FastQCFastQC Report
Thu 26 May 2016
SRR1660766_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660766_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28563232
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16482095.770386908596338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1148610.40212886272813947No Hit
TAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310490.10870268462616556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2050200.087.216778
GTGGTAT2085850.086.0685656
GTATCAA2075800.085.98279
AGTGGTA2090150.085.9640665
TGGTATC2083650.085.870767
CAGTGGT2161750.082.918994
AAGCAGT2145750.082.265411
GCAGTGG2189400.082.230283
AGCAGTG2254350.079.746482
CAACGCA2022450.044.91309412-13
ATCAACG2041250.044.53397410-11
ACGCAGA2050350.044.40867614-15
TCAACGC2016150.044.37167412-13
AACGCAG2031900.044.1063814-15
AGAGTAC2059000.043.98201418-19
GAGTACT2016550.043.8360620-21
CGCAGAG2068400.043.41554616-17
TATCAAC2084050.042.8414510-11
AGTACTT2100400.042.7652520-21
GTACTTT2099900.042.0940322-23
Produced by FastQC (version 0.11.5)