##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660767_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26903416 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.130982920533214 31.0 30.0 34.0 23.0 34.0 2 31.213696989259653 31.0 31.0 34.0 27.0 34.0 3 31.869658522174284 33.0 31.0 34.0 30.0 34.0 4 35.68576510878767 37.0 35.0 37.0 33.0 37.0 5 35.63532073399155 37.0 35.0 37.0 33.0 37.0 6 35.681077191089784 37.0 35.0 37.0 33.0 37.0 7 35.584231794207845 37.0 35.0 37.0 33.0 37.0 8 35.59593673903716 37.0 35.0 37.0 33.0 37.0 9 37.26687722481041 39.0 37.0 39.0 33.0 39.0 10-11 37.1310337505096 39.0 37.0 39.0 33.0 39.0 12-13 37.23773681751046 39.0 37.0 39.0 33.0 39.0 14-15 38.505649319774115 40.0 38.0 41.0 33.0 41.0 16-17 38.49369381196797 40.0 38.0 41.0 33.0 41.0 18-19 38.45581663309967 40.0 38.0 41.0 33.5 41.0 20-21 38.36340465091868 40.0 38.0 41.0 33.5 41.0 22-23 38.200157184500284 40.0 37.5 41.0 33.5 41.0 24-25 38.21774171726 40.0 38.0 41.0 33.0 41.0 26-27 38.16603293053938 40.0 38.0 41.0 33.0 41.0 28-29 38.057501415433634 40.0 37.5 41.0 33.0 41.0 30-31 37.98364575710386 40.0 37.0 41.0 33.0 41.0 32-33 37.87359123837656 40.0 37.0 41.0 33.0 41.0 34-35 37.76238305574281 40.0 37.0 41.0 32.0 41.0 36-37 37.56298315425818 39.5 37.0 41.0 32.0 41.0 38-39 37.07264698282181 39.0 35.0 41.0 31.0 41.0 40-41 36.84080298576211 39.0 35.0 40.5 31.0 41.0 42-43 36.683435962184134 39.0 35.0 40.0 30.0 41.0 44-45 36.58731974779708 38.5 35.0 40.0 30.0 41.0 46-47 36.67240306955816 39.0 35.0 40.5 30.5 41.0 48-49 36.61714872936582 39.0 35.0 41.0 30.0 41.0 50-51 36.43980667733793 39.0 35.0 40.0 30.0 41.0 52-53 36.15014760579103 38.5 35.0 40.0 29.0 41.0 54-55 35.64372619075585 39.0 34.5 40.5 26.5 41.0 56-57 35.10521825555536 38.0 34.0 40.0 23.5 41.0 58-59 34.85674692760206 38.0 34.0 40.0 23.0 41.0 60-61 34.66575231561672 38.0 34.0 40.0 23.0 41.0 62-63 34.40406075198777 37.0 34.0 40.0 22.5 41.0 64-65 34.12161232610758 37.0 33.0 40.0 22.0 41.0 66-67 33.88085470261471 36.0 33.0 39.5 22.0 41.0 68-69 33.647976803391806 36.0 33.0 39.0 22.0 41.0 70-71 33.309896390108975 35.0 32.5 39.0 22.0 40.5 72-73 32.963818739597976 35.0 32.0 38.0 21.0 40.0 74-75 32.601670992263585 35.0 32.0 37.0 20.0 39.5 76-77 30.559883938158634 33.5 29.0 35.5 18.0 38.5 78-79 31.80753369757952 35.0 31.0 36.0 20.0 39.0 80-81 31.819386486087865 35.0 32.0 36.0 19.5 38.5 82-83 31.63305481355974 35.0 32.0 36.0 19.0 37.0 84-85 31.40999527717967 35.0 32.0 35.0 19.0 37.0 86-87 31.21764892235246 35.0 31.5 35.0 18.0 36.5 88-89 31.036337913371298 35.0 31.0 35.0 18.0 36.0 90-91 30.892265911511014 35.0 31.0 35.0 17.5 36.0 92-93 30.768217575046975 35.0 31.0 35.0 17.0 35.5 94-95 30.64865181061022 35.0 31.0 35.0 17.0 35.0 96-97 30.552972715435097 34.0 31.0 35.0 15.5 35.0 98-99 30.433165030046744 34.0 31.0 35.0 15.0 35.0 100 30.291167634623054 34.0 31.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.3849212177399117 1101 2 1.5480765195963144 1101 3 1.0151243604835507 1101 4 0.6267621673436707 1101 5 0.6444146391741228 1101 6 0.529798840146654 1101 7 0.5204284237596184 1101 8 0.5669596490634916 1101 9 0.7000481943046424 1101 10-11 0.7606667394181628 1101 12-13 0.721132126305335 1101 14-15 0.926489924127246 1101 16-17 0.9160645996750745 1101 18-19 0.8954859492981342 1101 20-21 0.9265341510411389 1101 22-23 0.9527111058737034 1101 24-25 0.896527841935729 1101 26-27 0.8881710939679266 1101 28-29 0.9310888950042155 1101 30-31 0.8478632231573826 1101 32-33 0.8559733366023465 1101 34-35 0.8841449949262241 1101 36-37 0.9080218039208745 1101 38-39 1.0078017565079307 1101 40-41 1.06690750565663 1101 42-43 1.0051519668624778 1101 44-45 0.9975664788301515 1101 46-47 0.9318457421910296 1101 48-49 0.9016962243905624 1101 50-51 0.8992725493677511 1101 52-53 0.9970055836449205 1101 54-55 1.138825323789682 1101 56-57 1.157843874442264 1101 58-59 1.0866701054881602 1101 60-61 1.0855338437798494 1101 62-63 1.1068109394590167 1101 64-65 1.06536520429691 1101 66-67 1.0435810085507953 1101 68-69 0.9988232899342222 1101 70-71 1.0146937531120983 1101 72-73 0.9485403412383562 1101 74-75 0.8820436362533783 1101 76-77 1.1505739710091767 1101 78-79 1.0345806702837201 1101 80-81 0.9440374966203784 1101 82-83 0.8792230597412356 1101 84-85 0.8684603882503232 1101 86-87 0.8470360780692126 1101 88-89 0.8620037617748189 1101 90-91 0.8876917866867693 1101 92-93 0.8364766102481802 1101 94-95 0.8932921283581869 1101 96-97 0.8429506637633075 1101 98-99 0.8393892768237095 1101 100 0.759411725068464 1102 1 2.359901087785129 1102 2 1.5535594568613433 1102 3 0.967220427480104 1102 4 0.6210670131098652 1102 5 0.5888865577284861 1102 6 0.5156496066413467 1102 7 0.4785145423254704 1102 8 0.5518918777037314 1102 9 0.6213653116123865 1102 10-11 0.7057808208350522 1102 12-13 0.6280484527420711 1102 14-15 0.8398974467729587 1102 16-17 0.8294182028356119 1102 18-19 0.8130376714757119 1102 20-21 0.7960172675804174 1102 22-23 0.8236552737612328 1102 24-25 0.8143368525618158 1102 26-27 0.8921803319778192 1102 28-29 0.9018375067141804 1102 30-31 0.8817419421816339 1102 32-33 0.8197056079784346 1102 34-35 0.8388891785171566 1102 36-37 0.8659339001078834 1102 38-39 0.9881291998814135 1102 40-41 1.0200895478242131 1102 42-43 0.9487270195951965 1102 44-45 0.9554258975908425 1102 46-47 0.9026264528797014 1102 48-49 0.8121745698264533 1102 50-51 0.8629349377816169 1102 52-53 0.9240143276573889 1102 54-55 1.044269179718718 1102 56-57 1.0375393207902945 1102 58-59 0.945995140393805 1102 60-61 0.8995516985859027 1102 62-63 0.8938107496584919 1102 64-65 0.8903661821750717 1102 66-67 0.9720271269724776 1102 68-69 0.9352422241211542 1102 70-71 0.8655699514019517 1102 72-73 0.841600484288314 1102 74-75 0.823709985493629 1102 76-77 0.7244165052647098 1102 78-79 0.9105062212141313 1102 80-81 0.8522189557473006 1102 82-83 0.8298483897879123 1102 84-85 0.797527352814523 1102 86-87 0.8368635299824909 1102 88-89 0.845587684835241 1102 90-91 0.789147629169868 1102 92-93 0.7891730360717482 1102 94-95 0.7961229545757433 1102 96-97 0.8139221938082066 1102 98-99 0.8207578522307273 1102 100 0.7158584262850525 1103 1 1.9996351635404785 1103 2 1.2711463580864049 1103 3 0.7158736995766297 1103 4 0.5243691703325908 1103 5 0.46235038725622957 1103 6 0.45013138381808204 1103 7 0.49953021576273926 1103 8 0.5159897917752048 1103 9 0.6342554681766899 1103 10-11 0.5900407247835417 1103 12-13 0.6215486805410961 1103 14-15 0.7840750550933606 1103 16-17 0.7606260567969727 1103 18-19 0.7721523788185536 1103 20-21 0.7203084531507287 1103 22-23 0.7605733820620557 1103 24-25 0.7764192852448701 1103 26-27 0.7372307600959473 1103 28-29 0.8108365337169943 1103 30-31 0.7917194678710118 1103 32-33 0.7794610435329759 1103 34-35 0.7626873111871859 1103 36-37 0.7521122284004704 1103 38-39 0.8978534400191336 1103 40-41 0.934309654817497 1103 42-43 0.8621818011185027 1103 44-45 0.865791936051231 1103 46-47 0.8468921102754052 1103 48-49 0.7632757123620877 1103 50-51 0.7562030424585657 1103 52-53 0.8357564249644369 1103 54-55 0.9904340484961054 1103 56-57 0.9406028211569151 1103 58-59 0.8869434153781199 1103 60-61 0.8619046616634165 1103 62-63 0.8802357393909617 1103 64-65 0.8099952702582627 1103 66-67 0.8443075233937947 1103 68-69 0.7538134903175688 1103 70-71 0.7621019098675035 1103 72-73 0.7085361091612796 1103 74-75 0.7360034524212651 1103 76-77 0.704239353655737 1103 78-79 0.771738054717833 1103 80-81 0.7460083746375084 1103 82-83 0.7469421994617989 1103 84-85 0.7041658217787692 1103 86-87 0.663142254450154 1103 88-89 0.6264808020513897 1103 90-91 0.6901445020364534 1103 92-93 0.7128638124804709 1103 94-95 0.7925104407555672 1103 96-97 0.7348581623435102 1103 98-99 0.7688277684845772 1103 100 0.6179840671832011 1104 1 2.220584913795353 1104 2 1.444208662112942 1104 3 0.88889051589857 1104 4 0.5705637024705368 1104 5 0.47532053180762546 1104 6 0.4688023112121229 1104 7 0.5009519392741524 1104 8 0.5086667566679068 1104 9 0.5801495774083492 1104 10-11 0.6096372147442608 1104 12-13 0.5818167808356876 1104 14-15 0.7504243276266678 1104 16-17 0.7425734686406713 1104 18-19 0.6637872287344848 1104 20-21 0.7166118825323196 1104 22-23 0.7313789638797203 1104 24-25 0.7489340939040403 1104 26-27 0.7108333828897102 1104 28-29 0.7646577667528831 1104 30-31 0.7955402997411412 1104 32-33 0.7640085660933735 1104 34-35 0.6479504129672407 1104 36-37 0.7317769944459585 1104 38-39 0.8049870155531451 1104 40-41 0.8788194535072194 1104 42-43 0.8539629810036402 1104 44-45 0.7873194860614632 1104 46-47 0.8694719979077448 1104 48-49 0.8174865142460348 1104 50-51 0.8168692897105103 1104 52-53 0.912339045038074 1104 54-55 1.1016547743946035 1104 56-57 1.0730106182048615 1104 58-59 0.9843366341417763 1104 60-61 0.9159063900626805 1104 62-63 0.9337221217944673 1104 64-65 0.9163644398193682 1104 66-67 0.9216889270094413 1104 68-69 0.8866965357295697 1104 70-71 0.8415332894558745 1104 72-73 0.7903944838569785 1104 74-75 0.7941460242494216 1104 76-77 0.6872494334781258 1104 78-79 0.8607897662868176 1104 80-81 0.7886452629175231 1104 82-83 0.8408438255384496 1104 84-85 0.8230731280901189 1104 86-87 0.8572693611227002 1104 88-89 0.8410482757117066 1104 90-91 0.8497420133956908 1104 92-93 0.8373121371679666 1104 94-95 0.8523830789354605 1104 96-97 0.8457085627209189 1104 98-99 0.8952218364115865 1104 100 0.7933517707833992 1105 1 2.219469458148584 1105 2 1.3772017635861502 1105 3 0.8353689287642929 1105 4 0.5134388899091604 1105 5 0.44946392133300606 1105 6 0.39452024727268764 1105 7 0.4201314039007542 1105 8 0.44081792815940446 1105 9 0.5157622672282685 1105 10-11 0.5001175354321177 1105 12-13 0.4967196331385253 1105 14-15 0.637742489693558 1105 16-17 0.6475781627564956 1105 18-19 0.5788409264690699 1105 20-21 0.5150083408802928 1105 22-23 0.5859437878159355 1105 24-25 0.6411647041108992 1105 26-27 0.6642891874523045 1105 28-29 0.6416456575468956 1105 30-31 0.6230353235453663 1105 32-33 0.6659000222990841 1105 34-35 0.6036980569178496 1105 36-37 0.6965076919662678 1105 38-39 0.7792142696337692 1105 40-41 0.7869466807870964 1105 42-43 0.7038882186980615 1105 44-45 0.6944789840093364 1105 46-47 0.7180089356793573 1105 48-49 0.6967493523578554 1105 50-51 0.7151019688225517 1105 52-53 0.807754248515522 1105 54-55 0.8284175886326324 1105 56-57 0.7638849168503867 1105 58-59 0.7545896598992314 1105 60-61 0.6887695436005572 1105 62-63 0.7690397877900139 1105 64-65 0.7300943363047949 1105 66-67 0.7444370936324205 1105 68-69 0.6758944638168742 1105 70-71 0.6575651563756892 1105 72-73 0.7083851117705606 1105 74-75 0.6783514846224463 1105 76-77 0.7165728019277786 1105 78-79 0.7520159134123681 1105 80-81 0.7388438441816625 1105 82-83 0.7046099044522549 1105 84-85 0.7091623471546917 1105 86-87 0.6980402942284094 1105 88-89 0.704529836947156 1105 90-91 0.7368025751680456 1105 92-93 0.6724794847173676 1105 94-95 0.682113401943564 1105 96-97 0.6607324731819695 1105 98-99 0.6504873539934266 1105 100 0.5854557206730426 1106 1 2.1229598769247495 1106 2 1.276705390843702 1106 3 0.7254690036457809 1106 4 0.42670482036185575 1106 5 0.40991312462291773 1106 6 0.3463979427189159 1106 7 0.3632494778370514 1106 8 0.3651433827856536 1106 9 0.40806303612378514 1106 10-11 0.41166302247631137 1106 12-13 0.41010779836605593 1106 14-15 0.5179740877085024 1106 16-17 0.5107037885844079 1106 18-19 0.46818386601547957 1106 20-21 0.4924215196086337 1106 22-23 0.486587980430059 1106 24-25 0.5350387940246648 1106 26-27 0.554483005069315 1106 28-29 0.569516320963821 1106 30-31 0.561049471671268 1106 32-33 0.5643697988583725 1106 34-35 0.5251883419253929 1106 36-37 0.5453949211675635 1106 38-39 0.6018790271413366 1106 40-41 0.6982933579101314 1106 42-43 0.6069012137470509 1106 44-45 0.6130129162344033 1106 46-47 0.6340277144660575 1106 48-49 0.5768497807531006 1106 50-51 0.6315844834558817 1106 52-53 0.6294481472898141 1106 54-55 0.7726277999270152 1106 56-57 0.7802791623607064 1106 58-59 0.7042133478835595 1106 60-61 0.6783995298575505 1106 62-63 0.6439798836815456 1106 64-65 0.6994996784212759 1106 66-67 0.5933200332459592 1106 68-69 0.5918011174601929 1106 70-71 0.5593740167178325 1106 72-73 0.5646965118842999 1106 74-75 0.5634436736526638 1106 76-77 0.4497758463464443 1106 78-79 0.6345961792824291 1106 80-81 0.6147509049201041 1106 82-83 0.5830716641323122 1106 84-85 0.5688101181102887 1106 86-87 0.5908439225976174 1106 88-89 0.6119552904247918 1106 90-91 0.6298139944940928 1106 92-93 0.5601976343511872 1106 94-95 0.5935816502332791 1106 96-97 0.62537348595826 1106 98-99 0.6257266016705643 1106 100 0.49680629014276434 1107 1 2.184580235978398 1107 2 1.409050934101991 1107 3 0.854563324974901 1107 4 0.5149176335691763 1107 5 0.4455593496274943 1107 6 0.3835726461589317 1107 7 0.41623980319668874 1107 8 0.4562239142353306 1107 9 0.5389348408755765 1107 10-11 0.5373106183082044 1107 12-13 0.5484595176052096 1107 14-15 0.6927188502001584 1107 16-17 0.6410192097363989 1107 18-19 0.602871339986315 1107 20-21 0.6342417177384689 1107 22-23 0.645745395507376 1107 24-25 0.6755500667234813 1107 26-27 0.7199093333082587 1107 28-29 0.7510377069400462 1107 30-31 0.7273549986440457 1107 32-33 0.7003085815893968 1107 34-35 0.756192960248093 1107 36-37 0.7882197697867355 1107 38-39 0.8186167981178301 1107 40-41 0.8907173485980024 1107 42-43 0.8285966087417123 1107 44-45 0.8581952422472909 1107 46-47 0.9277806170167935 1107 48-49 0.8528543912701991 1107 50-51 0.7969575604955565 1107 52-53 0.834516123630678 1107 54-55 0.9037523236216458 1107 56-57 0.8407991723604411 1107 58-59 0.7880460005180652 1107 60-61 0.7530998142685021 1107 62-63 0.8270170592694157 1107 64-65 0.7450491173017539 1107 66-67 0.7535320622169266 1107 68-69 0.7335752814248622 1107 70-71 0.7089277500137214 1107 72-73 0.7362775098068042 1107 74-75 0.7381453133426632 1107 76-77 0.6603486366914062 1107 78-79 0.754059255299314 1107 80-81 0.7364974325856188 1107 82-83 0.6877381820141437 1107 84-85 0.6766522049600461 1107 86-87 0.7011876197933482 1107 88-89 0.7480262144378109 1107 90-91 0.721602471980777 1107 92-93 0.7374263234094549 1107 94-95 0.7615226317267485 1107 96-97 0.7622797509808947 1107 98-99 0.7896537020219938 1107 100 0.5988658157616058 1108 1 1.0370558582760445 1108 2 0.8279631182655507 1108 3 0.41892036605431926 1108 4 0.2496459482631721 1108 5 0.2024434198294287 1108 6 0.20542591718703562 1108 7 0.15908684417985341 1108 8 0.20807113352618245 1108 9 0.20434913646916186 1108 10-11 0.19087002595162517 1108 12-13 0.2101838466254975 1108 14-15 0.2984790321672435 1108 16-17 0.28911588665975785 1108 18-19 0.22284050694524637 1108 20-21 0.20322402660912786 1108 22-23 0.1983680839049029 1108 24-25 0.20465220844256038 1108 26-27 0.2598852780821872 1108 28-29 0.26047820346188644 1108 30-31 0.2687930501189726 1108 32-33 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2314 3 -1.996105725391267 2314 4 -1.1105243681781758 2314 5 -0.8412897011358069 2314 6 -0.7094839795281445 2314 7 -0.6034764886353585 2314 8 -0.5557546544528762 2314 9 -0.706792933561303 2314 10-11 -0.5496995808184053 2314 12-13 -0.5334009234843933 2314 14-15 -0.5974350085138767 2314 16-17 -0.6328134380528496 2314 18-19 -0.6750010863202789 2314 20-21 -0.7273762666112376 2314 22-23 -0.7121664224736151 2314 24-25 -0.6738038816274923 2314 26-27 -0.7485806291633637 2314 28-29 -0.7051636872724103 2314 30-31 -0.8492888689024909 2314 32-33 -0.7566877841789363 2314 34-35 -0.7274896072827701 2314 36-37 -0.6157062029761207 2314 38-39 -0.6399238479595226 2314 40-41 -4.139499637140226 2314 42-43 -1.4528853396808827 2314 44-45 -0.9850290759553175 2314 46-47 -0.7700581019196449 2314 48-49 -0.6928297525304217 2314 50-51 -0.6281307334809725 2314 52-53 -0.7849781900262585 2314 54-55 -0.774699748806988 2314 56-57 -0.8893161673805636 2314 58-59 -0.9265720089179226 2314 60-61 -0.9029528639185926 2314 62-63 -0.7673149558667447 2314 64-65 -0.8284034289296969 2314 66-67 -0.7011820276951539 2314 68-69 -0.7703785898589075 2314 70-71 -0.7177772243574907 2314 72-73 -0.6714145970724772 2314 74-75 -0.7890169738316644 2314 76-77 -0.6734923135061983 2314 78-79 -0.8037900863053373 2314 80-81 -0.7163577679522 2314 82-83 -0.6794693792584496 2314 84-85 -0.7404821517814746 2314 86-87 -0.69813534078909 2314 88-89 -0.8758949644509855 2314 90-91 -0.7631743185772777 2314 92-93 -0.737501429415989 2314 94-95 -0.6493109119886284 2314 96-97 -0.6812634733107181 2314 98-99 -0.6493771930247831 2314 100 -0.683766788751587 2315 1 1.0381778700688074 2315 2 0.33421672210424447 2315 3 -0.19323730441923814 2315 4 -0.3813817768023924 2315 5 -0.5090991764454103 2315 6 -0.5345009094777424 2315 7 -0.6064067418373895 2315 8 -0.6222709002704221 2315 9 -0.8158573066343919 2315 10-11 -0.7337671460042898 2315 12-13 -0.7167254181978748 2315 14-15 -0.9581101558455956 2315 16-17 -0.99126852809394 2315 18-19 -1.0191501206271951 2315 20-21 -0.8771926390327778 2315 22-23 -0.8726550845866683 2315 24-25 -0.9358873218077122 2315 26-27 -0.8788712751019361 2315 28-29 -1.056606734960269 2315 30-31 -1.0674994443791093 2315 32-33 -1.131233406091205 2315 34-35 -1.1099359418205381 2315 36-37 -1.0486713417223612 2315 38-39 -1.1600305437583103 2315 40-41 -0.9083001967399866 2315 42-43 -0.9190696903156947 2315 44-45 -1.0776850171796895 2315 46-47 -1.2155611849592844 2315 48-49 -1.204378321055799 2315 50-51 -1.323046591131643 2315 52-53 -1.2070059193034481 2315 54-55 -1.3710512962803136 2315 56-57 -1.300712174928563 2315 58-59 -1.3718598410327232 2315 60-61 -1.1389304223286771 2315 62-63 -1.417028228840877 2315 64-65 -1.5136034550238762 2315 66-67 -1.4310742481839185 2315 68-69 -1.479540108873664 2315 70-71 -1.2205954616790748 2315 72-73 -1.0355907721549755 2315 74-75 -0.9664773339908947 2315 76-77 -1.1184837454776861 2315 78-79 -1.2554776573847484 2315 80-81 -1.306472935744921 2315 82-83 -1.073894851172156 2315 84-85 -0.9697083889557057 2315 86-87 -0.9015796985672644 2315 88-89 -0.95846759137169 2315 90-91 -0.9530356136985318 2315 92-93 -0.8732657862769919 2315 94-95 -0.8767984255791674 2315 96-97 -0.8770362220836638 2315 98-99 -0.9430249768189789 2315 100 -1.7745970722337496 2316 1 0.9019090712260365 2316 2 0.19468171507821097 2316 3 -0.4396437908887414 2316 4 -0.4680382262834968 2316 5 -0.655073756783068 2316 6 -0.6086272039317535 2316 7 -0.7251850777586952 2316 8 -1.9635627245721992 2316 9 -1.2818699809799128 2316 10-11 -1.125718812127964 2316 12-13 -0.9874815462395361 2316 14-15 -1.43871437277582 2316 16-17 -1.3445628651816293 2316 18-19 -1.1629829856757326 2316 20-21 -1.2340242893611943 2316 22-23 -1.2748121103157075 2316 24-25 -1.3508232482436426 2316 26-27 -1.4161737001603711 2316 28-29 -1.7311070853395805 2316 30-31 -1.9527206559328931 2316 32-33 -1.7342808894105701 2316 34-35 -1.449452500248377 2316 36-37 -2.0400135708776403 2316 38-39 -2.0369459623921387 2316 40-41 -1.7258043289938314 2316 42-43 -1.6581924223175193 2316 44-45 -1.621787277258889 2316 46-47 -1.6740796052021238 2316 48-49 -1.6755721755387043 2316 50-51 -1.6320085187426656 2316 52-53 -1.4889960108692648 2316 54-55 -1.6246963572731303 2316 56-57 -2.435354022148836 2316 58-59 -2.0402542546923073 2316 60-61 -1.6204408612235284 2316 62-63 -1.8444306086965696 2316 64-65 -2.387889132266416 2316 66-67 -1.7979776366237772 2316 68-69 -1.556929630882422 2316 70-71 -1.767237406401975 2316 72-73 -1.6937735633655961 2316 74-75 -1.404559215172931 2316 76-77 -1.4605635345818442 2316 78-79 -1.2993599484260052 2316 80-81 -1.3218772861940415 2316 82-83 -1.4760080395869721 2316 84-85 -1.6003225364779148 2316 86-87 -1.2634012013893887 2316 88-89 -1.1519447781011003 2316 90-91 -1.0976248640182256 2316 92-93 -1.1198162114020924 2316 94-95 -1.2635777627678095 2316 96-97 -1.1768127547454448 2316 98-99 -1.2217457583165654 2316 100 -1.1377861306407624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 13.0 7 395.0 8 713.0 9 331.0 10 182.0 11 161.0 12 265.0 13 1162.0 14 4570.0 15 12047.0 16 22382.0 17 33593.0 18 47973.0 19 69730.0 20 99710.0 21 147042.0 22 223691.0 23 342628.0 24 501095.0 25 595942.0 26 574982.0 27 515493.0 28 508154.0 29 565200.0 30 644662.0 31 752735.0 32 906147.0 33 1121233.0 34 1454417.0 35 2043630.0 36 3172298.0 37 5260622.0 38 5820533.0 39 1457047.0 40 2638.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.715197677675267 35.721716639307424 27.52812924986174 19.034956433155564 2 22.015527693583596 33.97201307075652 25.55969100726837 18.452768228391516 3 27.719981730201106 25.892931217359166 25.707542120301753 20.679544932137986 4 20.302752631859093 26.493706226748305 25.419403989441342 27.78413715195126 5 20.71840616819812 33.33069302426131 25.12998721054605 20.820913596994522 6 28.958185086979288 25.59947405935365 25.103064978811613 20.339275874855446 7 21.012640179224825 25.66299387408647 32.96013041615235 20.364235530536344 8 28.17476040960746 26.271373865683078 25.15199928514654 20.401866439562916 9 28.042977888012437 25.990387243017764 25.382193101426225 20.58444176754357 10-11 20.389459093224442 30.10892743137154 29.231390169932325 20.270223305471692 12-13 20.45827749160181 25.911705784871337 29.13769017287619 24.492326550650667 14-15 20.17706784893041 33.977179700897466 25.212246281290078 20.63350616888205 16-17 24.178148975579905 26.059997362416727 25.220555635016755 24.54129802698661 18-19 20.512576286266786 29.750083367865898 25.438678151307652 24.298662194559665 20-21 24.220487378094514 29.758622376545134 25.40593776448666 20.614952480873693 22-23 24.41715389727118 26.17365959576729 29.080523014662752 20.328663492298784 24-25 20.446526195781235 29.98412171896684 25.06298828371832 24.506363801533602 26-27 20.41003268878569 25.827123589063934 33.11886304698258 20.643980675167793 28-29 20.392533052308302 26.149292714352708 32.86151654496217 20.596657688376823 30-31 20.67554729852893 25.87879174897344 33.0687169986146 20.376943953883032 32-33 20.405466354161124 26.165041643782338 32.78838085096703 20.64111115108951 34-35 20.421910362609715 26.228797488021595 32.73357182597184 20.61572032339685 36-37 20.481488298735 26.19847791819448 32.66233551902851 20.65769826404201 38-39 20.478128130643334 26.45768849576574 32.65728411589071 20.40689925770021 40-41 20.48096852313563 26.305689519934567 32.63650750959342 20.576834447336385 42-43 20.516940112955528 26.098753745591896 32.80028401929996 20.584022122152618 44-45 20.545801789209353 26.11594173266692 32.759092421417726 20.579164056706002 46-47 20.801327947552668 26.227748476878705 32.683678511960665 20.28724506360796 48-49 20.641494769344337 26.472520310153936 32.54460075049952 20.34138417000221 50-51 20.894244433494986 26.30319696205121 32.195378088789916 20.607180515663885 52-53 21.029007303143622 26.49609030110286 31.854001282996997 20.620901112756524 54-55 21.205753202492946 26.800434933615865 31.10506115654607 20.888750707345118 56-57 21.65210283752134 27.408415884826496 29.999198986299508 20.94028229135266 58-59 21.75496784497552 28.03852678039101 28.90726590259021 21.29923947204325 60-61 22.017120428127047 28.38161332375041 28.200783127317365 21.40048312080518 62-63 21.7621090978511 28.580057589729897 28.485510088535992 21.172323223883012 64-65 21.96372572167044 28.95101685228374 27.928520303890036 21.15673712215579 66-67 21.67123684219134 29.029295015919164 28.068850067218232 21.230618074671263 68-69 21.546576092790595 28.87998683884604 28.30385182312908 21.269585245234286 70-71 21.461030667782957 28.75894514792399 28.528615626895203 21.25140855739785 72-73 21.391807077405222 28.63304666686813 28.6079494588619 21.36719679686475 74-75 21.40258993382293 28.662428917081208 28.455794810546188 21.479186338549678 76-77 21.435055872216292 28.751484686962502 28.26365040833038 21.54980903249082 78-79 21.513805871710804 28.915549169726003 27.79964589868889 21.770999059874306 80-81 21.538938573050302 29.11831510285304 27.396585790480582 21.946160533616084 82-83 21.483250389049324 29.388908859515567 27.0144655688699 22.11337518256521 84-85 21.47646185830128 29.51746169263435 26.72131085997122 22.284765589093148 86-87 21.444911139853513 29.59386311210469 26.50186632940003 22.459359418641764 88-89 21.458349517847072 29.677574750151376 26.28749866651997 22.576577065481583 90-91 21.44504682787233 29.683440497946073 26.09067135406919 22.780841320112412 92-93 21.377417239853152 29.749518803615487 25.983814896464025 22.88924906006734 94-95 21.37475369430891 29.806050048160436 25.87413372404615 22.9450625334845 96-97 21.370574519120332 29.786044918330777 25.765129944151305 23.078250618397583 98-99 21.317828146325766 29.743294708266905 25.717688743565343 23.221188401841985 100 21.276416976031502 29.7804071861029 25.665452699544083 23.277723138321516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 43711.0 1 28304.0 2 10217.0 3 7138.5 4 6725.5 5 6880.5 6 7226.5 7 7427.5 8 7587.5 9 7923.0 10 8644.0 11 11642.5 12 118216.0 13 146904.5 14 69393.5 15 72547.5 16 83157.5 17 94123.5 18 104705.0 19 114620.5 20 126132.0 21 141355.0 22 161493.5 23 184873.0 24 208351.5 25 234734.5 26 265367.5 27 301737.0 28 345246.5 29 387779.0 30 424100.5 31 460647.5 32 498845.0 33 534154.5 34 566544.0 35 602929.5 36 644763.0 37 684968.0 38 726838.5 39 758892.0 40 777766.5 41 804995.0 42 837358.0 43 852337.5 44 859327.0 45 875493.0 46 888153.0 47 893572.5 48 897729.0 49 900135.0 50 896650.5 51 889617.0 52 874428.5 53 837369.0 54 788432.0 55 739793.0 56 686235.5 57 623424.5 58 553308.0 59 476892.5 60 395510.5 61 320081.0 62 253927.0 63 194483.0 64 146636.0 65 109726.0 66 81018.5 67 60162.5 68 45075.0 69 34029.0 70 26119.0 71 20963.5 72 17406.5 73 13589.5 74 10268.0 75 8241.5 76 6312.5 77 4084.5 78 2543.5 79 1703.5 80 1133.0 81 805.0 82 527.5 83 337.0 84 241.0 85 158.0 86 101.0 87 64.5 88 43.0 89 33.0 90 27.0 91 21.0 92 14.5 93 10.0 94 3.0 95 2.5 96 2.5 97 1.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10510561186728108 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.397465065402847E-4 20-21 0.008355816227946667 22-23 7.434000202799525E-6 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.004685278627814401 42-43 0.03519627396015435 44-45 0.012139722331171625 46-47 0.0031501575859362993 48-49 3.7170001013997627E-6 50-51 0.0 52-53 5.575500152099644E-6 54-55 0.0 56-57 2.2302000608398576E-5 58-59 0.0 60-61 0.0 62-63 2.97360008111981E-5 64-65 0.0 66-67 0.0 68-69 0.0 70-71 1.486800040559905E-5 72-73 9.292500253499407E-5 74-75 2.397465065402847E-4 76-77 6.504750177449584E-5 78-79 3.6612450998787664E-4 80-81 1.0779300294059313E-4 82-83 5.575500152099645E-5 84-85 1.4496300395459074E-4 86-87 2.713410074021827E-4 88-89 4.683420127763701E-4 90-91 3.7541701024137607E-4 92-93 5.910030161225623E-4 94-95 4.07011511103274E-4 96-97 4.460400121679715E-5 98-99 2.787750076049822E-4 100 0.10878544196766685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2.6903416E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.57981653455943 #Duplication Level Percentage of deduplicated Percentage of total 1 74.8884868509669 34.882919780688034 2 13.109911695749703 12.213145631445922 3 4.436252227210444 6.1991944453347925 4 2.1835458876922798 4.0683666737399236 5 1.2161918153230729 2.832499581429075 6 0.861609186786818 2.4080158707012553 7 0.5873254254847313 1.9150257395612584 8 0.4269116234858158 1.5908372078752169 9 0.3160857768993787 1.3250895747441067 >10 1.7500969598566403 14.670365279128369 >50 0.15787531863084675 5.075175281699332 >100 0.06476004526565125 4.527978480934131 >500 3.5721281841681184E-4 0.10806522504858587 >1k 5.193169723484626E-4 0.5163997091834288 >5k 7.850761887131392E-6 0.02708278280147433 >10k+ 6.280609509704742E-5 7.63983873568512 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1826249 6.7881677181812154 No Hit AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAAACATCTCGTATG 71769 0.2667653802773596 TruSeq Adapter, Index 5 (97% over 36bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64910 0.2412704765818586 No Hit AGATCGGAAGAGCACACGTCTGAAGTCCAGTCACACAAACATCTCGTATG 40030 0.1487915140590325 TruSeq Adapter, Index 5 (97% over 36bp) TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 31547 0.11726020219885831 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.47723679401901975 3.7170001013997627E-6 0.0 0.0 7.434000202799525E-6 2 0.47734830402206174 2.2302000608398576E-5 0.0 0.0 7.434000202799525E-6 3 0.4774523800249009 2.97360008111981E-5 0.0 7.434000202799525E-6 1.1151000304199288E-5 4 0.47750441802632054 3.3453000912597864E-5 0.0 7.434000202799525E-6 1.486800040559905E-5 5 0.4775676070280443 4.088700111539739E-5 0.0 7.434000202799525E-6 1.486800040559905E-5 6 0.4776419470300723 4.460400121679715E-5 0.0 1.1151000304199288E-5 1.486800040559905E-5 7 0.4776791170310863 5.575500152099644E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 8 0.4777200040322017 6.318900172379597E-5 0.0 1.486800040559905E-5 1.486800040559905E-5 9 0.4777943440342297 7.06230019265955E-5 0.0 1.8585000506998813E-5 1.486800040559905E-5 10-11 0.4778965615370182 8.363250228149466E-5 0.0 2.2302000608398576E-5 1.6726500456298932E-5 12-13 0.4780192225403644 9.664200263639383E-5 0.0 2.601900070979834E-5 2.2302000608398576E-5 14-15 0.47825711054685394 1.1522700314339264E-4 0.0 3.3453000912597864E-5 2.2302000608398576E-5 16-17 0.4784039320508593 1.2266100334619218E-4 3.7170001013997627E-6 3.3453000912597864E-5 2.601900070979834E-5 18-19 0.47860279155628416 1.3381200365039146E-4 3.7170001013997627E-6 3.3453000912597864E-5 2.97360008111981E-5 20-21 0.4787533300603909 1.4682150400529062E-4 3.7170001013997627E-6 3.717000101399763E-5 4.274550116609727E-5 22-23 0.4790135200674888 1.5239700415739028E-4 3.7170001013997627E-6 4.460400121679715E-5 5.017950136889679E-5 24-25 0.4797085990864506 1.5611400425879005E-4 3.7170001013997627E-6 4.460400121679715E-5 5.761350157169632E-5 26-27 0.48171763764125713 1.8770850512068803E-4 3.7170001013997627E-6 4.646250126749704E-5 6.690600182519573E-5 28-29 0.48728012829300194 2.1186900577978648E-4 3.7170001013997627E-6 5.203800141959668E-5 6.690600182519573E-5 30-31 0.5009140846649363 2.211615060332859E-4 3.7170001013997627E-6 5.761350157169632E-5 7.06230019265955E-5 32-33 0.5265353663638848 2.397465065402847E-4 3.7170001013997627E-6 6.133050167309609E-5 7.434000202799525E-5 34-35 0.571250877583724 2.527560068951839E-4 3.7170001013997627E-6 6.318900172379597E-5 8.363250228149466E-5 36-37 0.6389653269309741 2.7691650755428234E-4 3.7170001013997627E-6 6.504750177449586E-5 9.292500253499407E-5 38-39 0.7352653655580392 2.843505077570819E-4 3.7170001013997627E-6 6.690600182519573E-5 9.478350258569396E-5 40-41 0.8624127880266208 3.122280085175801E-4 3.7170001013997627E-6 6.690600182519573E-5 1.003590027377936E-4 42-43 1.0225392938948719 3.3638850917667854E-4 3.7170001013997627E-6 6.690600182519573E-5 1.0221750278849347E-4 44-45 1.2170907962022368 3.512565095822776E-4 3.7170001013997627E-6 6.876450187589561E-5 1.0779300294059313E-4 46-47 1.4483532500110767 3.698415100892764E-4 3.7170001013997627E-6 7.619850207869514E-5 1.1151000304199288E-4 48-49 1.7181182493702658 3.8285101044417555E-4 3.7170001013997627E-6 8.549100233219455E-5 1.1336850309269277E-4 50-51 2.0263988037801592 4.032945110018743E-4 3.7170001013997627E-6 1.0221750278849347E-4 1.189440032447924E-4 52-53 2.370955420679664 4.1072851120467377E-4 3.7170001013997627E-6 1.0407600283919336E-4 1.189440032447924E-4 54-55 2.7510000960472825 4.3488901186377227E-4 3.7170001013997627E-6 1.1151000304199288E-4 1.2080250329549229E-4 56-57 3.16885595494639 4.5719101247217083E-4 3.7170001013997627E-6 1.1151000304199288E-4 1.3195350359969157E-4 58-59 3.6238111918575693 4.906440133847687E-4 3.7170001013997627E-6 1.189440032447924E-4 1.3381200365039146E-4 60-61 4.113815881224897 5.185215141452668E-4 3.7170001013997627E-6 1.2451950339689206E-4 1.3567050370109134E-4 62-63 4.635704997462032 5.352480146015658E-4 3.7170001013997627E-6 1.3195350359969157E-4 1.4496300395459074E-4 64-65 5.188320695037389 5.445405148550653E-4 3.7170001013997627E-6 1.3381200365039146E-4 1.4496300395459074E-4 66-67 5.771902720457506 5.519745150578648E-4 5.575500152099644E-6 1.3381200365039146E-4 1.5425550420809016E-4 68-69 6.384479205168593 5.687010155141638E-4 7.434000202799525E-6 1.3381200365039146E-4 1.5797250430948993E-4 70-71 7.02786218672008 5.817105158690628E-4 7.434000202799525E-6 1.3381200365039146E-4 1.6726500456298933E-4 72-73 7.693259101372108 6.002955163760617E-4 7.434000202799525E-6 1.4682150400529062E-4 1.7284050471508896E-4 74-75 8.382104711163816 6.281730171365598E-4 7.434000202799525E-6 1.486800040559905E-4 1.7655750481648872E-4 76-77 9.097270398673537 6.448995175928588E-4 7.434000202799525E-6 1.5425550420809016E-4 1.802745049178885E-4 78-79 9.834922078296675 6.523335177956583E-4 7.434000202799525E-6 1.5611400425879005E-4 1.8585000506998815E-4 80-81 10.595108071034549 6.690600182519573E-4 7.434000202799525E-6 1.598310043601898E-4 1.9885950542488731E-4 82-83 11.374319528791437 6.876450187589562E-4 7.434000202799525E-6 1.598310043601898E-4 2.007180054755872E-4 84-85 12.17056599801304 7.043715192152551E-4 7.434000202799525E-6 1.598310043601898E-4 2.007180054755872E-4 86-87 12.980777236615603 7.266735198236537E-4 7.434000202799525E-6 1.6354800446158956E-4 2.007180054755872E-4 88 13.601834057058033 7.322490199757533E-4 7.434000202799525E-6 1.6354800446158956E-4 2.1558600588118625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 224650 0.0 88.12358 8 AGTGGTA 228035 0.0 87.28122 5 TGGTATC 227385 0.0 87.04709 7 GTATCAA 227290 0.0 87.040054 9 GTGGTAT 228530 0.0 86.94205 6 AAGCAGT 230840 0.0 84.36718 1 GCAGTGG 237560 0.0 84.040825 3 CAGTGGT 237230 0.0 83.8467 4 AGCAGTG 242245 0.0 82.23894 2 CAACGCA 221985 0.0 45.215515 12-13 ATCAACG 223480 0.0 44.975918 10-11 ACGCAGA 224450 0.0 44.864918 14-15 TCAACGC 220945 0.0 44.704086 12-13 AACGCAG 223465 0.0 44.345375 14-15 AGAGTAC 225700 0.0 44.32591 18-19 GAGTACT 221355 0.0 44.119476 20-21 CGCAGAG 226260 0.0 43.80796 16-17 AGTACTT 228930 0.0 43.35971 20-21 TATCAAC 228210 0.0 43.282803 10-11 GTACTTT 229535 0.0 42.499596 22-23 >>END_MODULE