773292.5 41 799140.5 42 830292.5 43 844103.0 44 849787.0 45 867908.5 46 884309.5 47 895501.0 48 904200.0 49 906149.0 50 900941.5 51 894411.5 52 883028.5 53 849402.0 54 803676.0 55 757671.5 56 705842.5 57 643367.5 58 571620.0 59 495234.0 60 412979.0 61 335755.5 62 267217.0 63 205201.5 64 155098.0 65 116753.0 66 86817.5 67 64818.0 68 48667.5 69 37182.5 70 29185.5 71 23367.5 72 19362.5 73 15284.0 74 11799.5 75 9605.0 76 7511.5 77 5217.5 78 3522.0 79 2485.5 80 1781.5 81 1338.5 82 1017.5 83 747.5 84 546.5 85 423.5 86 349.0 87 284.0 88 233.5 89 191.0 90 160.0 91 127.0 92 96.0 93 82.5 94 63.5 95 49.5 96 48.0 97 40.5 98 29.5 99 17.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03727407701683682 2 0.013782636375990321 3 0.003560886097140973 4 3.2337900882177935E-4 5 4.088700111539739E-5 6 0.0 7 0.0 8 0.0 9 2.490390067937841E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.05421244647891554 22-23 0.07271381448363286 24-25 0.048018065809932835 26-27 0.03633739299128408 28-29 0.03809925103934757 30-31 0.03389532392466444 32-33 0.035010423955084365 34-35 0.03666634750025796 36-37 0.03828695954446826 38-39 0.05187445341513509 40-41 0.07273425798419056 42-43 0.08632918585506019 44-45 0.08183904973256928 46-47 0.05002152886458731 48-49 0.03197921037239286 50-51 0.020261367552730107 52-53 0.0298103408132261 54-55 0.02864506128143727 56-57 0.029291819299080833 58-59 0.035592134470953427 60-61 0.06325033222546907 62-63 0.09382637505958351 64-65 0.1190592302479358 66-67 0.09249383052323171 68-69 0.056847799550807966 70-71 0.05841265659349727 72-73 0.05817662708705838 74-75 0.04864438032701869 76-77 0.05371622696537867 78-79 0.04360970368967272 80-81 0.038799905558461426 82-83 0.03793570303488598 84-85 0.050673862382382966 86-87 0.05204915241990087 88-89 0.06089375416118161 90-91 0.05280742044058643 92-93 0.049294855344763654 94-95 0.055160281504772486 96-97 0.051188666896426836 98-99 0.04287931316974766 100 0.050759353384715156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6903416E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.371803259275495 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36662049425811 35.70252718370476 2 12.684218358681655 12.017485931703153 3 4.410077854283561 6.267400214136259 4 2.1626352482007416 4.097917259973598 5 1.2772096561267248 3.025186227544106 6 0.829304505472748 2.357138992517147 7 0.5933444971758618 1.9675459149632066 8 0.40146544718853955 1.5214513743690044 9 0.3313550962465198 1.412719958551359 >10 1.7204856872233376 14.684003027075542 >50 0.1587423449351253 5.224021680535643 >100 0.06352586055197565 4.532837673511539 >500 4.747501000229979E-4 0.14625488119049554 >1k 4.777076393766072E-4 0.49064234566868253 >5k 2.343446843111126E-5 0.07253979249319825 >10k+ 3.9057447384918204E-5 6.48032754206226 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1521327 5.654772613262197 No Hit AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 108215 0.4022351659729753 Illumina Single End PCR Primer 1 (100% over 50bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89800 0.33378660910569874 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.4856483652484874 1.8585000506998813E-5 0.0 0.0 7.434000202799525E-6 2 0.485700403249907 2.97360008111981E-5 0.0 7.434000202799525E-6 7.434000202799525E-6 3 0.485793328252442 3.3453000912597864E-5 0.0 7.434000202799525E-6 7.434000202799525E-6 4 0.4858528002540644 3.717000101399763E-5 0.0 7.434000202799525E-6 7.434000202799525E-6 5 0.485904838255484 4.8321001318196915E-5 0.0 1.1151000304199288E-5 1.1151000304199288E-5 6 0.4859457252565994 5.94720016223962E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 7 0.48599776325801897 6.318900172379597E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 8 0.48608325426035115 6.318900172379597E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 9 0.4861241412614666 7.06230019265955E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 10-11 0.48619104726329176 8.177400223079479E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 12-13 0.48630999126653657 8.92080024335943E-5 0.0 2.601900070979834E-5 1.486800040559905E-5 14-15 0.48646982227089675 1.0035900273779359E-4 0.0 3.3453000912597864E-5 1.486800040559905E-5 16-17 0.48660549277459786 1.1151000304199288E-4 0.0 3.531150096329774E-5 1.486800040559905E-5 18-19 0.48676346527890735 1.3381200365039148E-4 0.0 3.902850106469751E-5 1.8585000506998813E-5 20-21 0.48691772078311546 1.505385041066904E-4 0.0 4.460400121679715E-5 2.4160500659098458E-5 22-23 0.48716861828995994 1.784160048671886E-4 0.0 4.460400121679715E-5 2.601900070979834E-5 24-25 0.4877763478065388 1.932840052727877E-4 0.0 5.203800141959668E-5 2.601900070979834E-5 26-27 0.489731489859875 2.2859550623608542E-4 0.0 5.761350157169632E-5 2.601900070979834E-5 28-29 0.4951508760077159 2.490390067937841E-4 0.0 5.94720016223962E-5 4.274550116609727E-5 30-31 0.5085841143741746 2.694825073514828E-4 0.0 6.504750177449586E-5 5.389650147029656E-5 32-33 0.5339303380656196 2.9178450795988136E-4 0.0 7.434000202799525E-5 5.94720016223962E-5 34-35 0.5782165357737471 3.0293550826408067E-4 0.0 7.434000202799525E-5 6.504750177449586E-5 36-37 0.6454830866087786 3.196620087203796E-4 0.0 7.619850207869514E-5 7.434000202799525E-5 38-39 0.7414188592259066 3.2523750887247923E-4 0.0 8.549100233219455E-5 8.363250228149466E-5 40-41 0.8680384676800894 3.438225093794781E-4 3.7170001013997627E-6 8.549100233219455E-5 8.734950238289442E-5 42-43 1.0279419535422565 3.6612450998787664E-4 3.7170001013997627E-6 9.292500253499407E-5 1.0035900273779359E-4 44-45 1.2225176163502804 3.8285101044417555E-4 3.7170001013997627E-6 1.003590027377936E-4 1.0779300294059313E-4 46-47 1.4535570501530364 4.107285112046738E-4 3.7170001013997627E-6 1.0407600283919336E-4 1.0779300294059313E-4 48-49 1.722916896501173 4.4418151211727164E-4 3.7170001013997627E-6 1.0407600283919336E-4 1.0779300294059313E-4 50-51 2.030866637902042 4.757760129791696E-4 3.7170001013997627E-6 1.0779300294059313E-4 1.0779300294059313E-4 52-53 2.3746668452809114 5.110875139424674E-4 3.7170001013997627E-6 1.0779300294059313E-4 1.189440032447924E-4 54-55 2.7527842560959543 5.48257514956465E-4 3.7170001013997627E-6 1.1336850309269277E-4 1.2080250329549229E-4 56-57 3.167668373413993 5.63125515362064E-4 3.7170001013997627E-6 1.1522700314339265E-4 1.2266100334619218E-4 58-59 3.618921478224178 5.687010155141638E-4 3.7170001013997627E-6 1.2266100334619218E-4 1.2823650349829183E-4 60-61 4.10511252548747 6.021540164267616E-4 3.7170001013997627E-6 1.2451950339689206E-4 1.3752900375179123E-4 62-63 4.623119235118693 6.188805168830605E-4 3.7170001013997627E-6 1.3195350359969157E-4 1.4682150400529062E-4 64-65 5.171876686588796 6.393240174407592E-4 3.7170001013997627E-6 1.3381200365039146E-4 1.5797250430948993E-4 66-67 5.751002028887335 6.634845180998577E-4 3.7170001013997627E-6 1.3381200365039146E-4 1.6540650451228944E-4 68-69 6.358430468457984 6.802110185561566E-4 3.7170001013997627E-6 1.4496300395459074E-4 1.802745049178885E-4 70-71 6.995332860332681 6.857865187082563E-4 3.7170001013997627E-6 1.5611400425879005E-4 1.8213300496858838E-4 72-73 7.65361915379073 6.913620188603558E-4 7.434000202799525E-6 1.5983100436018982E-4 1.9328400527278766E-4 74-75 8.334380288361896 7.099470193673547E-4 7.434000202799525E-6 1.6540650451228944E-4 2.007180054755872E-4 76-77 9.040993158638294 7.136640194687544E-4 7.434000202799525E-6 1.709820046643891E-4 2.081520056783867E-4 78-79 9.77078152454692 7.415415202292528E-4 7.434000202799525E-6 1.7284050471508896E-4 2.1558600588118625E-4 80-81 10.523903358592083 7.601265207362515E-4 7.434000202799525E-6 1.8770850512068803E-4 2.1558600588118625E-4 82-83 11.295403899638618 7.749945211418506E-4 7.434000202799525E-6 1.9328400527278766E-4 2.19303005982586E-4 84-85 12.08378705514571 7.954380216995493E-4 7.434000202799525E-6 1.9328400527278766E-4 2.19303005982586E-4 86-87 12.885724251522557 8.084475220544484E-4 7.434000202799525E-6 1.9514250532348755E-4 2.2302000608398576E-4 88 13.500623861297019 8.177400223079478E-4 7.434000202799525E-6 1.9700100537418743E-4 2.2673700618538553E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 189230 0.0 87.118546 8 GTATCAA 191175 0.0 86.18307 9 AGTGGTA 191065 0.0 86.08765 5 GTGGTAT 191950 0.0 85.86691 6 TGGTATC 191555 0.0 85.78652 7 AAGCAGT 201500 0.0 82.63635 1 GCAGTGG 201155 0.0 82.558945 3 CAGTGGT 200565 0.0 82.04528 4 AGCAGTG 207100 0.0 80.35611 2 CAACGCA 188245 0.0 44.92478 12-13 ATCAACG 189625 0.0 44.739025 10-11 ACGCAGA 190230 0.0 44.450165 14-15 TCAACGC 188335 0.0 44.333466 12-13 AACGCAG 189620 0.0 44.02811 14-15 AGAGTAC 189340 0.0 44.00556 18-19 GAGTACT 185045 0.0 43.970955 20-21 CGCAGAG 191870 0.0 43.470707 16-17 AGTACTT 192330 0.0 42.96321 20-21 TATCAAC 192915 0.0 42.87679 10-11 GTACTTT 193810 0.0 41.985767 22-23 >>END_MODULE