FastQCFastQC Report
Thu 26 May 2016
SRR1660767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26903416
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18262496.7881677181812154No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG717690.2667653802773596TruSeq Adapter, Index 5 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649100.2412704765818586No Hit
AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG400300.1487915140590325TruSeq Adapter, Index 5 (97% over 36bp)
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT315470.11726020219885831No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2246500.088.123588
AGTGGTA2280350.087.281225
TGGTATC2273850.087.047097
GTATCAA2272900.087.0400549
GTGGTAT2285300.086.942056
AAGCAGT2308400.084.367181
GCAGTGG2375600.084.0408253
CAGTGGT2372300.083.84674
AGCAGTG2422450.082.238942
CAACGCA2219850.045.21551512-13
ATCAACG2234800.044.97591810-11
ACGCAGA2244500.044.86491814-15
TCAACGC2209450.044.70408612-13
AACGCAG2234650.044.34537514-15
AGAGTAC2257000.044.3259118-19
GAGTACT2213550.044.11947620-21
CGCAGAG2262600.043.8079616-17
AGTACTT2289300.043.3597120-21
TATCAAC2282100.043.28280310-11
GTACTTT2295350.042.49959622-23
Produced by FastQC (version 0.11.5)