FastQCFastQC Report
Thu 26 May 2016
SRR1660765_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660765_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33781795
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15915664.711312705556351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250500.37016979115526577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2015900.085.585888
GTGGTAT2044200.084.502016
AGTGGTA2058550.083.958835
TGGTATC2053300.083.850827
GTATCAA2057300.083.7425849
GCAGTGG2159650.080.5850753
AAGCAGT2160250.080.451061
CAGTGGT2159450.080.01634
AGCAGTG2258250.077.110732
CAACGCA1975350.044.50247212-13
ATCAACG2003150.043.97748610-11
ACGCAGA2005450.043.85781514-15
TCAACGC1971850.043.85729612-13
AACGCAG1982250.043.7156814-15
AGAGTAC2010500.043.3583418-19
GAGTACT1967650.043.11011520-21
CGCAGAG2019600.042.86826316-17
TATCAAC2046650.042.07196410-11
AGTACTT2063300.041.82958220-21
GTACTTT2059700.041.20219822-23
Produced by FastQC (version 0.11.5)