FastQCFastQC Report
Thu 26 May 2016
SRR1660765_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660765_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33781795
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17367315.14102640194223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942620.27903194605260023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200200.085.9730158
GTGGTAT2233250.085.0163356
AGTGGTA2247700.084.499415
TGGTATC2239500.084.462227
GTATCAA2243250.084.172299
GCAGTGG2321700.081.852713
AAGCAGT2291450.081.1724551
CAGTGGT2339200.081.013324
AGCAGTG2419300.078.430142
CAACGCA2151550.044.5886312-13
ATCAACG2173000.044.24797410-11
ACGCAGA2189200.043.94587314-15
TCAACGC2144250.043.93721412-13
AACGCAG2158750.043.70352614-15
AGAGTAC2202600.043.53832218-19
GAGTACT2153100.043.35058220-21
CGCAGAG2200000.042.96206316-17
AGTACTT2253800.042.23327320-21
TATCAAC2232400.042.2317510-11
GTACTTT2259700.041.32206722-23
Produced by FastQC (version 0.11.5)