FastQCFastQC Report
Thu 26 May 2016
SRR1660766_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660766_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28563232
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15115155.291820617498748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1478800.5177285259595272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1893650.087.042978
GTATCAA1917150.085.8535169
AGTGGTA1923050.085.7763445
GTGGTAT1923650.085.758756
TGGTATC1924750.085.453517
AAGCAGT2015250.082.224721
CAGTGGT2010400.082.012064
GCAGTGG2025050.081.955823
AGCAGTG2097150.079.244212
CAACGCA1861900.044.9472912-13
ATCAACG1883000.044.54091610-11
TCAACGC1858700.044.46492812-13
ACGCAGA1884850.044.41076714-15
AACGCAG1874800.044.1525814-15
AGAGTAC1879100.044.05119718-19
GAGTACT1841250.043.87919620-21
CGCAGAG1903100.043.44042216-17
TATCAAC1923400.042.79215210-11
AGTACTT1917350.042.6870320-21
GTACTTT1924150.041.9660422-23
Produced by FastQC (version 0.11.5)